Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,088,010	2 bp→TT	intergenic (+150/‑273)	metK → / → galP	methionine adenosyltransferase/galactose:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,088,010	0	C	T	96.8%	102.7 / ‑6.1	31	intergenic (+150/‑274)	metK/galP	methionine adenosyltransferase/galactose:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (12/18);  minor base A (0/1);  total (12/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	NC_000913	3,088,011	0	C	T	100.0%	106.1 / NA	31	intergenic (+151/‑273)	metK/galP	methionine adenosyltransferase/galactose:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/19);  total (12/19)

CCGCAGCATACGGTCACTTTGGTCGTGAACATTTCCCGTGGGAAAAAACCGACAAAGCGCAGCTGCTGCGCGATGCTGCCGGTCTGAAGTAATCTTTCTTCACCTGCGTTCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAACCGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTT  >  NC_000913/3087769‑3088247                                                                                                                                                                                                                                                  ||                                                                                                                                                                                                                                             cctcagtatatggTCACTGTGGTCTTGAACATTTCCCGTGGTAAAAAACCGACAAAGCGCAGCTGCTGAGTGTTGCTGTCGGTCTGAAGTAATCTTTCTTCCCCTGCTTACAAAGGCCAGCCTCGCGCTGGTCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGAAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGAGCACGTTTCACCGCAGATTATCATCACAACTTAAATTGATTacac                                                                                                                                                                                                                                      <  1:336293/240‑1 (MQ=255)  cGCAGCATACGGTCACTTTGGTCGTGACCATTTCCCGTGGGAAAAAACCGACAAAGCGCAGCTGCTGCGCGATGCTGCCGGTCTGAAGTAATCTTTCTTCACCTGCGTTCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACacc                                                                                                                                                                                                                                     <  2:420377/251‑1 (MQ=255)             gTCACTTTGGTCGTGAACATTTCCCGTGGGAAAAAACCGACAAAGCGCAGCTGCTGCGCGATGCTGCCGATCTGAAGTAATCTTTCTTCACCTGCGTTCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAg                                                                                                                                                                                                                          <  1:53994/251‑1 (MQ=255)                           gAACATTTCCCGGGGGAAAAAACCGACAAAGCGCAGCTGCTGCGCGATGCTGCCGGTCTGAAGGAATCTTTCTTCACCTGCGTGCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTa                                                                                                                                                                                                             <  1:213385/250‑1 (MQ=255)                                             aaaaCCGACAAAGCGCAGCTGCTGCGCGATGCTGCCGGTCTGAAGTAATCTTTCTTCACCTGCGTTCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATattattatt                                                                                                                                                                                          >  1:100720/1‑251 (MQ=255)                                                  cGACAAAGCGCAGCTGCTGCGCGATGCTGCCGGTCTGAAGTAATCTTTCTTCACCTGCGTTCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGtt                                                                                                                                                                                     >  2:319025/1‑251 (MQ=255)                                                          cgcAGCTGCTGCGCGATGCTGCCGGTCTGAAGTAATCTTTCTTCACCTGCGTTCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAgt                                                                                                                                                                             >  1:101706/1‑251 (MQ=255)                                                                                        aGTAATCTTTCTTCACCTGCGTTAAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAAt                                                                                                                                               <  1:80742/251‑1 (MQ=255)                                                                                                    tccccTGCGTTCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATaa                                                                                                                                      <  1:148637/245‑1 (MQ=255)                                                                                                     ccccTGCGTTCAAAGGCCAGCCGCGCGCTTGCCTTTTTCTTTTGGATATGGGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGGAAAGTCTTAATTGCAGATAACAg                                                                                                                                   <  2:218765/248‑1 (MQ=255)                                                                                                      aCCTGCGTTCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCg                                                                                                                                 >  1:260934/1‑251 (MQ=255)                                                                                                                    gCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTatgatg                                                                                                                   >  2:17859/1‑251 (MQ=255)                                                                                                                     ccacccTCGCGCTTGCATTTTCCTTTTGGATAGGCTTTCACACCGCATCCTGCAAAAAAAGCGCCCGCACAATATCATCATTCTTCCTGATCACGTTTAACCGTAGATTATCATCTCAACTGAAATTGATTACACCAACCACAACAGACAGAGATTTGTAATATTTTCATATTATTATTCGGTTTTGACAGGTGTTACATTTCTTTTCAGTAAAGTCTTAATTTCAGATACCAGCGTTTAATCTAtgatga                                                                                                                  <  2:281068/247‑1 (MQ=255)                                                                                                                             gcccTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTc                                                                                                          <  2:238288/248‑1 (MQ=255)                                                                                                                                       ttttCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTAtttt                                                                                                 <  2:260934/250‑1 (MQ=255)                                                                                                                                         ttCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCa                                                                                               <  1:389208/250‑1 (MQ=255)                                                                                                                                         ttCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCa                                                                                               <  1:84731/250‑1 (MQ=255)                                                                                                                                             tttGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCa                                                                                           <  1:346564/250‑1 (MQ=255)                                                                                                                                                                         cAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAgt                                                              <  1:237755/251‑1 (MQ=255)                                                                                                                                                                              aaaCCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAg                                                         <  2:157238/251‑1 (MQ=255)                                                                                                                                                                                 ccgcccgcACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTACAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAAc                                                                              >  1:176767/1‑227 (MQ=255)                                                                                                                                                                                 ccgcccgcACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATGATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATGTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGAGGATATAACTCAATTATTTTCATGCACTTAAATCATAAc                                                                              <  2:176767/227‑1 (MQ=255)                                                                                                                                                                                           aaTAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGCGATTCCACTGAt                                            <  2:250977/251‑1 (MQ=255)                                                                                                                                                                                                              aTCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAATAGACAAAGCTTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGCGATTACACTGATGAGATTTGCTTCCCATCtt                         >  2:421210/1‑251 (MQ=255)                                                                                                                                                                                                                   gTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTAc                    >  1:155692/1‑251 (MQ=255)                                                                                                                                                                                                                            cAGATTGTCATCAGAACTTAAAATGAATACACCAACCACGACGGATGAAGATGTGTACTATTTACATGATAATATACGGTTTTCACAGTTGTTTCATTTCTTTTTATTAAATTCTTAATTACAGATAACAGCGTTTAATCTATGATGATATAACTAAATTAGTATCATGCACATAAATCATAACTAAGATAATGGTTAGTGTAAGCGATTACGCTGATGTGTTTTGCTTCACGTCtggtttcttcgtactc           <  2:294129/251‑16 (MQ=255)                                                                                                                                                                                                                            cAGATTATCATCCCAGCTGAAATTGATTCCACAAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGATTTCACAGTTGATACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTATTCTATGATGATATACCTCAATTATTTTCATGAACTTAAATCATAAATAAGATAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTACCCATATTTTTACGTCGTACTc           >  2:231948/1‑251 (MQ=255)                                                                                                                                                                                                                            cAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTc           >  1:148159/1‑251 (MQ=255)                                                                                                                                                                                                                               aTTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCAcc        >  1:244511/1‑251 (MQ=255)                                                                                                                                                                                                                               aTTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGCGATTAAACTGATGTGATTTGCTTCACATCTTTTTATGTCGTACTCAcc        >  2:5335/1‑251 (MQ=255)                                                                                                                                                                                                                                     atcaCAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCtt  <  1:81029/251‑1 (MQ=255)                                                                                                                                                                                                                                                  ||                                                                                                                                                                                                                                             CCGCAGCATACGGTCACTTTGGTCGTGAACATTTCCCGTGGGAAAAAACCGACAAAGCGCAGCTGCTGCGCGATGCTGCCGGTCTGAAGTAATCTTTCTTCACCTGCGTTCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAACCGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTT  >  NC_000913/3087769‑3088247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑