Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,301,992 A→T N271Y (AAT→TAT)  oppA → oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,301,9920AT98.3% 148.2 / NA 62N271Y (AAT→TAT) oppAoligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  major base T (34/25);  minor base G (0/1);  total (35/27)
Fisher's exact test for biased strand distribution p-value = 4.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

TCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATAC  >  NC_000913/1301746‑1302224
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                          
ccAACTTCACCTGTCCCAAAAGCCGCTATTGAGAAATTCGGCGAAAAATGGATCCAGCCTGGTAATAGCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATaa                                                                                                                                                                                                                                        <  2:411587/250‑1 (MQ=255)
 cAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAAc                                                                                                                                                                                                                                       <  1:513472/251‑1 (MQ=255)
  aaCTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGCCGTATAAc                                                                                                                                                                                                                                       >  2:133131/1‑250 (MQ=255)
   aCTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGCAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAAc                                                                                                                                                                                                                                       >  1:466281/1‑249 (MQ=255)
         ccGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACAGCTACCGTAGTGGTGAAATCGACCTGACGTATAACAGCAtacc                                                                                                                                                                                                                               >  2:357028/1‑248 (MQ=255)
          cGGTGCCAATAGCCGCTAACGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCg                                                                                                                                                                                                                              <  1:288921/251‑1 (MQ=255)
           ggTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGGCGTATAACAGCATGCCGa                                                                                                                                                                                                                             <  2:75481/251‑1 (MQ=255)
            gTGCCAAAAGCAGCTATCGAGAAATTCGGCGAACAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCTAAAACCTTTATAAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGAGGTGAATGCGACATGACGTATAACAGCAAGCCGAt                                                                                                                                                                                                                            >  2:362038/1‑251 (MQ=255)
              gCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCg                                                                                                                                                                                                                          <  1:148276/251‑1 (MQ=255)
                  aaaGCCGCTATCGAGAAAATCCGAGAAAAATGGGTCCAGTCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTGGTTGTGAAATGGTCATGACGTATAACAGCATGCCGATCGAAtt                                                                                                                                                                                                                      <  1:196048/251‑1 (MQ=255)
                           aTCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGa                                                                                                                                                                                                              >  1:465041/1‑250 (MQ=255)
                             cGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGc                                                                                                                                                                                                           >  1:132341/1‑251 (MQ=255)
                                        gCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTCGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAag                                                                                                                                                                                                  >  1:298502/1‑249 (MQ=255)
                                           aaaaaTGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAag                                                                                                                                                                                                  >  1:281022/1‑246 (MQ=255)
                                           aaaaaTGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAag                                                                                                                                                                                                  >  1:372734/1‑246 (MQ=255)
                                            aaaaTGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATcc                                                                                                                                                                                            >  2:343589/1‑251 (MQ=255)
                                                  gACCCAGCATGGTAATATCGTCACCAACGGTGCCAAAACCTTAAAAGATTGGGTAGTAAACGAACGAATCGTTCTTGAACGAAGCCCGACCTACTGGAACAACGCGAAAACCGATATTAACCAGGTAACCTATTTGCCTATAGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGAAGCATAACAGCATGCCGATCGCATTGTTCCAGAAGCTGCAACAAGAAATcacaga                                                                                                                                                                                        >  2:320121/1‑244 (MQ=255)
                                                       aGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTTGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGtt                                                                                                                                                                                >  1:11344/1‑251 (MQ=255)
                                                           tGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACg                                                                                                                                                                            >  2:182119/1‑251 (MQ=255)
                                                            gtgaagatCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACATCGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACg                                                                                                                                                                            <  2:412208/244‑1 (MQ=255)
                                                            ggTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCa                                                                                                                                                                              <  2:269197/248‑1 (MQ=255)
                                                            ggTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCa                                                                                                                                                                              >  1:269197/1‑248 (MQ=255)
                                                              aaaTATCGTCACCAACGGTTCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTg                                                                                                                                                                         <  2:78195/250‑1 (MQ=255)
                                                                       cACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATAcc                                                                                                                                                                >  1:129072/1‑251 (MQ=255)
                                                                       cACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATAcc                                                                                                                                                                <  2:128998/251‑1 (MQ=255)
                                                                                       atcgtaaaagtttggttactAAACGAACTAATCGTTCTTTAACCCAGCCCGACCTACTGGAACAACGCGAAACCCTTTATTAACCAGTTAACCTATGTGCCTATTGCTACTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCTTCAGGACGTATAACAGCATGTCGATCGAATTGTTCCAGAATCTGAAAAAAGATATCCTGGACGAAGTTCTCGTTTATCCATACTTGTTCTCTTACTATTa                                                                                                                                                <  2:341174/232‑1 (MQ=255)
                                                                                        ccTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTAc                                                                                                                                               >  1:374418/1‑251 (MQ=255)
                                                                                                  ttGGGTCGTACACGAACGAATCGTTCTTGACCGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTCTTTGCCTATTGCTTCTGAAGTTACCGCTGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGACGTTCAAGTTGATCCATACCTGTGCACTTACTATTACGAAATTaac                                                                                                                                      >  2:336044/1‑250 (MQ=255)
                                                                                                             acgaacgaATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCg                                                                                                                          <  2:174336/251‑1 (MQ=255)
                                                                                                               gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGcc                                                                                                                        >  1:138131/1‑251 (MQ=255)
                                                                                                                         ttcttacaCGCAGCCCGACCTACTGGAACAACTCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCAGAAGTTACCGATGTCAACCGCTACCGTAGTGGAGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGATCCTGAAGCTTAAAAAAGAGATCCAGGACGCAGTTCACGATGATCCATACCTGAGCACTTACTATTACGAAATTCACAACCAGAAAACGACATTCACCGAt                                                                                                              >  2:534248/8‑251 (MQ=255)
                                                                                                                                    aGCCCTACCTACGGGAAAAACGCGAAAACCGTTATTAACCAGGTACCCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCACCCGCTACCGTAGGGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATCCCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGAtg                                                                                                             <  2:349214/241‑1 (MQ=255)
                                                                                                                                    aGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGAtg                                                                                                             >  1:349214/1‑241 (MQ=255)
                                                                                                                                        cGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTATGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCCACGATGTGCGTTTGCGTAcc                                                                                               >  2:201166/1‑251 (MQ=255)
                                                                                                                                          aCCTACTGGAACAACTCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGc                                                                                             >  1:96882/1‑251 (MQ=255)
                                                                                                                                          aCCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTCCTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGc                                                                                             >  1:76412/1‑251 (MQ=255)
                                                                                                                                            cTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCAc                                                                                           >  1:412448/1‑251 (MQ=255)
                                                                                                                                            cTACTGGAACAACGCGAAAAACGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTAACGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATAGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATCCATGTGCACTTCCTATTACGAAATTAACAACCAGAACCCGCAATTCAACACTGAGCGTGTGCGTACCGCAc                                                                                           >  1:227413/1‑251 (MQ=255)
                                                                                                                                                              aaCCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGAcc                                                                         <  1:182119/251‑1 (MQ=255)
                                                                                                                                                              aaCCGTTACAAACTAGGTAACCTATTTGCCTATTGCGTCTGAATTTACCGATGTCAACCTCTACCGTAGTGGTGAACTCGACATGCCGTATAACATCTTGCCGATCGAATTGTTCCAGAATCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATCCCTGTGCACTTACGATTCCAAAATTAACATCCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGAcc                                                                         <  1:504686/251‑1 (MQ=255)
                                                                                                                                                                 cgtatataaccatatAACATATGTGCCTATGGGTTCTGAAGTGCCAGATGCAAACCGCTACCGTAGGGGAGAAAGTGAAATGAGTTATAACAGCATGCCGATCGAATTGTACCAGAAGCTGAAAAAAGAGACCCCGGACAAATTTCCCGTGGTACCATCCCGGTGCCATTACTATTCTGAAATTAATAACAAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCTCACTGAAACGAGGTATGGACcgcg                                                                      <  2:168890/237‑1 (MQ=255)
                                                                                                                                                                 cGTTTTTAACCAGTTACCCTATTTGCACATGTCTTCTAAATTCACCGTTGTCTACCGCTCCCGTAGGGGTCAAATCGACATGACGTATAACATCATGCCGATCTAATTGTCCCAGAAGCTCAAAAAAGAGTTCCTGGACGAAGTTCACGTTGACCCATACCTGTCCACTTACCTTTACAAAATGAACACCCAAAATCCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTAAGGACcgcg                                                                      <  2:413618/251‑1 (MQ=255)
                                                                                                                                                                    gattaACCAGGTAACCTATTGGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCATAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTTTGCGTACCGCACTGAAACTAGGTATGGACCGCGat                                                                    <  1:362038/249‑1 (MQ=255)
                                                                                                                                                                     attaACCAGTTACCCGATTTGCCTATTGCTTCTTAAGTTACCTATGTCAACCGCTACCGAAGTGGGGAAATCGACATGACGGATAGCAGCAGGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGAAGAAGTTCACGTGGATTCATTCCTGTGCACTTACTAGTACGAAATTAACAACCAGAATCCGCCATTCAACGATGGGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGatat                                                                  <  1:529722/251‑1 (MQ=255)
                                                                                                                                                                           cAGGTACCCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGGGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCAGTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGt                                                            <  1:343589/251‑1 (MQ=255)
                                                                                                                                                                            aGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGtt                                                           <  1:134945/251‑1 (MQ=255)
                                                                                                                                                                             ggTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGtt                                                           >  1:82316/1‑250 (MQ=255)
                                                                                                                                                                                aaCCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTtaat                                                        >  2:474894/1‑250 (MQ=255)
                                                                                                                                                                                 aCCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTtaata                                                       >  1:364745/1‑250 (MQ=255)
                                                                                                                                                                                    tATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGt                                                   >  1:6929/1‑251 (MQ=255)
                                                                                                                                                                                     tttggcttatggctgcTGAAGTTACCGATGTTAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAATAGAACCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATGACGAAATTAACAACCAGAACCCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGt                                                   <  2:138131/235‑1 (MQ=255)
                                                                                                                                                                                              ttGCTTCTGAAGTTACCGATGTCAACCGCTACTGTAGTGGTGAAATCGACATGACGTATAACAGCATGCAGATCGAATTGTTCAAGAAGCTGAAAAAAGAGATCCCGGACGAAGTACACCTTGATCCATATCTGTGCACTTACTATTACGACATTAACACCCAGAAACCGCCGTTCAACGATGTGCGTATGCGTCCCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTAAAAgcgc                                           >  2:380369/1‑249 (MQ=255)
                                                                                                                                                                                                          ttACCGAGGTCCACCGCGTACGTGGTGGTGAAACCGACTTGACGTATATCAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTACCCGTTGATCCATACCTGGGCACTTACTAGTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCTTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGcc                              <  2:466281/250‑1 (MQ=255)
                                                                                                                                                                                                           tACCGATGCCACCCGCTACCGTAGTGGTGAAATCTACATGACTAATAACAGCAGGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCAGGACGAAGTCCACGTTGATCCATACCTGTGCACTTATTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTTAATGCGCAGGGCAACATGccc                             <  2:412448/250‑1 (MQ=255)
                                                                                                                                                                                                                aTGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCt                         >  1:517750/1‑249 (MQ=255)
                                                                                                                                                                                                                   tCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTATGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTaggt                     >  1:526368/1‑247 (MQ=255)
                                                                                                                                                                                                                    cAACCGCTACCGTAGTGGTGAAACCGACATGCCGTATAACAGCATGCCGATCGAATTGTCCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATCCCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTaggtat                   <  1:336044/251‑6 (MQ=255)
                                                                                                                                                                                                                    acaacctgtACCGAAGGGGTAAAATCGATATAACGTATAACAGAATGCCGATAGAATGGTTCAAGAATCTAAAAAAAAAGACCCGGTACGAAGTTCCCGTTGACCCAATCCGGGCCATTAACTATATCGAAATTAACACCAAAAAACCGCCTTCCAACGATGGGCGTGTGCGTACCGCACTGAAACTAGGTATGGAACGCGATATCATTGTTAAGAAATTGATAGCGCAGGGCAACATGCCCGCCTaggt                     <  1:380369/242‑4 (MQ=255)
                                                                                                                                                                                                                     aaCCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGCGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGCAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTaggtat                   >  2:518981/1‑245 (MQ=255)
                                                                                                                                                                                                                     aaCCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTaggtat                   >  1:165517/1‑245 (MQ=255)
                                                                                                                                                                                                                                  aGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTAGGTATACACTCCCCCGt      >  2:289306/1‑250 (MQ=255)
                                                                                                                                                                                                                                     ggtgAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTAGGTATACACTCCACCGTATAc  <  1:133131/251‑1 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                          
TCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATAC  >  NC_000913/1301746‑1302224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: