Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,287,294	C→G	noncoding (35/85 nt)	tyrV ←	tRNA‑Tyr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,287,294	0	C	G	100.0%	63.3 / NA	21	noncoding (35/85 nt)	tyrV	tRNA‑Tyr
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (10/11);  total (10/11)

AAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACC‑AGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACA  >  NC_000913/1287059‑1287501                                                                                                                                                                                                                                            |                                                                                                                                                                                                                 aaaGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTGCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATGTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTg                                                                                                                                                                                                   <  1:536364/251‑1 (MQ=255)                                       gcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACC‑AGGGGTAATTCAAATTTTg                                                                                                                                                            <  1:18516/251‑1 (MQ=255)                                        cgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGCACCTTGGGCGAAGCTTCTCATCCTTCCCCGCCTGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGTAGTCGATGACGGCAGATTTAGAGTCTGCTCGCTTGGGCCGCCCGGGGACACCCCC‑AGGGGCAATTCACATTTTg                                                                                                                                                            >  1:427463/1‑250 (MQ=18)                                                     gggTCGGTGCCGGCGGCACAGCTCGCTCGTCGTCGCTCGAGGCGAACCTGGGTCGTAGCGTATGCTCCTTACACGCATTGGCAGAATAGTGAATTGCGGAGTCGTAGGTAAGTTCAGGGAATTTTGAAAGTGATGGTGGTGTGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCGCGGGAACCCCACC‑AGGGGTAATTCAAATTTTGAGGTAATGATTgag                                                                                                                                              <  2:173789/251‑1 (MQ=16)                                                         cggtgcctgcgtcgtcGCTTTCTCTCTGGCTCTCGTGTCGAACTTTGGTCGAAGTTTCTGATCCGTCTCCGCATGGGCATAATATTTAATTGCGTCTTCGGTGGGAAGTTTAGGTACTTTTGGAAGTGATGGTGGTGGGGGAAGGATTCGACCCTTCGAAGTCTATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACC‑AGGGGTAATTCAAATTTTGAGGTAATGCTTGAGAtgg                                                                                                                                          <  1:219072/236‑1 (MQ=21)                                                                 gCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGGCGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGACCCCCACCCTGGGGTA‑TTCTAATTTTGTGGtcagccttgtgatggggctgggg                                                                                                                                  >  2:258836/1‑229 (MQ=21)                                                                 gCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACC‑AGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTggg                                                                                                                                   <  1:351524/250‑1 (MQ=255)                                                                      aaCGCGCTCTCGCTGGCGCGCGAGGCGACCCTTGGGCGAAGCTTCTCATCCTTCCCCGCGTGGGCAGAATATTTGATGGCGGATTCGTTGGGAAGGTCAGGGGCGTTTGAAAGTGAGGGTGGGGGGGGAAGGATTCGAAGCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACC‑AGGGGTAAGTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAgg                                                                                                                             <  2:100644/251‑1 (MQ=25)                                                                               tcGCTGGCGCTCGAGTCGAACCTTGGTAGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCCCC‑AGGGGTAATTCCATTTTTGCGGTCTTGCTTGTGATGGGGGTGGGGGCAGGATTATtcgt                                                                                                                    >  2:226899/1‑251 (MQ=25)                                                                                                  aCCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACC‑AGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCtt                                                                                                 <  1:24022/251‑1 (MQ=255)                                                                                                                   gcagccTTCCCCGCATGGGCAGAATATTTAATTGCTGAGTCGATGGGTAGTTTAGGGATCTTGGAAAGTGTTGGTGGTGGGGGAAGGATTCTAGCCTACTAAGTCGAGGACGGCAGATGTAGAGGCTGCGATCTTTGGCCGTGTGGGAATCGTACG‑AGGGGTATTTCAAATTTGGAGGTATTGCTTGAGATGGGGGTGGGGGAAGGTTTATTCGTGGCTTCGCGCCTCACCCTTCGGGTCGTTGCCtgtg                                                                                 <  2:341876/246‑1 (MQ=255)                                                                                                                            cccGCATGGGCAGAATCTTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACC‑AGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTtct                                                                       >  1:379067/1‑251 (MQ=255)                                                                                                                            cccGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAATTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACC‑AGGGGTAATTCAAATTTTGAGGTAATGCTTGAGCTGGTGGTGGGGGCAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTtct                                                                       >  2:456938/1‑251 (MQ=255)                                                                                                                                         aaTATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACC‑AGGGGTAATTCAAATTTTGTGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTTCCTGAGGCAACGTTCTCTCGCTTTCGCtc                                                          >  1:318993/1‑251 (MQ=255)                                                                                                                                           tatTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGCAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGCAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACC‑ACGGGGAATTCCAATTTTCTGGTCATGCTTCAGATGGGGGTGGGGGACGCATTATTTCTCGTTTCGCTCGTTACCCTTCGGGCCGTGGCCGGTGGGAATGTTCTCTCGCTTTGGCtccg                                                        >  2:471125/1‑249 (MQ=18)                                                                                                                                                           cGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACC‑AGGGGTAATTAAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGTCCGTTGCATGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCg                                         >  1:354228/1‑250 (MQ=255)                                                                                                                                                                ggAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACC‑AGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGtt                                   <  1:188583/251‑1 (MQ=255)                                                                                                                                                                          gtatgtgtGAAAGTGATGGTGGGGGGGCAAGGATTCGAACCTTCGATGTCGATGACGGCAGATTTAGAGTGTGCTCCCTTTTGCCGCTCGTGAACCCCGCC‑AGGGGTAATTCAAATTTGGAGGTAATGCGTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCGTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCTAATCGAACCTTAGTCGAAGGTTCTCACCCTTc                         <  2:379067/244‑1 (MQ=255)                                                                                                                                                                                ttGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACC‑AGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGAt                   >  1:7249/1‑251 (MQ=255)                                                                                                                                                                                 tGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACC‑AGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATg                  >  1:154221/1‑251 (MQ=255)                                                                                                                                                                                                 gggggAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGATCCCTTTGGCCGCTCGGGAACCCCACC‑AGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTcaca  <  1:456938/251‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                 AAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACC‑AGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACA  >  NC_000913/1287059‑1287501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑