| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,682,515 | C→T | M311I (ATG→ATA) | dctA ← | C4 dicarboxylate/orotate:H(+) symporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,682,515 | 0 | C | T | 96.7% | 99.0 / ‑3.1 | 30 | M311I (ATG→ATA) | dctA | C4 dicarboxylate/orotate:H(+) symporter |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (16/13); total (16/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.87e-01 | |||||||||||
GCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACT‑ACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGG‑CCTGGGCGATAAACACCGCCGCCATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGATTGAACCCAATACCA > NC_000913/3682272‑3682741 | ccctttACCGCCCAGGTTAGTCAGCGCCCGTGTTTCTAACAAAAGGCGGTCGATACCGGGGATCAGCCCCACCCCCTCTCCCGGCAAAGGCCCCCCCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCCCT‑CCCCGTTACCCCTGCCGCCCTTTTAAAAGAAAGCAGAAACACGATTAACAGCGTGTTTTGGTGGACGATATCCAGCTGACTGTTAGTGG‑CTTGGGCGAGAAACACCGCCGCTATTGTCa < 1:170129/247‑1 (MQ=255) ttACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACT‑ACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGG‑CCTGGGCGATAACCACCGCCGCTATTGTCAGGt > 1:236490/1‑250 (MQ=255) gaccagactAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGCCAAATGGCCCACCGCAGAGAGCGTCGCAGCCCGCACGCTAAAGCCACT‑ACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGG‑CCTGGGCGATAAACACCGCCGCTCTTGACAGGTATATCg > 2:45172/9‑251 (MQ=255) gACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACT‑ACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGG‑CCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCg > 1:412530/1‑251 (MQ=255) gACCAGGTTAGACAGAGCACGAGCTTCTGACCTACAGCGGTCGATACCGAGGATCAGAGCCAGCCCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACT‑ACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGCTATCCATCTGACTGTTAGTGG‑CCTGGGCGATAAACACCGCCGCTATTGTCAGGTCTATCg > 2:291349/1‑251 (MQ=255) ccccAGGTTAGTCAGCGCACGAGCTTCTGACAAAAAGCGGTCGACACCGAGGAGCGCCGCCAGCCCCGCTACCGGCAAAGGCCCCCCCGCAGAGGGCGTCGCCGCCAGCACGATAAGGCCACT‑ACCCGTTCCCCCTGCCGCCCCTTAAGAAGAAAGCAGCAACACGATTAACGGCGGTATTGGGTGGACGATATCCATCTGACTTTTAGTGG‑CCTGGGCGAAAAACACCGCCGCTATTGTCAGGTATATCg < 1:419342/249‑1 (MQ=255) gTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGCTAAAGCCACT‑ACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGCTATCCATCTGACTGTTAGTGG‑CCTGGGCGATAAACACCGCCGCTATTGTCAGGTATCTCGAttgtc > 2:103183/1‑247 (MQ=255) tAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAACCACT‑ACCCGTTACCCCTGCCGCCCCTTTAGACGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGG‑CCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGcc > 1:400601/1‑250 (MQ=255) aTAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACT‑ACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGG‑CCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGt > 1:178986/1‑251 (MQ=255) cGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACT‑ACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGG‑CCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGa > 1:89259/1‑250 (MQ=255) acagccccagccccgttccgggCAAATGGCCCACCGCAGAGAGCGCCCCCGCACGCACAAAAAAGCCACT‑CCCCGTTCCCCCTGCCCCCCCTTTAGAAAAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCAATCTGACTGTGAGGGG‑CGTGGGCGAAAAGCACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCaccacc < 2:178986/231‑1 (MQ=255) aaTGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGAAAAAGCCACT‑ACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGGGATTTGGTGGACGATATCCATCTGACTGTTAGTGG‑CCTGGGGGTTAAACACCGCCGCTATTGTCAGGAATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTcc > 1:181764/1‑251 (MQ=255) aTGGCACACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCAACT‑ACCCGTTACCCCTGACGCCCCTTTCGAAGAAAGCAGCAACACGATTCACAGCGTGATTTGGTGGCCGATATCCATCTGACTGTTAGTGG‑CCTGGGCGATAAACACCGCCGCTATTGTATGGAATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACAAGCCCCACCACCGATTTACGCCAGCCGAGTTTCTcc > 2:179974/1‑250 (MQ=255) ggCCCCCCGCGGAGGGCGCCGCCCCCAGCACAATAAA‑CCCCTCACCCGTTCCCCCGGCCCCCCCTTTAAAAAAAAGCACCAACACGATTAACAGCTTGATTTGGTGGCCGATATCCATCTGACTTTTAGGGG‑CCTGGGCGATAACCACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATc < 1:74108/251‑1 (MQ=255) gCCCCCCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACT‑ACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGG‑CCTGGGCGATAAACACCGCCGCCATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCAtat < 1:406269/251‑3 (MQ=255) ccccaccaagaaaaaGCCACT‑CCCCTTTACCCCTGCCGCCCCTTAAGAAAAAAGCAGCAACACGATTAACAGGGTGATTGGGTGGACGATACCCTTCTGCTTGTTAGTGG‑CCGGGGCAAAAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAgcg < 2:242003/239‑1 (MQ=255) gCACGATAAAGCCACT‑ACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGG‑CCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGAc > 1:422855/1‑251 (MQ=255) cACGATAAAGCCACT‑ACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGG‑CCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGAcc > 2:359932/1‑250 (MQ=255) aCGATAAAGCCACT‑ACCAGTTACCCCTGCCGCCCCTTTCGAAGAACGCATCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGG‑CCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGCCCAGCCCCACCACCGATTTCCGGCAGCCGAGTTTCTCCATCTTGt > 2:212776/1‑226 (MQ=255) ccGTTCCCCCTGCCGCCCCTTTAGAAAAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGG‑CCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTcccc < 1:149742/251‑1 (MQ=255) ccTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGG‑CCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAAt > 1:227105/1‑251 (MQ=255) ccttaaaaaaaaaGCAGCAACACGATTAACAGCGTGTTTGGGGGGACGATATCCATCTGCCTTTTAGTGG‑CCGGGGCAAAAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGttc < 2:140642/241‑1 (MQ=255) aaaaaaggcaacaccacgataaaacgCGTGATTTGGTGGACGATCTCCATCTGACTGTTAGTGG‑CCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACg < 1:125919/226‑1 (MQ=255) aaCACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGG‑CCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGAt > 1:94099/1‑251 (MQ=255) acacGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGG‑CCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGAt > 2:75671/1‑250 (MQ=255) cAGCGTGATTGGGTGGACTATCTCCATTTG‑CTTTTTGTGGGCTTGGGCTATAAACCCCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGa < 1:64829/251‑1 (MQ=255) ggtaatgtgggggACCATACCCATCGGACTGTTGGGGG‑CGTGGGAGATAAACCCCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTGCACGGATGTAGCGGATAAATTTGAAGATAc < 2:412530/240‑1 (MQ=255) gggtggACGATATCCATTGGACTGTGAGTGG‑CCGGGGCGATAACCACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAAc < 1:264907/249‑1 (MQ=255) gtgccggggCGAAAACCACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAATGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGATTGAACCCAATAcc < 1:284804/245‑1 (MQ=255) ggccggcgcaaTAACCACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGTAAGATGAAGTCCCCAGTACAATCAGCAGGTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGATTGAACCCAATACCa < 1:291349/241‑1 (MQ=255) | GCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACT‑ACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGG‑CCTGGGCGATAAACACCGCCGCCATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGATTGAACCCAATACCA > NC_000913/3682272‑3682741 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |