Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,113,851 G→A H208Y (CAT→TAT)  fpr ← flavodoxin/ferredoxin‑NADP(+) reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,113,8510GA94.1% 93.8 / ‑0.7 35H208Y (CAT→TAT) fprflavodoxin/ferredoxin‑NADP(+) reductase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (22/10);  total (23/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.46e-01

TCACTCATTTATCAACCGTTTGATGCCGGCATATCGCGGGATGATCAATAAGATGAGTGCGGATTTGCTCATCGTGCCGTTTATCGATAAGTAACCGCTTACCAGTAATGCTCCGCTGTCATATGGCCCGGTCGGCGACGTAAATGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATGGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTTCCCCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCCGTTTCCCGACTGACCACCGTCTGAATGCGCAGTTTTCCTTCGTAGCGTTTTTCCAGTTCCTGCATCAGTGGCAAATAGCTTAAGTCGGCGGCATAACGTGCGGCGTGCACCAGGACCAGATTTTTGAAGCGATCTAAATCTTTACCTAGTTG  >  NC_000913/4113628‑4114097
                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                      
tcaCTCATTTATCAACCGTTTGATGCCGGCATATCGCGGGATGATCAATAAGATGAGTGCGGATTTGCTCATCGTGCCGTTTATCGATAAGTAACCGCTTACCAGTAATGCTCCGCTGTCATATGGCCCGGTCGGCGACGTAAATGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGcc                                                                                                                                                                                                                             >  1:379749/1‑251 (MQ=255)
    ccATTGATCCACCGTGTCATGCCGGCAGATCGCGGGATGACCAATAAGATGAGTGCTGATTTGGGCGTCGTGCCGTTTAGCCATAAGTAACCGCTTACCAGTAATGCTCCACTGTCATATGGCCCGGTCGGCGACGTAAATGTTTCGTCATCTGCCGGGGCTCGTTCAGCAACTGTTGTGTATCGCGCACCATCTGTTGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCaagc                                                                                                                                                                                                                         <  2:189765/250‑3 (MQ=255)
                cGTTTGATGCCGGCATATCGCGGGATGATCAATAAGATGAGTGCGGATTTGCTCATCGTGCCGTTTATCGATAAGTAACCGCTTACCAGTAATGCTCCGCTGTCATATGGCCCGGTCGGCGACGTAAATGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACCGCATCACATAGCTGGTTTCTTTTTTCATCGGCAGGCCACTCGTGCTTTCCAGt                                                                                                                                                                                                             >  2:181529/1‑251 (MQ=255)
                cGTTTGATGCCGGCATATCGCGGGATGATCAATAAGATGAGTGCGGATTTGCTCATCGTGCCGTTTATCGATAAGTAACCGCTTACCAGTAATGCTCCGCTGTCATATGGCCCGGTCGGCGACGTAAATGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGGGCTTTCCAGt                                                                                                                                                                                                             >  1:103303/1‑251 (MQ=255)
                         ccGGCATATCGCGGGATGATCAATAAGATGAGTGCGGATTTGCTCATCGTGCCGTTTATCGATAAGTAACCGCTTACCAGTAATGCTCCGCTGTCATATGGCCCGGTCGGCGACGTAAATGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGccgggcc                                                                                                                                                                                                                            >  2:136888/1‑221 (MQ=255)
                         ccGGCATATCGCGGGATGATCAATAAGATGAGTGCGGATTTGCTCATCGTGCCGTTTATCGATAAGTAACCGCTTACCAGTAATGCTCCGCTGTCATATGGCCCGGTCGGCGACGTAAATGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCa                                                                                                                                                                                                                            <  1:136888/227‑1 (MQ=255)
                                atCGCGGGATGATCAATAAGATGAGTGCGGATTTGCTCATCGTGCCGTTTATCGATAAGTAACCGCTTACCAGTAATGCTCCGCTGTCATATGGCCCGGTCGGCGACGTAAATGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCCCAGCATCACATAGATGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAATTCCCCACTTTCatttt                                                                                                                                                                                             >  2:266014/1‑247 (MQ=255)
                                      ggATGATCAATAAGATGAGTGCGGATTTGCTCATCGTGCCGTTTATCGATAAGTAACCGCTTACCAGTAATGCTCCGCTGTCATATGGCCCGGTCGGCGACGTAAATGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACCTAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTACCAGTTCCCCCCTTTCAATTAATGCCg                                                                                                                                                                                       >  2:284485/1‑251 (MQ=255)
                                                                           gCCGTTTATCGATAAGTACCCGCTTACCAGGAAGGCTCCGCTGTCATATGGCCCGGTCGGCGACGTAAATTTTTCGTCATCTGCCGGGTCTCTTTCAGCAACGGTTTTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCa                                                                                                                                                                                                                                 <  1:383460/172‑1 (MQ=255)
                                                                           gCCGTTTATAGATAAGTAACCGCTTACCAGTAATGCTCCGCTGTCATATGGACAGGTCGGCGACGTAAATGTTTCGTCCTCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCCCCATCTGCGGATTGCCGCACAGCCTCACCTAGCTGGTTTCTTTATTCATCGGCa                                                                                                                                                                                                                                 >  2:383460/1‑172 (MQ=255)
                                                                            ccGTTTATCGATAAGTAACCGCTTACCAGTAATGCTCCGCTGTCATATGGCCCGGTCGGCGACGTAAATGTTTCGTCATCTGACGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTACAGTTCCCCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCCGTTTCCCg                                                                                                                                                  >  1:153930/1‑250 (MQ=255)
                                                                                ttATCGATAAGTAACCGCTTACCAGTAATGCTCCGCTGTCATATGGCCCGGTCGGCGACGTAAATGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTTCACCCCTTTCAATTAATGCCGGTATCCGTCCGGTGCGCGACCCCGCTGCCGTTTCCCGACTg                                                                                                                                              >  1:167906/1‑250 (MQ=255)
                                                                                    cGATAAGTAACCGCTTACCAGTAAGGCGCCGCGGTCATATGGCCCGGTCGGCGACGTAAATGTTTCGTCAGCTGCCGGGTCCCTTTCAGCAACTGTTGTGGATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTTCCCCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCCGTTTCCCGACTGacca                                                                                                                                          <  2:153930/250‑1 (MQ=255)
                                                                                           tAACCGCTTACCAGTAATGCTCCGCTGTCATATGGCCCGGTCGGCGACGTAAATGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTTCCCCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCCGTTTCCCGACTGACCACCGTCTg                                                                                                                                   >  1:342745/1‑250 (MQ=255)
                                                                                            aaCCGCTTACCAGTAATGCTCCGCTGTCATATGGCACGGTCGGCGACGTAAATGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTTCCCCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCGACCCAGCTGCCGTTTACCGACTGACCACCGTCTGa                                                                                                                                  >  1:345613/1‑250 (MQ=255)
                                                                                                gCTTACCAGTAATGCTCCGCTGTCATATGGCCCGGTCGGCGACGTAAATGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTTCCCCACTTTCACTTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCCGTTTCCCGACTGACCACCGTCTGAATgcg                                                                                                                             >  1:334815/1‑251 (MQ=255)
                                                                                                  ttACCAGTAATGCTCCGCTGTCATATGGCCCGGTCGGCGACGTAAATGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTTCCCCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCCGTTTCCCGACTGACCACCGTCTGAATgcgc                                                                                                                            >  1:104155/1‑250 (MQ=255)
                                                                                                           aTGCTCCGCTGTCATATGGCCCGGTCGGCGACGTAAATGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTTCCCCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCCGTTTCCCGACTGACCACCGTCTGAATGCGCAGTTTTCCtt                                                                                                                  <  1:284485/251‑1 (MQ=255)
                                                                                                                       cATATGGCCCGGTCGGCGACGTAAATGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTTCCCCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCCGTTTCCCGACTGACCACCGTCTGAATGCGCAGTTTTCCTTCGTAGCGttttt                                                                                                      <  1:266014/251‑1 (MQ=255)
                                                                                                                            ggCCAGGTCGGCGACGTAAATGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCCTCGGCAGGCCAATCGCGCTTTCAAGTTCCCCACTTTCAATTAATGCCGGTATCCGTCCGGGGAGCGACCCCGCAGCCGTTTCCCGACTGGCCACCGTCTGAATGCGACGTTTTCCTTCATAGCGTTTTTCCAGt                                                                                                 >  2:314776/1‑251 (MQ=255)
                                                                                                                                      gCGACGTAAATGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATGGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTTCCCCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCGGTTTCCCGACTGACCACCGTCTGAATGCGCAGTTTTCCTTCGTAGCGTTTTTCCAGTTCCTGCATcc                                                                                       >  2:180301/1‑250 (MQ=255)
                                                                                                                                          cGTAAATGTTTCGTCATCTGCCGGGACTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCAAATAGATGGTTTCTTTATTAATCGGCAGGCAAATCGAGATTTCCAGTTCCCCACTTTCAATTAATGCCGGTATCCGTCCGGGGAGCGACCCCGCGGCCGTTTCCCGACTGACCACCGCCTGAATGCGCAGTTTTCCTTCGTAGCGTTTTTCCAGTTCCAGAATCAgagg                                                                                   >  1:319639/1‑248 (MQ=255)
                                                                                                                                           gTAAATGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTACCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTTCCCCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCCGTTTCCCGACTGACCACCGTCTGAATGCGCAGTTTTCCTTCGTCGCGTTTTTCCCGTTCCTGcctccatggc                                                                                  >  2:108396/1‑242 (MQ=255)
                                                                                                                                              aaTGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTTCCCCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCCGTTTCCCGACTGACCACCGTCTGAATGCGCAGTTTTCCTTCGTAGCGTTTTTCCAGTTCCTGCATCAGTGGCaa                                                                                >  1:89337/1‑250 (MQ=255)
                                                                                                                                                 gTTTCGTCATCTGCAGCGTTTCTTTCAGAATCTGTTGTGTAACGCGCACCATCTGTGGATTGCCGCACATCATCACATAGCTGGTTTCTTTATTCTTCGGCAGGCCAATCGTGCTTTCCAGTTCCCCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCCGTTTCCCGACTGACCACCGTCTTAATGCGCAGTTTTCCTTCGTAGCGTTTTTCCAGTTCCTGCATCAGTGGCAAAt                                                                              <  2:251697/249‑1 (MQ=255)
                                                                                                                                                             gCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCCCACAGCCTCACATAGCTGGTTTCTTTATTCATCGGCCGGCCACTCGCGCTTTCCAGTTCCCCACTTTTAATTAATCCCGGTATCCGTCCGGTGAGCGACCCCGCAGCCGCTTCTCGTCGCTCCACCGTCCGAATGCGCGGCTTTCCTTCGGAGCCTTTTTTCCGTTCCTGCATCAGTGGCACACAGTTTAAGTcgccg                                                                >  2:111478/1‑248 (MQ=255)
                                                                                                                                                                         ccaggaaCTGTAGTGTGTCTCGTACTATCGGTGGATTGCCGGACAGCATCACATTGCTGGTTTCTTTATTCATCGGCAGGCCAATCTTGCTTTCCAGTTCCCCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCCGTTTCCCGACTGACCACCGTCTGAATGCGCTGTTTTCCTTCGTAGCGTTTTGTCAGATCCTGCATCAGTGGCAAATAGCTTAAGTCGGCGGCATAACGTgcg                                                    <  1:239799/246‑1 (MQ=255)
                                                                                                                                                                               aCTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTTCCCCACTGTCAATTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCCGTTTCCCGACTGACCACCGTCTGAATGCGCAGTTTTCCTTCGTAGCGTTTTTCCAGTTCCTGCATCAGGGGCAAATAGCTTAAGTCGGCGGCATAACGTGCGGCGTGc                                              <  2:342745/251‑1 (MQ=255)
                                                                                                                                                                                  gttgtGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATGGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTTCCCCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCGGTTTCCCGACTGACCACCGTCTGAATGCGCAGTTTTCCTTCGTAGCGTTTTTCCAGTTCCTGCATCAGTGGCAAATAGCTTAAGTCGGCGGCATAACGTGCGGCGTGCa                                             <  1:180301/249‑1 (MQ=255)
                                                                                                                                                                                  gttgtGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTTCCCCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCCGTTTCCCGACTGACCACCGTCTGAATGCGCAGTTTTCCTTCGTAGCGTTTTTCCAGTTCCTGCATCAGTGGCAAATAGCTTAAGTCGGCGGCATAACGTGCGGCGTGCAc                                            <  2:104155/250‑1 (MQ=255)
                                                                                                                                                                                        tctCGCGCACCATCTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCCTCGGCAGGCCAATCGTGCTTTCCCGTTCCCCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTACCGTTTCCCGACTGACCACCGTCTGAATGCGCAGTTTTCCTTCGTAGCTTTTTTCCAGTTCCTGAATCAGTGGCCAATAGCTTAAGTCGGCGGCATAACGTGCGGCGGGCACCAggccc                                     >  2:79265/3‑248 (MQ=255)
                                                                                                                                                                                                       ttgGATTGCCGCACAGCATCACATAGCTGTTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTTCCCCTCTTTCAATTAAGGCCTGTATCCGGCCGGTGAGCGACCCCGCTGCCGTTTCCCGACTGACCACCGTCTGAATGCGCAGTTTTCCTTCGTAGCGTTTTTCCAGTTCCTGCATCAGTGGCAAATAGCTTAATTCGGCGGCATAACGTGCGGCGTGCACCAGGACCAGATTTTTGAAg                         <  2:382687/247‑1 (MQ=255)
                                                                                                                                                                                                                    caGCAACACATAGCAGGCTTGTTTATTCATCGGCAGGCCAATCGATCTTTCCAGTTCCCCACTTTCACTTAATGCCGGTATCCGTCCAGTGAGCGACCCCGCTGCCGTTTCCCAACTGCCCACCGCCTGAACGCGCAGTTTTCCTTCGTAGCGTTTTTCCAGTTCCTGCATCAGTGGAAAAAAGCTTAAACCGGCGGCATAACGTGCGGCGTGCACCCGGACCAAATTTTTGAAGCGGTATAAATCttacc         >  2:233283/1‑248 (MQ=255)
                                                                                                                                                                                                                          cacaTAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTTCCCCACTTTCTATTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCCGTTTCCCGACTGACCACCGTCTGAATGCGCAGTTTTCCTTCGTAGCGTTTTTCCCGTTCCTGCTTCAGTGGCAAATAGCTTAAGTCGGCGGCCTAACGTGCGGCGTGCACCAGGACCAGTTTTTTGAAGCGTTCTAAATCTTTACCTAGtt   >  2:83265/1‑251 (MQ=255)
                                                                                                                                                                                                                           acaTAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAATTCCCAACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCGAACCCGCTGCCGTCTACAGACTGACCACCGTCTGAATGCGCGGATTTCATTCGTAGCGTTTTTCCAGTTCCTTCCTCAGGGGGAAACAGCTTAAATCGGCGGCCTAACGTGCGGCGGAAACCAGGACGCGATTTTTGCAGCGATCTCATTCTTTACCtcattg  >  2:99165/1‑246 (MQ=255)
                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                      
TCACTCATTTATCAACCGTTTGATGCCGGCATATCGCGGGATGATCAATAAGATGAGTGCGGATTTGCTCATCGTGCCGTTTATCGATAAGTAACCGCTTACCAGTAATGCTCCGCTGTCATATGGCCCGGTCGGCGACGTAAATGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATGGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTTCCCCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCCGTTTCCCGACTGACCACCGTCTGAATGCGCAGTTTTCCTTCGTAGCGTTTTTCCAGTTCCTGCATCAGTGGCAAATAGCTTAAGTCGGCGGCATAACGTGCGGCGTGCACCAGGACCAGATTTTTGAAGCGATCTAAATCTTTACCTAGTTG  >  NC_000913/4113628‑4114097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: