Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	696,470	C→T	noncoding (35/75 nt)	glnX ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	696,470	0	C	T	95.5%	106.2 / NA	44	noncoding (35/75 nt)	glnX	tRNA‑Gln
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (25/17);  minor base G (0/2);  total (25/19)
Fisher's exact test for biased strand distribution p-value = 1.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGT  >  NC_000913/696224‑696709                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                aGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGa                                                                                                                                                                                                                                              >  1:76231/1‑250 (MQ=255)  gCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAAt                                                                                                                                                                                                                                            <  1:357579/251‑1 (MQ=255)     tGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGATGTAGCCTTACACCATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGCCATTGAAGGATAAGACGCGTCAACATCGCATTCGACATTGAATGAAAGCAGAAAAGCAAAAAACTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCAACCCCCGGAATGCCGGAATTAGatac                                                                                                                                                                                                                                           >  2:278345/1‑246 (MQ=255)      gCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCCACATCGCATTCGACATTGAATGAACGCAGAAACGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGAGGCTGGGGTACGAGGATTCGAACCGCGGACTGCCGGACTTAGAATCCgg                                                                                                                                                                                                                                        >  2:130098/1‑251 (MQ=255)         ggCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGt                                                                                                                                                                                                                                       <  1:321362/249‑1 (MQ=255)           cGTGTTGAAAAGGCAGGGGCTGAAAAATGCGTTAGGGTTGTAGCCTTACAAAAGTGCCGGATGCGGGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATGGAATGAACGTAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATGCGAACCTCGTAATGCCGGAATTAGAAGCCGGTGcat                                                                                                                                                                                                                                   <  2:487421/251‑3 (MQ=255)                   aaCGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCtt                                                                                                                                                                                                                           <  1:76492/251‑1 (MQ=255)                     cGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGACTGCCGGAATTAGAATCCGGGTCCTTGCCGCTTgg                                                                                                                                                                                                                         >  2:229623/1‑251 (MQ=255)                          gtcgcTGAAATAGGCGGTAGGGTTGTAGCCTTCCAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGTAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATa                                                                                                                                                                                                                    <  2:133950/249‑1 (MQ=255)                                gAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCa                                                                                                                                                                                                               >  1:20386/1‑250 (MQ=255)                                 aaaTATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTACTGTGGCTGGGGGACGAGGATTCGAACCTCGGAATGCCGGAATTCGAATCCGGTGCCTTACCGCTTGGCGATACCCCAAc                                                                                                                                                                                                             >  1:34774/1‑251 (MQ=255)                                    tatGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTAGCGATACCCCAACTgg                                                                                                                                                                                                          >  1:459744/1‑251 (MQ=255)                                            aGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                                                                                                                                                                                                   <  2:432193/250‑1 (MQ=255)                                                           caacaTTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAAACCGGTGCCTTACCGCTTGGCGATACCACAACAGGAGGCAGTTACAAGGGAAGCGTCt                                                                                                                                                                                   >  1:489561/1‑251 (MQ=38)                                                             acaTTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTCCCGCTTGGCGATACCCCAACTGGATGCAATTACCAGGTAAGCGTCTTg                                                                                                                                                                                 >  1:414131/1‑251 (MQ=255)                                                              caTTGCCGGATGCGTGCCAAACGTAGGTCGGATCAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGAGTCAACATCGCATTCGACATTGAATGAACGCAGAACAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGACACTCGGCATGCCGGAATTTGAATACGGTGCCTTACCGCATGGCGATACCCCAAACGGCTGCACTTAccatgtaacatcattg                                                                                                                                                                                 >  2:325598/1‑235 (MQ=255)                                                                 tGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAAt                                                                                                                                                                              >  1:131940/1‑250 (MQ=255)                                                                   ccGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATaa                                                                                                                                                                            >  2:187979/1‑250 (MQ=255)                                                                               cAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAACGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGAACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCGGTTACAAGTTGAGCGTCTTGAATAAGTTGGCTgtgtc                                                                                                                                                                >  1:362662/1‑246 (MQ=38)                                                                                aaCCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACATGGTAAGCGTCTTGAATAAATTGGCTggagcac                                                                                                                                                              >  1:230328/1‑246 (MQ=255)                                                                                       ggTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGAtt                                                                                                                                                       <  1:160628/251‑1 (MQ=255)                                                                                        gTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTc                                                                                                                                                      <  1:187979/251‑1 (MQ=255)                                                                                               aaGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTc                                                                                                                                               <  1:165027/251‑1 (MQ=255)                                                                                                 gACGCGGCAGCGGCGCATCCGACATTGAAGGATAATAAGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTAGGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAGTGGCTGGGGTACGAGGATTCGAACCTCgg                                                                                                                                             <  2:131940/251‑1 (MQ=255)                                                                                                                cATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTTGCTGGGGTACGAGGATTCGAACCTAGGACTGCCGGaaac                                                                                                                                 >  1:125047/1‑246 (MQ=255)                                                                                                                   ccGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATc                                                                                                                           >  1:133321/1‑251 (MQ=255)                                                                                                                            aaGGATAAGAAGTGTCAACATCGCATGCGACAGTGAATGAAAGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTGTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGTATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCtt                                                                                                                  <  1:319174/251‑1 (MQ=38)                                                                                                                             aGGATAAGACTTGGCAACAGCGCAATCGACATTGAATGAACGCAGAAAAGCAAAACGCACGCCGAAGCGAGCTTTTTTAATTTGGCTGGGGTACCAGGATACGAACCTCGGAATGCGAGAATGAGAAACCGGTGCCTTACCGCTTGGCGATACCCCGACAGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGTGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTa                                                                                                                 <  2:155306/251‑1 (MQ=255)                                                                                                                                                         aCATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCAAACTGGGTGCACTTACAAGGTAAGAGCCTTGAATAACTTGGCAGGGGGACGAGGCTTCGAACCTCGGAATGCCGGCATACGAATGCGGTGCCTTACCGATTGGCGATACCTCAACAAATTGGt                                                                                     >  1:50232/1‑251 (MQ=37)                                                                                                                                                           tgtgaatggaCGCAGAAAAGGAAAAAGCTTGCCGTAGAGAGTTTGTTTAAGGTGGCTGGGGTACGAGGACTCGAGCCTCGGAATGACGGTATTTGAAACCGGTGCCTTACCGCTTGGCGATACGCCAACAGGGTGCACTTACAAGGTAAGCGGCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGtt                                                                                    <  2:396726/248‑1 (MQ=25)                                                                                                                                                                                           cGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGGACTTACAAGGTAAGAGCCTTGAATAAATTGGCTGGGGTACGAGGATTCGAAACTAGGAATGCCGGTATCAGCATCCGGTGCCTTAATGCTTGGTGATACCCTAACATATTGGTTTTGAATTTGCCGCACATATTCGATACATTc                                                      >  1:411847/1‑248 (MQ=37)                                                                                                                                                                                             aaGCGTGCTTTTATAAATTGGCGGGGGGTCGAGGGTTCGAACCAAGGAATGACAGAATTAGAATCCGGTGCCTTAGCGCTTGGATGGACCCCGACTGGGTGCAATTTCAAGGAAAGCGTCTTGCATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAAt                                                 <  2:380758/251‑1 (MQ=21)                                                                                                                                                                                               gCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTATAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACACGGTAAGCGTCTTGAATAAATTGGCTGGGGCACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATACGATACATTCAGAAttt                                               >  1:482591/1‑251 (MQ=255)                                                                                                                                                                                                 gAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGCTTCGAACCTCGGACTGCCGGAATCAGAATCCGGTTCCTTACCGCTTGGCGATACCCCAACAAATTGGCTTTGCATTTTCCGCACATATTCGATACCTTCAGAATTtgg                                             >  1:310832/1‑251 (MQ=38)                                                                                                                                                                                                                       gTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGACCATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAAcc                       >  1:274951/1‑251 (MQ=255)                                                                                                                                                                                                                       gTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGAACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGCATTTGCCGAAc                                                                     >  1:193904/1‑205 (MQ=255)                                                                                                                                                                                                                         aCGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTTGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCAGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTTCCGAACATATTCGAAACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCtg                     >  1:450320/1‑251 (MQ=255)                                                                                                                                                                                                                          cGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCtgt                    <  1:333823/251‑1 (MQ=255)                                                                                                                                                                                                                           gaggatgcgctccttggAATGGCGGAATTAGAAACCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCATTTACAAGGGAAGCGTCTTGAATAAATTGGCTGGGGTTCGAGGATTCGAACCTCTGAATGCCGGAATCAGAATCCAGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGACCCtgtg                   <  2:450320/236‑1 (MQ=255)                                                                                                                                                                                                                                 tCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGAccccc             >  1:161378/1‑250 (MQ=255)                                                                                                                                                                                                                                 tCGAACCTCGGAAGGCCGGAAGTTGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGAcccc              <  2:183103/250‑1 (MQ=255)                                                                                                                                                                                                                                 ccGAACCTTGGAATGCCGGAATTAGAATCCGGTGCCTTACCGATTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGGCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCca             <  2:9465/250‑1 (MQ=255)                                                                                                                                                                                                                                         cGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGTCGACCGTAGTCGATACATTCAGACTTTGGTGGCTACGTCGGGATACGAGCCTGTGACCCCCTCAttctg     >  1:325362/1‑248 (MQ=37)                                                                                                                                                                                                                                            aaTGCCGGAATGAGAACCCTGTGCCTTACCGCTTGGCTATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGt  <  1:377576/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                AGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGT  >  NC_000913/696224‑696709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑