Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	30,237	C→T	A196V (GCT→GTT)	carA →	carbamoyl‑phosphate synthetase small subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	30,237	0	C	T	98.3%	177.4 / ‑4.2	58	A196V (GCT→GTT)	carA	carbamoyl‑phosphate synthetase small subunit
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (30/27);  total (30/28)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.85e-01

ACCCGTAAGCTGACGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGCATGGATCTGGCAAAAGAAGTGACCACCGCAGAAGCCTATAGCTGGACACAAGGGAGCTGGACGTTGACCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGCTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGCCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCGGTATTCGGCATCTGTCTCGGTCATCAGCTGCTGGCGCTGG  >  NC_000913/29993‑30482                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      aCCCGTAAGCTGACGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGCATGGATCTGGCAAAAGAAGTGACCACCGCAGAAGCCTATAGCTGGACACAAGGGAGCTGGACGTTGACCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGa                                                                                                                                                                                                                                                 <  2:87375/251‑1 (MQ=255)   ccGTAAGCTGACGCGGTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCGGAATGGCATGGATCTGGCAAAAGAAGTGACCACCGCAGAAGCCTATAGCTGGACACAAGGGAGCTGGACGTTGACCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGAtt                                                                                                                                                                                                                                               <  2:377794/251‑1 (MQ=255)       acgCTGACGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGCATGGATCTGGCAAAAGAAGTGACCACCGCAGAAGCCTATAGCTGGACACAAGGGAGCTGGACGTTGCCCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCAAGTCGTGGTTTATGATTTTgg                                                                                                                                                                                                                                           >  2:296844/3‑251 (MQ=255)            gACGCGTTTACTGCGCGAGAAAGGAGCACAGGAGGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCGGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGCATGGATCTTGCAAAAGAAGTTACCACCTCAGAAGCCTATAGCTGGACACAAGGTAGCTGGACGTTGACCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGAtttttttgcca                                                                                                                                                                                                                                      <  2:364707/251‑8 (MQ=255)              cgcgTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGCATGGATCTGGCAAAAGAAGTGACCACCGCAGAAGCCTATAGCTGGACACAAGGGAGCTGGACGTTGACCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGTGCCAAg                                                                                                                                                                                                                                    <  1:294948/251‑1 (MQ=255)                cgTTTACTGCGCGAGAAAGGCGCACAGAATTGCTGCATTATCGCGGGCGATAACCCGGATGCGGTGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGCATGGAGCTGGCAAAAGAAGTGACCACCGCAGAAGCCTATAGCTGGACACAAGGGAGCTGGACGTTGTCCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGTGCCAAgcg                                                                                                                                                                                                                                  <  1:285780/251‑1 (MQ=255)                    tACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGCATGGATCTGGCAAAAGAAGTGACCACCGCAGAAGCCTATAGCTGGACACAAGGGAGCTGGACGTTGACCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGTGCCAAGCGCaa                                                                                                                                                                                                                               <  1:141158/250‑1 (MQ=255)                           cgAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGCATGGATCTGGCAAAAGAAGTGACCACCGCAGAAGCCTATAGCTGGACACAAGGGAGCTGGACGTTGACCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGTGCCAAg                                                                                                                                                                                                                                    <  2:158524/238‑1 (MQ=255)                           cgAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGCATGGATCTGGCAAAAGAAGTGACCACCGCAGAAGCCTATAGCTGGACACAAGGGAGCTGGACGTTGACCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGTGCCAAg                                                                                                                                                                                                                                    >  1:158524/1‑238 (MQ=255)                                aaGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGCATGGATCTGGCAAAAGAAGTGACCACCGCAGAAGCCTATAGCTGGACACAAGGGAGCTGGACGTTGACCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATg                                                                                                                                                                                                                  <  2:185379/251‑1 (MQ=255)                                  ggCGCACAGAATGGCTGCTTTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGCATGGATCTGGCAAAAGAAGTGACCACCGCAGAAGCCTATAGCTGGACACAAGGGAGCTGGACGTTGACCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATGCt                                                                                                                                                                                                                <  1:85245/251‑1 (MQ=255)                                                   aaTTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGCAGGGATCTGGCAAAAGAAGTGACCACCGCAGAAGCCTATAGCTGGACACAAGGGAGCTGGACGTTGACCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTc                                                                                                                                                                                               <  2:31647/250‑1 (MQ=255)                                                   aaTTACCGCGGGCGATAACCCGGAGGCGGCGCGGGCGTAAGAAAAAGCCCGCGCGTTCCCAGGCCGGAATGGCAGGGATCTGGCAAAAGAAGTGACCACCGCAGAAGCCTATAGCTGGACACAAGGGAGCTGGACGTTGACCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGGGGTTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTc                                                                                                                                                                                               <  2:39777/250‑1 (MQ=255)                                                                gATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGCATGGATCTGGCAAAAGAAGTGACCACCGCAGAAGCCTATAGCTGGACACAAGGGAGCTGGACGTTGCCCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGTGCCAAGCGCACCATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGt                                                                                                                                                                                  >  1:393232/1‑251 (MQ=255)                                                                gATAACCCGAATGAGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGCATGGATCTGGCAAAAGAAGTGACCACCGCAGCAGCCTATAGCTGGACACAAGGGAGCTGGACGTTGACCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCCCGTCGTGGTTTTTGATTTTGGTGCCAAGCGCAACATCCTGCGGCCGGTGGTGGATAGAGGCTGtagaattaccaactt                                                                                                                                                                                  >  2:1930/1‑237 (MQ=255)                                                                     cccGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGCAGGGATCTGGCAAAAGAAGTGACCACCGCAGAAGCCTATAGCTGGACACAAGGGAGCTGGACGTTGACCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCgg                                                                                                                                                                             <  2:312859/251‑1 (MQ=255)                                                                       cGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGCATGGATCTGGCAAAAGAAGTGACCACCGCAGAAGCCTATAGCTGGACACAAGGGAGCTGGACGTTGACCGGTGGCCTGCCCGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGGGCCAAGCGCAACATCCTGCGGATGCTGGCGGATAGAGGCTGGCGCCTGACCATCGTTCCGgcg                                                                                                                                                                           >  2:251127/1‑251 (MQ=255)                                                                           tGCGGCGCTGGCGTTAGAAAAAACCCGCGCGTTCCCAGGTCTGAATGGCATGGCTCTGGCAAAAGAAATGACCACCGCAGAAGCCTATAGCTGGACACAAGGGAGCTGGACGTTGACCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGGGCCAAGAGCACCATCCTGCGGATGCTGGTTGATAGAGGCTGTCGCCTGACCATCGTTCCGgcgcca                                                                                                                                                                        >  2:375394/1‑248 (MQ=255)                                                                             gggcgCTGGCGTTAGAAAAAGCCCGCGCGTTACCAGGTTTGAATGGCATGGATCTGGCAAAAGAAGTGACCACCGCAGAAGCCTATAGCTGGACACAAGGGAGCTGGACGTTGACCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACt                                                                                                                                                                     <  2:79506/250‑1 (MQ=255)                                                                                   tGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGCATGGATCTGGCAAAAGAAGTGACCACCGCAGAAGCCTATAGCTGGACACAAGGGAGCTGGACGTTGACCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGTGCCAAGCGCAACATCCt                                                                                                                                                                                                                         <  1:273673/193‑1 (MQ=255)                                                                                   tGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGCATGGATCTGGCAAAAGAAGTGACCACCGCAGAAGCCTATAGCTGGACACAAGGGAGCTGGACGTTGACCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGTGCCAAGCGCAACATCCt                                                                                                                                                                                                                         >  2:273673/1‑193 (MQ=255)                                                                                        ttAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGCATGGATCTGGCAAAAGAAGTGACCACCGCAGAAGCCTATAGCTGGACACAAGGGAGCTGGACGTTGACCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCACCTGACCATAGTTCCGGCGCCAACtttgcaggaga                                                                                                                                                           >  2:332618/1‑241 (MQ=255)                                                                                             aaaaGCCCGCGCGTTCCCAGGTCTGAATGGCATGGATCTGGCAAAAGAAGTGACCACCGCAGAAGCCTATAGCTGGACACAAGGGAGCTGGACGTTGACCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATGCTGGTGGCTAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACTTCTGCGGAAGATGTGc                                                                                                                                                     >  1:82298/1‑251 (MQ=255)                                                                                                   ccGCGCGTTCCCCGGTCTGAATGGCATGGATCTGGCAAAAGAAGTGACCACATAAGAAGCCTATAGCTGGACACAGGGGAGCTGGACGTTGACCGGAGTCCTGACAGTAGAAAAAGAAGTAGACGAGCTGCCGTTCCACGTCGTGATTTATGATTTTGGTGCCAGGCGCCGCATCCTGCGGGTGCTGGTGGATAGAGGCTATCGCCTGACCATCTGTCCGGCGCAACCTTCTGCGCGCGATGTGCTGaaa                                                                                                                                                >  1:403755/1‑250 (MQ=255)                                                                                                    cgcgcgTTCCCAGGTCTGAATGGCATGGATCTGGCAAAAGAAGTGACCACCGCAGAAGCCTATAGCTGGACACAAGGGAGCTGGACGTTGACCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACTTCTGCGGAAGATGTGCTGAAAAt                                                                                                                                              <  1:64470/251‑1 (MQ=255)                                                                                                              cAGGTCTGAATGGCATGGATCTGGCAAAAGAAGTGACCACCGCAGAAGCCTATAGCTGGACACAAGGGAGCTGGACGTTGACCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGTGCCACGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCCTCGTTCCGGCGCCACATTCTGCGGAAGATGGGCTGAAAATGAATCCAGAc                                                                                                                                    >  2:353744/1‑251 (MQ=255)                                                                                                                     gAATGGCATGGATCTGGCAAAAGAAGTGACCACCGCAGAAGCCTATAGCTGGACACAAGGGAGCTGGACGTTGACCGGTGGCCGGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATc                                                                                                                              <  1:364517/250‑1 (MQ=255)                                                                                                                         ggCATGGACCTGGCAAAAGAAGTGACCACCGCAGAAGCCTATAGCTGGACACAAGGGAGCTGGACGTTGACCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTcc                                                                                                                          <  1:236238/250‑1 (MQ=255)                                                                                                                           cATGGATCTGGCAAAAGAAGTGACCACCGCAGAAGCCTATAGCTGGACACAAGGGAGCTGGACGTTGACCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACTTCTGCGGAGGATGTGCTGAAAATGAATCCAGACGGCATCTTCctct                                                                                                                       <  1:411075/251‑1 (MQ=255)                                                                                                                             tGGATCTGGCAAAAGAAGTGACCACCGCAGAAGCCTATAGCTGGACACAAGGGAGCTGGACGTTGACCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTcc                                                                                                                     >  2:303575/1‑251 (MQ=255)                                                                                                                                    ggCAAAAGAAGTGACCACCGCAGAAGCCTATAGCTGGACACAAGGGAGCTGGACGTTGACCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTc                                                                                                              >  1:323490/1‑251 (MQ=255)                                                                                                                                      cAAAAGAAGAGACCACAGCAGAAGCCTATAGCTCGACACAAGGGAGCAGGACGTTGACCGGTGGCCTGCCAGACGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCCTCGTTCCGGCGCAAACTTCTGCGGCAGATGTGCTGTACATAAATCCAAACGGCATCTTCCTCTCCAACGGTCCt                                                                                                            >  2:233289/1‑251 (MQ=255)                                                                                                                                       aaaaGAAGTGACCACCGCAGAAGCCTATAGCTGGACACAAGGGAGCTGGACGTTGACCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCCTCCTCCTCTCCCACGGTCCTg                                                                                                           >  2:385470/1‑251 (MQ=255)                                                                                                                                        aaaGAAGTGACCACCGCAGAAGCCTATAGCTGGACACAAGGGAGCTGGACGTTGACCGGTGGCCTGCCAGAAGCGAAACAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGGTCCGGCGCAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCCGACGGCCTCTTCCTCTCCAACCGCCCTgg                                                                                                          >  2:136594/1‑251 (MQ=255)                                                                                                                                         aagaagTGACCACCGCAGAAGCCTATAGCTGGACACAAGGGAGCTGGACGTTGACCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACTTCTGCGGCAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGc                                                                                                         >  1:115323/1‑251 (MQ=255)                                                                                                                                                gACCACCGCAGAAGCCTATAGCTGGACACAAGGGACCTGGACGTTGACCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCCAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCgg                                                                                                  >  1:113406/1‑251 (MQ=255)                                                                                                                                                     ccGCAGAAGCCTATAGCTGGACACAAGGGAGCTGGACGTTGACCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGCCCCg                                                                                             >  1:259488/1‑251 (MQ=255)                                                                                                                                                                 ataGCTGGACACAAGGGAGCTGGACGTTGACCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGCTTATGATTTTGGGGCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGCCCCGTGCGATTACGcc                                                                                 <  2:100069/251‑1 (MQ=255)                                                                                                                                                                       ggACACAAGGGAGCTGGACGTTGACCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGCCCATCGTTCCGGCGCAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCCACGGTCCTGGCGACCAGGCCCCGTGCGGTTTCGCCATTAcc                                                                           >  2:355936/1‑251 (MQ=255)                                                                                                                                                                             aaGGGAGCTGGACGTTGACCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGCCCCGTGCGATTACGCCATTACCGCCATc                                                                     <  1:385470/251‑1 (MQ=255)                                                                                                                                                                             aaGGGAGCTGGACGTTGACCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGCCCCGTGCGATTACGCCATTACCGCCATc                                                                     <  1:296844/251‑1 (MQ=255)                                                                                                                                                                               gggAGGTGGACGTTGACCGGGGGCCTGCCAGAAGAGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGGGGTTGATGATTGTGGTGCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGCCCCGTGCGATTACGCCATTACCGCCATCCa                                                                   <  2:82298/251‑1 (MQ=255)                                                                                                                                                                                ggAGCTGGACGTTGACCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGTGCCAAGCGAAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACTTCTGCGGAGGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGCCCCGGGCGATTACGCCCTTACCCCCATcc                                                                    >  2:416151/1‑249 (MQ=255)                                                                                                                                                                                      ggACGTTGACCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACTTCTGc                                                                                                                                                                >  2:83150/1‑151 (MQ=255)                                                                                                                                                                                      ggACGTTGACCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACTTCTGc                                                                                                                                                                <  1:83150/151‑1 (MQ=255)                                                                                                                                                                                         cGTTTACCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGTGCCAAGCTCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGCCCCGTGCGATTACGCCATTACCGCCAGCCAGAAATTCCTc                                                         <  1:293766/251‑1 (MQ=255)                                                                                                                                                                                          gTTGACCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCAt                                                                                                                               <  2:227489/180‑1 (MQ=255)                                                                                                                                                                                          gTTGACCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCAt                                                                                                                               >  1:227489/1‑180 (MQ=255)                                                                                                                                                                                          gTTGACCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGCCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCg                                                        >  1:17439/1‑251 (MQ=255)                                                                                                                                                                                                    ggCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGCCCCGTGCGAtt                                                                                      >  1:228829/1‑211 (MQ=255)                                                                                                                                                                                                    ggCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGCCCCGTGCGAtt                                                                                      <  2:228829/211‑1 (MQ=255)                                                                                                                                                                                                        ggCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCAACGTCGTGGTTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCACCGTTCCGGCGCAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGCCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCg                                          <  2:160600/250‑1 (MQ=255)                                                                                                                                                                                                         gCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGCCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCgg                                         >  1:3158/1‑251 (MQ=255)                                                                                                                                                                                                         gCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGACCTGGCGACCCGGCCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCgg                                         <  2:146146/251‑1 (MQ=255)                                                                                                                                                                                                             gAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGCCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCGGTAtt                                     >  1:264062/1‑251 (MQ=255)                                                                                                                                                                                                                         gaagaCGAGCTGCCGTTCCACGTCGTGGTTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGCCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAACCCGATATTCCGGTATTCGGCATCTGtcc                         >  2:346155/1‑250 (MQ=255)                                                                                                                                                                                                                                       gTTCCACGTCGTGGTTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGCCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCGGTATTCGGCATCTGTCTCGGTCATCAgctgc           >  1:114904/1‑251 (MQ=255)                                                                                                                                                                                                                                                cgtGGTTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCCAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCATCGGTCCTGGCGACCCGGCCCCGTGCGATTCCGCCATAACAGCCATCCAGAAATTCCTCGAAACCGATATTCCGGTATTCGGCATCTGTCTCGGTCATCAGCTgctggggctgg  >  1:270022/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      ACCCGTAAGCTGACGCGTTTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCGGCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGCATGGATCTGGCAAAAGAAGTGACCACCGCAGAAGCCTATAGCTGGACACAAGGGAGCTGGACGTTGACCGGTGGCCTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGCTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAACGGTCCTGGCGACCCGGCCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAAACCGATATTCCGGTATTCGGCATCTGTCTCGGTCATCAGCTGCTGGCGCTGG  >  NC_000913/29993‑30482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑