Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,223,638	T→C	intergenic (‑10/‑190)	iclR ← / → metH	DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,223,638	0	T	C	100.0%	129.5 / NA	37	intergenic (‑10/‑190)	iclR/metH	DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/29);  total (8/29)

AGTTCGCCAACCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCT  >  NC_000913/4223393‑4223883                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      aGTTCGCCAACCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCcttttt                                                                                                                                                                                                                                                  <  1:136235/251‑1 (MQ=255) aGTTCGCCAACCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCcttttt                                                                                                                                                                                                                                                  <  1:351057/251‑1 (MQ=255) aGTTCGCCAACCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCcttttt                                                                                                                                                                                                                                                  <  2:185908/251‑1 (MQ=255)   gtCGCCAACCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTACGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCt                                                                                                                                                                                                                                                <  1:70122/250‑1 (MQ=255)          aCCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGGGGCAACGGCGGGGTTTTTT‑CCCCGTTTCGCGGGGATTGGAGGGGCCATGACAGCCTCctttttttttttctt                                                                                                                                                                                                                                         >  1:217457/1‑242 (MQ=255)              gccGCACGAAACCCGGCTGTTGCATCGTGGTGAGCAGGGGGTTGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATTCACTCCAGTAATTTCAGGGCACGCGTTAAAGACTTAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGTGTTTTCTG‑CCGCGTGTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGaa                                                                                                                                                                                                                                     <  2:283285/249‑1 (MQ=255)              gACGCACGAAACCCGGCTGTTGCATCGTGGTTAGCCGGCGGTGGGTCGTGGAATTTGGGAACCCGGCTTGTTGCGCCAGTTCCGTGAGTTCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGaa                                                                                                                                                                                                                                     <  2:440999/251‑1 (MQ=255)                     gAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAAtcattt                                                                                                                                                                                                                              <  1:72274/251‑1 (MQ=255)                        aCCCTGCTGTTCCTTCGTGGTTTGCAGGCTGTGGTTCGCGGAATCGGGTAACCCCGCTTTTTGCCCCCGTTCCGTGAGTGCCCCACTGCCTTTGGATTCGGCAGTCCACGCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGTTGCGACCATGACAGTCTCCTTTTTCTGTTTCGTGGAAAtcattttca                                                                                                                                                                                                                           <  2:509373/251‑1 (MQ=255)                         cccttcttttGCATCGTTGTTAGACGGCGGTGGTGGGTGGAATTGGGTAACCCTGTTTGTTGCGCCAGTTCCTTGAGTGCCTCTCTGCCATTTGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAATTCTGAGCCTGTCCAGTCGCTGGTGCGGTGGTAACGGCTGGTTTTCTG‑CCGCGTTTCGCGTGAATGGGTTCGTCCATGACCGTCTTCTTTTTCTTTATCGTGGAAAtcatgttcat                                                                                                                                                                                                                          <  2:350511/243‑1 (MQ=255)                           ctgctgTTGCATCGCGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTCTGTCCG‑GGTTCGCGGGGATGGGGGCGGCCATGAAAGTCTCCTTTTTTTGTATCGTGGGAAtcctttttcttt                                                                                                                                                                                                                        >  1:406367/1‑246 (MQ=255)                                gTTGCCTCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTAt                                                                                                                                                                                                                    <  2:387996/250‑1 (MQ=255)                                   gaaTCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAAACCGTCTTGTTGCGCCAGTTTCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGGTGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGt                                                                                                                                                                                                                 <  1:368019/248‑1 (MQ=255)                                       cGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGc                                                                                                                                                                                                             <  1:144434/250‑1 (MQ=255)                                          ggTTAGCAGGCGGTGGGTCGTGGAATTGTGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTaa                                                                                                                                                                                                          <  1:353878/250‑1 (MQ=255)                                                   gCGGTGGGTCGTGGAATTGGGTATCCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGTGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGtt                                                                                                                                                                                                <  1:464783/251‑1 (MQ=255)                                                        gggTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTactga                                                                                                                                                                                           <  1:88638/251‑1 (MQ=255)                                                                            ccGGCTTGTGGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATg                                                                                                                                                                       <  2:253710/251‑1 (MQ=255)                                                                                       cgcCCGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCATGCGTTAAAGACTGATCCTGTCCAGTCGCTGGGGCGGTGGCAACGGCGGGTTGTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTGTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAAtc                                                                                                                                                            <  2:42351/251‑1 (MQ=255)                                                                                                cGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGGTAATGTTGAACAAATCTCATGTTGc                                                                                                                                                   <  1:443277/251‑1 (MQ=255)                                                                                                 gTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTG‑CCGCGGTTCGCGGGGATGGGGGCGACCATGACAGGCTCCTTTTTTTGTATCGTGGGAATCATTTTCATTTTTTTTTTTTGCTTATGCAATAGTTACTGACCTGATCCGATTAGTTAATGTTGTACAAATCTCCTGtttcg                                                                                                                                                  >  1:471788/1‑248 (MQ=255)                                                                                                          acccTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCg                                                                                                                                         <  2:105297/248‑1 (MQ=255)                                                                                                            acTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCt                                                                                                                                       <  1:536381/251‑1 (MQ=255)                                                                                                               gCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTGTCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCttt                                                                                                                                     <  1:60355/250‑1 (MQ=255)                                                                                                               gCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCtt                                                                                                                                      <  1:258216/249‑1 (MQ=255)                                                                                                                       aTTCGGCAATCCACTTCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGa                                                                                                                            <  2:498282/251‑1 (MQ=255)                                                                                                                                   aCTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCCCGGTTCTCGGGGATGGGGGCGACCATGACAGACTCCTTTTTTTTTATTGTGGGAATCCTTTTCATTTTTTTTTTTATCTATTGCCATAGTTAATGAACTGATTCGATGAGTTAATGTTGAACAAATATCAAGTTGCGTGGTGGGCGGTTTTACCCCAGATGCGTTTTTGcc                                                                                                                >  1:505780/1‑251 (MQ=255)                                                                                                                                      ccAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAg                                                                                                              <  1:161269/250‑1 (MQ=255)                                                                                                                                                                gACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTT‑CCGCGTTTCGCGGGGAAGGGTGCGGCCATGACAGGCTCCCTTTTTTGTATCGTGGAAATTATTTTTATTTTTATTTTTAGCTAATGCCATAATTATTGAAATGATCCGGTGAGTTTATGTTGAACAAATCTCATGTTGCGTGGTGGGCGCTTTTACCACAGATGCGTTTATGCCAGGATGGGTTGTTGAATTTTTATTAAATc                                                                                   >  1:304756/1‑251 (MQ=255)                                                                                                                                                                 aCTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATTGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTTGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCt                                                                                  <  1:120570/251‑1 (MQ=255)                                                                                                                                                                            ccAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTCTG‑CCGCGTTTCGCGGGGATGGGGGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTTTTGTTAGCTAATGCAATAGTTACTGAAATGATCCGATGAGTTAATGTTGAAAAAATCTCATGTTGCGTGGTGGTCGCTTTTTCCACAGATGCGTTTATTCCAGTATGGTTTGTTGAATTTTTTTTAAATTTGGGTTGAGCgg                                                                       >  1:383263/1‑250 (MQ=255)                                                                                                                                                                             cAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACCGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCgtg                                                                      <  1:151635/251‑1 (MQ=255)                                                                                                                                                                                     ggTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAg                                                               <  1:273951/250‑1 (MQ=255)                                                                                                                                                                                               cAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGagc                                                    <  1:356362/251‑1 (MQ=255)                                                                                                                                                                                                      gggTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTGTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAg                                             <  2:92244/251‑1 (MQ=255)                                                                                                                                                                                                           ttCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAg                                                                                                              <  1:331152/181‑1 (MQ=255)                                                                                                                                                                                                           ttCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGACAGCCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAg                                                                                                              >  2:331152/1‑181 (MQ=255)                                                                                                                                                                                                                       ttCGCGGGAATGGGTTCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGGTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTAGGCCAGTATGGTTTGTTGAAGTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACtgcgtg                              <  2:217457/250‑1 (MQ=255)                                                                                                                                                                                                                                aTGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTaa                     >  1:132129/1‑250 (MQ=255)                                                                                                                                                                                                                                    gcgCGACCATGACAGTCTCCTTTTCCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCACTAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATTTCATGTTGCGTGGTGGACGCTTTTACCACAGATGCGTTTATGCCAGCATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGACGGGAGCAAGAGTGAGCAGCAAAGTGGACCAACTGCGTGCGCAGTTAACTGa                 >  1:94340/3‑250 (MQ=255)                                                                                                                                                                                                                                                  gTCTCCTTTTTCGGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCt  <  1:64923/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      AGTTCGCCAACCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCT  >  NC_000913/4223393‑4223883

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑