| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,425,696 | C→A | N59K (AAC→AAA) | priB → | primosomal replication protein N |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,425,696 | 0 | C | A | 96.0% | 199.1 / ‑3.8 | 75 | N59K (AAC→AAA) | priB | primosomal replication protein N |
| Reads supporting (aligned to +/- strand): ref base C (2/1); new base A (31/41); total (33/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
CGTCGCGATGATTTCGCAAACGAAACCGCTGATGATGCTGAAGCTGGGGATTCTGAAGAGTAATTTCTGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACACGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGG‑ACTGAGCAAAATGGTTTTGCATGCCGAG‑CAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACC > NC_000913/4425451‑4425940 | cggcgcgATGATTTCGCAAACGAAACCGCTGATGATGCTGAAGCTGGGGATTCTGAAGAGTAATTGCTGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCCCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGc < 2:142562/248‑1 (MQ=255) gcgATGATTTCGCAAACGAAACCGCTGATGATGCTGAAGCTGGGGATTCTGAAGAGTAATTTCTGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCAtt < 1:526631/251‑1 (MQ=255) cgATGATTTCGCAAACGAAACCGCTGATGATGCTGAAGCTGGGGATTCTGAAGAGTAATTTATGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTATGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGGCAAATGCCCGTTATTTTTAGCGGAAACGAAAAACAGGCCAtt > 2:398587/1‑250 (MQ=255) aCGAAACCGCTGATGATGCTGAAGCTGGGGATTCTGAAGAGTAATTTCTGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGTGGAAACGAAAAACAGGCCATTACTCACAGTATAACg > 1:494984/1‑251 (MQ=255) cGAAACCGCTGATGATGCTGAAGCTGGGGATTCTGAAGAGTAATTTCTGATTACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACgg < 2:536576/251‑1 (MQ=255) aaaCCGCTGATGATGCTGAAGCTGGGGATTCTGAAGAGTAATTTCTGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTc < 1:312994/251‑1 (MQ=255) aaaCCGCTGATGATGCTGAAGCTGGGGAGTCTGAAGAGTAATTTCTGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATGCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGTTTTCACCTGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACgttc < 1:312980/251‑4 (MQ=255) ccGCTGATGATGCTGAAGCTGGGGATTCTGAAGAGTAATTTCTGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTACATCGGGAATTCCTCACTGCAAGTTCGTGCTTGAGAATAGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGACAGGCGGGGTGTCAAATGCCCGTTATTGTTAGCGGAAAGGAAAACCAGGCCATTACTCAAAGTATAACGGTCgc > 1:98568/1‑249 (MQ=255) cGCTGATGATGCTGAAGCTGGGGATTCTGAAGAGTAATTTCTGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCa < 1:377232/251‑1 (MQ=255) cGCTGATGAGGCTGAAGCTGGGGATTCTGAAGAGTAATTTCTGATGCCCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATGCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCa < 2:170218/251‑1 (MQ=255) gatgatGCTGAAGCTGGGGATTCTGAAGAGTAATTTCTGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGt < 1:447047/249‑1 (MQ=255) gatgatGCTGAAGCTGGGGATTCTGAAGAGTAATTTCTGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGt < 1:494411/249‑1 (MQ=255) gatgatGCTGAAGCGGGGGATTCTGAAGAGTAATTTCTGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGt < 2:225939/249‑1 (MQ=255) gCTGAAGCTGGGGATTCTGAAGAGTAATTTCGGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATaa < 2:395795/250‑1 (MQ=255) aaGCTGGGGATTCTGAAGAGTAATTTCTGATGACCAACCGTCTTGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGtt < 1:236755/251‑1 (MQ=255) gCTGGGGATTCTGAAGAGTAATTTCTGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCa < 1:485533/251‑1 (MQ=255) gAAGAGTAATTTCTGAGGACCAACCGTCTGGGGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGATAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTTAGGGGttcattt < 2:434650/250‑1 (MQ=255) gagTAATTTCTGATGACCAACCGTCTGGCGTTGTCCGGCACCGTGTGCAGGGCTCCACATCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGCAAAACAGGCCATTACTCACAGTATACCGGTCGGCAGTCGCATAAACGTTCAGGGGttcctttcatt > 2:256479/1‑250 (MQ=255) aaTTTCTGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGCAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCcac < 1:168542/251‑1 (MQ=255) aCCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGCAAGGTCAGTCCATCAGGCATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGCAACGAAAAAAAGGCCATTACTCAACGTATTACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCCCAAGGCaaaaa > 2:27532/1‑248 (MQ=255) ccAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACACGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCCTTTCATGCCAAAAGGCAAAGAAc > 2:290861/1‑251 (MQ=255) ccAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAAc > 1:104248/1‑251 (MQ=255) ccAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAAc < 1:384830/251‑1 (MQ=255) ccAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAAc < 2:50650/251‑1 (MQ=255) ccAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGACAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAAc > 1:345621/1‑251 (MQ=255) cAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAACGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACg > 1:36694/1‑251 (MQ=255) cAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTAGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCAGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCAATTACTCCCAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACCAGGCCAAGAACg > 2:25321/1‑251 (MQ=255) gTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGG‑ACTg < 1:342635/251‑1 (MQ=255) tCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGACCGG‑ACTg > 1:519313/1‑250 (MQ=255) cTGGTGTTGTCCAGCACCGTGTGCAGGGCTCCCCTTCGACAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGAATAACGGTCGGCAGTCGCAAAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGG‑ACtagg > 2:435646/1‑248 (MQ=255) cTGGAGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCCGTAGCATAACCGTTCAGGGGTTCCTTTCATTCCACAAGGCAAAGAACggcttg > 2:295384/1‑245 (MQ=255) ggtggtGTCCGGCACCGTGTGCAGGTCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGATGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGTCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGG‑ACTGAg < 1:509625/249‑1 (MQ=255) ggTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGG‑ACTGAg < 1:95183/249‑1 (MQ=255) ggTGTTGTCCGGCACCGGGTGCAGGGCTCCCCTGCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCGTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGG‑ACTGAg < 2:128428/249‑1 (MQ=255) gtgtTGTCCGGCACCGTGTGCAGTGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCACGCCAAAAGGCAACAAAAGG‑ACTGatca > 2:117281/1‑247 (MQ=255) cGGCACCGGGTTCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTAACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGG‑ACTGAGCAAAATGGttt < 2:291113/251‑1 (MQ=255) cccGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGG‑ACTGAGCAAAATGGTTTTGCa < 2:246340/250‑1 (MQ=255) tgCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGGACGG‑ACTGGGCAAAATGGTTTTGCATGCCg > 2:167763/1‑250 (MQ=255) aGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGG‑ACTGAGCAAAATGGTTTTGCATGCCGAG‑Ca < 1:68328/251‑1 (MQ=255) gggCTCCCCTTCGAAAGGTCAGCCCTGCAGGATTTCCTCCCTGCCAGTTCGGGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGG‑ACTGAGCAAAATGGTTTTGCATGCCGAG‑CAg < 2:177196/251‑1 (MQ=255) gggCCCCCCCTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGG‑ACTGAGCAAAATGGTTTTGCATGCCGAG‑CAg < 2:292664/251‑1 (MQ=255) ccccTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGG‑ACTGAGCAAAATGGTTTTGCATGCCGAG‑CAGattg < 1:289180/250‑1 (MQ=255) ccccTTCGAAAGGTCAGTCCATCAGGAATTACTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGCAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCCCAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCCTTTCATGCCACAAGGCAAAGAACGG‑ACCACGCCACATGGTTTTGCATGCCGGG‑CAGcttg > 2:240581/1‑250 (MQ=255) tCGAAAGGTCAGGCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGGGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGG‑ACTGAGCAAAATGGTTTTGCATGCCGAG‑CAGattgaattg < 2:104248/250‑1 (MQ=255) cGAAAGGTACGTCCATCAGGAATTCCTCACTGCCCGTTCGTGCTTGAGCATCGTTCTGTGCAGGCGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGCAAAAAAGGCCATTACTCACAGTATAACGGTAGGCAGTCGCATAAACGGTCAGGGGTTCATTTCTTGCCACAAGGCAAAGACCGG‑GCTGAGCAAAATGGTTTTGACTGCCGAG‑CCGATTGAATTGAt > 2:319949/1‑251 (MQ=255) aaaGTTCAGTCCATCAGGAATTCCTAACTGCTAGTTAGTGATTGAGCATCGTTATGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGGGGGGTCAAATGCCAGTTATTGTTAGCGGAAACGAAAAATAGGCCATTACTCACAGTATAAAGGTCGGCATTCGCATAACCGATCAGGGGTTAATTTCCTGCCACAAGGCAAAGCACGGGAC‑GAGCAAAATGGTTTTGCATGCCGCGACA‑attgaattgagt > 2:110259/1‑248 (MQ=255) ggTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGCAAAGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGACGCATAACCGTTAAGGGGTTCAATTCCTGCCCAAAGGCAAATAACGG‑ACAGAGCCAAATGGTTTTGCATGCCGAG‑CAGATTGAATTGATAGAtt > 2:364882/1‑251 (MQ=255) cAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCAGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGGTCATTTCATGCCACAAGGCAAAGAACGG‑ACTGAGCAAAATGGTTTTGCATGCCGAG‑CAGATTGAATTGATAGATTCt < 1:25321/250‑1 (MQ=255) gTCCACCAGTAATGCCTCCCTTCCAGTCCGTGTTTGAGCATCGTTTTGTGCAGGAGGAAGCCTGCTTTCACCGGTAGGCGGGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATACCGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAATAACGG‑ACTGAGCAAAAGGGTTTTGCATGCCGAG‑CAGATTGAATTGATAGATTCTg < 2:211410/249‑1 (MQ=255) gAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGG‑ACTGAGCAAAATGGTTTTGCATGCCGAG‑CAGATTGAATTGATAGATTCTGGAGACTAGcc > 1:120519/1‑250 (MQ=255) gTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGG‑ACTGAGCAAAATGGTTTTGCATGCCGAG‑CAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTc > 1:143561/1‑251 (MQ=255) tCGTTCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCAGTACTCACAGTATAACGGTCGGCAGTCGCATAACCTTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGG‑ACTGAGCAAAATGGTTTTGCATGCCGAG‑CAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTcc < 1:489242/250‑1 (MQ=255) tGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACATTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGG‑ACTGAGCAAAATGGTTTTGCATGCCGAG‑CAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGtcgtcg < 1:84525/250‑1 (MQ=255) tGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTAAGGGGTTCATTTCATGCCACAAGGCAAAGAACGG‑ACTGAGCAAAATGGTTTTGCATGCCGCG‑CAGATTGAATTGATAGATTCTGGAGACTAGGCATATGGCACGTTATTTCCGTCGTCGc > 2:368788/1‑251 (MQ=255) gtgCCGGATGAAGCCGGCTGTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGG‑ACTGAGCAAAATGGTTTTGCATGCCGAG‑CAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCCCGTTATTTCCGTCGTCGCAAGTTCTGCCGt < 2:417226/250‑1 (MQ=255) tgCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGG‑ACTGAGCAAAATGGTTTTGCATGCCGAG‑CAGATTGAATTGATAGATTCTGgag < 2:171165/204‑1 (MQ=255) tgCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGG‑ACTGAGCAAAATGGTTTTGCATGCCGAG‑CAGATTGAATTGATAGATTCTGgag > 1:171165/1‑204 (MQ=255) gCAGGAGGAAGCCGGATTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCCCAAGGCAAAGAACGG‑ACTGAGCAAACTGGTTTTGCATGCCGAG‑CAGATTGGATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCTTCGCAAGTTCTGCCGttt > 2:479440/1‑250 (MQ=255) aggaggAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGG‑ACTGAGCAAAATGGTTTTGCATGCCGAG‑CAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCAc > 1:439891/1‑251 (MQ=255) agccgctttgccccGGAAGGCGTGGTGTCAAATGCCCTTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACATTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGG‑ACTGAGCAAAATGGTTTTGCATGCCGAG‑GAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCTTCGTCGCAAGTGCTGCCGTTTCACCGCGGaa < 2:349289/239‑1 (MQ=255) cctctcccgatctaGGCGTGGTGTCAAATGCCCTTTATTTTTAGCGGAAACGAAAAACAGGCCAGTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGG‑ACTGAGCAAAATGGTTTTGCATGCCGAG‑CAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCg < 2:462789/238‑1 (MQ=255) cACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGG‑ACTGAGCAAAATGGTTTTGCATGCCGAG‑CAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAag < 1:221911/251‑1 (MQ=255) cACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGG‑ACTGAGCAAAATGGTTTTGCATGCCGAG‑CAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCATTCaa < 1:304664/250‑1 (MQ=255) cACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGACCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGG‑ACTGAGCAAAATGGTTTTGCATGCCGAG‑CAGATTGAATTGATAGATTCTGGAGACAAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGCAGGCGTTCa > 1:516108/1‑249 (MQ=255) ccGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCCCAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCCTTTCATGCCACAAGGCAAAGAACGG‑ACTGAGCAAAATGGTTTTGCATGCCGAG‑CAGATTGACTTGATATATTCTGGAGCATAGCAATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTTACCGCGGAAGGCggtctag > 2:93757/1‑243 (MQ=255) aGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGG‑ACTGAGCAAAATGGTTTTGCATGCCGAG‑CAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGACGGCg > 1:462789/1‑238 (MQ=255) ggCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGACACAAGGCAAAGAACGG‑ACTGAGCAAAATGGTTTTGCATGCCGAG‑CAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGAc > 1:505324/1‑251 (MQ=255) tggggtCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGG‑ACTGAGCACAATGGTTTTGCATGCCGAG‑CAGATTGAATTGATAGATTCTGGAGAATAACCCTATGGCCCGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGCAGGGGTTCAagggactgactat > 2:460835/5‑239 (MQ=255) tgtCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGG‑ACTGAGCAAAATGGTTTTGCATGCCGAG‑CAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATaa > 1:26749/1‑249 (MQ=255) aaTGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCAGTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGG‑ACTGAGCAAAATGGTTTTGCATGCCGGG‑CAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATc < 1:28133/251‑1 (MQ=255) cttGTTAGCGGAAACGAAAAACAGGCCATTACTAACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGG‑ACTGAGCAAAATGGTTTTGCATGCCGAG‑CAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTCCGCTg > 2:388234/2‑250 (MQ=255) gTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGG‑ACTGAGCAAAATGGTTTTGCATGCCGAG‑CAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGaaa < 1:27532/250‑1 (MQ=255) acGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGG‑ACTGAGCAAAATGGTTTTGCATGCCGAG‑CAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCAc < 1:131576/251‑1 (MQ=255) cGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGG‑ACTGAGCAAAATGGTTTTGCATGCCGAG‑AAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCAcc < 1:290861/251‑1 (MQ=255) gAAAAACAGTCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGG‑ACTGAGCAAAATGGTTTTGCATGCCGAG‑CAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCAc > 1:416865/1‑249 (MQ=255) | CGTCGCGATGATTTCGCAAACGAAACCGCTGATGATGCTGAAGCTGGGGATTCTGAAGAGTAATTTCTGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACACGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGG‑ACTGAGCAAAATGGTTTTGCATGCCGAG‑CAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACC > NC_000913/4425451‑4425940 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |