Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,636,925	G→C	R77P (CGC→CCC)	creC →	sensory histidine kinase CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,636,925	0	G	C	100.0%	169.6 / NA	49	R77P (CGC→CCC)	creC	sensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (19/30);  total (19/30)

GAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGAC‑‑GTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCG‑TCCC‑GATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGACG  >  NC_000913/4636684‑4637164                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                gAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGAC‑‑GTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCG‑TCCC‑GATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTcg                                                                                                                                                                                                                                        >  1:515325/1‑251 (MQ=255)       ccTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTTGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGCAGAGCAACGGAGGGGAC‑‑GTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCG‑TCCC‑GGTTTGCTCTCTGGGGGCCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGGTTCGCGCCa                                                                                                                                                                                                                                   >  1:357619/1‑250 (MQ=255)                cGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGAC‑‑GTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCG‑TCCC‑GATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACAACCCCCGATTCGCGCCAATATCGGTgg                                                                                                                                                                                                                         >  1:368768/1‑251 (MQ=255)                   aTCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGAC‑‑GTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCG‑TCCC‑GATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCAt                                                                                                                                                                                                                      <  1:86992/251‑1 (MQ=255)                             ggTTATTGCTGGGCTATTTTTTACTGGTGGCGGTGGAAGCCTGGGTCGGACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGAGGGCGAAACGCCACGGAGAGGAC‑‑GTTGATCGACACCGCCCCGTTGCTGGCGGGGATAGCGCG‑GCCCTGATT‑GCTCTCTGGGTGCCCCACGAATAGGCAAATTGCGCAGGCGTTTTATCAGCTACAACATCCCCCGATTCCCGCCAATATCGGAGGccatgactaaatg                                                                                                                                                                                                            >  2:25857/1‑239 (MQ=255)                                        ggCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGAC‑‑GTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCG‑TCCC‑GATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAata                                                                                                                                                                                                 <  1:495981/251‑1 (MQ=255)                                               ttttACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGAC‑‑GTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCG‑TCCC‑GATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGt                                                                                                                                                                                           <  1:397728/250‑1 (MQ=255)                                                      ggtggcggtggcAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGAC‑‑GTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCG‑TCCC‑GATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCCATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATg                                                                                                                                                                                    >  1:454001/1‑250 (MQ=255)                                                           gggtggcAGGCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGAAACGGAGGGGAC‑‑GTTGAGCGACCCCGCAACGTTGCTGGCGGAGCTGGCGCG‑TCCC‑GATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCgct                                                                                                                                                                              <  2:452976/250‑3 (MQ=255)                                                             gtggcAGCCTGGGTCGTACTGGCCATGTTTGTCAGAGAAGGTCAGCCGTGCGTGCTAATAGCAACGGAGGGGAC‑‑GTTGACTGACACCGCAACGGTGCGGGGGGAGCTGGCGCG‑TCCC‑GATGTGCTCTCGGGGGACCCAACGGATGGGCAACTGGCGCAGGCGTTTAATCAGCTACTACATCCCCCGTTTCACGCCAATATCGGTGGCATTAACAAAGTTCGCAATGAATATCAGGTCTATATGACCGATgc                                                                                                                                                                            <  2:311517/251‑1 (MQ=255)                                                               ggcATCCTGGTTCGTACTTGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCATCGGAGGGGAC‑‑GTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCG‑TCCC‑GATTTGCTCTCTGTGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATgcg                                                                                                                                                                           <  1:367342/250‑1 (MQ=255)                                                                  aGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGAC‑‑GTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCG‑TCCC‑GATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGGTTCGCGCCAATATCGGTTGCATTAACAAAGTGCGCAATGAATATCAt                                                                                                                                                                                             <  1:415426/229‑1 (MQ=255)                                                                  aGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGAC‑‑GTTGATCGACACCGCAACGTTGCTGGCGGAGATGGCGCG‑TCCC‑GCTTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACCAAGGGCGCACTGACTATCAtcgaaaggaagagcgtcgtgt                                                                                                                                                                         >  2:415426/1‑229 (MQ=255)                                                                   gCCTGGTTCGTACTGGTCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGAC‑‑GTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCG‑TCCC‑GATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAggg                                                                                                                                                                      <  2:126521/251‑1 (MQ=255)                                                                    ccTGGTGCGTACTGGCTATTGTTGTCAAAGAAGTTAATCCGGGCGTGCGATGAGCAACGGAGGGTAC‑‑GTTGATCGACACCGCAACGTTGCTGGCGGATCTGGCGCT‑TCCC‑GATTTGCTCTCTGGGGACCCAACGCATGGGCAACGGGCGCAGGCTGTTAATCAGCTACAACGTCCCCCGTTTCGCGCCAATAGCGGTGGCATTACCAGAGTGCGCAATGAATATCATGGCTGTATGACCGGTGCGCAGGGc                                                                                                                                                                     <  2:80021/251‑1 (MQ=255)                                                                        gTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGAC‑‑GTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCG‑TCCC‑GATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCaa                                                                                                                                                                   >  1:529378/1‑249 (MQ=255)                                                                              ccTGGCCACTGTTGTCAAAAAAGTTAACCCGGGCGGGAGCAGAGCAATGGAGGGGACGAGTTG‑‑CGACTCCGTAACTTTGCTGGCGGACCTGGCGCG‑TCCC‑GAGTTGATCTCTGTGTACCCAACGCATGGGCAACTGGCGCGGGCGTGTAATCAGCTACAACACCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAAGATCATGTCTATATGACCGAGGCGCAGGGCAACGTATTg                                                                                                                                                            <  2:135658/249‑1 (MQ=255)                                                                                   ccATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGAC‑‑GTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCG‑TCCC‑GATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGAt                                                                                                                                                      >  1:188993/1‑251 (MQ=255)                                                                                     aTTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGAC‑‑GTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCG‑TCCC‑GATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTc                                                                                                                                                    <  1:534338/251‑1 (MQ=255)                                                                                                 gaagTTAAACCGGGCGGGCGAAGAGCAACGGAGGGGAC‑‑GTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCG‑TCCC‑GATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGc                                                                                                                                        <  1:227178/251‑1 (MQ=255)                                                                                                   agTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGAC‑‑GTTGATCGACACCGCAACGTTGCTGGCGGAGATGGCGCG‑TCCC‑GATTTGCTCTCTGGGGACCCAAAGCATGGGCAACTGGGGCAGGCGTTTAATCAGCTACAACATCCCCAGTTTCGCGCCAATATAGGTGGCATTTACAAAGTGCGCAATGAATATCTTGTCTATATGACCGATGCGACGGGCAAAGGATTGTTAGATTTGGCAAATAAAGCCg                                                                                                                                      >  1:5031/1‑251 (MQ=255)                                                                                                    gTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGAC‑‑GTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCG‑TCCC‑GATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGt                                                                                                                                     >  1:190827/1‑251 (MQ=255)                                                                                                       aaaCCGGGCGTGCGAAGAGCAACGGAGGGGAC‑‑GTTGATCGACACCGCAACGTTGCTGGCGTAGCTGGCGCG‑TCCC‑GATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTgg                                                                                                                                  <  2:94026/251‑1 (MQ=255)                                                                                                       aaaCCGGGCGTGCGAAGAGCAACGGAGGGGAC‑‑GTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCG‑TCCC‑GATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTg                                                                                                                                   <  2:174256/250‑1 (MQ=255)                                                                                                              gCGTGCGAAGAGCAACGGAGGGGAC‑‑GTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCG‑TCCC‑GATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGat                                                                                                                           <  1:229028/251‑1 (MQ=255)                                                                                                               cGTGCGAAGAGTAACGGAGGGGAC‑‑GTTTATCGACACCGCAACGTTGCTGGCTGAGCTGGCGCT‑TCCC‑GATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATTAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATGCGGCAAATAAAGCCGTTGGACAGGatt                                                                                                                          <  2:403438/251‑1 (MQ=255)                                                                                                                  gCGAAGAGCAACGGAGGGGAC‑‑GTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCG‑TCCC‑GATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGattat                                                                                                                        <  1:44266/250‑1 (MQ=255)                                                                                                                          cAACGGAGGGGAC‑‑GTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCG‑TCCC‑GATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTc                                                                                                                                                    <  1:213098/214‑1 (MQ=255)                                                                                                                          cAACGGAGGGGAC‑‑GTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCG‑TCCC‑GATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTc                                                                                                                                                    >  2:213098/1‑214 (MQ=255)                                                                                                                              ggAGGGGAC‑‑GTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCG‑TCCC‑GATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGGATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATg                                                                                                           >  1:257028/1‑251 (MQ=255)                                                                                                                                gggggAC‑‑GTTGATCGAAACCGCAACGTTGCTGGCGGAGCTGGCGCG‑TCCC‑GAGTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAAGCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGAc                                                                                                         <  2:519730/250‑1 (MQ=255)                                                                                                                                 ggggCC‑‑GTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCG‑TCCC‑GATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGAGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATCAAGCCGTTGGACAGGATTCTTCGCGCTGGAATGACg                                                                                                        >  1:269752/1‑251 (MQ=255)                                                                                                                                                    ccGCAACGTTGCTGGCGGAGCTGGCGCG‑TCCC‑GATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGt                                                                                       <  1:69389/251‑1 (MQ=255)                                                                                                                                                     cGCAACGTTGCTGGCGGAGCTGGCGCG‑TCCC‑GATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTAGCGTg                                                                                      <  1:119599/251‑1 (MQ=255)                                                                                                                                                      gCAACGTTGTTGGCGGAGCTGGCGCG‑TCCC‑GATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTgg                                                                                     <  1:334380/251‑1 (MQ=255)                                                                                                                                                       aaacgttgcagcgggAGCTGG‑GCGGTCCC‑GTTTTGCCCGCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCTGTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGGTCGATTCGGCAAATAAAGCCGTTGGACAGGAATATTCGCGCCGGAATGGCGTCTGGCTAACGTTTCGTGGt                                                                                    <  2:113197/238‑1 (MQ=255)                                                                                                                                                          cGTTGCTGGCGGAGCTGGCGCG‑TCCC‑GATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGG‑CAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTggagatcggaagggcgtcggg                                                                                >  2:225517/1‑232 (MQ=255)                                                                                                                                                          cGTTGCTGGCGGAGCTGGCGCG‑TCCC‑GATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGG‑CAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTgg                                                                                                   <  1:225517/232‑1 (MQ=255)                                                                                                                                                              gcgggCGGAGCTGGCGCG‑TCCC‑GATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATg                                                                             <  2:515325/248‑1 (MQ=255)                                                                                                                                                              gCTGGCGGAGTTGGCGTG‑TCCC‑GATTTGCTCGCTGGGGACCTAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCTGTTGGCCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTAt                                                                              <  1:346598/250‑1 (MQ=255)                                                                                                                                                                  gCGGAGCTGGCGCG‑TCAC‑GCTTTGATCTCTGGGGACCCAACGCATGGGCAACTGGTGCAGGAGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATAGCGGTGGCATTAACAAAGTGCGCTATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGATGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTgc                                                                         <  1:496702/251‑1 (MQ=255)                                                                                                                                                                   cGGAGCTGGCGCG‑TCCC‑GATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCACAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACCGGATTATTCGCGCTGGAATGACGTCTGGATAACGTTGCGTGGTCAGTATGGTgcg                                                                        >  2:73754/1‑251 (MQ=255)                                                                                                                                                                            cgcg‑TCCC‑GATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCTCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCATCACg                                                               <  2:508189/251‑1 (MQ=255)                                                                                                                                                                                 tCCC‑GATTTGCTCTCTGTGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGc                                                           >  1:347764/1‑251 (MQ=255)                                                                                                                                                                                      gATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACACCATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGCCGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCaca                                                        >  2:163397/1‑248 (MQ=255)                                                                                                                                                                                                                   gCAACCGGCGCAGGCGTTTAATCAGCTACAAAATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAATTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAATTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGTCTAACGTGGCGTGGTCAGTATGGTTCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGatgt                          <  2:540214/251‑1 (MQ=255)                                                                                                                                                                                                                     aaCTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGatgtat                        >  1:526774/1‑251 (MQ=255)                                                                                                                                                                                                                        tGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGCTGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGCTTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGCTCCCGAACGTTCTTTGATGTATGtt                     >  2:201531/1‑251 (MQ=255)                                                                                                                                                                                                                                           gCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGACg  <  2:454001/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                GAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGAC‑‑GTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCG‑TCCC‑GATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGACG  >  NC_000913/4636684‑4637164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑