Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,926,772	C→A	G155G (GGC→GGA)	ppnN →	nucleotide 5'‑monophosphate nucleosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,926,772	0	C	A	100.0%	164.6 / NA	62	G155G (GGC→GGA)	ppnN	nucleotide 5'‑monophosphate nucleosidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (30/32);  total (30/32)

GGAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGC  >  NC_000913/2926529‑2927013                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  ggAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACAGTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCa                                                                                                                                                                                                                                              <  1:229837/249‑1 (MQ=255)     cTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGGCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAAt                                                                                                                                                                                                                                        >  1:528022/1‑251 (MQ=255)       gATTAATCCCCCGGTAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAAtt                                                                                                                                                                                                                                       <  1:525927/250‑1 (MQ=255)       gATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAAt                                                                                                                                                                                                                                        <  1:117554/249‑1 (MQ=255)       gATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAAt                                                                                                                                                                                                                                        <  2:116284/249‑1 (MQ=255)               ccccGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAAGTAACGAAAAc                                                                                                                                                                                                                              <  2:337737/251‑1 (MQ=255)                     aaGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGGTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTAt                                                                                                                                                                                                                        <  1:507569/251‑1 (MQ=255)                       gagGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGAAttt                                                                                                                                                                                                                      >  2:339919/1‑251 (MQ=255)                        gggCTTTTGTCGATGGGTGAATTATTCGCGCTGTGCGTGCCAACTTGTGCGCGGTCCTGCGTGACATTCTCTGCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTGTCCTGGTCTTTCCCATCTGGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGTGCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTAttt                                                                                                                                                                                                                      <  2:361361/249‑1 (MQ=255)                               tGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGCGTATTTGTATTccc                                                                                                                                                                                                              >  2:439356/1‑251 (MQ=255)                               tGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGccc                                                                                                                                                                                                              >  1:516580/1‑251 (MQ=255)                                  cGATGGGCTAATTATTCGCGCTTTGCAGGCCACCTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAAGCCATAACACCTTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTAGTCTTTTCCATCTTGCGTAACGCTCGTGCGCTGCTTGTTGGGGAAGCGCCGAAGATGGTGGGCTGCTGTGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCcgt                                                                                                                                                                                                            <  1:315332/250‑1 (MQ=255)                                  cGATGGGCGACTTATTCGCGATTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCCAATCCATAACACCGTTCGTTATCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCGGCATGAGGGTGAAGCGCCAAATATGGTGGTCGGCTGGGGAGGTCACTCAATTAACGAAAAAGAGAATTTGTATGCCcggg                                                                                                                                                                                                           >  2:337812/1‑249 (MQ=255)                                           aaTTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGaa                                                                                                                                                                                                  <  1:409541/251‑1 (MQ=255)                                               attCGCGATTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGaaccc                                                                                                                                                                                               >  2:23491/1‑247 (MQ=255)                                                        ttGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGGTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACAGCACTAACCTGGTCGTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGcc                                                                                                                                                                                      <  2:8149/250‑1 (MQ=255)                                                         tGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCt                                                                                                                                                                                     <  1:150948/250‑1 (MQ=255)                                                            aGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGt                                                                                                                                                                                 >  1:472134/1‑251 (MQ=255)                                                              gCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACCCCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGCAGGTCACTCAATTAACGAAAACGAGTCTTTGTATACCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTg                                                                                                                                                                                >  1:531624/1‑250 (MQ=255)                                                                      gTTCGCGGTCATGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAat                                                                                                                                                                        >  2:213310/1‑250 (MQ=255)                                                                                                tCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGTACTGCATATCTGCACCGGCtggtgctcgggggcg                                                                                                                                             >  2:149461/1‑238 (MQ=255)                                                                                                 cGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGa                                                                                                                                            <  1:23491/251‑1 (MQ=255)                                                                                                  gTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGGGGTCCGGGAGCg                                                                                                                                             >  2:173632/1‑249 (MQ=255)                                                                                                      aCGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTACGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTTGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGa                                                                                                                                        >  1:532009/1‑250 (MQ=255)                                                                                                           cAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGcc                                                                                                                                  >  2:86658/1‑251 (MQ=255)                                                                                                                    aaCACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGAGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAgg                                                                                                                         >  1:217887/1‑251 (MQ=255)                                                                                                                     acacCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGt                                                                                                                        <  1:279892/251‑1 (MQ=255)                                                                                                                        ccGTTCGTTTTCCCAACCTGAATCTCGACATCTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTACCGCTCGCGCGCTGCATGTGGGTGAAGCGTCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGtgct                                                                                                                     <  2:89101/251‑1 (MQ=255)                                                                                                                        ccGTTCGTTTTCCCAACCTGAATCTCGACAACGCCGTCCTCATCACTATCCTGGTCTTTGCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGtgct                                                                                                                     <  2:468425/251‑1 (MQ=255)                                                                                                                         cgttcgttTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGtgctg                                                                                                                    <  1:92024/251‑1 (MQ=255)                                                                                                                             cgttTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATCTCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGt                                                                                                                >  2:80549/1‑251 (MQ=255)                                                                                                                                tttCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGAGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGGGCGGTGGAAGCGCCGATGAAAGGTGCTGCGGTCgg                                                                                                             >  2:29201/1‑251 (MQ=255)                                                                                                                                  gcccAACCTGAACCCCGACAACCCCTTCCACATCACTAACCTGGTCGTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGTCAAATATGGCGGGCTGCTGGGGAGGTCACTCAACTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTTCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGac                                                                                                           <  1:512696/250‑1 (MQ=255)                                                                                                                                    ccAAACTGAATCTCGACAACCCCGTCCACATCACTAACCTGGACTTTTCCATCTTGCGTAACGCGCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAAAGACAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGGGAGCTGAATATCTGCACCGGCTAGGGTGCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCgggcgacccg                                                                                                         >  2:235857/1‑243 (MQ=255)                                                                                                                                           gAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCgcag                                                                                                   <  1:436675/250‑1 (MQ=255)                                                                                                                                               ctcGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCg                                                                                              >  1:466753/1‑251 (MQ=255)                                                                                                                                                 cGACAACTCCGTCCCCATCACTTACCGGGTCTTTTCCATCTGTCGTAACGCTCGCGCGCTGCATGTGGGGGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCTCCTATGAAAGGTGCTGCGGTCGTACACGCGcagcag                                                                                                <  2:510933/247‑1 (MQ=255)                                                                                                                                                 cGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATTGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGt                                                                                             <  1:227285/250‑1 (MQ=255)                                                                                                                                                  gACAACTCCGTCAACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCTCTGCATGTGGGTGAAGCGCCAAATATGGTGGTATGCTGGGGAGGTCACTCAATTAACGCAAACGAGTATTTGTATGCCCGTCGCGTCGGCAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGAGGGCGGACACGCGGAGCCGCGttt                                                                                           >  2:241172/1‑250 (MQ=255)                                                                                                                                                   aCAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTAc                                                                                          <  1:189136/251‑1 (MQ=255)                                                                                                                                                    cAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTAc                                                                                          >  1:303318/1‑250 (MQ=255)                                                                                                                                                    cAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGACTGCTGGGGAGGTCACTCAATTAACGAACACGAGTATTTGTATGCCCGTCGCGTCGGCAACCAGCTGGGCCTGCGGGAGCTGGATATCTGCACCGGCTGTGGGCCGGGAGCGATGGAAGCGCCGCTGAAAGGGGCTGCGGTCGGACACGAGCAGCCGCGTTaa                                                                                          >  2:49270/1‑249 (MQ=255)                                                                                                                                                              cacaTCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATTTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACGCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCg                                                                               <  2:488264/251‑1 (MQ=255)                                                                                                                                                              cacaTCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCg                                                                               >  1:121267/1‑251 (MQ=255)                                                                                                                                                                   cACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGtttt                                                                           <  1:213310/250‑1 (MQ=255)                                                                                                                                                                                ttttCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGATGAGGATGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCGGAATATCTGCACCAGCTGGGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGAGCTGCGGTCGGAAACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGGATGAc                                                                >  1:218537/1‑248 (MQ=255)                                                                                                                                                                                  ttCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGCTGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGc                                                           >  1:240906/1‑251 (MQ=255)                                                                                                                                                                                        cTTGCATAACGCTCACGCGCTGCATGTGGGCGAAGCGCCAAATATGGTGGTCTGCTTGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGAAGCGATGGAAGCGCCGATGAAAGGTGCAGCGGGCGGACACGCGCAGCAGCGTTACAAAGACCGTCGTTTTATTGGTATGaacaatccgcagc                                                     >  2:511339/1‑239 (MQ=255)                                                                                                                                                                                          tGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCTGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGAt                                                    <  1:164365/250‑1 (MQ=255)                                                                                                                                                                                           gCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGAtt                                                   <  1:66303/250‑1 (MQ=255)                                                                                                                                                                                           gCGTAACGCTCGCGCGCGGCATGTGGGGGAAGCTCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGGATTTGTATGCCCGTGGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGGCGTTTTATTGGTATGACAGAGCCGTCGATTa                                                  <  2:528022/251‑1 (MQ=255)                                                                                                                                                                                                  gCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGCCACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACCGAGCAGACGATTATcgcagc                                           >  2:476815/1‑248 (MQ=255)                                                                                                                                                                                                     cgcgcgCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGCGCCGACGATTATCGCCGCTg                                         >  2:266512/1‑250 (MQ=255)                                                                                                                                                                                                       cgcgcTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGATGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAAc                                      <  1:14990/251‑1 (MQ=255)                                                                                                                                                                                                        gcgcTGCATGGGGGTGAAGCGCCAAATATGGGGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGGCGCGTCGGAAACCAGCTTGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGATAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAAcc                                     <  2:18472/251‑1 (MQ=255)                                                                                                                                                                                                         cgcTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGt                                                                                                                                                      >  2:218406/1‑137 (MQ=255)                                                                                                                                                                                                         cgcTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGt                                                                                                                                                      <  1:218406/137‑1 (MQ=255)                                                                                                                                                                                                         cgcTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCg                                    >  1:233213/1‑251 (MQ=255)                                                                                                                                                                                                          gcTGCATGTGGGTGAAGCGCCAAATATTGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGc                                   >  1:528507/1‑251 (MQ=255)                                                                                                                                                                                                             gCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCt                                 <  1:86658/250‑1 (MQ=255)                                                                                                                                                                                                                       gAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGt                      <  1:339919/251‑1 (MQ=255)                                                                                                                                                                                                                                             gctgGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCAGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGc  <  1:173632/249‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  GGAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGC  >  NC_000913/2926529‑2927013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑