| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| JC | NC_000913 | 1,333,262 | Δ3 bp | coding (2215‑2217/2598 nt) | topA → | DNA topoisomerase 1 |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913 | = 1333261 | 2 (0.050) | 20 (0.510) | 20/482 | 0.9 | 90.9% | coding (2214/2598 nt) | topA | DNA topoisomerase 1 |
| ? | NC_000913 | 1333265 = | 2 (0.050) | coding (2218/2598 nt) | topA | DNA topoisomerase 1 | |||||
GCAAAACGTCGTTGCCCGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAG‑AGTGTAAAAACACACGTAAGATTTTACGTAACGGCGAAGTGGCACCAC‑CGAAAGAAGATCCGGTGCCATTACCTGAGCTGCCGTGCGAAAAATCAGATGCTTATTTCGTGCTGCGTGACGGTGCTGCCGGTGTGTTCCTGGCTGCCAACACTTTCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCG > NC_000913/1333019‑1333498 | gcAAAACGTCGTTGCCCGAAATGCGGCACGTCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAG‑AGTGTAAAAACcgtaaga > 1:399875‑M1/1‑243 (MQ=255) gACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAG‑AGTGTAAAAACcgtaagattttacgtaacggcgaagtggcaccaccgaaagaaga < 1:327442‑M1/251‑45 (MQ=255) tATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAG‑AGTGTAAAAACcgtaagattttacgtaacggcgaagtggcaccaccgacagaagatccggg > 1:198714‑M1/1‑201 (MQ=255) atcataaacgtatGTTGCATTACAGTGGTAATAACCTAATCTTCGACGGTTACGAGAACGAAGATGGCGAATCCCGCATTAAAGGTTATGACGTCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATTCACCTGAAAATGGGGCGTTGCGTTAAATACATGGCCTTCACCACCGAAG‑AGTGTAAAAACcgtaagattttacgtaccggcgaagtggcaccaccgaaagaagatccggtgccattacct < 2:327922‑M1/246‑61 (MQ=255) ccGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGACG‑AGTGTAAAAACcgtaagattttacgtaccggcgaagtggcaccaccgaaagacgatacggtgccattacctg > 2:396814‑M1/1‑189 (MQ=255) aaagtaaGTGGCATGTCGGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAAGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAG‑AGTGTAAAAACcgtaagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctg < 2:398762‑M1/248‑67 (MQ=255) cGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAG‑AGTGTAAAAACcgtaagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgcc < 2:266546‑M1/251‑69 (MQ=255) gtgGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTGGAGGGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAG‑AGTGTAAAAACcgtaagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagat < 2:399875‑M1/251‑85 (MQ=255) aaCCTGCGACGGTTACGAGATCGAAGAGGTCGACTTCCGCATTAAAGGTTATGACGGCCCGGTCGTTGAGAGAGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAG‑AGTGTAAAAACcgtaagattttacgtaacggcgcagtggccccaccgaaagaagatccagtgccattacccgcgccaccgtgcgaaaaatcagatgtctatttctggc > 2:298198‑M1/1‑154 (MQ=255) ggcGAAGGTGACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAG‑AGTGTAAAAACcgtaagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcg < 2:198714‑M1/250‑102 (MQ=255) tACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAG‑AGTGTAAAAACcgtaagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtg < 1:251707‑M1/250‑110 (MQ=255) ggCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAACTACATGGCCTGCACCAACGAAG‑AGTGTAAAAACcgtaagattttacgtcacggcgaagttgcaccaccgaaagaagatccggtgccattacctgagctgccgagcgacaaatcagatgcttatttcgggctgcgtgccgatgctggcggggggatact > 2:388334‑M1/1‑126 (MQ=255) aTTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAG‑AGTGTAAAAACcgtaagagtttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaagcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctg < 1:382377‑M1/251‑131 (MQ=255) cctgtCGTTGAGTTTTAAAAAAGGGGGTCTGAAAGGCACCTGAAAAGGGGGCATTTCGGGAAAAACATGGCCTGCACCAACGAAG‑AGGGTAAAAAC‑‑‑CGTAAGATTTTACGTAAGGGCGAAGGGGCACCAC‑CGAAAGAAGATCCGGTGCCATTGCCTGAGGTGCCGTGCGAAAAATCAGATGCTTATTTCGTGCTGCGTGACGGTGCTGCCGGTGTGTTCATGGCTGCCAACTCGTTCCCGAAATCGCGTGa < 2:313974/247‑1 (MQ=255) tgtgAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGAGTTTCGGTAAATACATGGCCTGCACCAACGAAG‑AGTGTAAAAACcgtaagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttcccgaaatcgcgtgaaacgcgtgcgcc > 1:159250‑M1/1‑84 (MQ=255) gCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAG‑AGTGTAAAAACcgtaagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttcccgaaatcgcgtgaaacgcgtgcgccactggtggaagag < 1:323235‑M1/251‑181 (MQ=255) gAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAG‑AGTGTAAAAACcgtaagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttcccgaaatcgcgtgaaacgcgtgcgccactggtggaagagcttt < 1:396814‑M1/250‑185 (MQ=255) cACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAG‑AGTGTAAAAACcgtaagattttacgtaccggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgcggccggtgtgttcctggctgccaacactttcccgaaatcgcgtgaaacgcgtgcgccactggtggaagagctttatcgctt < 1:207405‑M1/251‑192 (MQ=255) aTGGGGCGTTTCGGTAAACACATGGCCTGCACCAACGAAGAAGT‑TAAAAACcgtaacatcttactttacggcgaagtggccccaccaaaagaagatcctgtgccattacctgagcggccgtgcgaaaaatcagatgcttattgcgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttcccgaaatcgcgtgaaacgcgtgcgccactggtggaagatctttatctcttccgcgaccg < 2:156521‑M1/251‑201 (MQ=255) tCGGTAAATACATGGCCTGCACCAACGAAG‑AGTGTAAAAACcgtaagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttcccgaaatcgcgtgaaacgcgtgcgctactggtggaagagctttatcgcttccgcgaccgtctgccgga < 1:15929‑M1/250‑210 (MQ=255) aCATGGCATGCAACAAAGAAG‑AGTGTAAAAACA‑‑‑GTAAGATTTTAAGTAACGGCGAAGTGGCAC‑ACACGAAAGAAGATCCGGTGCCATTACCGGAGCTGCCGTGCGAGAAATCAGATGCTTATTTCGTGCTGCGTGACGGTGCTGCCGGTGAGTTACTGGCTGCCAACTCTTTCACGAAATCGCGTGACACGCGGGCGCCACTGGTGGAAGAGCTTTCTCGCCTCCGCGACTGTCGGCCGGAAAAAcagagg > 2:82220/1‑246 (MQ=255) g‑agTGTAAAACCcgtaagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttcccgaaatcgcgtgaaacgcgtgcgccactggtggaagagctttatcgcttccgcgaccgtctgccggaaaaactgcgttatctggccgatgcgccac > 2:50691‑M1/1‑12 (MQ=255) | GCAAAACGTCGTTGCCCGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAG‑AGTGTAAAAACACACGTAAGATTTTACGTAACGGCGAAGTGGCACCAC‑CGAAAGAAGATCCGGTGCCATTACCTGAGCTGCCGTGCGAAAAATCAGATGCTTATTTCGTGCTGCGTGACGGTGCTGCCGGTGTGTTCCTGGCTGCCAACACTTTCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCG > NC_000913/1333019‑1333498 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |