Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,161,248 G→T G61V (GGC→GTC)  fabR → DNA‑binding transcriptional repressor FabR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,161,2480GT97.8% 134.8 / NA 46G61V (GGC→GTC) fabRDNA‑binding transcriptional repressor FabR
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (29/16);  total (30/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCCAAAGAATTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCA‑GCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCC‑GCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTCCGCTGCGTTTCGTGCCGCC  >  NC_000913/4160999‑4161492
                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                    
ggCCAAAGAATTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACCATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCA‑GCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCC‑GCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGc                                                                                                                                                                                                                                                     >  2:302238/1‑251 (MQ=255)
   aaaaGAATTGCAGAAAATATTTTTTATTGGTTTACCTTTAATTCACAATACGGGAGCAATCCAGTAGGTTCATTCTCTGGTATAGTGCCA‑GCAGTCCTTTTGGCAAGGATCCAGACATCGTGATGGGCGTAAGAGCGCAAAAAAAAGAAAAAACCC‑GCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCAtt                                                                                                                                                                                                                                                  <  1:36783/250‑1 (MQ=255)
          ttGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGCGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCA‑GCAGTACTTTTGGCAAGGATTCAGACATCGTGCTGGGCGTAAGAGCGCAACAAAAAGAAAAAACCC‑GCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGCACGCAGCTTCGCCAGACTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTccc                                                                                                                                                                                                                                            >  1:168699/1‑250 (MQ=255)
            gCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCA‑GCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCC‑GCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCAcc                                                                                                                                                                                                                                         >  1:346498/1‑251 (MQ=255)
                 aaaTATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCA‑GCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCC‑GCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGCAGCGGTCATTGCTCCCACCTCttt                                                                                                                                                                                                                                    >  1:135624/1‑251 (MQ=255)
                         tttATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCA‑GCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCC‑GCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGc                                                                                                                                                                                                                            >  2:82471/1‑251 (MQ=255)
                             ttGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCA‑GCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCC‑GCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCAttt                                                                                                                                                                                                                        >  2:331603/1‑251 (MQ=255)
                              tGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCA‑GCAGTACTTTTGGCAAGGATTCAGACATCGTGATGCGCGTAAGAGCGCAACAAAAAGAAAAAACCC‑GCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGACGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTc                                                                                                                                                                                                                       >  2:160773/1‑251 (MQ=255)
                                cGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCA‑GCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCC‑GCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCcg                                                                                                                                                                                                                     >  1:264896/1‑251 (MQ=255)
                                     ccggtcattcaCAATACGGGATAAATCCAGGATTTTCATTGTGTGGTA‑GGGGCCAAGCAGTTTTTTGGGCAAGGATGCAGACAGGGTGGGGGGGGTAAGGGCGCAAAAAAAAAAAAAACCCC‑GCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACg                                                                                                                                                                                                                <  2:135624/247‑1 (MQ=255)
                                         tcattcaCAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCA‑GCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCC‑GCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGCGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGcgtcgt                                                                                                                                                                                                               >  1:109105/1‑248 (MQ=255)
                                          gtttcaAAATACTGGAGAAATCCGGTATGTTAATTCTCTGGTATAGCGCCA‑GAATTACTTTTGGCAAGGATTGAGACATCGTGAGGGGCGTAAGAGCGCAAAAAAAAGAAAAAACCC‑GCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCACCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTAGCGGCATTTCCGCGACGTAGa                                                                                                                                                                                                            <  2:109973/248‑1 (MQ=255)
                                               acaATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCA‑GCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCC‑GCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCCTTTCCGCGACGTAGACGacct                                                                                                                                                                                                      >  2:293158/1‑248 (MQ=255)
                                                   tACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCA‑GCAGTACTTCTGGCAAGGATTCAGCCATCGTGATGGGCGTCAGAGCGCAACAAAAAGAAAAAACCC‑GCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGCGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCACACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTgggg                                                                                                                                                                                                  >  1:408505/1‑250 (MQ=255)
                                                       ggAGCAATCCTGTATGTGCATTCGCTGGTATAGAGACA‑GCAGTCCTTTTGGCAAGGATTCAGACATCGTGATGGGCGAAAGAGCGCAACAAAAAGAAAAACCCC‑GCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCGCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTg                                                                                                                                                                                               <  1:272255/250‑1 (MQ=255)
                                                           cAATCCAGTATGTTCATTCTCTGGTATAGTGCCA‑GCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAACAGAAAAAACCC‑GCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTAAAGCGGTCATTGCTCCCACCTCTTTTTATCGGGATTTCCGCGACGTAGACGAACTGGGTCTGACCAt                                                                                                                                                                                          >  1:218321/1‑251 (MQ=255)
                                                                cAGTATGTTCATTCTCTGGTATAGTGCCA‑GCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCC‑GCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCCTTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTtg                                                                                                                                                                                     >  2:61297/1‑251 (MQ=255)
                                                                              tctGGTATAGTGCCA‑GCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCC‑GCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGttt                                                                                                                                                                        >  1:86527/1‑250 (MQ=255)
                                                                                      aGTGCCA‑GCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCC‑GCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGCCGAACTGGGTCTGACCATGGTTGCTGAGAGCGGATTAATGCTACg                                                                                                                                                               >  2:352866/1‑251 (MQ=255)
                                                                                                                   cAGACATCGTGATGGGCGTAAGAGCGCAACAAAACGAAAAAACCC‑GCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCCTTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGc                                                                                                                                                                             >  2:88452/1‑209 (MQ=255)
                                                                                                                   cAGACATCGTGATGGGCGAAAGAGCGCAACAAAAAGAAAAAACCC‑GCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGt                                                                                                                                   <  1:357293/251‑1 (MQ=255)
                                                                                                                       cATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCC‑GCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCCGGCGCGTCAGCGTATCg                                                                                                                               >  2:322574/1‑251 (MQ=255)
                                                                                                                               tgagCGTAAGAGCTCAACAAAACGAAAAAACCC‑GCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGACCGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGc                                                                                                                       >  2:274192/4‑251 (MQ=255)
                                                                                                                                            cgaaaaaaaaGAAAAAACCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATc                                                                                                           <  2:325450/246‑1 (MQ=255)
                                                                                                                                                             ccc‑GCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCCTTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGACCTGGGACTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCcac                                                                                          >  1:258415/1‑250 (MQ=255)
                                                                                                                                                                             tggAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCACATTTATGGAGTTCATc                                                                          >  1:96778/1‑251 (MQ=255)
                                                                                                                                                                             tggAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCAACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATAGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCCCATTTATGGAGTTCATc                                                                          >  1:234605/1‑251 (MQ=255)
                                                                                                                                                                                   ccGCATTTAGCCACTTAAGTGCTGAACGCAGCTTCGCCAGCCTGCGTTTGCGTGACGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTCTCGGCATTTCCGCGACGTAGACGAACTGGGTCTGCCCATGGTTGATGAGAGCGGTTTACTGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGCGTGTGATCCGCCCCTCGGTCTCCACCTTTATGGAGTTCATCGGtaat                                                                    >  2:34897/1‑251 (MQ=255)
                                                                                                                                                                                    cGCATTTAGCCAATTACGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGGGATCCGCACCTCGGTCTCCACCTTTATGGAGTTCATCGGtaat                                                                    >  1:386210/1‑250 (MQ=255)
                                                                                                                                                                                          tAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGACTCCCCATTTATGGAGTTCATCGGTAATAATCCt                                                              >  1:172532/1‑250 (MQ=255)
                                                                                                                                                                                               aaTTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGcc                                                        >  1:217665/1‑251 (MQ=255)
                                                                                                                                                                                                 ttAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGAGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGCACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGATACGCCAACTCCTGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGACGGGTGATCCGCAACTCGGTCTCCACATTTATGGGATTCATCGGTACTAACCCTAACGCCtt                                                      >  2:402857/1‑251 (MQ=255)
                                                                                                                                                                                                          gAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGttatt                                             <  1:82471/251‑1 (MQ=255)
                                                                                                                                                                                                           aaCGCAGCTTCGCCAGCCTGAGTTTGCGGGAAGTGGCGCGGGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCCCCTCTGTCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTACGGttatta                                            <  2:341560/251‑1 (MQ=255)
                                                                                                                                                                                                            aCGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTtattat                                           <  1:313382/251‑1 (MQ=255)
                                                                                                                                                                                                                 gCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCgg                                      <  1:331603/251‑1 (MQ=255)
                                                                                                                                                                                                                      gCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCGTCCGGTTATTATTGCGGGAACg                                 <  1:160773/251‑1 (MQ=255)
                                                                                                                                                                                                                       ccAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGGGTGATCCGCACCTCGGTCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTACGGGAACGc                                >  2:106571/1‑251 (MQ=255)
                                                                                                                                                                                                                            ctgagttaacgggaactGGCGAGTGAAGCGGTCATTGCTCCCCCATCTTTTTATCGGCATTTCCGCGACGTCGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGACAACTCATGCGCCCGGCGCGTCAGCGTATCGCCCAAGGCGGGAGTGTGAACCGCACCTCGGTCTACCCATTTCTCGAGTTCCTCGGGAATAATCCTAACGCCTACCGGGTATTATTGCGGGTACGCTCCgg                           >  2:269371/17‑251 (MQ=255)
                                                                                                                                                                                                                                    gCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTcc                    <  2:86527/250‑1 (MQ=255)
                                                                                                                                                                                                                                       tGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACg                                 <  1:232310/234‑1 (MQ=255)
                                                                                                                                                                                                                                       tGAAGTGGCGCGTGAAGAGGTAAATGCTCCCACATCTTTTTATCGGCATTTCCGCGACGTAAAAGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGGGATCCGCACCTCGGTCTCCACATTTATGGCGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACgggatcggaagagcggcg                >  2:232310/1‑234 (MQ=255)
                                                                                                                                                                                                                                          aGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAAATGGGTCTGACCCTGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGGGATCCGCCCCTCGGGCTCCCCCTTTATGGTGTTCATCGGTAATAATCATAACGCCTTCCGGTTATTATTGTGGGAACGCTCCGGCACCCCCGCTGCgg             >  2:421221/1‑250 (MQ=255)
                                                                                                                                                                                                                                                 cgttaaGCGGTCAGTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGGTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGTCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGTTTATTATTTCGGGAACGCTCCGGCGCCTCCGCTGCTTTTCGTgc      <  2:231560/247‑1 (MQ=255)
                                                                                                                                                                                                                                                  gTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCGTCCGGTTATTATGGCGGGAACGCTCCGGCACCTCCGCTGCGTTTCGTgcc     <  2:58235/251‑1 (MQ=255)
                                                                                                                                                                                                                                                     aaGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCACATTTATGGAGTGCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTCCGCTGCGTTTCGTgccgcc  <  1:387743/251‑1 (MQ=255)
                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                    
GGCCAAAGAATTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCA‑GCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCC‑GCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTCCGCTGCGTTTCGTGCCGCC  >  NC_000913/4160999‑4161492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑