Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,001,251	A→C	Q679P (CAA→CCA)	elfC →	putative fimbrial usher protein ElfC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,001,251	0	A	C	89.1%	120.2 / ‑1.6	46	Q679P (CAA→CCA)	elfC	putative fimbrial usher protein ElfC
Reads supporting (aligned to +/- strand):  ref base A (3/2);  new base C (23/18);  total (26/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.02e-01

AGCTATACCAGGAAAAAAAATTACGCCTGGATGACCTCTAACACCAGTATCGATAACGAAGGGCACACTACACAAAACCTGGGTTTAACGGAGACATTACTCGATGACGGTAACCTGAGCTACAGCGTGCAACAGGGATATAACAGCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACAACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCA‑CAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCACCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGC  >  NC_000913/1001004‑1001497                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                        cgCTATACCAGGAAAAAAAATTACGCCTGGATGACCTCTAACACCAGTATCGATAACGAAGGGCACACTACACAAAACCTGGGTTTAACGGAGACATTACTCGATGACGGTAACCTGAGCTACAGCGTGCAACAGGGATATAACAGCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAAATACAGAGATAACGGCAGTAAACAACCACt                                                                                                                                                                                                                                                      >  2:327582/2‑251 (MQ=255) aGCTATACCAGGAAAAAAAATTACGCCTGGATGACCTCTAACACCAGTATCGATAACGAAGGGCACACTACACAAAACCTGGGTTTAACGGAGACATTACTCGATGACGGTAACCTGAGCTACAGCGTGCAACAGGGATATAACAGCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACAACt                                                                                                                                                                                                                                                      <  1:164450/251‑1 (MQ=255)     ataCCAGGAAAAAAAATTACGCCTGGATGACATCGAACACCAGTATCGATAACGAAGGGCACACTACACAAAACCGGGGTTTAACGGAGACAGTACGCGATGACGGTAACCTGAGCTACAGCGTGCAACAGGGATATAACAGCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGGTTGCGGATGCCCGAGTGGGCTACAACGACAGCGATAACGGCAGACAACAACCACTGAAc                                                                                                                                                                                                                                                  <  2:289459/251‑1 (MQ=255)     ataCCAGGAAAAAAAATTACGCCGGGATGACCTCTAACACCAGTATCGATAACGAAGGGCACACTACACAAAACCTGGGTTTAACGGAGACATTACTCGATGACGGTAACCTGAGCTACAGCGTGCAACAGGGATATAACAGCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGa                                                                                                                                                                                                                                                    <  1:252817/249‑1 (MQ=255)        ccAGGAAAAAAAATTACGCCTGGATGACCTCTAACACCAGTATCGATAACGAAGGGCACACTACACAAAACCTGGGTTTAACGGAGACATTACTCGATGACGGTAACCTGAGCTACAGCGTGCAACAGGGATATAACAGCGAGGGGAAACCGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTAc                                                                                                                                                                                                                                               <  1:492609/251‑1 (MQ=255)            gAAAAAAAATTACGCCTGGATGACCTCTAACACCAGTATCGATAACGAAGGGCACACTACACAAAACCTGGGTTTAACGGAGACATTACTCGATGACGGTAACCTGAGCTACAGCGTGCAACAGGGATATAACAGCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACACCCACTGAACTACGctc                                                                                                                                                                                                                                           >  1:462645/1‑251 (MQ=255)                   aaTTACGCCTGGATGACCTCTAACACCAGTATCGATAACGAAGGGCACACTACACAAAACCTGGGTTTAACGGAGACATTACTCGATGACGGTAACCTGAGCTACAGCGTGCAACAGGGATATAACAGCGAGGGGAAAACGGCTACTGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCCGTCAACAACCACTGAACTACGCTCTTTCAgg                                                                                                                                                                                                                                    >  2:418653/1‑251 (MQ=255)                       aCGCCTGGATGACCTCTAACACCAGTCTCGATAACGAAGGGCACACTACACAAAACCTGGGTTTAACGGAGACATTACTCGATGACGGTAACCTGAGCTACAGCGTGCAACAGGGATATAACAGCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGt                                                                                                                                                                                                                                >  1:355206/1‑251 (MQ=255)                             ggATGACCTCTAACACCAGTATCGATAACGAAGGGCACACTACACAAAACCTGGGTTTAACGGAGACATTACTCGATGACGGTAACCTGAGCTACAGCGTGCAACAGGGATATAACAGCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGTTTAGtt                                                                                                                                                                                                                          <  1:240379/251‑1 (MQ=255)                                                  tCGATAACGAAGGGCACACTACACAAAACCTGGGTTTAACGGAGACATTACTCGATGACGGTAACCTGAGCTACAGCGTGCAACAGGGATATAACAGCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCA‑CAGGACATTAcc                                                                                                                                                                                                    >  1:202652/1‑251 (MQ=255)                                                         cGAAGGGCACACTACACAAAACCTGGGTTTAACGGAGACATTACTCGATGACGGTAACCTGAGCTACAGCGTGCAACAGGGATATAACAGCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCA‑CAGGACATTACCCTGGGGc                                                                                                                                                                                             <  2:202652/251‑1 (MQ=255)                                                          gAAGGGCACACTACACAAAACCTGGGTTTAACGGAGACATTACTCGATGACGGTAACCTGAGCTACAGCGTGCAACAGGGATATAACAGCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCA‑CAGGGCATTACCCTTGGGc                                                                                                                                                                                             >  2:225830/1‑250 (MQ=255)                                                             gggCACACTACACAAAACCTGGGTTTAACGGAGACATTACTCGATGACGGTAACCTGAGCTACAGCGTGCAACAGGGATATAACAGCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCA‑CAGGGCATTACCCTGGGGCAATc                                                                                                                                                                                         >  1:356781/1‑251 (MQ=255)                                                                cacaCTACACAAAACCTTGGTTTAACGGAGACATTACTCGATGACGGTAACCTGAGCTACAGCGTGCAACAGGGATATAACAGCGAGGGGAAAACGGCTAATGGTATCGCCAGCATGGATTACAAAGGTGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCAGTCG‑CAGGGCATTACCCTGGGGCAATCGCt                                                                                                                                                                                      <  2:523165/251‑1 (MQ=255)                                                                cacaCTACACAAAACCTGGGTTTAACGGAGACATTACTCGATGACGGTAACCTAAGCTACAGCGTGCAACAGGGATATAACAGCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCCGGCAGTTTAGTTGCCCATTCA‑CAGGGCATACCCCTGGGGCAATCGCt                                                                                                                                                                                      >  2:357779/1‑251 (MQ=255)                                                                                  ggTTTAACGGAGACATTACTCGATGACGGTAACCTGAGCTACAGCGTGCAACAGGGATATAACAGCTAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCA‑CAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCt                                                                                                                                                                    >  1:268371/1‑251 (MQ=255)                                                                                   gTTTAACGGAGACATTACTCGATGACGGTAACCTGAGCTACAGCGTGCAACAGGGATATAACAGCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCA‑CAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTg                                                                                                                                                                   <  1:256816/251‑1 (MQ=255)                                                                                      tAACGGAGACATTACTCGCTGACGGTAACCTGAGCTACAGCGTGCAACAGGGATATAACAGCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTAAGGCCGTTTAGTTGCCCATTCA‑CAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGAtt                                                                                                                                                                >  2:521956/1‑251 (MQ=255)                                                                                             gaCATTACTCGATGACGGTAACCTGAGCTACAGCGTGCAACAGGGATATAACAGCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCA‑CAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCAc                                                                                                                                                         <  1:98133/251‑1 (MQ=255)                                                                                             gaCATTACGCGATGACGGTAACCTGAGCTACAGCGTGCAACAGGGATATAACAGCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTGTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCA‑CAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCAc                                                                                                                                                         <  2:506568/251‑1 (MQ=255)                                                                                              aCATTACTCGATGACGGTAACCTGAGCTACAGCGTGCAACAGGGATATAACAGCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACAACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCA‑CAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCAcc                                                                                                                                                        >  1:497793/1‑251 (MQ=255)                                                                                                 ttACTCGTTGACGGAAACCTGAGCTACAGCGTGCAACAGGGATATAACAGCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATACCGGCAGTACACAACCACTGAACACCGCTCTATCAGGCAGTTTTGTTGACCATTCA‑CGGGGCATTACCCTGGGGCAATCGCTGGGGGAAAATACAGTTAAGATTGCAGCACCAgg                                                                                                                                                     >  2:324725/1‑251 (MQ=255)                                                                                                        aggACGGTAACCTGAGCTACAGCGTGCAACAGGGATATAACAGCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCA‑CAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCagag                                                                                                                                              <  2:300207/249‑1 (MQ=255)                                                                                                             ggTAACCTGGGCTACAGCGTGCAACAGGGCTATAACAGCGAGGGGCAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTTCGGATGCCCGAGTGGGCTACAACTACAGCGTTAACGGCCGTCAACAACCACTTAACTAATCTCTTTCCGGTATTTTAGATGCCCCTTCAACAGG‑CATTCCCCTTGGTGACTCGCTGTGGGAAACTAACGTTCTGATTGCAGCACCAggaccactgacta                                                                                                                                          >  2:221670/1‑239 (MQ=255)                                                                                                                aaCCTGAGCTACAGCGTGCAACAGGGATATAACAGCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCA‑CAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCg                                                                                                                                      <  1:98162/251‑1 (MQ=255)                                                                                                                         tACAGCGTGCAACAGGGATATAACAGCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCA‑CAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCCGAGAATACTCGTGTGGCGa                                                                                                                              >  1:391524/1‑250 (MQ=255)                                                                                                                           cggCGTGCAACAGGGATATAACCGCGGGGGGAAACCGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTAGCGGATGCCCGAGGGGGCTACAACTACAGCGATAACGGCAGTAAACAACACCTGAACTACGCTCTTACAGGCAGTTTAGGTGCCCATTCA‑AAGGGCATTAACCTGGGGCAATCGCTGGGGGAAACTATCGTTATGCATGCAGCACAAGGCGACGAGAATACACGTGAGGCGAAc                                                                                                                            >  2:20551/3‑250 (MQ=255)                                                                                                                           cAGCGTGCAACAGGGATATAACAGCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCA‑CAGGTCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAAc                                                                                                                            >  2:169059/1‑250 (MQ=255)                                                                                                                                                            aaCGACTAATGGTAGGGTCAGTATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACGACAGCGATACCGGCAGTCAACAACCACTGAACGACGCGCTTTCAGGCAGGTTAGTTGCCCATTCC‑CAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCGCCAGGCGCAGAGAATACTCGTGTGGCGAACAGCACCGGGCTGAAATCTGCCTGTCTCGGAtata                                                                                          <  2:122862/251‑1 (MQ=255)                                                                                                                                                                  tAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCA‑CAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCACCGGGCTGAAAACTGACTGGCGCGGATATACCgttg                                                                                    >  1:484295/1‑251 (MQ=255)                                                                                                                                                                  tAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACAACTGAACTACGCCCTTTCAGGCAGTTTAGTTGCCCATTCA‑CAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTgcag                                                                                                                                                            >  2:211329/1‑179 (MQ=255)                                                                                                                                                                  tAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACAACTGAACTACGCCCTTTCAGGCAGTTTAGTTGCCCATTCA‑CAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTgcag                                                                                                                                                            <  1:211329/179‑1 (MQ=255)                                                                                                                                                                              cAGCATGGATTACAAAGGGGCGTTTGCGGATGACCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCA‑AAGGGCATTACCCTGAGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTAGTGTGGCGAACAGCACCGGGATGAAAACTGATAGGCGCGGATATACCGTTGTTCCTTATGcc                                                                         >  1:283125/1‑250 (MQ=255)                                                                                                                                                                                       ttACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCA‑CAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCACCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATc                                                               >  1:47958/1‑251 (MQ=255)                                                                                                                                                                                        tACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGAGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCA‑CAGGGCATTACCCTGGGGCAACCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGCGAATACTCGTGTGGCGAACAGCACCGGGCTGAAAACTGACTGGCGCGGGTATACCGTTGTTCCTTATGCCACTTCTTATCg                                                              >  2:411395/1‑251 (MQ=255)                                                                                                                                                                                          aaaaGGGGCGTTGGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCA‑CAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCACCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCggg                                                            <  2:268371/250‑1 (MQ=255)                                                                                                                                                                                           aaaGGGGCGTTTGCGGATGCCCGAGTGGTCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCA‑CAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCACCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCggg                                                            >  1:276554/1‑250 (MQ=255)                                                                                                                                                                                                    tttGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCA‑CAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCACCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAaatcgaat                                                  >  1:222586/1‑251 (MQ=255)                                                                                                                                                                                                        cGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCAATTCA‑CAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGGATACTCGTGTGGCGAACAGCACCGGGCTGAAAACTGACTGGCGCGGATATCCCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGcc                                              >  2:331230/1‑250 (MQ=255)                                                                                                                                                                                                         ggATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCA‑CAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCACCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCAc                                             <  1:510001/251‑1 (MQ=255)                                                                                                                                                                                                             gCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCA‑CAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCACCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTg                                          <  1:418653/250‑1 (MQ=255)                                                                                                                                                                                                               ccGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCA‑CAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCACCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATg                                       >  1:77115/1‑251 (MQ=255)                                                                                                                                                                                                                    tGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCA‑CCAGGCATTACCCTGGGGCAATAGCTAGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGACCAGCACCGAGCTGAAAACTGACTGGCGCAGATATACCGGTGTTCCTTATGCCACTTCTTATCGGGACAATCGATTCCCCCTTGAtcagacc                                  >  2:468896/1‑245 (MQ=255)                                                                                                                                                                                                                      ggCTACAACTACAGCTGTAAAGGCAGTCAACAACCCCTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCA‑CAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCACCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAAGCGCACTTGATgcggcgcc                                <  2:77299/251‑3 (MQ=255)                                                                                                                                                                                                                                           gtaaGTCAACAACCACTGAACTACGCGCTTTCAGTCAGTTTGGTTGTCCATTTC‑CTGGTCATTACCCTGGTGCAATCGCTGGGGGAACCTAACGTGCTGATCGCAGCACCAGGCGCAGAGAATACGCGTGTGGCGAACAGCACCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCGCTTCTAATCGTGAAAATCGAATCGCACTTAATGCAGCGTCGTTAAACCGTAACGTGGATc            <  2:355206/247‑1 (MQ=255)                                                                                                                                                                                                                                            gCAGTCAACAACCACTGAACTACGCTCTTGCAGGCAGTTTAGTTGCCCATTCA‑CAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCACCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCtt          <  1:221670/251‑1 (MQ=255)                                                                                                                                                                                                                                                   acaaccacTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCA‑CAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCACCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATg   <  1:313876/251‑1 (MQ=255)                                                                                                                                                                                                                                                    caaccacTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCA‑CAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCACCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGc  >  1:288583/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                        AGCTATACCAGGAAAAAAAATTACGCCTGGATGACCTCTAACACCAGTATCGATAACGAAGGGCACACTACACAAAACCTGGGTTTAACGGAGACATTACTCGATGACGGTAACCTGAGCTACAGCGTGCAACAGGGATATAACAGCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACAACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCA‑CAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCACCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGC  >  NC_000913/1001004‑1001497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑