Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913580,1190AG21.9% 56.2 / 11.7 32intergenic (+33/+132)ybcW/ylcIuncharacterized protein YbcW/DUF3950 domain‑containing protein YlcI
Reads supporting (aligned to +/- strand):  ref base A (13/12);  new base G (4/3);  total (17/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01
Rejected as consensus: Frequency below/above cutoff threshold.

AGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAA‑CTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCTCAAGAGCG  >  NC_000913/579871‑580365
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      
aGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAA‑CTGCTAATCATTCAAACTACTTAAcc                                                                                                                                                                                                                                                      <  2:169826/251‑1 (MQ=255)
           gAATAAAGAAAAATCTGCTGTAGTGCCCTCCGTTGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTGCTATGAGTTACCCTGATGTTGTAATTGGATGTATAGAACATAAGGTTTCTCTGGAAGCATTCAGGGTAATTGAGGCAGCGTTGGTGAAGCACGATAATAATAGGAAGGATTATGCCCTGGTGGTTGTCTGATCACCATAA‑CTGCTAATCATGCAAACTACTTAACCTGTGACagag                                                                                                                                                                                                                                            <  2:446563/250‑1 (MQ=255)
               aaaGAACAGTCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAACACCATTTCTATGAGTTAAACTGATGCTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCCGGGAAATTGAGGCAGCGTTGGTGAAGCACGCTAAGAATATGAAGGATTATTCCCTTGGAGTGGACTGATCAA‑ATAAACAGCTCATCATTAAAACTACTTAACCTGTGACatagtcaa                                                                                                                                                                                                                                        >  2:331339/1‑243 (MQ=255)
                aaGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGAAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGGGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAA‑CTGCTAATCATTCAAACTACTTAACCTGTGAAAGAACCAac                                                                                                                                                                                                                                       >  2:17033/1‑250 (MQ=255)
                         ctgctgATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAA‑CTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTctg                                                                                                                                                                                                                             >  1:203138/1‑251 (MQ=255)
                           gctgATGATCCCTCCGTGTAGCTGATTCGTGTAAAAAATATGTTTAATAGCACCATTTCTATGAGTTCCCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAA‑CTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTctgt                                                                                                                                                                                                                            <  2:415015/250‑1 (MQ=255)
                                             gATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAA‑CTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTTTCACTGTCAGGAAAGTgtt                                                                                                                                                                                                          >  2:94048/1‑248 (MQ=255)
                                               tCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAA‑CTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTaa                                                                                                                                                                                                        >  2:227785/1‑250 (MQ=255)
                                                  taTTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAA‑CTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTaaa                                                                                                                                                                                                       <  1:197493/247‑1 (MQ=255)
                                                  gATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTGCCTGGTGGTTGACTGATCACCATAA‑CTGCTAATCATTCAAACTACTTAACCTGTGCCAGAGc                                                                                                                                                                                                                                           >  2:120734/1‑212 (MQ=255)
                                                  gATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAA‑CTGCTAATCATTCAAACTACTTAACCTGTGACAGAGc                                                                                                                                                                                                                                           <  1:120734/212‑1 (MQ=255)
                                                              aaaTATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCCTGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAACGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAA‑CTGATAATCATTCAAACTACTTAACCTGTGACAGAGGCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAAtt                                                                                                                                                                                         >  1:512955/1‑250 (MQ=255)
                                                               aaTATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAA‑CTGCTAATCATTCAAACACCTTAACCTGTGACAAAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTAc                                                                                                                                                                                       >  2:419042/1‑251 (MQ=255)
                                                                 tatGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAA‑CTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTg                                                                                                                                                                                     >  1:232544/1‑251 (MQ=255)
                                                                 tatGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGGCTGATCACCATAA‑CTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCAAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTg                                                                                                                                                                                     >  1:175251/1‑251 (MQ=255)
                                                                                          aGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAA‑CTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAAtt                                                                                                                                                            >  2:427069/1‑251 (MQ=255)
                                                                                               cccTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGGGAAGAACGATAATAATATGAAGGATTAATCCCTGGTGGTTGACTGATCACCATAA‑CTGCTAATCATTCAACCTACTTAACATGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACa                                                                                                                                                        >  2:330597/1‑250 (MQ=255)
                                                                                                   gATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAA‑CTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTAAAATATc                                                                                                                                                   <  1:94048/251‑1 (MQ=255)
                                                                                                                cATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAA‑CTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAg                                                                                                                                      <  2:327506/251‑1 (MQ=255)
                                                                                                                cATGTATAGAACATAAGGGGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGAGTATTCCCTGGTGGTTGACTGATCACCATAA‑CTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCACTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAg                                                                                                                                      <  2:271704/251‑1 (MQ=255)
                                                                                                                          aCATAAGGTGTCTCTGAAAGCATTCAGAGAAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAA‑CTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAAcgc                                                                                                                            >  1:192032/1‑251 (MQ=255)
                                                                                                                            aTAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACTATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAA‑CTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCgg                                                                                                                          <  2:153686/251‑1 (MQ=255)
                                                                                                                                            atcATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTAGTTCCTGGTGGTTGACTGATCTCCATAA‑CTGCTAATCATTCAAACTACTTAACCTGTTACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTtcat                                                                                                          <  2:275380/249‑1 (MQ=255)
                                                                                                                                                                          gAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAA‑CTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGAGGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCtttt                                                                            <  1:419042/251‑1 (MQ=255)
                                                                                                                                                                                       aataTGAAGGATTTTTCCCTGGTGGTTGACTGATCACCATAA‑CTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGCCt                                                               >  1:407804/1‑251 (MQ=255)
                                                                                                                                                                                        atatGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAA‑CTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCGCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGCCTc                                                              <  2:1640/251‑1 (MQ=255)
                                                                                                                                                                                                              ggtTGGCTGATCACCATAA‑CTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTGCATTCTTCATCCCTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGTCTCCGACGGCAGGCTTCAATGAccc                                        <  2:175251/251‑1 (MQ=255)
                                                                                                                                                                                                                                a‑CTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTATTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGTCTCCGACGGCAGGCt                                                  >  1:267098/1‑223 (MQ=255)
                                                                                                                                                                                                                                a‑CTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTATTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCGCTCTTTTCTGACGTTAGTCTCCGACGGCAGGCt                                                  <  2:267098/223‑1 (MQ=255)
                                                                                                                                                                                                                                    gCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGAc                    <  2:203138/250‑1 (MQ=255)
                                                                                                                                                                                                                                              cAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCTc         >  1:247539/1‑251 (MQ=255)
                                                                                                                                                                                                                                              cAAACTAATTAACCTGTGACAGAGCCAACACTCAGGCTGTCACTGTCAGGAAAGTGGTAACACTGCAACTCAATTACTGCAATGCCATCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCATGCTTAAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCTc         >  2:404008/1‑251 (MQ=255)
                                                                                                                                                                                                                                                       gaaCCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCTCAAGAGCg  <  2:232544/248‑1 (MQ=255)
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      
AGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAA‑CTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCTCAAGAGCG  >  NC_000913/579871‑580365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑