Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,556,200	0	G	A	25.0%	78.6 / 12.9	48	S217F (TCC→TTC)	rtcA	RNA 3'‑terminal phosphate cyclase
Reads supporting (aligned to +/- strand):  ref base G (34/2);  new base A (0/12);  total (34/14)
Fisher's exact test for biased strand distribution p-value = 1.31e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACC‑GACGACAAAAAAGCGTTCGGTGATATTTT‑CACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGG‑TC‑GCGCGGCAGGTT‑ATGAATATTCTGT‑T‑CATGCAGGGAAAAACTCC‑CC‑GCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGCCACCACACCGCCTCCGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGAT  >  NC_000913/3555950‑3556447                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                          aaaTTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACC‑GACGACAAAAAAGCGTTCGGTGATATTTT‑CACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGG‑TC‑GCGCGGCAGGTT‑ATGAATATTCTGT‑T‑CATGCAggg                                                                                                                                                                                                                                                           <  1:173507/251‑1 (MQ=255)  aaTTCCCCCGCGCCCGCCCGCGCCATCGGTAGTACCAGTTGGTCAGCAAGCTATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCCCTGACGCGCTTTTCACC‑GACGACAAAAAAGCGTTCGGTGATATTTT‑CACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGG‑TC‑GCGCGGCAGGTT‑ATAGCTATTATGT‑G‑CATGCAggg                                                                                                                                                                                                                                                           >  2:260914/1‑250 (MQ=255)           ccgcccgccAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACC‑GACGACAAAAAAGCGTTCGGTGATATTTT‑CACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGG‑TC‑GCGCGGCAGGTT‑ATGAATATTCTGT‑T‑CATGCAGGGAAAAACtacc                                                                                                                                                                                                                                                 <  1:526245/250‑4 (MQ=255)             gcccgccAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACC‑GACGACAAAAAAGCGTTCGGTGATATTTT‑CACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGG‑TC‑GCGCGGCAGGTT‑ATGAATATTCTGT‑T‑CATGCAGGGAAAAACTCC‑CC‑g                                                                                                                                                                                                                                             >  1:429480/1‑251 (MQ=255)                    aGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACC‑GCCGACAACAACGCGTTCGGTGATATTTT‑CACTTTCGACTTCCAGCGAGACGGTATTACCAGGCCCCTGG‑TC‑GCGCGGCAGGAT‑ATTCATATTCAGT‑T‑CCTGCATGGAAAAAATCC‑CC‑ACCAgttt                                                                                                                                                                                                                                      >  2:507167/1‑249 (MQ=255)                                aGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTTCCGCAACCACCTCGGCACTGACGCGCTTTTCACC‑GACGACAAAAAAGCGTTCGGTGATATTTT‑CACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGG‑TC‑GCGCGGCAGGTT‑ATGAATATTCTGT‑T‑CATGCAGGGAAAAACTCC‑CC‑GCCAGTGTAGCGATTTCACg                                                                                                                                                                                                                          >  1:430496/1‑251 (MQ=255)                                  cACCAGTTGATCAGCAAGCTATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACC‑GACGACAAAAAAGCGTTCGGTGATATTTT‑CACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGG‑TC‑GCGCGGCAGGTT‑ATGAATATTCTGT‑T‑CATGCAGGGAAAAACTCC‑CC‑GCCAGTGTAGCGGTTTCACGCt                                                                                                                                                                                                                        >  2:500093/1‑251 (MQ=255)                                               gCAAGAGATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACC‑GACGACAAAAAAGCGTTCGGTGATATTTT‑CAATTTCGACTTCATGCGAGACGGTATTACCCGGCCCCTGG‑TC‑GCGCGGCAGGTT‑ATGAATATTCTGT‑T‑CATGCAGGGAAAAACTCC‑CC‑GCCAGTGTAGCGATTGCACGCTCACCAAccaggcg                                                                                                                                                                                                           >  2:473111/1‑245 (MQ=255)                                                     ttccccccccccccCGCCGTGCTTGCCAGGTAGTGTTCCCCCTCTTCCCCCACCGGCCCCCAAACCACCCCGGCTCTAACGCGCTTTTCCCC‑AACAACAAAAAAGCGTTCGTGGATATTTT‑CCTTTTCGACTTAAAGCGAGACGGTATTCCCCGGCCCTTGG‑TC‑GCGCGGAGGGTT‑ATAAATATTCTGT‑T‑CATGCAGGAAAAACCTCC‑CC‑GCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGca                                                                                                                                                                                                      <  1:265070/246‑1 (MQ=255)                                                      atTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACC‑GACGACAACACAGCGTTCGGTGATATTTT‑CACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGG‑TC‑GCGCGGCAGGTT‑ATGAATATTCTGT‑T‑CATGCAGGGACAACCTCC‑CC‑GCCCGTGTAGCGATTTCACGCGCAGCAACCTGGCGCGGcacc                                                                                                                                                                                                    >  2:545883/1‑250 (MQ=255)                                                                      cgTGCTTGCAAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACC‑GACGACAAAAAAGCGTTCGGTGATATTTT‑CACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGG‑TC‑GCGCGGCAGGTT‑ATGAATATTCTGT‑T‑CATGCAGGGACAAACTCC‑CC‑GCCAGTGTAGCGCTTTCACGCTCAGCAACATGGCGCGGACCACCAGCTAATAGaaatt                                                                                                                                                                                    >  2:128453/1‑248 (MQ=255)                                                                                aGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACC‑GACGACAAAAAAGCGTTCGGTGATATTTT‑CACTTTCGACTTCAAGCGAGACGGTATTACCAGGCCCCTGG‑TC‑GCGCGGCAGGTT‑ATGAATATTCTGT‑T‑CATGCAGGGAAAAACTCC‑CC‑GCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCAt                                                                                                                                                                          >  2:406192/1‑251 (MQ=255)                                                                                  gTAGCGTTTCACCTCTTTCACCAACTGGGCCGCAACCACCTCGGCACTGACGCGCTTTTCACC‑GACGACAAACAAGCGTTCGGGGATATTTT‑CACTTTCGACTTCAAGCGAGACGGAATTACCCGGCCCCTGG‑TC‑GCGCGGCAGGTT‑ATGAATATTCTGT‑T‑CATGCAGGGAAAAACTCC‑CC‑GCCAGTGTAGCGATTTCACGCTAAGCAAAATGACGCGGCACCCCAGCTAATAGCACTTCTCCaaccctct                                                                                                                                                                        >  2:430405/1‑244 (MQ=255)                                                                                                 ttccACCAACTGTCCCGCACCCCCCTCGGCCCTGACGC‑CTTTTCCCCCGACGAAAAAAAAGGGTT‑GGGGATTTTTTTCCCTTTCGACTTAAGGGGGGAGGTTTTTCCCCGCCCCCTGG‑CC‑GCGCGGCGGTTT‑ATAAATTTTCTTT‑C‑CATGCAGGAAAAACCCCC‑CC‑GCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTccc                                                                                                                                                           <  1:132381/246‑1 (MQ=255)                                                                                                  tcccccactgggcccGCAACCCCCGCGGCCCTGCCGTCCTTTTCCCC‑GACAAAAAAAAAGCGTCCGGTTATATTTC‑CTCTTCCGACTTCAAGCGAGACGGTATTCCCCGCCCCCTGG‑TC‑GGGCGGCAGGTT‑ATAAATATT‑TGT‑TTCATGCAGGAAAAACCCCC‑CC‑GCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTccc                                                                                                                                                           <  1:386846/235‑1 (MQ=255)                                                                                                     aCCAACTGTGCCGCAACAACCTCGGCACTGACGCGCTTTTCACC‑GACGACAAAACAGCGTTCGGTGATATTTT‑CACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGG‑TC‑GCGCGGCAGGTT‑ATGAATATTCTGT‑T‑CATGCAGGGAAAAACTCC‑CC‑GCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGATAATAGACCTTCTCCACGCATCTGCACAAAGTTCCCGCGCTc                                                                                                                                                     >  2:486546/1‑251 (MQ=255)                                                                                                       cAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACC‑GACGACAAAAAAGCGTTCGGTGATATTTT‑CACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGG‑TC‑GCGCGGCAGGTT‑ATGAATATTCTGT‑T‑CATGCAGGGAAAAACTCC‑CC‑GCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCCCAATGTTCCCGCGCCCGc                                                                                                                                                   >  2:532563/1‑251 (MQ=255)                                                                                                              gccgaacccaccGCGGCACGGAGGCGTTTTCCACC‑GACGAAAAAAAAGGGTTGGGGAATTTTTC‑CATTTGCGATTTAAAGGGAGACGGTATTCCCCGCCCCGTGG‑CC‑GCGGGGAAGGTT‑ATAAATATTCTGT‑T‑CATGAAGGAAAAAACCCC‑CC‑GCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTg                                                                                                                                            <  1:243374/244‑1 (MQ=255)                                                                                                                   aaCCACCTCGGCACTGACGCGCTTTTCACC‑GACGACAAAAAAGCGTTCGGTGATATTTT‑CACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGG‑TC‑GCGCGGCAGGTT‑ATGAATATTCTGT‑T‑CATGCAGGGAAAAACTCC‑CC‑GCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAgg                                                                                                                                       >  1:147843/1‑251 (MQ=255)                                                                                                                           cGGCACTGACGCGCTTTTCACC‑GACGACAAAAAAGCGTTCGGTGATATTTT‑CACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGG‑TC‑GCGCGGCAGGTT‑ATGAATATTCTGT‑T‑CATGCAGGGAAAAACTCC‑CC‑GCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGAGTTaaa                                                                                                                                >  2:38798/1‑250 (MQ=255)                                                                                                                            ggCACTGCCGCGTTTTTCCCC‑GACGAAAAAAAAGCGTTGGGTGTTTTTTT‑CCCTTTCGACTTCAAGCGAGACGTTTTTACCCGCCCCCGGG‑TC‑GCGCGGCAGTTT‑ATGAATATTCTTT‑T‑CATGCAGGGAAAACCTCC‑CC‑GCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACg                                                                                                                              <  1:450355/251‑1 (MQ=255)                                                                                                                             gCACTGACGCGCTTTTCACC‑GACGACAAAAAAGCGTTCGGTGATATTTT‑CACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGG‑TC‑GCGCGGCAGGTT‑ATGAATATTCTGT‑T‑CATGCAGGGAAAAACTCC‑CC‑GCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACg                                                                                                                              >  2:364816/1‑250 (MQ=255)                                                                                                                                cTGACGCGCTTTTCACC‑GACGACAAAAAAGCGTTCGGTGATATTTT‑CACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGG‑TC‑GCGCGGCAGGTT‑ATGAATATTCTGT‑T‑CATGCAGGGAAAAACTCC‑CC‑GCCAGAGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGACCTTCTCCACGCCTCTGCACCATGTTCCCGCGCCCGCCAAGTTGCAAGGAGTTAAACGATGc                                                                                                                          >  2:486111/1‑251 (MQ=255)                                                                                                                                     gcgcTTTTCACC‑GACGACAACAAAGCGTTCGGTGATATTTT‑CACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGG‑TC‑GCGCGGCAGGTT‑ATGAATATTCTGT‑T‑CATGCAGGGAAAAACTCC‑CC‑GCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCACGTTGCCAGGTGTTAAAAGGTGCCACgg                                                                                                                     >  2:544165/1‑251 (MQ=255)                                                                                                                                        cTTTTCACC‑GACGACAAAAAAGCGTTCGGTGATATTTT‑CACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGG‑TC‑GCGCGGCAGGTT‑ATGAATATTCTGT‑T‑CATGCAGGGAAAAACTCC‑CC‑GCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCg                                                                                                                  >  1:196481/1‑251 (MQ=255)                                                                                                                                                   ccgacCAAACAGCGATCGGTGATATTTT‑CACTTTCGACTTCAAGCGAGAAGGTATTACCCGGCCCCTGG‑AC‑GCGCGGCAGGTT‑ATGACTATTCTGT‑T‑CATGCAGGGAAAAACTCC‑CC‑GCAAGTGTAGCGCTTTCACGCTCAGCAACATGGCGCGGCACACCCGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCCAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGCCTTCCg                                                                                                         >  2:505915/2‑250 (MQ=255)                                                                                                                                                    cgacAAAAGAGCGTTCGGTGAAATTTT‑CACTTTCGACTTCAAGCGAGACGGTATTCCACGGCCCCTTG‑TC‑GCGCGGCAGGTT‑ATGAGTATTCTGT‑T‑CATGCAGGGAAAAAATCC‑CC‑GCCAGTGTAGCGATTTCACGCTCCGCCACATGGCGCGGCACACCAGCTAATAGAAATTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGtt                                                                                                       >  2:5220/1‑251 (MQ=255)                                                                                                                                                       cAAAAAAGCGTTCGGTGATATTTT‑CACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGG‑TC‑GCGTGGCAGGTT‑ATGAATATTCTGT‑T‑CATGCAGGGAAAAACTCC‑CC‑GCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGcc                                                                                                    >  1:176842/1‑251 (MQ=255)                                                                                                                                                                      tGATATTTT‑CCCTTTCGACTTCCAGCGCGACGGTATTACCCGGCCCCTGG‑TC‑GCGCGGCAGGTT‑ATGAATATTCTGT‑T‑CATGCAGGGAAAAACTCC‑CC‑GACAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCCCAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCAAGACTTCCGTTGCCACCACACCGCCCCCg                                                                                     >  2:324158/1‑251 (MQ=255)                                                                                                                                                                      gagatattttccttcccgaatgaaaGCGAGACGGTTTTCCCCGGCCC‑TGGCTC‑GCGCGGAAGTTT‑ATAAATTTTCTTT‑G‑CATGAAGGAAAAACCCCC‑CC‑GCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGCCACCACACCGCCTcc                                                                                      <  1:33126/228‑1 (MQ=255)                                                                                                                                                                         atttttcccttttCGTCTCCAAGCGAGACGGTATT‑CCCCGCCCCTGG‑TCCGCGGGTCTGGTT‑ATAAATTTTCT‑T‑T‑TATGGAAGAAAAAACCCC‑CCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGCCACCACACCGCCTCCGGc                                                                                   <  1:39584/241‑1 (MQ=255)                                                                                                                                                                          atTTT‑CACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGG‑TC‑GCGCGGCAGGTT‑ATGAATATTCTGT‑T‑CATGCAGGGAAAAACTCC‑CC‑GCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGCCACCACACCGCCTCCGGc                                                                                   >  2:116833/1‑249 (MQ=255)                                                                                                                                                                                      cgactaaaaGCGAGACGGTATCCCCCGCCCCGGGG‑GC‑GCGGGGCGGGTT‑ATGAGTATCCTGT‑T‑ACGGTAGGAAAAACCCCC‑CC‑GCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGCCACCACACCGCCTCCGGCAGGGTAAAAAcc                                                                       <  1:128453/243‑1 (MQ=255)                                                                                                                                                                                      cGACTTCAAGCGAGACGGTATTACCCGGCCCCTGG‑TC‑GCGCGGCAGGTT‑ATGAATATTCTGT‑T‑CATGCAGGGAAAAACTCC‑CC‑GCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGCGACTTCCGTTGCCACCACACCGCCTCCGGCAGGGaaaaaaacg                                                                      >  2:72675/1‑248 (MQ=255)                                                                                                                                                                                      cGACTTCAAGCGAGACGGTATTACCCGGCCCCTGG‑TC‑GCGCGGCAGGTT‑ATGAATATTCTGT‑T‑CATGCAGGGAAAAACTCC‑CC‑GCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGCCACCACACCGCCTCCGGCAGGGTAAAAACCg                                                                      >  1:297755/1‑251 (MQ=255)                                                                                                                                                                                          tcaaaggggggcgttttcccccGGCCCCGGG‑CC‑GCGGGGCGGGTT‑ATAATTATTTTGT‑T‑CAGGCAGGAAAAACCCCC‑CC‑GCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGCCACCACACCGCCTCCGGCAGGGTAAAAACCGTg                                                                    <  1:116833/230‑1 (MQ=255)                                                                                                                                                                                             aaGCGAGAGGTTATTCCCGGGCCCCGGG‑TC‑GCGCGGCGTGTT‑TTAAATAT‑CTTTCT‑CATGCAGGAAAAAACTCC‑CC‑GCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGCCACCACACCGCCTCCGGCAGGGTAAAAACCGTGGCGTa                                                               <  1:371359/251‑1 (MQ=255)                                                                                                                                                                                                  atgcggttttccccGCCCCGTGG‑GC‑GCGCGG‑AGGTTTATAAATATTTTGT‑T‑CAGGCAGAAAAAAACCCC‑CC‑GCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGCCACCACACCGCCTCCGGCAGGGTAAAAACCGTGGCGTAACAGc                                                          <  1:532563/240‑1 (MQ=255)                                                                                                                                                                                                     cGGTATTACCCGGCCCCTGG‑TC‑GCGCGGCAGGTT‑ATGAATATTCTGT‑T‑CATGCAGGGAAAAACTCC‑CC‑GCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGCCACCACACCGCCTCCGGCAGGGTAAAAACCGTGGCGTAACAGCg                                                         >  1:309332/1‑249 (MQ=255)                                                                                                                                                                                                         aTTACCCGGCCCCTGG‑TC‑GCGCGGCAGGTT‑ATGAATATTCTGT‑T‑CATGCAGGGAAAAACTCC‑CC‑GCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAACCGATGCCACGGGCGAGACTTCCGTTGCCTCCACACAGCCTCCGGCAGGGTCAAAACCGTGGCGAAACACCGTGGttt                                                   >  2:302476/1‑251 (MQ=255)                                                                                                                                                                                                                ggCCCCTGG‑TC‑GCGCGGCAGGTT‑ATGAATATTCCGT‑T‑AATGCAGGGAAACACTCC‑CC‑GCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGACCTTCTCCACGCATCTGCACCATGTTCCCGCGCTCGCCAAGTTGCCAGGTGTTAAACGATGCCACGGGCGCGACTTCCGTTGCCACCACACCGCCTCCGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCtgatg                                            >  2:273927/1‑251 (MQ=255)                                                                                                                                                                                                                      tGG‑TC‑GCGCGGCAGGTT‑ATGAATATTCTGT‑T‑CATGCAGGGAAAAACTCC‑CC‑GCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGCCACCACACCGCCTCCGGCAGGGTAAAAACCGCGGCGTAACAGCGTGGTTTGCTGATGAATTcc                                      >  1:365929/1‑251 (MQ=255)                                                                                                                                                                                                                       gg‑TC‑GCGCGGCAGGTT‑ATGAATATTCTGT‑T‑CATGCAGGGAAAAACTCC‑CC‑GCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGCCACCACACCGCCTCCGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCt                                     >  1:152085/1‑251 (MQ=255)                                                                                                                                                                                                                           c‑gcgcgGCAGGTT‑ATGAATATTCTGT‑T‑CATGCAGGGAAAAACTCC‑CC‑GCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGCCACCACACCGCCTCCGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTAtt                                  >  1:342636/1‑251 (MQ=255)                                                                                                                                                                                                                                 ggCAGGTT‑ATGAATATTCTGT‑T‑CATGCAGGGAAAAACTCC‑CC‑GCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACATCCGTTGCCACCACACCGCCTCCGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGc                             >  1:97369/1‑251 (MQ=255)                                                                                                                                                                                                                                      gTT‑ATGAATATTCTGT‑T‑CATGCAGGGAAAAACTCC‑CC‑GCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGCCACCACACCGCCTCCGGCAGGGTAACAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCcagc                         >  2:326627/1‑250 (MQ=255)                                                                                                                                                                                                                                            aaaTATTTTGT‑T‑CAGGAAGGAAAAACCCCC‑CC‑GCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCGCGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGCCACCACACCGCCTCCGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGc                   <  1:364816/250‑1 (MQ=255)                                                                                                                                                                                                                                                     gT‑T‑CATTCAGGGAAAAACTCC‑CC‑GCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATTCCACGGGCGAGACTTCCGTTGCCACCACACCGCCTCCGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCAcc          >  1:433776/1‑251 (MQ=255)                                                                                                                                                                                                                                                       gtctcggtggggAAAAACTCCACC‑GCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGAATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGCCACCACACCGCCTCCGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAg              <  1:507167/239‑1 (MQ=255)                                                                                                                                                                                                                                                            ggCAGGAAAAACCCCC‑CC‑GCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGCCACCACACCGCCTCCGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCcggcg     <  1:616/250‑1 (MQ=255)                                                                                                                                                                                                                                                               aGGGAAAAACTCC‑CC‑GCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGCCACCACACCGCCTCCGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCCGCAGCGGCTCCAGCACCCGGCGGAt  >  1:476021/1‑251 (MQ=255)                                                                                                                                                                                                                                                                gggAAAAACCCC‑CCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGCCACCACACCGCCTCCGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGAt  <  1:464647/251‑1 (MQ=255)                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                          AAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACC‑GACGACAAAAAAGCGTTCGGTGATATTTT‑CACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGG‑TC‑GCGCGGCAGGTT‑ATGAATATTCTGT‑T‑CATGCAGGGAAAAACTCC‑CC‑GCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGCCACCACACCGCCTCCGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGAT  >  NC_000913/3555950‑3556447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑