| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,171,788 | G→T | intergenic (+32/‑269) | rrfB → / → murB | 5S ribosomal RNA/UDP‑N‑acetylenolpyruvoylglucosamine reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,171,788 | 0 | G | T | 92.9% | 37.3 / ‑0.8 | 29 | intergenic (+32/‑269) | rrfB/murB | 5S ribosomal RNA/UDP‑N‑acetylenolpyruvoylglucosamine reductase |
| Reads supporting (aligned to +/- strand): ref base G (0/2); new base T (11/15); total (12/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.77e-01 | |||||||||||
TTACAACGCCGAAGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAG‑TCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCAT > NC_000913/4171543‑4172005 | ttACAACGCCGAAGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAG‑TCGAAAGACTTGGCCt > 1:277574/1‑251 (MQ=37) tACAACGCCGAAGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCTGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCAGGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAG‑TCGAAAGACTTGGcc < 1:523448/249‑1 (MQ=255) aCGCCGAAGCTGTTTGGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAAGCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGTGCCCACCTGCCCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGTGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAG‑TCGAAAGACTTGGCCTTTCGt < 2:26391/251‑1 (MQ=11) cGCCGAAGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAG‑TCGAAAGACTGGGCCTTTCGtt < 1:193163/251‑1 (MQ=37) ttgtGGCTGATGAGAGAATAGTTGCAGCCTGATACAGATTAAATCAGAACGCAGTAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCTGTGGTCCCACCTGACCCCAGTCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCTAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAG‑TCGAAAGACTTGTCCTTTCGTTTTATCtgttg < 2:502060/247‑1 (MQ=11) gaagaTTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAG‑TCGAAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGct < 1:477761/251‑1 (MQ=18) cAGCCTGATAATGATTAAATCAGAACGCAGAAGCGGACTGATAAAACAGAATATGCCTGGCGGCAGCAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCCGAAGTGAAACGCCGTAGAGCCGGTGGTAGTGTGGGGCCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATCAAACGAAAGGCTCAG‑ACGAAAGACTCGACCTTTCGTTTTATCTTTTGGTTTTCGGTGAACGCTCTCCTGAgg > 2:485246/1‑250 (MQ=11) tGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCTAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAG‑TCGAAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGGAGGAc > 1:174303/1‑251 (MQ=18) aTACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAG‑TCGAAAGACTTGGCCTTTCGGTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACaa < 1:181080/251‑1 (MQ=18) agctttaaTCAGAGCGCAGAAGCGGTCTGATAAAACAGAGTTGGCCAGGCAGAAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGACCTCAGAAGGGGAACGACGTAGCGACGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGCCATCCAATAAAACGACAGGATCAG‑TCGCCAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGGGTAGGAAAAATc > 2:41389/7‑250 (MQ=11) tAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAG‑TCGAAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATccgcc < 1:504815/250‑1 (MQ=18) aGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCAGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAG‑TCGAAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCg > 1:499986/1‑251 (MQ=18) aaGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAG‑TCGAAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCGCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTg < 2:355167/250‑1 (MQ=18) aaGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGAGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAG‑TCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAAc < 1:523483/251‑1 (MQ=18) ggTCTGATAAAACAGAATTTGCCTGGCGGCAGGAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAG‑TCGAAAGACTTGGCCTTTCGTTTTAGCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTg < 2:345617/251‑1 (MQ=18) aaaCAGAAGTGGCCCGGCGGCAGTGGCGCGGTGGTCCCACCTGACCCCAGGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAGCGAAAGGCTCAG‑TCGAAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAAc < 2:19127/251‑1 (MQ=11) ggcggcAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAG‑TCGAAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTgg < 1:137221/250‑1 (MQ=18) cggcAGTAGCGCGGTGGTCCCACCTGACACCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAG‑TCGAAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGATGGTGGCgg > 1:160439/1‑251 (MQ=18) ccGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAG‑TCGAAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAgg > 1:141158/1‑160 (MQ=25) ccGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAG‑TCGAAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAgg < 2:141158/160‑1 (MQ=25) gAACTCAGAAGTGAAACTCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAG‑TCGAAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGAGGATTTGAACGTTGCGAAGCAACGGCCCAGGGGGTGGCGGGCAGGCCGCCCGCCCTAAACTGcccggactcacatt > 2:82356/1‑239 (MQ=11) ccgcggTAGCGACGTTGGTGGTGTGGGGTCTCCCCATGGGAGAGGAGGAAACTGCCAGGAATAAAATAAAACGAAAGGCTCAG‑TCGAAAGGTTTGGCTTTTGTTTTTATCTTTTTTTTGGCGGTGACCGCTCTCTTGATGAGAAAAAACCCGCCGGGGGCGGATTTGAATGTTGCGAAGCAACGGCCGGGGGGGTGGCGGGCAGGAGGGCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGTCCATc < 1:446739/246‑1 (MQ=2) gttgcccctgATGGGAGTGGGGGGTTTCCCTAGGCGAGAGGGGGGAACTGCCAGGCATAAAAAAAAACGAACGGC‑CAGGTTAAAAGACTTGTCCTCTTGTTTTCTTTGTTGTTTGGGTGTAAACGCCCTCGTGCTTAGGAAAACTCGGCTGGGTGCGGATTTGCCCGTGGCTAACCAACGGCCGGGAGGGGGGGGGGAAGCACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGa < 1:275/242‑1 (MQ=2) cGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAG‑TCGAAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCACCCAAAACTGCCAGGGCTCAAATTAAGGAGAAGGCCATccgac > 2:169746/1‑248 (MQ=11) gggcCGAGGGTAGTGGGGGTTCTCCCCAGGCGAGAGTAGGGACCGGCCAGGAATAAAAAAAAACGAAAGGCTCAG‑TCGAAAGTCTTGGCTTTTCTTTTTTTCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAACTCCGCCGGGGGCGTATTTGAGCGTTGCGAAGAAAGGGCCGGGGGGGTGGGGGGAAGGCCGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGa < 1:482883/249‑1 (MQ=2) gTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAG‑TCGAAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCtttt > 1:485631/1‑251 (MQ=18) ctcCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAG‑TCGAAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAg > 1:241412/1‑210 (MQ=18) aacagaTAAAACGAAAGGCTCAG‑TCGAAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAgg < 1:275056/140‑1 (MQ=12) aTCAAATAAAACGAAAGGCTCAG‑TCGCAAGACTTGGCCTTTCGATTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACACCCGCCATACACTGCCAGGCCTCCAATTAAGCCGAAGGCCCCCCTGACGGATGGCCTTTTTGCGATTATACCAACTCTTCCTGTAGTCATATCTCCAAGCCAt > 2:56731/1‑251 (MQ=14) | TTACAACGCCGAAGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAG‑TCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCAT > NC_000913/4171543‑4172005 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |