Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,560,455	+G	intergenic (+589/+167)	rtcR → / ← glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,560,455	1	.	G	100.0%	197.9 / NA	55	intergenic (+589/+167)	rtcR/glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (24/31);  total (24/31)

TTTACCATCAACGTGTTAGCAACGT‑TGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGA‑TTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGG‑TCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAC‑CGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCG  >  NC_000913/3560212‑3560685                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                         tttACCATCAACGTGTTAGCAACGT‑TGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTCCCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGA‑TTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGcgcag                                                                                                                                                                                                                                       <  1:236161/248‑5 (MQ=255)   tacaatcagcgggtTAGCAACGTGTG‑GAGTGTTGGTGACAAGGCGAAAATTGCTGTGAGTGAGCTGGGGCTGCGCTACCGCTTCCGGCGTGGGGCCGATAACGATAAACGGCGTCGGGCTATTGGGGA‑TTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATc                                                                                                                                                                                                                                   <  2:125171/238‑1 (MQ=255)    aCCATCAACGTGTTAGCAACTT‑TGAGTTTTTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGA‑TTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCa                                                                                                                                                                                                                                  <  1:419508/250‑1 (MQ=255)     ccaacaccGTGTTAGCAACGT‑TGAGATTGTTGGTGACAATCCGCAATTTGCTGTGATTGAGCAGTGCGTCCGCTACCGCTTCCGGCGCGCTGCCGATATCGATACACAGCGTCGAGCCATTGGGGA‑TTGGCTCCGCCACGTTGCTGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGAGCCACGGCGTGTTAACCGAACCGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAgg                                                                                                                                                                                                                                <  1:469455/244‑1 (MQ=255)       aTCAACGTGTTAGCAACGT‑TGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGA‑TTTGCTACGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGtt                                                                                                                                                                                                                              >  2:355374/1‑251 (MQ=255)        tCAACGTGTTAGCAACGT‑TGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGA‑TTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCCGCGCCGCAGCGCCATGATGGCGCAGGATCAGGttt                                                                                                                                                                                                                             >  2:156461/1‑251 (MQ=255)            cGTGTTAGCAACGT‑TGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGA‑TTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCt                                                                                                                                                                                                                         <  1:243105/251‑1 (MQ=255)             gtgtTAGCAACGT‑TGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGA‑TTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTc                                                                                                                                                                                                                        >  1:93170/1‑251 (MQ=255)                  aGCAACGT‑TGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGA‑TTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGTCGCAGGATCAGGTTTTGCTCCGccc                                                                                                                                                                                                                   >  1:179540/1‑250 (MQ=255)                            gagaTTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGA‑TTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCCTGCTGGCGCAGGATCCGGTTTTGCTCCGCCAGCTCATTg                                                                                                                                                                                                           >  2:347638/1‑250 (MQ=255)                               aTTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGA‑TTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGCACTGGAATGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTgcggg                                                                                                                                                                                                       >  2:233906/1‑247 (MQ=255)                                       tGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGA‑TTTGCTCCGCCACTTTGAGGGCGATGCGCTCTTTTTCTTCGGTATGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGCAGGCAGCGCCGCAGCGCCATGATGGCGCAGGAACAGGTTTTGATCCGCCAGCCCATTGAGG‑Tagcgccg                                                                                                                                                                                               >  2:373265/1‑247 (MQ=255)                                       tGACAATGCGCAAATTGCTGTGATTGAGCAGGGCGTGCGATACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCCTTGGGGA‑TTTGCTCCGCCACTTCGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTACCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCCGCGCCATGATGGCGCAGGATCATGTTTTGCTCCGCCAGCTCAT‑GAGGGTCgcggcg                                                                                                                                                                                               >  2:263970/1‑250 (MQ=255)                                                           ggATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCAATAAACAGCGTCGAGCCATTGGGGA‑TTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGG‑TCGCGGCGAATAGTCTGCGGGCTGACGga                                                                                                                                                                          <  1:200101/250‑1 (MQ=255)                                                            gATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGA‑TTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGG‑TCGCGGCGAATAGTCTGCGGGCTGACgg                                                                                                                                                                           >  1:205292/1‑249 (MQ=255)                                                                                       ccGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGA‑TTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGCTCGTGCCACGGCGTGTTAACCGCACTGGAAGGCAGAGCCGCAGCGCCATGATGGCGCAGGCTCAGGTTTTGCTCCGCCCGCTCATTGGGG‑TCGGGGCGAATAGTCTGAGGGGTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTg                                                                                                                                              >  2:320456/1‑251 (MQ=255)                                                                                           ggtggtgCCGATATCGATAAACAGCGTCGAGCCATTGGGGA‑TTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGG‑TCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACAt                                                                                                                                          <  1:324521/250‑1 (MQ=255)                                                                                               gtgCCGATCTCGATAAACAGCGTCGAGCCATTGGGGA‑TTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGG‑TCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAcc                                                                                                                                      <  1:431887/251‑1 (MQ=255)                                                                                                   cGATATCGATAAACAGCGTCGAGCCATTGGGGA‑TTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGTTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGG‑TCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgc                                                                                                                                  >  1:363557/1‑251 (MQ=255)                                                                                                         cGATAAACAGCGTGGAGCCATTGGGGA‑TTTGCCCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGG‑TCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTa                                                                                                                            <  1:210637/251‑1 (MQ=255)                                                                                                             aaaCAGCGTCGCGCCATTGGGGA‑TTTGCTCCGCCACTTTGAGGGCGATGCGCTCTTTTTCTTCGGTCTGGGCGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGGAGGCGGCGCCGCAGCGCCATGCTGGCGCAGGATCAGGTTTTACTCCGCCCGCTCATTGAGG‑TCGCGGCGACTACTCTGCGGGCTGGCGGTGACATGCTCTAACAGCTATTCGGATCTGCTCTAACCCTGCTGTTTAAcc                                                                                                                         >  2:529087/1‑250 (MQ=255)                                                                                                                ccgtggtcgtcatgtGGGGT‑TTTTCTCCGCCACCTGGCGGGTGATGAGCTCTTTTTTTGCTGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGATG‑TCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTGCGGTACTGACATAACCCTTCTGTTTAACCAGtg                                                                                                                     <  2:17354/237‑2 (MQ=255)                                                                                                                  ggctgggttcagtGGGGAGTTTGCGCGGCCACTTTGGGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGACCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGG‑TCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTc                                                                                                                    <  2:420356/239‑1 (MQ=255)                                                                                                                       gAGCCATTGGGGA‑TTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGG‑TCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataat                                                                                                              <  1:56180/251‑1 (MQ=255)                                                                                                                          cctttGGGGT‑TTTGCCCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTGCGTGCTGGGTGGCCT‑TGCGTTCGTGCCACGGCGTGTTACCCGAACTGGAAGGCTGCGCCGCACCGCCATGATGGCGCAGGATCAGTTTTTGCTCCGCCAGCTCATTGAGG‑TCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAc‑c                                                                                                          <  1:233512/248‑1 (MQ=255)                                                                                                                          ccATTGGGGA‑TTTGCTCCTCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGG‑TCGCGGCGAATAGTCTGCGGGCTGACGGAGaaaatccccaatgg                                                                                                                                                           <  2:61781/203‑12 (MQ=255)                                                                                                                          ccATTGGGGA‑TTTGCTCCTCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGG‑TCGCGGCGAATAGTCTGCGGGCTGACGGAGaaaatccccaatgg                                                                                                                                                           >  1:61781/1‑192 (MQ=255)                                                                                                                          ccATTGGGGA‑TTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGG‑TCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAc‑c                                                                                                          <  1:363787/251‑1 (MQ=255)                                                                                                                                       tGCTACGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGG‑TCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAC‑CGTTGTGACGtt                                                                                               <  1:355374/250‑1 (MQ=255)                                                                                                                                        gccccgccgCTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGG‑TCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAC‑CGTTGTGACGTtg                                                                                              <  1:389998/247‑1 (MQ=255)                                                                                                                                         cagcggcattgtgtgggCGGTGCTCTTTTTGTCTTCGGTCTGG‑TGGGCTCTGCGCTCGGCCCAGGGCGTGTTAACTGACTTTGAAGGCAGCCCCGCACCGCCAGGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGG‑TCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAC‑CGTTGTGACGTtgtg                                                                                            <  2:395182/237‑1 (MQ=255)                                                                                                                                            cgccccgttGCGGGCGATGCGCTCTTTTCCGTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGG‑TCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAC‑CGTTGTGACGTTGtgttt                                                                                         <  2:214480/244‑1 (MQ=255)                                                                                                                                             gCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCCGCTCATTGAGG‑TCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGTTAATAA‑CGTTGTGACGTTGtgtttg                                                                                        >  2:130685/1‑251 (MQ=255)                                                                                                                                                      cGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCCGGATCAGGTTTTGCTCCGCCAGCTCATTGAAG‑TCGCGGCGAATAGTCTGAGGGCTGACGCAGCAATGCTCTACCAGCTCTTCGGAACTGACATAAACCTGCTGTTTAACCAGTTAGATAATACACGT‑GTGACGTtggtatgatttaatttt                                                                               >  2:425073/1‑236 (MQ=255)                                                                                                                                                      cGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGG‑TCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAACCTGCTGTTTAACCAGTTCGATAATAC‑CGTTGTGACGTTGTGTTTGTTTCAttt                                                                                >  1:117499/1‑250 (MQ=255)                                                                                                                                                         gCGATGCGCTCGTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTTTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGG‑TCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAC‑CGTTGTGACGTTGTGTTTGTTTCATTTATaa                                                                            <  1:181373/251‑1 (MQ=255)                                                                                                                                                          cgttGCGCTCTTTTTCTGCGGTCTGGGTGGCCT‑TGCGATCGTTCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGG‑TCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAC‑CGTTGTGACGTTGTGTTTGTTTCATTTATaaa                                                                           <  2:105605/248‑1 (MQ=255)                                                                                                                                                          cggggcggttctgttcttcGGGCTGGGTGGCCT‑TGCGAACGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCATGTTTTGCTCCGCCAGCTCATTGAGG‑TCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTTTTTATCCAGTTCGATAATAC‑CGTTGTGACGTTGTGTTTGTTTCATTTATaaa                                                                           <  2:53739/238‑1 (MQ=255)                                                                                                                                                          cGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGG‑TCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAC‑CGTTGTGACGTTGTGTTTGTTTCATTTATaaa                                                                           <  1:481487/251‑1 (MQ=255)                                                                                                                                                           gATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCTGGATCAGGTTTTGCTCCGCCAGCTCATTGAGG‑TCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTTCCAGCTCTTCGGTACTGCCATAACCCTGCTGTTTAACCAGGTCGATAATTC‑CGTTGTGACGTTGTGTTTGTTTCATTTATAAAt                                                                          >  2:43189/1‑251 (MQ=255)                                                                                                                                                              gcgcTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGG‑TCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAC‑CGTTGTGACGTTGTGTTTGTTTCATTTATAAATcc                                                                        <  1:544127/250‑1 (MQ=255)                                                                                                                                                                           tcGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGG‑TCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAC‑CGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttt                                                            <  1:320456/249‑1 (MQ=255)                                                                                                                                                                           tcGGGCTGGGTGGCCT‑GGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCATCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGG‑TCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAC‑CGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAtttt                                                           <  2:73728/250‑1 (MQ=255)                                                                                                                                                                                cTGGGTGGCCT‑TTCGATCGTGCCACGGCGTGTTAACCGAACTGGCAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGATTTGCTCCGCCAGCTCATTGAGG‑TCGCGGCGAATAGTCTGCGGGCTGACAGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGATCGATAATCC‑CGTTGTGACGTTGTGTTTGTTTCTTTTATAAATCCCTGGAATTAttttcgtttt                                                     >  1:80560/1‑251 (MQ=255)                                                                                                                                                                                 tGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCCGCTCATTGAGG‑TCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAC‑CGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGTATTAttttcgttttc                                                    >  1:527396/1‑251 (MQ=255)                                                                                                                                                                                  gggTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCCCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGG‑TCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAC‑CGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgtttt                                                     <  1:74919/249‑1 (MQ=255)                                                                                                                                                                                      ggCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCCGCGCCGCAACGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGG‑TCGCGGCGAATAGTCTGCGGGCTGACGGAGAACTGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAC‑AGTTGTGACGTTGTGTTTGTTTCATTTTTAAATCCCTGGAATTATTTTCGTTATCgcgc                                                >  2:230480/1‑250 (MQ=255)                                                                                                                                                                                         cT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGG‑TCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGCCATAACCCTGCTGTTTAACCAGTTCGATAATAC‑CGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTg                                            >  1:522930/1‑251 (MQ=255)                                                                                                                                                                                                           gCGTGTTAACCGAACTGTAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCCGCTCATTGAGG‑TCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTCACCAGTTCGATAATAC‑CGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGc                           >  2:508011/1‑251 (MQ=255)                                                                                                                                                                                                             gtgtTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGG‑TCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGATGTTTAACCAGTTCGCTAATAC‑CGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAAAAAAAGcc                          >  1:248644/1‑250 (MQ=255)                                                                                                                                                                                                               gtTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGG‑TCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAC‑CGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATc                       >  1:306297/1‑251 (MQ=255)                                                                                                                                                                                                               gtTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGG‑TCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAC‑CGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTGCGCGCATTGAGCGAATCAACAAAAGCCAt                        <  1:156461/250‑1 (MQ=255)                                                                                                                                                                                                                       aaCTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGG‑TCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAC‑CGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAAcc               <  1:83857/251‑1 (MQ=255)                                                                                                                                                                                                                           ggAAGGCCGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGG‑TCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAC‑CGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCAc            <  1:43189/250‑1 (MQ=255)                                                                                                                                                                                                                            gAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGG‑TCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAC‑CGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACgg          <  1:276439/251‑1 (MQ=255)                                                                                                                                                                                                                                    cgccgcAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGG‑TCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAC‑CGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCg  <  1:288187/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                         TTTACCATCAACGTGTTAGCAACGT‑TGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGA‑TTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCT‑TGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGG‑TCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAC‑CGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCG  >  NC_000913/3560212‑3560685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑