Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,293,326	IS1 (–) +9 bp	intergenic (‑404/‑193)	hns ← / → tdk	DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	257908 =	NA (NA)	34 (0.650)	30/492	0.8	91.6%	noncoding (768/768 nt)	IS1	repeat region
	?	NC_000913	= 1293334	3 (0.060)					intergenic (‑412/‑193)	hns/tdk	DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
*	?	NC_000913	1293326 =	3 (0.060)	47 (0.900)	44/492	0.2	93.8%	intergenic (‑404/‑201)	hns/tdk	DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
	?	NC_000913	= 1979270	NA (NA)					noncoding (1/768 nt)	IS1	repeat region

GCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTC  >  NC_000913/1979037‑1979286                                                                                                                                                                                                                                          |                 gCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGGGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCagttcgcgatgataa  <  2:114220/250‑15 (MQ=2)                                                                                                                                                                                                                                          |                 GCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTC  >  NC_000913/1979037‑1979286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.