| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,848,333 | 0 | T | A | 24.0% | 103.5 / 10.5 | 50 | M98L (ATG→TTG) | uhpC | inner membrane protein sensing glucose‑6‑phosphate |
| Reads supporting (aligned to +/- strand): ref base T (25/13); new base A (0/12); total (25/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.57e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AATGCAGCGCAGCCGCTGCCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAA‑CCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAAC‑GCCCATAGC‑GACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCT‑ATCCCCATAAAATAACGGG‑CATTTGAGCGATCGCTGACAATGCCGGAGACAAACTT‑CGAC‑ACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCGTG‑AAGTAAAACAGCGCGTAACCCAGCCAGATGGTCAGCAGAATATGCCGACGCCAGTAGCGATAGCGGGCATCAATTTCATATTTATCAGTC > NC_000913/3848084‑3848583 | aaTGCAGCGCAGCCGTTGCCACCACAATGGAATTGAGTGCGCCGCCGTCGTTATGCCCCGTTTTCCATAATGCCCACCAA‑CCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAAC‑GCCCATAGC‑GACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCT‑ATCCCCAt < 1:125791/250‑1 (MQ=255)aaTGCAGCGCAGCCGCTGCCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAA‑CCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAAC‑GCCCATAGC‑GACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCT‑ATCCCCAt < 1:457286/250‑1 (MQ=255) cagcgcagcCGCTGCCATCACAATGGGAATGAGTGCGCCGCCGACGTTAGGCGCCGTGTTCCATAATGCCCACCAA‑CCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAAC‑GCCCATAGC‑GACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCT‑ATCCCCATAAAAt < 1:185578/251‑1 (MQ=255) ccGCTGCCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAA‑CCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAAC‑GCCCATAGC‑GACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCT‑ATCCCCATAAAATAACGGG‑c > 1:391038/1‑250 (MQ=255) cATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAA‑CCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAAC‑GCCCATAGC‑GACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCT‑ATCCCCATACAATAACGGG‑CATTTGAGc > 1:306933/1‑251 (MQ=255) caATGGGAAGGCGGGCGCCGCCGACGTTATGCGCCGTGTGCCATATTGCCCCCCAA‑CCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAAC‑GCCCATAGC‑GACGTCGAGAATCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCT‑ATCCCCATAAAATAACGGG‑CATTTGAGCGATCg < 2:544860/251‑1 (MQ=255) gtgccccGTGTTCAATAA‑GCCCCCCAAACCGCCGCGCGCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACCCACCGGGGAACCCCAGCCCTGGAAAAATGCGGTCAGCACCCAGAGCACGGCAAAC‑GCCCATAGC‑GACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCT‑ATCCCCATAAAATAACGGT‑CATTTGAGCGAGCGCTGACAATGCCGGAGACAAACTT‑CGac < 2:543087/245‑1 (MQ=255) cGTGTTCCATAATGCCCACCAA‑CCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAAC‑GCCCATAGC‑GACGTCGAGAAGCCAAACAGAATGTTGCTAATGCCCGTGGCGATAAGCCCT‑ATCCCCATAAAATAACGAG‑CATTTGAGCGTTCGCTGACAATGCCGGAGACAAACTT‑CGAC‑ACGCCAt > 2:191428/1‑251 (MQ=255) accaaaaTGCCCCCCAA‑CCGCCGCGCTCGGTACGTGAATACCATGCCGTTAACAGACGCGCACCCACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAAC‑GCCCATAGC‑GACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCT‑ATCCCCATAAAATAACGGG‑CATTTGAGCGATCGCTGACAATGCCGGAGACAAACTT‑CGAC‑ACGCCATAGGt < 1:56513/245‑1 (MQ=255) atattGCCCACCAA‑CCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAAC‑GCCCATAGC‑GACGTAGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCT‑ATCCCCATAAAATAACGGG‑CATTTGAGCGATCGCTGACAATGCCGGAGACAAACTT‑CGAC‑ACGCCATAGGTAATg < 1:439204/247‑1 (MQ=255) aTAATGCCCCCCAA‑CCGCCGAGCTAGGGACGTGAATACCAGGCAGTTAAACGACGCGCACACAACGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCCAAC‑GCCCATAGC‑GACGTCGAGAAGCCAAACCGCATGTTGATACTGCCCGTGGCGATAAGACCT‑ATCCCCATAAAATAACGGG‑CATTTGGACGATCGCTGCCACTGCCGGCGACAGCCTT‑CGAC‑ACGCCATAGGTaa > 2:271664/1‑249 (MQ=255) accaA‑CCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAAC‑GCCCATAGC‑GACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCT‑ATCCCCATAAAATAACGCG‑CATTTGAGCGATCGCTGACAATGCCGGAGACAACCTT‑CGAC‑ACGCCATAGGTCATGTAAAACAgg > 2:131175/1‑251 (MQ=255) cgccgcGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAAC‑GCCCATAGC‑GACGTCGAGAAGCCAAACAGCATGTTGATAATGCCCGTGGCGATAAGCCCT‑ATCCCCATAAAATAACGGG‑CATTTGAGCGATCGCTGACAATGCCGGAGACAAACTT‑CGAC‑ACGCCATAGGTAATGTAACACAGGGTCGCt > 2:16372/1‑251 (MQ=255) ccccgcGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGGGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAAC‑GCCCATAGC‑GACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCT‑ATCCCCATAAAATAACGGG‑CATTTGAGCGATCGCGGACAATGCCGGAGACAAACTT‑CGAC‑ACGCCATAGGTAATGTAAAACAGGGTCGCt < 2:126127/249‑1 (MQ=255) cgctcctggttcGTGAATACCAGGCCGTAAACATACGCGCACACACCGTGGAACCCCAGCCCTGGAAAAAGGCGTCCAGCACCCAGAGCACGGCAAAC‑GCCCATAGC‑GACGTCGAGAAGCCAAAACGAATGTTGATAATGCCCGGGGCGATAAGCCCT‑ATCCCCATAAAATACCGGG‑CAGGTGAGCGATCGCTGACAATGCCGGAGACAAACTT‑CGAC‑ACGCCATAGGTAATGTAAAACAGGGTCGCTAAc < 2:431858/240‑1 (MQ=255) cGGTACGTGAATACCAGGCCGTTAACAGACGCGCACCCACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAAC‑GCCCATATC‑GACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCTTGGCGATAAGCCCT‑ATCCCCATAAAATAACGGG‑CATTTGAGCGATCGCTGACAATGCCGGATACAAACTG‑CGAC‑ACGCCATAGGTAATGTAAAACAGGGGCGCTAATAGGcc < 1:435565/250‑1 (MQ=255) aCGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGAAACAGGCGTTCAGCACCCAGAGCACGGCAAAC‑GCCCATAGC‑GACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCT‑ATCCCCATAAAATAACGGG‑CATTTGAGCGATCGCTGACAATGCCGGAGACAAACTT‑CGAC‑ACGCCATAGGGAATGTAAAACAGGGGCGCTAACAGGCCGatat > 2:428524/1‑251 (MQ=255) cAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGCAAAAGGCGTTCAGCACCCAGAGCACGGCAAAC‑GCCCATAGC‑GACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCT‑ATCCCCATAAAATAACGGG‑CATTTGAGCGATCGCTGACAATGCCGGAGACAAACTT‑CGAC‑ACGCCATAGGACATGTAAAACAGGGTCGCTACCAGGCCGATATCGCTACGGCt > 2:274509/1‑251 (MQ=255) gCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAAC‑GCCCATAGC‑GACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCT‑ATCCCCATAAAATAACGGG‑CATTTGAGCGATCGCTGACAATGCCGGAGACAAACTT‑CGAC‑ACGCCATAGTTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAg < 1:532073/251‑1 (MQ=255) ttAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAAC‑GCCCATAGC‑GACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCT‑ATCCCCATAAAATAACGGG‑CATTTGAGCGATCGCTGACAATGCCGGAGACAAACTT‑CGAC‑ACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACg > 1:349185/1‑251 (MQ=255) ccgcgCACACCCCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCCCCCAGAGCACGGCAAAC‑GCCCATAGC‑GACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGGGGCGATAAGCCCT‑ACCCCCATAAAATAACGGG‑CATTTGAGCGATCGCTGACAATGCCGGAGACAAACTC‑CGAC‑ACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAg < 1:539866/250‑1 (MQ=255) acacCGGGGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAAC‑GCCCATAGC‑GACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCT‑ATCCCCAAAAAATACCGGG‑CATTTGAGCGATCGCTGACAATGCCGGAGACAAACTT‑CGCC‑ACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGAtt < 1:249401/251‑1 (MQ=255) aaCCCAAGCCCGGAAAAAAGGCGTCCAGCACCAAGAGACCTGCAAAC‑‑CCCATTGCCGACGACGAGAACCAAAAAAAAATGTTAAAAAGCCCCGGGGCGA‑AAGCCCTTATCCCCAAAAAATAACGGG‑CATTGGAGCGATCGCTGACAATGCCGGAGAAAAACTT‑CGAC‑ACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTAcg < 2:130602/251‑1 (MQ=255) cccaggaaaaaaGGCCTTCAGCACCCAGAGCACGGCAAAC‑GCCCATAGG‑GACGCCGAGAAGCAAAAAAGAAGTTTGAAAATGCCCGTGGCGAAAACCCCT‑ATCCCCAAAAAATAAGGGC‑CATTGAAGCGAGCGCTAACAAGGCCGGAGACAAGCGT‑CGAC‑ACGCCATTGGTATTTTAAGACAGGGTCGCTAACAGGCCGATATCGCTACGTCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGt < 1:42437/244‑1 (MQ=255) aaaGGCGTTCAGCACCCAGAGCACGGCAAAC‑GCCCATAGC‑GACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCT‑ATCCCCATAAAATAACGGG‑CATTTGAGCGATCGCTGACAATGCCGGAGACAAACTT‑CGAC‑ACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACttt > 1:309945/1‑251 (MQ=255) aaGGCGTTCCGCACCCAGA‑CTCGGTAAACCGCCCAGAGC‑GAGGCGGAATAGCCAAACAGAAGTTTGAAAATGCCCGGGGCGAAAAGCCCT‑TCCCCCAAAAAAAAACGGG‑CTTGTGAGCGATCGCGGACAATGCCGGAGAAAAACTT‑CGAC‑ACGCCATAGGTAATGTAAAACAGGGTCGCAAACAGGCCGATAGCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACtttt < 2:146906/251‑1 (MQ=255) ggtCAGCACCCAGAGCACGGCAAAC‑GCCCATAGC‑GACGTCGAGAAGCCAAACAGACTGTTGATAATGCCCGTGGCGATAAGCCCT‑ATCCCCATAAAATAACGGG‑CATTTGAGCGATCGCTGACAATGCCGGAGACAAACTT‑CGAC‑ACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAAATTTTCcgcg > 2:3940/3‑251 (MQ=255) tCAGCACCCAGACCGCGGCAACC‑GCCCATGGC‑ACCGTCGAGAAGCAAAACGGAATGTTGATATTCCCGGTGGCGATAACCCCT‑ACCCACAAAAAAGAACGGG‑CATTGAAGCGAACGCTGACAATGCCGGAGACAAACT‑‑GGACCACCCCATAGGTAATAAAAAACAGGGTCGCTAACAGGCCGATAGCGCTGCGGCTGAGCACGCCGTTAGCAAGGATTTCTGGCACGGCGGCGTTAAAACTTTTCCGCGt < 1:200861/250‑1 (MQ=255) aGCACCCAGAGCACGGCAAAC‑GCCCATAGC‑GACGTCGAGAAGCCAAACAGAATGGTGATAATGCCCGTGGCGATAAGCCCT‑ATCCCCATAAAATAACGGG‑CATTTGAGCGATCGCTGACAATGCCGGAGACAAACTT‑CGAC‑ACGCCATAGGTAATGTAAAACAGGGTCGCAAAAAGGCCGATCTCGCAACGGCTGAGCACGACGTTAGCAAGGATTTCGGGTACGGCGGAGTTAAAAATTTTCCGCGTG‑a > 2:287265/1‑250 (MQ=255) agagCACGGCAAAC‑GCCCATAGC‑GACGTCGAGAAGCCAAACAGAATGTTGATAATGCCAGTGGCGATAAGCCAT‑ATCCCCATAAAATAACGGG‑CATTTGAGCGATCGCTGACAATGCCGGAGAAAAACTT‑CGAC‑ACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGCACACGCTGTTAGCAAGGATTTCTGGTACGGCGGCGTTTAAAATTTTCCGCGCG‑CAGTAAAAc > 2:488184/1‑251 (MQ=255) ggCAAAC‑GCCCATAGC‑GACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCT‑ATCCCCATAAAATAACGGG‑CATTTGAGCGATCGCTGACAATGCCGGAGACAAACTT‑CGAC‑ACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGGTAGCAAGGATTTCTGGTACGGCGGCGTTAGACGTTTTCCCCGGT‑AAGTAAAACAGcgcgg > 2:204868/1‑250 (MQ=255) cgggaaaccgcccagagggccgtggagaAGCAAAACAGAATGGGGATAATGCCCGGGGGGATACGCCCT‑ATCCACAAAAAAAAACGGG‑CATTTGAGCGAGCGCGGACAAGGACGAAAACAACCTC‑CGAC‑ACGCCATAGGTAATGTAAAAGAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCGTG‑AAGTAAAACAgcgc < 2:546040/227‑1 (MQ=255) aaaC‑GCCCATAGC‑GACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCT‑ATCCCCATAAAATAACGGG‑CATTTGAGCGATCGCTGACAATGCCGGAGACAAACTT‑CGAC‑ACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCGTG‑AAGTAAAACAGCTCGTAAc > 1:251849/1‑251 (MQ=255) cccaaaaGC‑GACGTCGAGAAACCAAACATAATTGTGATAACGCCCGTGGCGTTAACCCTT‑ATCCCCAAAAAATAACGGG‑CTTTTGAGCGATCGCTGACAAGGCCGGAGACAACCTT‑CGAC‑ACGCCATAGGTAATTTAAAACAGGGTCGCTAACAGGCCGATATCGCTCCGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCGTG‑AAGTAAAACAGCGCGTAACccag < 1:458654/245‑1 (MQ=255) gC‑GACGTCGAGAGGCCAACCAGAATGTTGATAAGGCCCGGGGCGATAAGCCCT‑ACCCCCATAAAATAACGGG‑CATTTGAGCGACCGCTGACAATGCCGGAGAAAAACTT‑CGAC‑ACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCGTG‑AAGTAAAACAGCGCGTAACCCAGCCAGATg < 1:50480/251‑1 (MQ=255) c‑GACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCT‑ATCCCCATAAAATAACGGG‑AATTTGCGCGATCGCTGACAATGCCGGAGACAAACTT‑CGAC‑ACGCCATAGGTAATGAAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCGGG‑AAGTAAAACAGCGCTGAACCCAGCCAAATgg > 2:417821/1‑251 (MQ=255) cGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCT‑ATCCCCATAAAATAACGGG‑CATTTGAGCGATCGCTGACAATGCCGGAGACAAACTT‑CGAC‑ACGCCATCGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCGAG‑AAGGAAAACAACGCGTAACCCAGCCAGATGGTc > 2:49506/1‑250 (MQ=255) ccAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCT‑ATCCCCATAAAATAACGGG‑CATTTGAGCGATCGCTGACAATGCCGGAGACAAACTT‑CGAC‑ACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATCTCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGGGGG‑TAGTAAAACAGCGCGGAACACCGCCAGAGGGGCAGCAGAATATg > 2:135616/1‑251 (MQ=255) aaCAGAATGTTGTTCATCCCCGTGGCGATAAGCCCT‑ATCCCCATAAAATAACGGG‑CATTTGAGCGCTCGCTGACAATGCCGGAGACAAACTT‑CGAC‑ACGCCATCGGTAATATAAAACAGGGTCGCTAACAGGCCGATATCGCTCCGGATGTGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGGTAAAACTTTTCCGGGTGGAAG‑CAAACAGCGCGGAACCCAGACAGATGGTCGGCAGAAAATGCCg > 2:495083/1‑251 (MQ=255) ggtggATAATGCCCGTGGCGAAAAGCCTT‑ACCCCCATAAAATACCGGC‑AATTTGAGCGATCCCTGAAAATGCCGGAGACAAACTC‑CGAC‑ACGCCATAGGTAATTTAAACCAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCGTG‑AAGTAAAACAGCGCGTAACCCAGCCAGATGGTCAGCAGAATATGCCGACGCCAg < 1:215104/247‑1 (MQ=255) aaTGCCCGTGGCGATAAGCCCT‑ATCCCCATAAAATAACGGG‑CATTTGAGCGATCGCTGACAATGCCGGAGACAAACTT‑CGAC‑ACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCGTG‑AAGTAAAACAGCGCGTAACCCAGCCAGATGGTCCGCAGAATATGCCGACGCCAGTAACGAt > 1:351491/1‑251 (MQ=255) ccctgggCGATAAGCCAT‑CCCCCAAAAAAATAACGGG‑AATTTGAGCGATCGCTGAAAAGGCCGGAGACAAACTT‑CGAC‑ACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCGTG‑AAGTAAAACAGCGCGTAACCCAGCCAGATGGTCAGCAGAATATGCCGACGCCAGTAGCGATAGCg < 1:191428/246‑1 (MQ=255) cccGTGGCGATAAGCCCT‑ATCCCCATAAAATAACGGG‑CATTTGAGCGATCGCTGACAATGCCGGAGACAAACTT‑CGAC‑ACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCGTG‑AAGTAAAACAGCGCGTAACCCAGCCAGATGGTCAGCAGAATATGCCGACGCCAGTAGCGATaccg > 1:194745/1‑248 (MQ=255) ccGTGGCGATAAGCCCT‑ATCCCCATAAAATAACGGG‑CATTTAAGCGATCGCTGACAATGCCGGAGACAAACTT‑CGAC‑ACGCCATAGGAAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCCTG‑AAGTAAAACAGCGCATACACCACCCAGATGGTCAGCAGAATATGCCGACGACAGTAGCGAtcacgg > 2:505178/1‑246 (MQ=255) ccGTGGCGATAAGCCAT‑ATCCCCATAAAATAACGGG‑CATTTGAGCGATCGCTGACAATGCCGGAGACAAACTT‑CGAC‑ACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCGTG‑AAGTAAAACAGCGCGTAACCCAGCCAGATGGTCAGCAGAATATGCCGCCGCCAGTAGCGATAGCgg > 1:119090/1‑251 (MQ=255) ggctaaaaccccT‑ACCCCCAAAAAAAAACGGG‑CTTTTGAGCGATCCGTGAAAATGCCGGAGAAAAACTT‑CCAC‑ACGCCATAGGTAATGAAAAACAGGGACGCAAACAGGCCGATATCGCTACGTCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCGTG‑AAGTAAAACAGCGCGTAACCCAGCCAGATGGTCAGCAGAATATGCCGACGCCAGTAGCGATAGCGGGCAt < 2:391038/242‑1 (MQ=255) cGATAAGCCCT‑ATCCCCATAAAATAACGGG‑CATTTGAGCGATCGCTGACAATGCCGGAGACAAACTT‑CGAC‑ACGCCATAGGTAATGTAAAACAGGGACGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGATAAAACTTTTCCACGGG‑AAATAAAACAAGGCGTAACCCCGCCAGAAGGTCAGCCGAATATGCCGACgaccgatggcaaagcggggatc > 2:358055/1‑229 (MQ=255) aaGCCCT‑ATCCCCATAATA‑AACGGGGCATTTGAGCGATCGCTGACAAGGCCGAAGA‑AAACTTTCGAC‑ACGCCATAGGTAATGTAAAACGGGGTCGCTAACAGGCCGAAATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCGTG‑AAGTAAAACAGCGCGTAACCCAGCCAGATGGTCAGCAGAATATGCCGACGCCAGTAGCGATAGCGGGCATCAAttt < 1:505422/251‑1 (MQ=255) aagcccatgccccAAAAAAAAACGGG‑CATTTGAGCGATCGCGGACAATGCCGGAGACAAACTT‑CGAC‑ACGCCATAGGTAATGTAAAACAGGGTCGCAAACAGGCCGATACCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCGTG‑AAGTAAAACAGCGCGTAACCCAGCCAGATGGTCAGCAGAATATGCCGACGCCAGCAGCGATAGCGGGCATCAAttt < 1:77785/242‑1 (MQ=255) acccccAAAAAATAACGGG‑CATTTGAGCGATCTCTGAAAATCCCGGAGAAAAACTT‑CGAC‑ACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCGTG‑AAGTAAAACAGCGCGTAACCCAGCCAGATGGTCAGCAGAATAGGCCGACGGCAGTAGCGATAGCGGGCATCAATTTCatat < 1:147748/247‑1 (MQ=255) tCCCCATAAAATAACGGG‑CATTTGAGCGATCGCTGACAATGCCGGAGACAAACTT‑CGAC‑ACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCGGG‑AAGTCAAACAGCGCGTAACCCAGCCCGATGGTCAACAGAATATGCCGACCCCAGAAGCCGTAACGGGCACCCATTTCCTAtttt > 2:168973/1‑250 (MQ=255) ccccAAAAAATACCGGG‑AATTTGAGCGATCGCTGACAATGCCGGAGACAAACTT‑CGAC‑ACGCCATAGGTAATGTAAACCAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCGTG‑AAGTAAAACAGCGCGTAACCCAGCCAGATGGTCAGCAGAATATGCCGACGCCAGTAGCGATAGCGGGCATCAATTTCATATTTAt < 1:3940/251‑1 (MQ=255) tAAAATAACGGG‑CATTTGAGCGATCGCTGACAATGCCGGAGACAAACTT‑CGAC‑ACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCGTG‑AAGTAAAACAGCGCGTAACCCAGCCAGATGGTCAGCAGAATATGCCGACGCCAGTAGCGATAGCGGGCATCAATTTCATATTTATCAGTc > 1:225761/1‑251 (MQ=255) | AATGCAGCGCAGCCGCTGCCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAA‑CCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAAC‑GCCCATAGC‑GACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCT‑ATCCCCATAAAATAACGGG‑CATTTGAGCGATCGCTGACAATGCCGGAGACAAACTT‑CGAC‑ACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCGTG‑AAGTAAAACAGCGCGTAACCCAGCCAGATGGTCAGCAGAATATGCCGACGCCAGTAGCGATAGCGGGCATCAATTTCATATTTATCAGTC > NC_000913/3848084‑3848583 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |