Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,143,377	C→T	S283L (TCA→TTA)	frwC →	putative PTS enzyme IIC component FrwC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,143,377	0	C	T	100.0%	134.4 / NA	46	S283L (TCA→TTA)	frwC	putative PTS enzyme IIC component FrwC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/28);  total (18/28)

ATGCCTTCATGCTGATTTGCGTTGCTCAGGGTGTTTATACCGTGGTGGCCATCGCTGCCGTGGGTATTTGTATCCCGCCGCTGGGGATGGGGCTGGCGACGCTGATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTT‑GGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTCAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGG  >  NC_000913/4143131‑4143615                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               aTGCCTTCATTCTGATTTGCGTTGCTCAGGGTGTTTAGCCCGTGGGGGCCATCGCTGCTGTGGTTATTTGTATCCCGCCGCTGGGGAGGGGGCTGGCGACGCTGATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTT‑GGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCTGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGt                                                                                                                                                                                                                                             <  1:2362/250‑1 (MQ=255)      ttCATGCTGATTTGCGTTGCTCAGGGTGTTTATACCGTGGTGGCCATCGCTGCCGTGGGTATTTGTATCCCGCCGCTGGGGATGGGGCTGGCGACGCTGATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTT‑GGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTgtgg                                                                                                                                                                                                                                       >  2:335005/1‑251 (MQ=255)          tGCTGATTTGCGTTGCTCAGGGTGTTTATACCGTGGTGGCCATCGCTGCCGTGGGTATTTGTATCCCGCCGCTGGGGATGGGGCTGGCGACGCTGATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTT‑GGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGc                                                                                                                                                                                                                                    >  1:19374/1‑250 (MQ=255)                        gCTCAGGGTGTTTATACCGTGGTGGCCATCGCTGCCGTGGGTATTTGTATCCCGCCGCTGGGGATGGGGCTGGCGACGCTGATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTT‑GGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCt                                                                                                                                                                                                                     >  1:325351/1‑251 (MQ=255)                        gCTCAGGGAGTATATACCGTGGTGGCCATCGCTGCCGTGGGTATTTGTATCCCGCCGCTGGGGATGGGGCTGGCGACGCTGATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTT‑GGCGTTACTGTAGGGGCGATTCCTTTCGCCGCTCCCGATCCGCTGCGTTTCATTACTTCCAACATGGTCGGCTTAGTTTGTGGTGGAGTAACTGAGGCGCt                                                                                                                                                                                                                     >  2:371529/1‑251 (MQ=255)                            aGGGTGTTTATACCGTGGTGGCCATCGCTGCCGTGGGTATTTGTATCCCGCAGCTGGGGATGGGGCTGGCGACGCTGATTGGTCGTAAAAATTTGTCCGCAGAAGAGCGGGAAACCGGCAACGCGGCACTGGTGATGGGCTGTGTT‑GGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGGCGATCCGCTGCGGGACATTCCTTCCATCATGGTCGGTTTCGGTTGGGGTGCCGTACATGCGGGGCTGGTc                                                                                                                                                                                                                 >  2:108868/1‑251 (MQ=255)                                 gtTTATACCGTGGTGGCCATCGCTGCCGTGGGTATTTGTATCCCGCCGCTGGGGATGGGGCTGGCGACGCTGATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTT‑GGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTgc                                                                                                                                                                                                            <  1:335005/251‑1 (MQ=255)                                       aCCGTGGTGGCCATCGGTGACGTGGGTATTTGTATCCCGCAGCTGGGGAGGGGGATGGCGACGCTGATTGGTAGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCACAGCGGCACTGGTGATGGGCTGTGTT‑GGAGTTACTGAAGGGGCGATTCGTTTCGCCGTTGCCGCTCCGCTGCGTGTCAGTCGGTCCATCATGTTCGGTATAGTTTGTGGTGGTGTACCTGCGGCGCTGGTCGGTgcggcgtg                                                                                                                                                                                                      >  2:183532/1‑246 (MQ=255)                                        ccGTGGTGGCCATCGCTGCCGTGGGTATTTGTATCCCGCCGCTGGGGATGGGGCTGGCGACGCTGATTGGTCGTAAAAATTTCTCAGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTT‑GGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGc                                                                                                                                                                                                     >  1:268439/1‑251 (MQ=255)                                         cGTGGTGGCCATCGCTGCCGTGGGTATTTGTATCCCGCCGCTGGGGCTGGGGCTGGCGACGCTGATTGGTCGTAAACATTTCTCCGCAGAAGCGCGCGAACCCGGCAAAGCGGCACTGGTGATGGGCTGTGTT‑GGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGACGCTGGTCGGTgcgacgggct                                                                                                                                                                                                    >  2:184679/1‑244 (MQ=255)                                                gcctccgcggcctggggTTTTTGTACCCCGCCGCTGGGGATGGGGGTGGCGACGCTGATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTT‑GGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAgg                                                                                                                                                                                             <  2:416458/237‑1 (MQ=255)                                                 ccATCGCTGCCGTGGGTATTTGTATCCCGCCGCTGGGGATGGGGCTGGCGACGCTGATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTT‑GGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGc                                                                                                                                                                                            <  1:74587/251‑1 (MQ=255)                                                   aTCGCTGCCGTGGGTATTTGTATCCCGCCGCTGGGGATGGGGCTGGCGACGCTGATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTT‑GGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTg                                                                                                                                                                                          <  1:297951/251‑1 (MQ=255)                                                                                    gggATGGGGCTGGCGACGCTGATTGGACGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTT‑GGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAgg                                                                                                                                                         >  1:325806/1‑251 (MQ=255)                                                                                           ggCTGGCGACGCTGATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTT‑GGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTg                                                                                                                                                  <  2:19374/251‑1 (MQ=255)                                                                                                   aCGCTGATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTT‑GGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATACGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTatat                                                                                                                                          >  2:49638/1‑251 (MQ=255)                                                                                                     gCTGATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTT‑GGCGTTTCTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCg                                                                                                                                        <  2:170705/251‑1 (MQ=255)                                                                                                       tGATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTT‑GGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTTCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCgc                                                                                                                                       >  1:113530/1‑250 (MQ=255)                                                                                                        gATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCTGAAAAGCGGCACTGGTGATGGGCTGTGTT‑GGCGTTACTGAAGGGGCGATTCCTTTCGTCGCTGCCGATCCGCTGCGGGTCATTCCTTCCATCAGGGGCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCGGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCgc                                                                                                                                       <  2:387676/249‑1 (MQ=255)                                                                                                            ggTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTT‑GGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGcagcag                                                                                                                                  <  1:49638/250‑1 (MQ=255)                                                                                                                  aaaaaTTTCTCCGCAGAAGAGCGCGAAACCGGCAACGCGGCACTGTGGATGGG‑TGGGTTTGGCGTTACTGTAGGGGCGATTCCTTTCGCCGCGGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGGCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCGGCCGGTGGTTTAATGCAAGCTGGGTTATATCGCATCAGTGGCTGt                                                                                                                           <  2:363801/251‑1 (MQ=255)                                                                                                                           cccGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTT‑GGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAgt                                                                                                                  <  2:268925/250‑1 (MQ=255)                                                                                                                              gCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTT‑GGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTACCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGTAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAGGGCAAGCTGGGTTATATCGCAGCAGTGGCTGGCGGAGCAgtggg                                                                                                               >  1:251288/1‑250 (MQ=255)                                                                                                                                agaagaGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTT‑GGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAgtggtgg                                                                                                             >  1:228402/1‑250 (MQ=255)                                                                                                                                      gcgcGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGGT‑GGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTTTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTg                                                                                                       <  2:355299/251‑1 (MQ=255)                                                                                                                                         cgAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTT‑GGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGttt                                                                                                    <  1:397441/251‑1 (MQ=255)                                                                                                                                                ggCAAATCGGCACTGGTGATGGGCTGTGTT‑GGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGaa                                                                                             <  2:325351/251‑1 (MQ=255)                                                                                                                                                     aGCGGCGCTGGTGATGGGCTGTGTT‑GGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCGTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGc                                                                                        <  1:184679/251‑1 (MQ=255)                                                                                                                                                        ggCACTTGTGATGGGCTGTGTT‑GGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCAGTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCa                                                                                                                                                       <  2:257833/185‑1 (MQ=255)                                                                                                                                                        ggCACTGGTGATGGGCTGTGTT‑GGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCa                                                                                                                                                       >  1:257833/1‑185 (MQ=255)                                                                                                                                                                                 cggtACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATGTCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGt                                                             <  1:184582/248‑1 (MQ=255)                                                                                                                                                                                  gTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCTTAAAAATGGGTc                                                            <  1:108868/251‑1 (MQ=255)                                                                                                                                                                                     aCTGAAGGTGCGGTCCCTTTCGCCGCGTCCGATGCGCGGCGTGGCATTCCTGCCATCATGGTCGGTTTAGTGTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTTGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTGTGTGGGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCtt                                                          <  2:171722/250‑1 (MQ=255)                                                                                                                                                                                     aCTGAAGGGGCGATTACTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGACGGGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCGGGCGCGTAAAAATGGGTCTTc                                                         >  2:254308/1‑251 (MQ=255)                                                                                                                                                                                       tGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGGCTTCgg                                                       >  1:74040/1‑250 (MQ=255)                                                                                                                                                                                                gATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTATAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGGGGTTGAAGGCAAGCTGGGATATATCGCAGCAGTGGCTGTCGGAGCAGTAGTGACGGCTGGTTGTGTGAACGTGCTGAAAAGTCTGGCGGGGAAAAATgggggttcgaatgaagaa                                               >  1:55192/1‑235 (MQ=255)                                                                                                                                                                                                    ccTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAaag                                           <  1:22260/250‑1 (MQ=255)                                                                                                                                                                                                     cTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGGGCAGTGGTGACGGCTGTTTGTTTGAACGTGCTGAAAAGTCTGGCGCGTAATAATGGGTCTTCGACtgagggaaaaaa                                          >  2:283819/1‑241 (MQ=255)                                                                                                                                                                                                           ccgcTGCCGTTCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAgacgac                                   <  1:140914/251‑1 (MQ=255)                                                                                                                                                                                                                gCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCAGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCtg                                <  1:204631/249‑1 (MQ=255)                                                                                                                                                                                                                  cGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCtggatt                            <  1:2931/251‑1 (MQ=255)                                                                                                                                                                                                                  cGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACGGCGGCGCTTGGCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCtggatt                            <  1:286569/251‑1 (MQ=255)                                                                                                                                                                                                                       cGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCtggatttggat                       <  1:199841/251‑1 (MQ=255)                                                                                                                                                                                                                                    tccgtccATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAaattaat           <  1:353236/246‑1 (MQ=255)                                                                                                                                                                                                                                    gccttccATCATGGTCGTTTTAGTTTGTGGGGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATTCAGGCTGGGGTGGGCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAaattaatt          <  2:99534/250‑1 (MQ=255)                                                                                                                                                                                                                                             catGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAgg  <  1:283819/250‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               ATGCCTTCATGCTGATTTGCGTTGCTCAGGGTGTTTATACCGTGGTGGCCATCGCTGCCGTGGGTATTTGTATCCCGCCGCTGGGGATGGGGCTGGCGACGCTGATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTT‑GGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTCAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGG  >  NC_000913/4143131‑4143615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑