Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,745,089	0	A	C	30.8%	48.4 / 11.4	41	V84G (GTG→GGG)	trmD	tRNA m(1)G37 methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (24/3);  new base C (1/11);  total (25/16)
Fisher's exact test for biased strand distribution p-value = 2.35e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTC‑GTAGCGACCGCACACCAGAAT‑CAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGTAATTGCGCGGAACATTTCAGGAAACAGGCTAATTATGCCAATCC  >  NC_000913/2744865‑2745335                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       accccGGAAAAAACCGGGAAACGGAATCAATCATCCGCATTGCTGGTAACGCACCCCCATTGAGAACGAAATCGCCGATTGACTTTTCCTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATCCCTGC‑GAAGCGACCGCACCCGAGAGT‑CAATTTTTGGTTCGTTGCGAGTTCGTTGACGCCTTCTGGAGCAAGCGTGGGTCCGTGGTTTGACAGAAAGATCTCCTTTGCGCCtccccttggcgcggct                                                                                                                                                                                                                              <  2:431482/249‑16 (MQ=255)   cccGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTC‑GTAGCGACCGCACACCAGAAT‑CAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCTCCGCGGCttt                                                                                                                                                                                                                            >  1:179709/1‑251 (MQ=255)   accGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACAACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCACTTTCGGTTTAGATCACGCGCTCATCTATACCTTC‑GTAGCGACCGCCCACCAGAAT‑CAATTTTTGATTAGTTGCCAGTTCGCTGACGCACGCTTGATCAAGCTTGCGTCCCTGTGGTGTCCGATAAATCACCTTTGAGCCTTCACCCGCCGCGGCttt                                                                                                                                                                                                                            >  1:300667/2‑251 (MQ=255)                gggAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTC‑GTAGCGACCGCACACCAGAAT‑CAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATg                                                                                                                                                                                                               <  1:184526/251‑1 (MQ=255)                                       aTTGCTGGTAACTCACCCCCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTC‑GTAGCGACCGCACACCAGAAT‑CAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCTTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACcat                                                                                                                                                                                        <  1:86321/251‑1 (MQ=255)                                          gCTGGTACCTCACCACCACTGAGAACGTAATCTCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTGC‑GGAGCGACCGCACACCAGAAT‑CAATTTTTGATTCGTTGCCAGTCCGCTGACGCCCGCTTGATCAAGTTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACcatca                                                                                                                                                                                      <  1:55437/250‑1 (MQ=255)                                           cTGGTAACTCACCACCACTGAGAACGTAATCGCCGCTTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTC‑GTAGCGACCGCACACCAGAAT‑CAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACcatcctt                                                                                                                                                                                    >  1:17305/1‑248 (MQ=255)                                            gggTATCTCACCACCCCTGAGAACGTAATCGCCGATTGACCATTTTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATCCCTTC‑GTAGCGACCGCCCACCAGAAT‑CAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAACCACCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTa                                                                                                                                                                                   <  1:454889/250‑1 (MQ=255)                                              gTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTC‑GTAGCGACCGCACACCAGAAT‑CAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAAc                                                                                                                                                                                 >  1:388855/1‑251 (MQ=255)                                                      cccccccTGAGAACGTAATCGCCGATTGACCTTTCTTCGTCAATTTCGGTTTGGACCACGCGCTCACCTATACCTCC‑GTAGCGCCCGCACCCCAGAAC‑CAATTTTTGATTCGTTGCCAGTTCGCTGCCGCCCGCTTGATCAAGTTTGCGTCCGTGTGGTGACAGAAAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCgg                                                                                                                                                                         <  2:24925/245‑1 (MQ=255)                                                            cgtagaacgactgcGCCGATTGACCATTCTTGGACATTGACGGTTTGGATCGCGCGCTCTTCTATTCTGTC‑GTAGCGCCCGTACACCAGAAT‑CAATTTTTTATCCGTTGCCAGTTCGCTGACGCCCGCTGGATCATGCTGGCGGCCGTGTGGTGACAGATAACGCCCCTTGGCGCCGTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTccgcc                                                                                                                                                                   <  2:369940/238‑1 (MQ=255)                                                            cTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTC‑GTAGCGACCGCACACCAGAAT‑CAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATc                                                                                                                                                                              <  1:31313/240‑1 (MQ=255)                                                            cTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTC‑GTAGCGACCGCACACCAGAAT‑CAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATc                                                                                                                                                                              >  2:31313/1‑240 (MQ=255)                                                             tGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTC‑GTAGCGACCGCACACCAGAAT‑CAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTccgccg                                                                                                                                                                  >  1:369962/1‑251 (MQ=255)                                                               agaACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTC‑GTAGCGACCGCACACCAGAAT‑CAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTccgccgcc                                                                                                                                                                >  1:265693/1‑251 (MQ=255)                                                                          gcagATTGACCATTCTTCGACAATTTAGGTTTGGATAACGCGCTCATCTATACCTTC‑GTATCGACTGCACACCAGAAT‑CAGTTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGGAGGGCGTCCCGCAAGGGTTGCCCCATCGTTAACATCCCCAGTCCGCCGCCGTGAGGACGac                                                                                                                                                     >  2:72189/4‑250 (MQ=255)                                                                                ttaCCATTCTCCGTCAATTCTGGTTTGGCCCACGCGTTCCTATATCCCTTC‑GTAGCGCCCGCACCCCA‑ATTACATTTTTTGATTCGTTGCCATTTCGTTGACCCCCGCTTGTTCAGGCTTGCCCCCTTGTGTTGCAAAATAAACCCCCTTTCCCCCTTCCCCCGCGGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTGGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTcc                                                                                                                                                <  2:178124/248‑1 (MQ=255)                                                                                           cGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTC‑GTAGCGACCGCACACCAGAAT‑CAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGc                                                                                                                                    >  1:229514/1‑251 (MQ=255)                                                                                              cAATTTCGGTTTGGATCACGCGCTCATCTATACCTTC‑GTAGCGACCGCACACCAGAAT‑CAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTCACATCCCCGGTCCGCCGCCGTAAGGACGTTCGTCCACGGTCCGGTGcagg                                                                                                                                 >  2:252665/1‑248 (MQ=255)                                                                                                    cggttggacccccgcgCTCATCGACCCCTTC‑GTGGCGACCGCACACCAAAAT‑CAATTTTGAATTCGTTGCCGTTTCGCGGCCCCCCGTTTAAACAGGCTGGCGGCCCTGTGGTGACAGAAAAATCCCTTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATg                                                                                                                            <  1:138877/238‑1 (MQ=255)                                                                                                      gTTTGGATCACGCGCTCATCTATACCTTC‑GTAGCGACCGCACACCAGAAT‑CAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGt                                                                                                                         >  1:421836/1‑251 (MQ=255)                                                                                                        ttGGATCACGCGCTCATCTATACCTTC‑GTAGCGACCGCACACCAGAAT‑CAATTTTTGATTCTTTGCCAGTTCGCTGACGCCTGCTTGATCAAGCTTGCGTCCCTGTGGTGCCAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTACACCATCATTAACATCCCCGGTCCGCCGGCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTgg                                                                                                                       >  2:304183/1‑250 (MQ=255)                                                                                                               ccccccTCATCTACCCCTTC‑GAGGCCACCGCACCCCAGAAT‑AGTTTTTTGATCCGTTGCCAGTTCGTGGCCGCCCGCTTAATCAAGCTTGCCTCTCTGTGGTGACAGAAAAATCGCCTTGCCCCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTcgcg                                                                                                                <  1:289631/246‑1 (MQ=255)                                                                                                                cgcgCTCATCTATACCTTC‑GTAGCGACCGCACACCAGAAT‑CAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTcgcg                                                                                                                >  2:460416/1‑250 (MQ=255)                                                                                                                      cATCTAT‑CCTTCCGTAGCGACCGCACACCAAATT‑CATTTTTGGTTTCGTGGCCAGTTCGCTGACCCCCGCTTGTTCAAGCTCGCGTCCTTGTGGTGAGAGATAAAGCCCCTTGCCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGTTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGTTGCCGGTCATGCGTGAAGTCGCGAGGAc                                                                                                           <  1:413411/249‑1 (MQ=255)                                                                                                                       aTCTATACCTTC‑GTAGCGACCGCACACCAGAAT‑CAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGGGCCGGTCATGCGTGAAGTCGCGAGGACTcc                                                                                                        >  2:3042/1‑251 (MQ=255)                                                                                                                          tataCCTTC‑GTAGCGACCGCACACCAGAAT‑CACTTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGCTAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCACCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGc                                                                                                     >  2:288313/1‑251 (MQ=255)                                                                                                                          tataCCTTC‑GTAGCGACCGCACACCAGAAT‑CACTTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGc                                                                                                     >  1:321725/1‑251 (MQ=255)                                                                                                                                 tC‑GTAGCGCCCCCACCCCAGAAC‑CAATTTTTGATTCTTTGCCAGTTCGCTGACCCCAGCTGGATCAAGCTTGCGCCCCTGTGGTGACAGAAAAACCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTgg                                                                                                <  1:413820/249‑1 (MQ=255)                                                                                                                                 cc‑GTAGCGCCCGCCCCCCAAAAT‑CAATTTTTGATTCGTTGCCGGTTCGCTGACGCCCGCTTGATCAACCTGGCGTCCCTGTGGTAACAGATAAACCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTgg                                                                                                <  1:455306/248‑1 (MQ=255)                                                                                                                                         gACCGCACACCAGAAT‑CAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCACCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTcagc                                                                                       >  1:341928/1‑251 (MQ=255)                                                                                                                                          aCCGCACACCCGAAT‑CACTTTTTAATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGGGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACAATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTCCGGTGCCGGTCATGCGTGCAGTCGCGAGGACTCCAGCACTGGaggaccagc                                                                                       >  2:430121/1‑242 (MQ=255)                                                                                                                                                      aaT‑CAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTa                                                                           >  1:76449/1‑250 (MQ=255)                                                                                                                                                               tttGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACATTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGCAGTCGCGAGGACTCCAGCTCTTGCTGCTCAGCAGGCCCTTTTTCAATGCCCgg                                                                  >  2:799/1‑251 (MQ=255)                                                                                                                                                                      cGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTATGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGttc                                                           >  2:114172/1‑250 (MQ=255)                                                                                                                                                                            cAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCCGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCgg                                                     >  2:147936/1‑250 (MQ=255)                                                                                                                                                                             aGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGGGACAGATAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCCCGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCCGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGt                                                     >  2:418890/1‑250 (MQ=255)                                                                                                                                                                               ttCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGGGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGAGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCGCGGCCAGTTACCCCGTAAt                                                  >  1:39570/1‑251 (MQ=255)                                                                                                                                                                                           cGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGTAATTgcgc                                      <  1:219286/251‑1 (MQ=255)                                                                                                                                                                                                                       gACAGATAAACCCCCTTTGCCCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGTAATTGCGCGGAACATTTCAGGAAACAGGCTAATTa           <  1:405435/250‑1 (MQ=255)                                                                                                                                                                                                                               aaTCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGTAATTGCGCGGAACATTTCAGGAAACAGGCTAATTATGCCAATcc  >  1:143308/1‑251 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       CTCCCGGAATAAACCGGGAAACGGAGTCAATCAGCGTCATTGCTGGTAACTCACCACCACTGAGAACGTAATCGCCGATTGACCATTCTTCGTCAATTTCGGTTTGGATCACGCGCTCATCTATACCTTC‑GTAGCGACCGCACACCAGAAT‑CAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCGTAAGGACGATCGTCCACGGTACGGTGCCGGTCATGCGTGAAGTCGCGAGGACTCCAGCTCTGGATGCTCAGCAGGCCATTTTTAACTGCCCGGCCAGTTACCCCGTAATCGGTAATTGCGCGGAACATTTCAGGAAACAGGCTAATTATGCCAATCC  >  NC_000913/2744865‑2745335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑