Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 1,907,451 IS2 (+) +5 bp coding (137‑141/144 nt) yobF ← DUF2527 domain‑containing protein YobF

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 1651548NA (NA)32 (0.820) 32/480 0.3 96.9% noncoding (706/706 nt) IS2 repeat region
?NC_000913 1907451 = 1 (0.030)coding (141/144 nt) yobF DUF2527 domain‑containing protein YobF
* ? NC_000913 = 19074551 (0.030)28 (0.720) 25/480 0.5 96.5% coding (137/144 nt) yobF DUF2527 domain‑containing protein YobF
?NC_000913 = 2070271 NA (NA)noncoding (1/1331 nt) IS2 repeat region

AGAAATAACTGGCTGGCTGCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTGTACGGTTCACAGGCAATTGGCGGTATTGTTAACTTCATCACCAAAAAGGGTGAATCTCCGGACTCCCTATATCACTTAAATT  >  NC_000913/2070038‑2070502
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                       
agaAATCACTGGCTGGCTGATACACCATGTTGCCGGGCAACGCGGAAAACCGTCATCCCCGATTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGAGGTACGCCGTCTGCGTTTCTCCGACCCTAAGACATCCATCATCTGTTCTCCAATGCCTAGTATAAAAACTAATATTAAGACTATCACTTATTTAAGTGATATTGTTTGTCTGGAGATTAAGAGGGACAGACTAGTGACTAAGCAtcctcc                                                                                                                                                                                                                        >  2:48819/1‑251 (MQ=11)
                   ctACACCATGTTGCCGGGCAACGAGGGCGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCTATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTATGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTg                                                                                                                                                                                                     >  1:97480/1‑251 (MQ=255)
                   ctACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGTGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTg                                                                                                                                                                                                     >  2:135860/1‑251 (MQ=255)
                                   ggCAACTAGGGAGACCGTCATCCCCGGTTCAAAGCTCGTCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAAGCATCTGTTCTCCAATGACTAGTCTAAAATCTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGGTTTAATTCTCATTGATGGTCAGGAGGTaa                                                                                                                                                                                      <  2:330269/250‑1 (MQ=255)
                                                       tCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGAt                                                                                                                                                                  >  2:102611/1‑250 (MQ=255)
                                                                  aaGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGGGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGtgtg                                                                                                                                                      >  1:35975/1‑251 (MQ=255)
                                                                                aaTTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGaa                                                                                                                                           >  2:316249/1‑247 (MQ=255)
                                                                                                        aCGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTgg                                                                                                                >  1:16688/1‑250 (MQ=255)
                                                                                                             gTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAAGTAGTATTAAGACTATCACTTATTTAAGGGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGAGAATGATGGTGTGGGACTGTTGATAGATGAGTCTgc                                                                                                                               <  2:152675/231‑1 (MQ=255)
                                                                                                             gTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCGCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGAGTGGGACTGTTGATAGATGGGTCTgcagatcggacaagcgcacgtc                                                                                                           >  1:152675/1‑231 (MQ=21)
                                                                                                                         ccgccccTAAGACATCAATCAGCTTCTCTCCAATGGCTAGTCGAAAAACTAGTATTAAGACTATCACTTATTTGATGGATATTGGTTGGCTGTAGATTCAGGGTGCCAGTCTAGTTTCGAAGCATTCGCCCGTGTTTTAATGCTCATGGTTGTTCAGGAGGTAAGTTATCAGCGCGCCGGAGATAATTATGTTGTGGGAGTGGTGATAGATGAGTCGGCGCTGGAGCGTGTTGAGGTTGTGAAAGGttc                                                                                                 <  2:205291/245‑3 (MQ=16)
                                                                                                                           ggCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCatat                                                                                             >  1:100945/1‑251 (MQ=255)
                                                                                                                                 aaGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGt                                                                                        >  1:272659/1‑250 (MQ=255)
                                                                                                                                    acaacaatcaTCTGTTCTCCAATGACTAGTCTAACAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAACATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGAGCCGGAGATAAATATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTg                                                                                    >  1:135393/5‑251 (MQ=255)
                                                                                                                                            catcTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTGTACGGTTc                                                                            >  2:70829/1‑251 (MQ=255)
                                                                                                                                                         aTGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGCTGGTCAGGAGGTaa                                                                                                                                                                                      >  2:179347/1‑132 (MQ=255)
                                                                                                                                                         aTGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTaa                                                                                                                                                                                      <  1:179347/132‑1 (MQ=255)
                                                                                                                                                                             tATTCAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGATATTCAGGAGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGCTAATTATGGTGTTGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTCCTGTACGGTTCACAGGCAATTGGCGGGATTGTTAACTtcatacc                                           >  2:63848/1‑248 (MQ=255)
                                                                                                                                                                                   gACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCCTTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAAtt                                                                                                                                                              >  2:53048/1‑130 (MQ=255)
                                                                                                                                                                                   gACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAAtt                                                                                                                                                              <  1:53048/130‑1 (MQ=255)
                                                                                                                                                                                       atcccTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGTCTGTCTAGTGGCGAAGCATCCGCCCGTGTTTTAATTCTCAGTGATGGTCAGGAGGTAACTTAGCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTGTACGGTTCACAGGCAATTGGCGGTATTGCTAACTTCATCACCaaaaac                                    <  2:307063/244‑2 (MQ=255)
                                                                                                                                                                                       aTCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATTGTCAGGAGGTAACTGATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTGTACGGTTCACAGGCAATTGGCGGTATTGTTAACTTCATCACCAAAAAGGGt                                 <  1:62755/251‑1 (MQ=255)
                                                                                                                                                                                                   aGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTGTACGGTTCACAGGCAATTGGCGGTATTGTTAACTTCATCACCAAAAAGGGGGAATCTCCGGa                      >  1:277850/1‑250 (MQ=255)
                                                                                                                                                                                                     tGATATTGGCTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGAATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGAAAATTATCCGCGCGCCGGAGATAATTATGGTGTGGGTCTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTTGTGAAAGGTCAATATTCCGTACTGTACGGTTCACAGGCAATTGGCGGTATTGTTAACTTCATCACCAAAAAGGGTGAATCTCCgggcac                   >  2:401437/1‑247 (MQ=255)
                                                                                                                                                                                                      gATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTGTACGGTTCACAGGCAATTGGCGGTATTGTTAACTTCATCACCAAAAAGGGTGAATCTCCGGACTcc                  <  1:102611/251‑1 (MQ=255)
                                                                                                                                                                                                           ttgTTGGCTGGAGAGTCAGGGGGCGAGTCTAGTGGCGAAGCATTCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGGACTGTACGTTTCACAGGCAATTGGCGGTATTGTTAACTTCGTTTCCAAAAATTGTGAATCTCCGGACTCCCtac              <  2:59618/248‑2 (MQ=255)
                                                                                                                                                                                                                gTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTGTACGGTTCACAGGCAATTGGCGGTATTGTTAACt                                                  <  2:129159/209‑1 (MQ=255)
                                                                                                                                                                                                                gTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTGTACGGTTCACAGGCAATTGGCGGTATTGTTAACt                                                  >  1:129159/1‑209 (MQ=255)
                                                                                                                                                                                                                 gcTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATGCCGTACTGTACGGTTCACAGGCAATTGGCGGTATTGTTAACTTCATCACCAAAAAGGATGAATCCCCGGACTCCCTATATCACtt       <  1:63848/250‑1 (MQ=255)
                                                                                                                                                                                                                      ggaTTCAGGGGGCCAGTCTAGTGGCGAAGCAGCCCCCCGTGTTTTAATGCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTTCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTGTACGGTTCACAGGCAATTGGCGGTATTGTTAACTTCATCACCAAAAAGGGTGAATCTCCGGACTCCCTATATCACTTAAAtt  <  2:51225/250‑1 (MQ=255)
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                       
AGAAATAACTGGCTGGCTGCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTGTACGGTTCACAGGCAATTGGCGGTATTGTTAACTTCATCACCAAAAAGGGTGAATCTCCGGACTCCCTATATCACTTAAATT  >  NC_000913/2070038‑2070502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.