Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	339,941	C→T	T58I (ACT→ATT)	yahH →	putative uncharacterized protein YahH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	339,941	0	C	T	100.0%	38.5 / NA	20	T58I (ACT→ATT)	yahH	putative uncharacterized protein YahH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/15);  total (5/15)

CGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGG‑CATTTGCCACCACTGCCGGATGCGGCGTGGACGCCTTAT‑CCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTG  >  NC_000913/339701‑340186                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                       cGAGAAAGCCATTCTTGGCTGGGGCGAACGTTACGGCGTCTGTCCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATTCGGCGTGAACGCCTTAGCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGG‑CATTTGTCACCATTGCCGGATgc                                                                                                                                                                                                                                              <  1:388845/251‑1 (MQ=255)  gagaAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCCTTGCCGGGTGCGGCGTGGACGCCTTTTTCCGCCCACGAAAGGCGCAAGAATCTTTAGGCCTGGTAAACGTCGCGCCACAGGGCTTTTGC‑ACCATTGCCGGATgcg                                                                                                                                                                                                                                             >  2:87053/1‑251 (MQ=18)                 ggCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGG‑CATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTa                                                                                                                                                                                                                               <  1:116989/250‑1 (MQ=255)                         cGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAAGCCTGGTAAGCGTAGCGCATAAGG‑CATTTGTCCCCATTTCCCGGTGCGGCGTGAACCCCTTATACCGGct                                                                                                                                                                                                                       >  1:310666/1‑249 (MQ=17)                                          gacctgcagggtCATGCCGGGTGCGGCGGGTACGTCTACACCGGGCTACGAGTGGGTCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCGCCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAAGCTGTAGGCCTGATAAGCGTAGCGCATCAGG‑CATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTAT‑CCGGCCTACGAGTGGCGCGAGaagt                                                                                                                                                                                                    <  2:311132/240‑3 (MQ=11)                                          gACCTACATGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGG‑CATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTAT‑CCGGCCTACGAGTGGCGCGAGAAtt                                                                                                                                                                                                    <  1:185644/251‑2 (MQ=255)                                            ccTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGG‑CATTTGTCACCATTGCCGGATGCGGCGTGGACGCCTTAT‑CCGGCCTaagcgaggcgcggggattgt                                                                                                                                                                                                  >  1:253670/1‑232 (MQ=14)                                                              aTGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGAGGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGG‑CATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTAT‑CCGGCCTACGAGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAgc                                                                                                                                                                               <  1:133914/251‑1 (MQ=9)                                                                gcggcgTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGG‑CATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTAT‑CCGGCCTACGAGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAgcgc                                                                                                                                                                             <  1:327644/251‑1 (MQ=9)                                                                                          cGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGAAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGG‑CATTTGTAACCATTGCCGGATGCGGCGTGAACGCCTTAT‑CCGGCCTACGCGGGGCGCGAGAAT‑TGGAGGCCTGATAAGGGGAGCGCATCAGGCaggcgtgcgttgggatta                                                                                                                                                    >  1:362022/1‑233 (MQ=11)                                                                                                                                                                               ccGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGG‑CATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTAT‑CCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGtt                                                               <  1:235286/251‑1 (MQ=255)                                                                                                                                                                                 ggCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGG‑CATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTAT‑CCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGGTgg                                                             >  1:157721/1‑251 (MQ=255)                                                                                                                                                                                                  aGAATCTGTAGGCCTGATAAGCGTAGCGCTTCAGG‑CATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTAC‑CCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCAccc                                            <  2:157721/251‑1 (MQ=14)                                                                                                                                                                                                   gAATCTGGAGGCCGGATAAGCGTAGCTCATCAGG‑CATTTGTCACCATTGCCGGATGCGGCGGGAACGCCTTAT‑CCGGCTTACGAATGTCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCCGGCAGTTGTCACCATTGCCGGATGCGGCGTGAACGTCTTATCCGGCCTACTAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCTAATCAGGCAGTCTGGCGTTGGTCATAACCCCATCAcccg                                           <  2:139040/251‑2 (MQ=14)                                                                                                                                                                                                           aGGCCTGATAAGCGTAGCGCATCAGG‑CATTTGTCTCCATTGCCGGATGCGGCGTGAACGCCTTAT‑CCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGc                                    <  1:113012/250‑1 (MQ=255)                                                                                                                                                                                                            ggCCTGATAAGCGTAGCGCATCAGG‑CATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTAT‑CCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCgg                                  <  1:171709/251‑1 (MQ=255)                                                                                                                                                                                                             gCCTGATAAGCGTAGCGCATCAGT‑CATTTGTCACCATTGCCGTATGCGGCGTGAACGCCTTAT‑CCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGa                                 <  1:79882/251‑1 (MQ=14)                                                                                                                                                                                                                 gATAAGCGTAGCGCATCAGG‑CATTTGTCCCCATTGCCGGATGCGGCGTGACCGCCTTAT‑CCGGCCTACGAAGGGCGCAAGATGCTTTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTTATAACCCCAGCACCCTCTGTAGCGGACATa                             <  2:248545/251‑1 (MQ=14)                                                                                                                                                                                                                  aTAAGCGTAGCGCATCAGG‑CATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTAT‑CCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATaa                            <  1:126641/251‑1 (MQ=255)                                                                                                                                                                                                                    aaGCGTAGCGCATCAGG‑CATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTAT‑CCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATaaca                          <  1:78326/251‑1 (MQ=255)                                                                                                                                                                                                                                             caccatTGCCGGATGCGGCGTGAACGCCTTAT‑CCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTg  <  1:251254/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                       CGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGG‑CATTTGCCACCACTGCCGGATGCGGCGTGGACGCCTTAT‑CCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTG  >  NC_000913/339701‑340186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑