Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,638,240	G→A	L21L (TTG→TTA)	creD →	putative inner membrane protein CreD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,638,240	0	G	A	100.0%	165.5 / NA	57	L21L (TTG→TTA)	creD	putative inner membrane protein CreD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/30);  total (27/30)

AGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGC  >  NC_000913/4637995‑4638486                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       agctgcGGTCGGGGGTGGGCGTTCGTTAGTGAGGTCGCCCGGTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAAGCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTcc                                                                                                                                                                                                                                                   <  2:141406/247‑1 (MQ=255)  gcagcGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCg                                                                                                                                                                                                                                                  >  2:515313/1‑251 (MQ=255)    agcGGACTGGGGTAGGCGTTCGTCAGTGAGGGCGCCCGTTTGTTTAACGGCGAAGACACGCTGCGCACCGTGCAGGAAGGAGGCGTGCTGGCCTCGCTTCGACTTCACCGTAACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATACTGCTGCCATTGCAAAGAAGAAGACTATGTTGAAATCACCCCTGTTCTGGAAAATGACTAGCCTGTTTGGAGCAATAGTGCTGTTGTTAATTc                                                                                                                                                                                                                                                    >  2:438937/1‑247 (MQ=255)       ggTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAAt                                                                                                                                                                                                                                             >  2:15571/1‑251 (MQ=255)            ggggTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCGGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGc                                                                                                                                                                                                                                           <  1:325037/248‑1 (MQ=255)            ggggGTGGCGTTCTGCAGGGAGGTCTCCCGTTTGTTTAACGGCGAATTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCCCATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGTCTATGTTGAAATCCCCCCTGTTCTGTAAAATGACTTGCCTGTTTGGTGCAGTATTGCTGGTGTTAATACCGATAATGCTGa                                                                                                                                                                                                                                        <  2:22656/251‑1 (MQ=255)                 tGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCgg                                                                                                                                                                                                                                   >  1:64360/1‑251 (MQ=255)                   gcgtgcgtCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCGGCGCAACGGGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTGCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTTGAAAATGACTAGCCTGTTTGGTGCAATATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCa                                                                                                                                                                                                                                 <  2:76319/251‑1 (MQ=255)                         gTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGAt                                                                                                                                                                                                                           <  1:374290/251‑1 (MQ=255)                          gcAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGAtt                                                                                                                                                                                                                          <  1:110545/250‑1 (MQ=255)                                    cGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTg                                                                                                                                                                                                                <  1:181171/251‑1 (MQ=255)                                      cccggttgggtaccGGCGAAGTCACGCAGCGCAACGTGCAGGAAGGTGGCTTGCTGGCTTCGCTTCGACTTCACCGTCACTTCTCATTGCGTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGaatttgct                                                                                                                                                                                                              <  2:374461/238‑7 (MQ=255)                                            tgtttAACGGCGAAGACACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTACTGGAAAATGACTAGCCTGTTTGGTGCAGAATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGCTTAc                                                                                                                                                                                                        >  1:319109/1‑251 (MQ=255)                                                 aaCGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGt                                                                                                                                                                                                     >  1:20565/1‑249 (MQ=255)                                                              tgCTGCGAAACGGCCAGGAAGGTGGCGTGCTGGCCTCTCTTCGACTTCACCGTCACTTCACATAGCTGCAAATTCTTCCCACATAGTCGTCGTATCCTGCTGCCATTGCATAGGAGAAGACTATGTTGAAATCCTCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTAGTGCTGTTGTTAATTCCGATAAGTCTGATTCGGCAGGTGATTGTCGAACGTGCGGATTTCCGGAGCGATTTGGAAGa                                                                                                                                                                                       <  2:502828/249‑1 (MQ=255)                                                                     cAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTAGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGCTGCGATTc                                                                                                                                                                               >  2:42843/1‑251 (MQ=255)                                                                              ggAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGt                                                                                                                                                                       >  1:339644/1‑250 (MQ=255)                                                                                    tGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCACCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAGCGTGCTGATTACCGTATCGATGTGGCAGATGCGATTCGCCAAAGTACCAGCg                                                                                                                                                                >  2:151854/1‑251 (MQ=255)                                                                                      gCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCggg                                                                                                                                                              <  1:515313/251‑1 (MQ=255)                                                                                        gTGCTGTCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGcc                                                                                                                                                            <  2:339644/251‑1 (MQ=255)                                                                                               ccTCGCTTCTACTTCCCCGTCACTTCACATGACTTCAAATTCGTCCCACATAGTCTTCGTACCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCGGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCaaaaa                                                                                                                                                     <  2:120374/251‑1 (MQ=255)                                                                                                   gCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCg                                                                                                                                                 >  1:168012/1‑251 (MQ=255)                                                                                                         ccTTCACCGTCACTTCACAGAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTTCAAAGTATAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGc                                                                                                                                           <  1:490582/250‑1 (MQ=255)                                                                                                            tCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGAAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGTAGATGCGATTCGCCCACGTACCAGCGGGCCGCAAAAACTCATTGGGACGc                                                                                                                                        >  2:90750/1‑251 (MQ=255)                                                                                                                cGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTAGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGCTGTGGAAGAGGCGATTAGCAAAAGAAACAGCTGTCCGCAACAGCTCGTTGGGCCgtccac                                                                                                                                    >  2:514583/1‑246 (MQ=255)                                                                                                                 gTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATc                                                                                                                                   >  1:134540/1‑251 (MQ=255)                                                                                                                 gTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTAGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATc                                                                                                                                   <  1:391789/251‑1 (MQ=255)                                                                                                                    aCTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGTGCCGCAAAAACTCGTTGGGCCGCTCATCGCt                                                                                                                                <  1:427235/251‑1 (MQ=255)                                                                                                                       tCACATAGCTGAAAAGTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTag                                                                                                                              <  2:444992/250‑2 (MQ=255)                                                                                                                        caaaTAGCTTCAAATTCTTCCCACAATGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCTCCTTTTCTGGAAAATTTCTAGCCTGGTTTGTGCAGTATTTCTGTTGTTAATTCCGATAATGCCGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCTATGTGGAAGATGCGATTCGCCAAATTACCAGCGTGCCGCAAAAACTCGTTGGGCCGCTCATCGCTAtt                                                                                                                             <  2:319109/247‑1 (MQ=255)                                                                                                                          attAGCTTAAAATTGTTCCCATATAGGTTGCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCTCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCTGCAGGTGATTGTCGAACGTGTTGATTACCGTAGCGATGTTGAAGATGCGATTCGCCAAAGTACCAGCTGGCTTCAAAAACTCGTTGTGCCGCTCAGCGCTATTCCt                                                                                                                          <  2:491371/249‑1 (MQ=255)                                                                                                                             aGCTTCAAATTTGTCCCGCATAGCCTTCGTATCCGGCTGCCATTGCAAAGGATAAGACTATGTGGAAATCCCCCCTGTTTTGGAAAATGACTAGCCGGTTTGGTTCAGGATTGCTGTTGTAAAGTCCGATAATGCTTGTTCGGCAGGTGATTGTCGATCGTGCTGATTTCCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAATCTCGTTGGGCCGCGCATCGCTATTCCtgt                                                                                                                        <  2:196089/250‑1 (MQ=255)                                                                                                                                        cttcCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGCCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGGACCAGCGGGCCGCAATAACTCGTTGGGCCGCTCATCGCCCTTCCTGTGACCGAGCttt                                                                                                             >  2:449302/1‑250 (MQ=255)                                                                                                                                         ttcCCACATAATCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTATCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGTACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCCCTATTCCTGTGCCCGAGCTTtat                                                                                                           >  1:182202/1‑251 (MQ=255)                                                                                                                                           cccACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGCTTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGACCTTtata                                                                                                          >  2:239465/1‑250 (MQ=255)                                                                                                                                             cacaTAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGCCCGAGCTTTATACgg                                                                                                       >  1:24226/1‑251 (MQ=255)                                                                                                                                                          tATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAagggg                                                                                           >  2:458749/1‑247 (MQ=255)                                                                                                                                                           aTCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGAt                                                                                         <  1:128822/251‑1 (MQ=255)                                                                                                                                                                         gCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCg                                                                           <  1:165952/251‑1 (MQ=255)                                                                                                                                                                           aaaGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCgg                                                                          >  1:69398/1‑250 (MQ=255)                                                                                                                                                                            aaGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGaa                                                                        <  1:236063/251‑1 (MQ=255)                                                                                                                                                                                 gaagaCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGaa                                                                   <  1:15571/251‑1 (MQ=255)                                                                                                                                                                                  aagatgaTGTTGAAATCCCCCCTTTTGTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATGCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAGGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTAGTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAg                                                                  <  2:182907/245‑1 (MQ=255)                                                                                                                                                                                       tATGTTGAAATCCCCCCTGTTCTGGAAAATGAATAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGtttt                                                              >  1:193311/1‑250 (MQ=255)                                                                                                                                                                                        aTGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTAt                                                            <  1:458749/251‑1 (MQ=255)                                                                                                                                                                                            tGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCAt                                                        >  1:386241/1‑251 (MQ=255)                                                                                                                                                                                             gAAATACCCCCTGTTCTGGAAAAGGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATCCCGATAATGCTGAATCGGCAGGTGATTGTCGAACGTGCTGATTCCCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTCCCCGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACAGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCAtt                                                       <  1:231/251‑1 (MQ=255)                                                                                                                                                                                                 tCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTg                                                   >  1:112041/1‑251 (MQ=255)                                                                                                                                                                                                       cTGTTCTGGAAAATTACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTAcc                                             >  1:54226/1‑251 (MQ=255)                                                                                                                                                                                                         gTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGCTGTGGAAGATGCGATTCGCCAAAGTTCCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGATATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGTAGTTTTATCCTTTTTTGGTTACCTg                                           >  2:512969/1‑251 (MQ=255)                                                                                                                                                                                                         gTTCTGGAAAATGACAAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTg                                           >  2:418609/1‑251 (MQ=255)                                                                                                                                                                                                                              gTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGc                       <  1:449302/250‑1 (MQ=255)                                                                                                                                                                                                                                      cAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATATGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAAc              >  2:95188/1‑251 (MQ=255)                                                                                                                                                                                                                                       aGTATGGCTGGTGTTAATTCCGAAAATGCTGAGTCGGCAGGTGATTGTCGAACGTGCTGATTTCCGTAGCGATGTGGAAGATGCGATTCGCAAAAGGACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACg             <  2:20565/251‑1 (MQ=255)                                                                                                                                                                                                                                        gTATTTCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATTTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGt            <  2:174191/251‑1 (MQ=255)                                                                                                                                                                                                                                        gTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGt            <  1:438937/251‑1 (MQ=255)                                                                                                                                                                                                                                              cTGTTGTTAATTCAGATAATGCTGATTCGGCAGGAGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGGTGATGGCATTCAGaaaatgga         >  1:275882/1‑242 (MQ=255)                                                                                                                                                                                                                                                  tgttaATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGc  >  1:466027/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       AGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGC  >  NC_000913/4637995‑4638486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑