Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	418,156	C→T	R90C (CGC→TGC)	phoR →	sensor histidine kinase PhoR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	418,156	0	C	T	98.1%	143.0 / NA	55	R90C (CGC→TGC)	phoR	sensor histidine kinase PhoR
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (25/27);  minor base G (0/1);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCTACTTTGCTGCCTCCCGGCTTTCATCCTGGGTGCATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCCGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCTGACCACGGAAGAGGGCGGTATTTTCTGGTGTAACGGTCTGGCGCAACAAATTCTTGGTTTGCGCTGGCCGGAAGATAACGGGCAGAACATCCTTAACCTACTGCGTTACCCGGAGTTTACGCAATATCTGAAAACGCGTGATTTTTCTCGCCCGCTCAATCTGGTGCTCAAC  >  NC_000913/417929‑418398                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   tgctACTTTGCTGCCTCCCGGCTTTCATCCTGGGTGCATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCTGCCGTGAACTGGGCAATCTGAtt                                                                                                                                                                                                                             >  1:191852/1‑251 (MQ=255)        ttGCTGCCTCCCGGCTTTCATCCTGGGTGCATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCATCGGTAACAGGCCTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCTGCCGTGAACTGGGCAATCTGATTAAACg                                                                                                                                                                                                                        <  1:94921/249‑1 (MQ=255)              ccTCCCGGCTTTCATCCTGGGTGCATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCTGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGt                                                                                                                                                                                                                 >  1:497127/1‑250 (MQ=255)                  ccGGCTTTCATCCTGGGTGCATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCTGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAgcgg                                                                                                                                                                                                            <  1:214580/251‑1 (MQ=255)                             ccTGGGTGCATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCCGCTGCAGCTGCGAAATCAAAAACGCCGCCGTGAACTGGGCAATCTGCTTAAACGCTTTCGTAGCGGCGCGGAGTCGc                                                                                                                                                                                                 >  2:454319/1‑251 (MQ=255)                             ccTGGGAGCCTTTTTTGGTTACATGCCATGGTTATTACTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCAAGGTGGCTGTGTGTGGATCGCAGCATGAACCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTAATATACGGCATACCACATAAGGCGCTGCTAAATAAAAAAAGCAGCCGGGCCCTGGGCAATCGGAGTACAAGCTTTCATAgcggagaggtgtagg                                                                                                                                                                                                 >  2:284083/1‑240 (MQ=255)                              cTGGGTGCATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGGAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCTGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGCCGCGGAgcagct                                                                                                                                                                                                >  1:231704/1‑246 (MQ=255)                                    gctttttgtGGTTACCTGCCCTGGTTTTTGCCGGCATCGGTAACAGGACTGCTTACCTGGCATTGCTGGAATTTATTGCGCCTTTCAGGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCGGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCTGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCg                                                                                                                                                                                           <  2:167600/247‑1 (MQ=255)                                    gCATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCTGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCgc                                                                                                                                                                                          >  1:82865/1‑250 (MQ=255)                                     cATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCTGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGAc                                                                                                                                                                                         <  2:308217/251‑1 (MQ=255)                                                          gtttttgGCTGGCATCGTTAAAAGGACTTCTTATCGGGCATGTTTGGAATTTCTGGCGCCCTTCATGGTGGCTGTGGGGTGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTCCACCAGATGCAGCTGCGAAATAAAAAACGCTGCCGTTAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCTGACCACGGaa                                                                                                                                                                    <  2:414977/249‑1 (MQ=255)                                                                    ggCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCTGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCTGACCACGGAAGAGGGCgg                                                                                                                                                            <  1:252246/249‑1 (MQ=255)                                                                       aTCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCCGCTGCGAAATAAAAAACGCTGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGAGCGGAGTAGCTGCCCGACGCGGTGGTGCGTACCACGGGAGAGGGCGGTAttt                                                                                                                                                       >  2:501335/1‑251 (MQ=255)                                                                         cGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCTGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCTGACCACGGAAGAGGGCGGTAtttt                                                                                                                                                      <  1:518314/250‑1 (MQ=255)                                                                          ggTAACAGGACTGCTGATCTGGAATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGGGGATCGCAGTATGACACCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCTGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCCGCGCGGAGTCGCTGCCCTACGCGGTGGTGCTGACCACGGAAGAGGGCGGTATTTTCt                                                                                                                                                    <  1:312385/251‑1 (MQ=255)                                                                            tAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCTGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCTAACCCCGGAAGAGGGCGGTATTTTCTgg                                                                                                                                                  >  2:366814/1‑251 (MQ=255)                                                                                              ggCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCTGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCTGACCACGGAAGAGGGCGGTATTTTCTGGTGTAACGGTCTGgcg                                                                                                                                   <  1:222258/248‑1 (MQ=255)                                                                                                    tCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCTGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGGGGTGCTGACCACGGAAGAGGGCGGTATTTTCTGGTGTAACGGTCCGGCGCAACAAAtt                                                                                                                          >  2:207190/1‑251 (MQ=255)                                                                                                        gAATTTATTGTGCCTTTCATGGTGGTGGTGGGTGGATCGCAGTATGACCCCGCCCCCGGGGCTTGGTAGTTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCTGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCTGACCACGGAAGAGGGCGGTATTTTCTGGTGTAACGGTCTGGCGCAACAAATTCTTg                                                                                                                      <  2:532239/251‑1 (MQ=255)                                                                                                         aaTTTATTGCGCATTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCTGCCGTGACCTGGGCAATCTGATTAAACGCTTTCGTAGCAGCGCGGAGACGCTGCCCGACGCGGTGGTGCTGACCACGGAAGGGGGCGGTATTTTCTGGTGGTACGGTCTGGCGCAACAAATTCTTgg                                                                                                                     >  2:395675/1‑251 (MQ=255)                                                                                                            ttATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCTGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCTGACCACGGAAGAGGGCGGTATTTTCTGGTGTAACGGTCTGGCGCAACAACTTCTTGGttt                                                                                                                  >  2:95031/1‑251 (MQ=255)                                                                                                             tATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCTGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCTGACCACGGAAGAGGGCGGTATTTTCTGGTGTAACGGTCTGGCGCAACAAATTCTTGGTTTg                                                                                                                 >  1:105097/1‑251 (MQ=255)                                                                                                              aTTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCTGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCTGACCACGGAAGAGGGCGGTATTTTCTGGTGTAACGGTCTGGCGCAACAAATTCTTGGTTTgc                                                                                                                >  2:46029/1‑251 (MQ=255)                                                                                                                  cgcCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCTGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCTGACCACGGAAGAGGGCGGTATTTTCTGGTGTAACGGTCTGGCGCAACAAATTCTTGGTTTGCGCTg                                                                                                            >  1:8842/1‑251 (MQ=255)                                                                                                                     cTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCTGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCTGACCACGGAAGAGGGCGGGATTTTCTGGTGTAACGGTCTGGCGCAACAAATTCTTGGTTTGCGCTGGcc                                                                                                         >  2:138325/1‑251 (MQ=255)                                                                                                                       ttCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCTGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCTGACCACGGAAGAGGGCGGTATTTTCTGGTGTAACGGTCTGGCGCAACAAATTCTTGGTTTGCGCTGGcc                                                                                                         <  1:12204/249‑1 (MQ=255)                                                                                                                                gCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGTGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCTGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCTGACCACGGAAGAGGGCGGTATTTTCTGGTGTa                                                                                                                                              >  1:454320/1‑203 (MQ=255)                                                                                                                                gCTGGGGGTGGATCGCAGTATGACCCCGCCACCGGTGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCTGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCTGACCACGGAAGAGGGCGGTATTTTCTGGTGTa                                                                                                                                              <  2:454320/203‑1 (MQ=255)                                                                                                                                  gtgggtgggaccgcatgaTGACCCCGCCCCCGGGGCGTGGTAGTGGGGAACCGCTACTATACGGCTTGCTGCAGATGCAGCTGCGAAATAAAAAACGCTGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCTGACCACGGAAGAGGGCGGTATTTTCTGGTGTAACGGTCTGGCGCAACAAATTCTTGGTTTGCGCTGGCCGGAAGATAACggg                                                                                            <  2:414648/234‑1 (MQ=255)                                                                                                                                   gtggggtgATCGCAGTATGACCCCGCCGCCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCTGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCTGACCACGGAAGAGGGCGGTATTTTCTGGTGTAACGGTCTGGCGCAACAAATTCTTGGTTTGCGCTGGCCGGAAGATAACggg                                                                                            <  1:324046/243‑1 (MQ=255)                                                                                                                                    tgggtggATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTCTACGGCTTACACCAGATGCAGCTGCGCAATAAAAAACGCTGCCGTGAACTGGGCAAACTGATTAAACGCTTTCGTAGCGGCGCGGAGTAGCTGCCCGACGCGGTGGGGCTGGGCACGGAAGAGGGCGGTATTTTCTGGTGTAACGGGCTGGCGCAACAAATTCTTGGGTTGCGCTGGCCGGATGATAACGGGc                                                                                           >  1:183648/1‑250 (MQ=255)                                                                                                                                          gATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCTGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCTGACCACGGAAGAGGGCGGTATTTTCTGGTGTAACGGTCTGGCGCAACAAATTCTTGGTTTGCGCTGGCCGGAAGCTAACGGGCCGAACAt                                                                                    >  2:199397/1‑251 (MQ=255)                                                                                                                                           aTCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCTGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCTGACCACGGAAGAGGGCGGTATTTTCTGGTGTAACGGTCTGGCGCAACAAATTCTTGGTTTGCGCTGGCCGGAAGATAACGGGCAGAACATc                                                                                   >  1:89661/1‑251 (MQ=255)                                                                                                                                                        cccGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCTGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCTGACCACGGAAGAGGGCGGTATTTTCTGGTGTAACGGTCTGGCGCAACAAATTCTTGGTTTGCGCTGGCCGGAAGATAACGGGCAGAACATCCTTAACCTACTGc                                                                      <  1:202268/251‑1 (MQ=255)                                                                                                                                                                   ggCGTGTTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCTGCCGTGAACTGGGCAATCTGAGTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCTGACCACGGAAGAGGGCGGTATTTTCTGGTGTAACGGTCTGGCGCAACAAATTCTTGGTTTGCGCTGGCCGGAAGATAACGGGCAGAACATCCTTAACCTACTGCGTTACCCGGAg                                                           <  1:538636/251‑1 (MQ=255)                                                                                                                                                                   ggCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCTGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCTGACCACGGAAGAGGGCGGTATTTTCTGGTGTAACGGTCTGGCGCAACAAATTCTTGGTTTGCGCTGGCCGGAAGATAACGGGCAGAACATCCTTAACCTACTGCGTTACCCGGAg                                                           <  1:307327/251‑1 (MQ=255)                                                                                                                                                                    gCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCTGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCTGACCACGGCAGAGGGCGGTATTTTCTGGTGTAACGGTCTGGCGCAACAAATTCTTGGTTTGCGCTGGCCGGAAGATAACGGGCAGAACATCCTTAACCTACTGCGTTACCCGGAGt                                                          >  1:433397/1‑251 (MQ=255)                                                                                                                                                                       gggTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCTGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCTGACCACGGAAGAGGGCGGTATTTTCTGGTGTAACGGTCTGGCGCAACAAATTCTTGGTTTGCGCTGGCCGGAAGATAACGGGCAGAACATCCTTAACCTACTGCGTTACCCGGAGTTTa                                                       <  1:225500/250‑1 (MQ=255)                                                                                                                                                                               gggAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCTGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCTGACCACGGAAGAGGGCGGTATTTTCTGGTGTAACGGTCTGGCGCAACAAATTCTTGGTTTGCGCTGGCCGGAAGATAACGGGCAGAACATCCTTAACCTACTGCGTTACCCGGAGTTTACGCAat                                                 <  1:164083/249‑1 (MQ=255)                                                                                                                                                                                    ccGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCTGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCTGACCACGGAAGAGGGCGGTATTTTCTGGTGTAACGGTCTGGCGCAACAAATTCTTGGTTTGCGCTGGCCGGAAGATAACGGGCAGAACATCCTTAACCTACTGCGTTACCCGGAGTTTACGCAATATCTGaa                                          <  2:105097/251‑1 (MQ=255)                                                                                                                                                                                      gCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCTGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCTGACCACGGAAGAGGGCGGTATTTTCTGGTGTAACGGTCTGGCGCAACAAATTCTTGGTTTGCGCTGGCCGGAAGATAACGGGCAGAACATCCTTAACCTACTGCGTTACCCGGAGTTTACGCAATATCTGaaa                                         >  1:302443/1‑250 (MQ=255)                                                                                                                                                                                        tactaTACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCGGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCTGACCACGGAAGAGGGCGGTATTTTCTGGTGTAACGGTCTGGCGCAACAAATTCTTGGTTTGCGCTGGCCGGAAGATAACGGGCAGAACATCCTTAACCTACTGCGTTACCCGGAGTTTACGCAATATCTGAAAAc                                       <  1:520308/250‑1 (MQ=255)                                                                                                                                                                                         actttaCGGCTTAAACCAGATGCAGCTGCGAAATAAAAAACGCTTCCGTGAACTGGGCAATCTGATTAAACGCGTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCTGACCACGGAAGAGGGCGGTATTTTCTGGTGTAACGGTCTGGCGCAACAAATTCTTGGTTTGCGCTGGCCGGAAGATAACGGGCAGAACATACTTAACCTACTGCGTTACCCTGAGTTGACGCAATATCTGAAAAcg                                      <  2:328896/246‑1 (MQ=255)                                                                                                                                                                                         actaTACGTCTTACAACAGATGCAGCTGCGAAATAAAAAACGCTGCCGTGAACTGGGCAATCTGATTAAACGCTTCCGTAGCGGCGCGGAGTCTCTGCCCGACGCGGTGGTGCTGACCACGGAAGAGGGCGGGATTTTCTGGTGTAACGGTCTGGCGCAACAAATTCTTGGTTTGCGCTGGCCGGAAGATAACGGGCAGAACATCCTTAACCTACTGCGTTACCCGGAGTTTACGCAATATCTGAAAAcg                                      <  1:519034/250‑1 (MQ=255)                                                                                                                                                                                            ataCGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCTGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCTGACCACGGAAGAGGGCGGTATTTTCTGGTGTAACGGTCTGGCGCAACAAATTCTTGGTTTGCGCTGGCCg                                                                                                        >  2:405115/1‑181 (MQ=255)                                                                                                                                                                                            ataCGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCTGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCTGACCACGGAAGAGGGCGGTATTTTCTGGTGTAACGGTCTGGCGCAACAAATTCTTGGTTTGCGCTGGCCg                                                                                                        <  1:405115/181‑1 (MQ=255)                                                                                                                                                                                               cGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCTGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGAGGTGCTGACCACGGAAGAGGGCGGTATTTTCTGGTGTAACGGTCTAGCGCAACAAATTCTTGGTTTGCGCTGGCCGGAAGATAACGGGCAGAACATCCTTAACCTACTGCGTTACCCGGAGGTTACGCAATATCTGACAACGCGTGAtt                               >  1:275589/1‑251 (MQ=255)                                                                                                                                                                                                ggCTTACACCAGATGCAGCTGCGAAATAAAAAACGCTGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCTGACCACGGCAGAGGGCGGTATTTTCTGGTGTAACGGTCTGGCGCAACAAATTCTTGGTTTGCGCTGGCCGGAAGATAACGGGCAGACCATCCTTAACCTACTGCGTTACCCGGGGTTTACGCAATATCTGAAAAcgcgggcttt                              >  2:85817/1‑245 (MQ=255)                                                                                                                                                                                                         cAGATGCAGCTGCGAAATAAAAAACGCTGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCTGACCACGGAAGAGGGCGGTATTTTCTGGTGTAACGGTCTGGCGCAACAAATTCTTGGTTTGCGCTGGCCGGAAGATAACGGGCAGAACATCCTTAACCTACTGCGTTACCCGGAGTTTACGCAATATCTGAAAACGCGTGATTTTTCTCGccc                     <  1:366814/251‑1 (MQ=255)                                                                                                                                                                                                           gATGCAGCTGCGTAATATAAAACGCTGCCTTGTACTGTGCAATCTGATTAAACGATTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGATGGTGCTGACCACGGAAGAGGGCGGTATTTTCTGGTGTAACGGACTGGCGCAACAAATTCTTGGTTTGCGCTGGCCGGAAGATAACGGACAGAACATCCCTAACATACTTCTTTACCAGGAGTTTACGCAATCTCTAAAAAAGCGTGCTTTTTCTCTCCCGc                   >  2:360815/1‑251 (MQ=255)                                                                                                                                                                                                              gCAGCTGCGAAATAAAAAACGCTGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCTGACCACGGAAGAGGGCGGTATTTTCTGGTGTAACGGTCTGGCGCAACAAATTCTTGGTTTGCGCTGGCCGGAAGATAACGGGCAGAACATCCTTAACCTACTGCGTTACCCGGAGTTTACGCAATATCTGAAAACGCGTGATTTTTCTCGCCCGCTc                 <  2:82865/250‑1 (MQ=255)                                                                                                                                                                                                                 gCTGCGAAATAAAAAACGCTGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCTGACCACGGAAGAGGGCGGTATTTTCTGGTGTAACGGTCTGGCGCAACAAATTCTTGGTTTGCGCGGGCCGGAAGATAACGGGCAGAACATCCTTAACCTACTGCGTTACCCGGAGTTTACGCAATATCTGAAAACGCGTGATTTTTCTCGCCCGCTCAATc             <  2:497127/251‑1 (MQ=255)                                                                                                                                                                                                                     cGAAATAAAAAACGCTGCCGTTAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCGGACCACGGAAGAGGGCGGTATTTTCTGGTGTAACGGTCTGGCGCAACAAATTCTTGGTTTGCGCTGGCCGGAAGATAACGGGCAGAACATCCTTAACCTACTGCGTTACCCGGAGTTTACGCAATATCTGAAAACGCGTGATGTTTCTCGCCCGCTCAATCTGGt         <  2:315238/251‑1 (MQ=255)                                                                                                                                                                                                                            aaaaaCGCTGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCTGACCACGGAAGAGGGCGGTATTTTCTGGTGTAACGGTCTGGCGCAACAAATTCTTGGTTTGCGCTGGCCGGAAGATAACGGGCAGAACATCCTTAACCTACTGCGTTACCCGGAGTTTACGCAATATCTGAAAACGCGTGATTTTTCTCGCCCGCTCAATCTGGTGCTCAac  >  1:276338/1‑251 (MQ=255)                                                                                                                                                                                                                             aaaaCGCTGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCAGCGCGGAGTCGCTGCCCGACGCGGTGGTGCTGACCACGGAAGAGGGCGGTATTTTCTGGTGTAACGGTCTGGCGCAACAAATTCTTGGTTTGCGCTGGCCGGAAGATAACGGGCAGAACATCCTTAACATACTGCGTTACCCGGAGTTTACGCAATATCTGAAAACGCGTGATTTTTCTCGCCCGCTCAATCTGGTGCTCAac  >  1:70353/1‑250 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   TGCTACTTTGCTGCCTCCCGGCTTTCATCCTGGGTGCATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCCGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCTGACCACGGAAGAGGGCGGTATTTTCTGGTGTAACGGTCTGGCGCAACAAATTCTTGGTTTGCGCTGGCCGGAAGATAACGGGCAGAACATCCTTAACCTACTGCGTTACCCGGAGTTTACGCAATATCTGAAAACGCGTGATTTTTCTCGCCCGCTCAATCTGGTGCTCAAC  >  NC_000913/417929‑418398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑