Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,993,531	T→C	R793R (CGT→CGC)	cyaA →	adenylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,993,531	0	T	C	100.0%	165.2 / NA	48	R793R (CGT→CGC)	cyaA	adenylate cyclase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/26);  total (22/26)

CAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCG  >  NC_000913/3993291‑3993776                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      cAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGGGGGGGGCGGGTTTGCCAGCGGAGGGGTCATCCCGTTTTTTTTTGTAGAAACGCAAGACGGGAATGGGTTTAAAACCTTAATTTTCGACGAAAGCAAACGGGTTGAGGGAAATTACCACTGCGAAGGACGCAAAAAGGAGGTGGTTCGGGTAGGCAGTCGGTTCTACTCGCCTACGCATGAACGCTTTACCTACg                                                                                                                                                                                                                                             >  2:125601/1‑251 (MQ=255)     cTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGAGGGGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTTGAAGCAACGCAAGAAGAGAATGGGTTTAATATCTACATTCTCGACGAAAGCAACCGGGGTGAGGGATATCAACACCGCGAAGGCAGCAAAGAGGAGCTGGTACGGGAAGGCAGGCGCTTCTACTCGGCATTGCAAGAACGCTTTTCCTACGGCTc                                                                                                                                                                                                                                         >  1:275957/1‑251 (MQ=255)           ggCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCtt                                                                                                                                                                                                                                   <  2:305891/251‑1 (MQ=255)                gTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTtcat                                                                                                                                                                                                                                <  1:113019/249‑1 (MQ=255)                       cAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGGCGGCTTTGCCAGCGAAGGGGTCATCCCATTTTTTTTCGCAGAAACGCAAGACGAGAATGGGTTTAATATCTACATTCTCCACGAAAGCCAACGGGGTGGGGGATATCACCACTGCGAAGGCAGCAAAAAGGGGCTGGTACGTGGCGTCAGGCGCTTCTACTCGTCATCGCCTGACCGCTTTTCCTACCGCTCCAGCTTCATTAACTTcc                                                                                                                                                                                                                        >  2:106117/1‑249 (MQ=255)                         ggTTGAAACCAATCACGTCAAATTCCCGGCGGTGGTGGCCGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAAAGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGGCAGTAGCTTCTACTCGTCATCGCATGACCGCTTTACCGACGGCTCAAGCTTCATCAACTTCAAcc                                                                                                                                                                                                                     <  2:78175/251‑1 (MQ=255)                                 ccAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAg                                                                                                                                                                                                             <  2:104515/251‑1 (MQ=255)                                      cACGTCAAATTACCGGCGGTGGTGGACGGCTTGGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCTCTTGTCCTACGGTTCAAGCTTCATCAACTGCAACCTGCCGCAGTTCTa                                                                                                                                                                                                        <  1:164055/251‑1 (MQ=255)                                         gTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCaa                                                                                                                                                                                                                       <  1:399334/233‑1 (MQ=255)                                                      cggTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGtt                                                                                                                                                                                        <  1:250149/251‑1 (MQ=255)                                                          ggtggACGGCTTTGCCAGCGAAGGGAGCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATAGCACCACTGCGAAGGCAGCAAAGAGGAGCTGTTACGGGACGTCAGTCGCTTCTACTCTTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACGTCATCCTGCCGCAGTTCTATCAGATTGTGAAGGTTGAt                                                                                                                                                                                     <  2:395979/250‑1 (MQ=255)                                                                                  cctcatcCGGTTCTTGTTCATACAAACGCTCGACGCGAATGGCTTTCATATCTACCTTCTCGACGCAAGCGACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCCAAGAGGAGCTAGTACGTGACGTCAGTCTCTTCTCCTCGTCTTCGCATGACCGCTTAACCTACGTCTCCAGCTTCATCAACTTAAACCTGCCGCAGTTCTATCCGTTTGTGATGGTTGATGGTcttcaaccgtacattccgttcc                                                                                                                                                            >  2:196058/3‑230 (MQ=255)                                                                                    tcatcCAGTTCTTTTTCGAAGAAACGCAAGACGAGCATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCCCTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCACCTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGCTGGTCGTGAACAGGTGATTCCGTTCCGc                                                                                                                                                          >  1:163571/1‑251 (MQ=255)                                                                                         cAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCCGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTCCGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGATGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACaa                                                                                                                                                      >  1:11365/1‑250 (MQ=255)                                                                                                ttttCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGCCGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATc                                                                                                                                              >  1:51426/1‑251 (MQ=255)                                                                                                 tttCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCg                                                                                                                                             >  1:62051/1‑251 (MQ=255)                                                                                                     gaagaaACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTa                                                                                                                                          >  1:280422/1‑250 (MQ=255)                                                                                                     gaagaaACGCAAGACGAGAATGGCTTTAATATCTACATCCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACGCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTaa                                                                                                                                         <  2:144528/251‑1 (MQ=255)                                                                                                          aaCGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATgc                                                                                                                                    >  2:282963/1‑251 (MQ=255)                                                                                                          aaCGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATgc                                                                                                                                    >  1:183546/1‑251 (MQ=255)                                                                                                            cGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATgccg                                                                                                                                  <  2:239574/251‑1 (MQ=255)                                                                                                              cAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATgccgcc                                                                                                                                <  1:359411/251‑1 (MQ=255)                                                                                                                 gACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGc                                                                                                                             >  1:424191/1‑251 (MQ=255)                                                                                                                     agaATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTTAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTGACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAAt                                                                                                                         <  2:409539/251‑1 (MQ=255)                                                                                                                     agaATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAAt                                                                                                                         <  1:217736/251‑1 (MQ=255)                                                                                                                       aaTGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGGTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCGTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCa                                                                                                                       <  1:434529/251‑1 (MQ=255)                                                                                                                               tAATATCTTCATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCAc                                                                                                                <  2:139153/250‑1 (MQ=255)                                                                                                                                         aTTCTCGACGAAAGCAACCGTGTTGAGGTATATCCCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCAGCGCATGACCGCTTTACCTACGGCGCAAGCGTCATCAACGTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGAGTCCGGTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCt                                                                                                     <  2:188486/251‑1 (MQ=255)                                                                                                                                           tctcGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTa                                                                                                    <  1:282963/250‑1 (MQ=255)                                                                                                                                              cgacgaAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTAc                                                                                                <  2:346435/251‑1 (MQ=255)                                                                                                                                                acgaAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATAGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAg                                                                                              <  1:188416/251‑1 (MQ=255)                                                                                                                                                  gaAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGc                                                                                             >  1:167192/1‑250 (MQ=255)                                                                                                                                                         aCCGGGTTGATGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTCCCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATAtttt                                                                                     <  2:242542/251‑1 (MQ=255)                                                                                                                                                                  aGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGtgatga                                                                            <  1:310250/251‑1 (MQ=255)                                                                                                                                                                          caccacTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCg                                                                    >  2:211905/1‑251 (MQ=255)                                                                                                                                                                            ccacTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGCCGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCgg                                                                   >  1:125213/1‑250 (MQ=255)                                                                                                                                                                                   gAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCg                                                             >  1:94383/1‑249 (MQ=255)                                                                                                                                                                                      ggCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAACCAGGATCACGATACTCCGCTCTTACAGCAATATTTTTCGTGATGAACGAGCCGGAAAGCGAGGCt                                                        >  1:249238/1‑251 (MQ=255)                                                                                                                                                                                         agcaAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTAATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTAt                                                     >  1:343448/1‑251 (MQ=255)                                                                                                                                                                                             aaGATGAGCTGGGACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCCGTTCTATCAGATTGTGAAGGTTGATGGTAGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGACCGATCCGGAAAGCGAGGCTTATCCgg                                                 >  2:447345/1‑251 (MQ=255)                                                                                                                                                                                                    gCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTGCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCTTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCaa                                          <  2:318922/251‑1 (MQ=255)                                                                                                                                                                                                       ggTACGTGACGTCAGTCGCTGCTACTCGTCATCGCATGACCGCTTTACCTACGTCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATc                                        <  1:159319/250‑1 (MQ=255)                                                                                                                                                                                                                  ccAGTCGCTGCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCATCCTGCCGCAGGTCTATCAGAGTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCTCGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCTGAAAGCGAGGTTTATCCGGCATGCAATCTTAGCGGAAact                            <  2:429901/250‑1 (MQ=255)                                                                                                                                                                                                                     gTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAactgac                         <  2:62051/251‑1 (MQ=255)                                                                                                                                                                                                                        gCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTAATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAACTCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTTCAGCACTATTTTTCGTGATGAACGTGCCGGCAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGACACTGACTGt                      >  1:414215/1‑251 (MQ=255)                                                                                                                                                                                                                                    cATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCctg          >  1:213730/1‑251 (MQ=255)                                                                                                                                                                                                                                        gCATGCCCGCTTTACCTACGGCACAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATGGTGAAGGTTGATGGTCGGGAACAGGGGATTCCGTTCCGCAAAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCCCGATACGCCGCAATTACAGCAATATTTGTCGTGAGGAACGTGCCGGAAAGCGAGGCGTATCCGGCATGCAATCGTAGCGGAAACTGACTGTTTCACCCGCCtgctgc      <  2:410805/251‑1 (MQ=255)                                                                                                                                                                                                                                            gACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTcg  >  2:61967/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      CAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCG  >  NC_000913/3993291‑3993776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑