| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,585,480 | G→A | Q428* (CAA→TAA) | hsdR ← | type I restriction enzyme EcoKI endonuclease subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,585,480 | 0 | G | A | 93.4% | 161.0 / 2.8 | 62 | Q428* (CAA→TAA) | hsdR | type I restriction enzyme EcoKI endonuclease subunit |
| Reads supporting (aligned to +/- strand): ref base G (2/2); new base A (26/31); total (29/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.37e-01 | |||||||||||
GCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCAT‑CGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTG > NC_000913/4585233‑4585719 | gcCTGTTCGCCCAGAGAACGGCGGTCGACAAGGAAGAGAATGCGGTTCAAACCCTCGGACTGGATCAGGCGGCACATCATGGCGATTTGCGGAAGGGGTTTACCCGTACCAGTCGCCATACACC‑GCAGGAACTCTTGTTGCCCCTTGACGGTTTCCTTTTTTAACGCGCGGACGGCATCTTCCTGATTACAAAGCACGCCCAGCCCGCTCATGCCAAGGTGTAT‑GGCACACCACTGTTTCAGTTTTTACgg > 2:191670/1‑251 (MQ=255) gTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCAGGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCAT‑CGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCAGCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTACGGTTCg < 2:532871/251‑1 (MQ=255) cGCCAAGAGAACGGTGGTCGACAAGGAAGAGAATGCGGTTAAAACGCTGGGACTGGATCAGGCGGAACATCATTGCGATTGCCGTCCGGGTTTTACCGGTACCGGTCGCCAT‑CGCCAGCAGGATCTCTTGTTGCCCCTTGACGAGTGCCTTTGCAACCGCGCGGACGGCTTCTTCCGGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCt < 2:533926/250‑1 (MQ=255) ccAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCAT‑CGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGcc < 2:539779/251‑1 (MQ=255) cAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCAT‑CGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCg < 1:115658/251‑1 (MQ=255) tCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGATACCGGTCGCCAT‑CGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCcagc > 1:79315/1‑249 (MQ=255) gACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCAT‑CGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGT‑TATCGGCAAACCACTGCTTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGct > 1:44163/1‑251 (MQ=255) cAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCAT‑CGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCcaggatcgg > 1:136199/1‑244 (MQ=255) cAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCAT‑CGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCca < 2:136199/243‑1 (MQ=255) agaATGCGTATAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCCGAACGGGTTTTACCGGTACCGGTAGCCAT‑CGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCCTCTTCATGATAATAACGCAGGCCCAGATCGCTCATGCCCGGGG‑TATCGGCCACCCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCg > 2:497991/1‑251 (MQ=255) gaATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCAT‑CGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCgg > 1:256685/1‑251 (MQ=255) aaTGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCAT‑CGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGt < 1:348946/251‑1 (MQ=255) cGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCTGTACCGGTCGCCAT‑CGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCAGCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCAAGCAGCTCTTCCGGGCGGTGCCa < 1:317116/251‑1 (MQ=255) aaaaCGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCAT‑CGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCCGCTATTCCGGGCGGTGCCACTCgg > 1:454545/1‑251 (MQ=255) gCTGGGACTGGATCAGGCGGAACAGCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCAT‑CGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAg < 1:197929/251‑1 (MQ=255) tGGGACTGGATCAGGCGGAACATCATGGCGATTGCCGGACGGGTTTTACCGGTACCGCTCGCCAT‑CGCCAGCCGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGT‑TATCGGCCACCAACTGATTCTGTCTTTAAGGTTCGCTGCCGAGCTTTTCCTGCAGCACTTCCTGGCGTGGCCACTCgggcacgac > 2:391066/1‑245 (MQ=255) ggACTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCAT‑CGCCAGCAGGATCTCTTGTAGCCCCTTGACGATTGCCTTTTCAACCGCGCAGACGACATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGT‑TATCGGCAAACCTCTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTGCAGCATCTGTTCCGGGCGGGGCCGCTCGGGGAAGTCtt > 2:101991/1‑251 (MQ=255) gACTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCAT‑CGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGTGCGGTGCCACTCGGGTAAGGCttt < 1:435090/251‑1 (MQ=255) gACTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCAT‑CGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCttt < 2:150668/251‑1 (MQ=255) cTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCAT‑CGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCg > 1:48799/1‑251 (MQ=255) ggctCAGGCGGAACATCATGGCGATTGCCGGACGGGTTTTACCGGTACCGGTCGCCAT‑CGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGTCCCAGCTCGCTCATGCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGAc < 2:9858/248‑1 (MQ=255) aTCAGGCGGAACATCATGGCGATTGCCGTACGGGATTTACCGGTACCGGGCGCCAT‑CGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGAGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCCTGCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCCTTTCCAGCAGCCCTTCCGGGCGGGGCCACTCGGGAAAGGCTTTCGaaat > 2:301425/1‑248 (MQ=255) aGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCAT‑CGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAAAGCAGGCCCAGCTCGCTCATGCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATAtt > 2:348817/1‑251 (MQ=255) ggcaCATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCAT‑CGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTCCGGTTCGCTGCCGAGCATTTCCAGCAGCACTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACCTCTTGCgg > 2:96327/4‑251 (MQ=255) ggAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCAT‑CGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTATGGCTTTCGACATATTGCgg > 2:293278/1‑251 (MQ=255) atcatGGCGATTGCCGTACGGGTTTTACCGGTACCGGGCGCCAT‑CGCCAGCAGGATCTCTTGTTGCCCCTTGACGAATGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGATCATGCCAGGGT‑TATCGGCAAACCAATGATTCTGTTTTTACGGTTCGCTGCAGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAGGGGTTTCGACATATTGCGGGAATc > 1:280361/1‑251 (MQ=255) tGCCGTACGGGTTTTACCGGTACCGGTCGCCAT‑CGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCTAGCTCGCTCATGCCAGGGT‑TATCGGCAAACTACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTcg < 2:319668/250‑1 (MQ=255) gttgtaccggttccggTCGCCAC‑CGCCAGCTGGATCTCTTGTTGCCCCTGGCCGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCGCGCTCATGCCAGGGT‑TATCGGCAAACCCCTGATTCTGTTTTTACGGTGCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACGCGGGTAAGGCTTTCGACATATTGCTGGTATCACGCACGTCGCTATACCAGAt < 2:327461/247‑1 (MQ=255) tttACCGGTACCGGTCGCCAT‑CGCCAGCAGGATCTCTTGTTTCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATg < 1:485543/250‑1 (MQ=255) ggcgccat‑cgccaGCAGGATCTCTTGTTGCCACTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGACCAGCTCGCTCATGCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCtt < 1:425376/249‑1 (MQ=255) tcgccat‑cgccaGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGGAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTc > 1:463324/1‑251 (MQ=255) ccat‑cgccaGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCAtt < 1:410845/251‑1 (MQ=255) cat‑cgccaGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCAttg < 1:326998/251‑1 (MQ=255) cgccaGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCAttgttg < 1:301425/251‑1 (MQ=255) ccaGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTgcg > 2:467094/1‑251 (MQ=255) agcagGATCTCTTTTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGcgcg > 1:229660/1‑251 (MQ=255) agGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTa < 1:445200/251‑1 (MQ=255) ttttCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAggg > 1:435398/1‑251 (MQ=255) ttCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAgggg < 1:238316/250‑1 (MQ=255) aaCCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGAttt < 1:275377/251‑1 (MQ=255) ggACGGCATCTTCCTGATAATCACGCAGGCCCTGCTCGCTCATGCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTAATTGTTGCGAGGTATCAGCGCCAGTTGGTCGAGACGCAGAAGGGGATTTTacaacgt > 2:315685/1‑245 (MQ=255) cGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCgttgt > 1:145248/1‑251 (MQ=255) tCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAgggg > 1:167116/1‑231 (MQ=255) tCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAgggg < 2:167116/231‑1 (MQ=255) cttgcTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGT‑TATCGGCAAGTCACTGATTCTGTTTTTACGGTTCGCGGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATcacgcac < 1:505148/150‑1 (MQ=255) cTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGT‑TCTCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATcacgccc > 2:505148/1‑152 (MQ=255) tCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCg < 1:400791/249‑1 (MQ=255) tgataagaaatcaagCCCAGCCCGCTCATGCCAGGGT‑TATCGGCAAAACACTGATTCTGGTTTTACGGTTGCCTGCCGCGCCTTTCCCGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCCCGTCGCGATACCAGCTGCAGCTTTTGGTCTTCATTGTTGAGCGGAATTCTCGCCAGTTAGTCGGGTGGCAGAAGGGGATTTTAAACCATTGGTTACCGcgtgt > 2:79839/15‑247 (MQ=255) gATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGgtg > 1:444420/1‑251 (MQ=255) cGCAGGCCCAGCTCGCTCATGCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCa < 1:172245/251‑1 (MQ=255) gggCCCAGCTCGCTCATGCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCt < 2:98413/250‑1 (MQ=255) cagcgtgctcATGCCAGGGT‑TATCGGCAAATCACTGATTCTGTTTGTACGGTTCGCTGCCGAGCATTTCCATCAGCTCTTCCGGGCGGTGGCTCTCGGGTACGGCGTTCGACATATTGCGGGTATCCCGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTATCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTTgtgtacttcaatctggttg < 2:385299/245‑16 (MQ=255) gctcgctcATGCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCa < 1:205791/251‑1 (MQ=255) cATGCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGCAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCTGCTGGTTTCTTACTCTg > 2:545127/1‑251 (MQ=255) gCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTTCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGc < 1:179138/250‑1 (MQ=255) tATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTc < 2:256685/250‑1 (MQ=255) accGGCAAACCACTGATTCTTGTTTTACGGTTCGCTGCCGAGCATTGCCAGCAGCTCTTCCGGGCGGGGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCAt < 2:483137/249‑1 (MQ=255) aTCGGCAAACCACTAATTATGTTTTTACGGTTCGCTGCAGAGCCTTACCAGCAGCTCTTCCGGGCGGAGCCTCTCGGGTAAGGCTTTCGACGTATTGCGGGTATCACGCACGTCGAGCTACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTAGTCGAGTAGCAGAAGGGGTTTTTAAACCATGGTTTGCCGCTGGTTGCCTGCAAGCTGGTTTCATACTCTGGCCCTGCTTCAt > 2:412779/1‑251 (MQ=255) gCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGATCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCATGCAc > 1:154797/1‑251 (MQ=255) aaCCACTGATTCTGTTTTTACGGTTCTCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGGGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTc < 2:458284/251‑1 (MQ=255) gATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCg < 1:128079/249‑1 (MQ=255) tCTGATTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGAAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGAGCCCGTTGGTCGAGTAGCAGAATGGGATTTTAAACCGTTGTTTTCCGCTGGTGTCCTGCCAGCTGGTTTCAAACTCTGGCACTGCTTCATGCACTTCATCCgggt > 2:525832/1‑247 (MQ=255) | GCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCAT‑CGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGT‑TATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTG > NC_000913/4585233‑4585719 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |