Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,388,041	T→G	T50P (ACG→CCG)	aaeB ←	aromatic carboxylic acid efflux pump subunit AaeB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,388,041	0	T	G	100.0%	135.6 / NA	43	T50P (ACG→CCG)	aaeB	aromatic carboxylic acid efflux pump subunit AaeB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (20/23);  total (20/23)

CAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGTACCGGCGGCAACAATCGCCGCTGTCAGTACC‑GCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAG‑CCAACAAACA‑GCGCCAGTACAATGGCGGTCGCCAG‑TTTTACCGCAAAGCG‑AATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAG  >  NC_000913/3387802‑3388285                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                          cAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGcac                                                                                                                                                                                                                                                >  2:6106/1‑250 (MQ=255)          ataACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATcgcc                                                                                                                                                                                                                                        <  2:331248/250‑1 (MQ=255)                        cccACGCATACGAGTTTTCTATTCGAACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAAGGGTGCGCGGATCATCGCAAGTATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCAGTCAGTACC‑Gcc                                                                                                                                                                                                                        <  2:436067/251‑1 (MQ=255)                              cATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACC‑GCCCAGCGt                                                                                                                                                                                                                  <  1:232511/251‑1 (MQ=255)                                            aTTCGGACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACC‑GCCCAGCGTGGCGTTTCCAGc                                                                                                                                                                                                      <  2:434720/249‑1 (MQ=255)                                                      gCGAGGATATCCAGGTACAAAACCCGGCCCAGATACCGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCCCCAGTCCGGCACTACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGCTAGCGAATCGCGCCAGAATCCGGTTCCCCTCCCGCAGCAACGGCCGGACCGGCGGCAACAATCACAGCGTTCAGTACC‑GCCCAGCGTGGCGTTTCCCGCTGGacaggcc                                                                                                                                                                                            >  2:383929/1‑243 (MQ=255)                                                        gAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGGGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATCCGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCACCGCTGTCAGTACC‑GCCCAGCGTGGCGTTTCCAACTGGCAGTGAAAG‑cc                                                                                                                                                                                       >  1:390497/1‑251 (MQ=255)                                                                       cAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACC‑GCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAG‑CCAACAAACA‑GCGCCAg                                                                                                                                                                       <  1:4022/251‑1 (MQ=255)                                                                         aaaaCCTGCCCAGATACATCACACCAGAATCATCAATAATGGTGCGCGGATCATCGAAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACC‑GCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAG‑CCAACAATCA‑GCGCCAGTa                                                                                                                                                                     <  2:390497/251‑1 (MQ=255)                                                                          aaaCCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACC‑GCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAG‑CCAACAAACA‑GCGCCAGTAc                                                                                                                                                                    <  1:378426/251‑1 (MQ=255)                                                                                 cccAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACC‑GCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAG‑CCAACAAACC‑GCGCCAGTACAATGGCg                                                                                                                                                             >  2:308759/1‑251 (MQ=255)                                                                                  ccAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACC‑GCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAG‑CCAACAAACA‑GCGCCAGTACAATGGCgg                                                                                                                                                            <  1:360066/251‑1 (MQ=255)                                                                                     gATACAGCACACCAGAATCATCAATAATGGTGCGCCGATCATCGCCATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACC‑GCCCAGCGTGGCGTTTCCAGCTGGAAGTGACAG‑CGAACAA‑CACGCGCCAGAACAATGGCgggcg                                                                                                                                                         >  2:37591/1‑248 (MQ=255)                                                                                         cAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACC‑GCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAG‑CCAACAAACA‑GCGCCAGTACAATGGCGGTCGcc                                                                                                                                                       >  2:225657/1‑249 (MQ=255)                                                                                            cacaCCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACC‑GCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAG‑CCAACAAACA‑GCGCCAGTACAATGGCGGTCGGCAG‑ttt                                                                                                                                                 >  1:264554/1‑251 (MQ=255)                                                                                               cccAGAAGCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACC‑GCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAG‑CCAACAAACA‑GCGCCAGTACAATGGCGGTCGCCAG‑TTTTAc                                                                                                                                              <  1:237930/250‑1 (MQ=255)                                                                                                      tcatcaATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGCTGCGCAAAAAGACACGCTCGAGAATAGCGCCAGAATACGGTTCAACTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCTCTGTCAGTACC‑GCCCAGCGCGGCGTTTCCAGCTGGAAGTGAAAG‑CCAACAAACA‑GCGCCAGTACAATGGCGGTCGCCCG‑TTTTACCGcca                                                                                                                                         >  1:189629/1‑247 (MQ=255)                                                                                                                 ggTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGTAACAATCGCCGCTGTCAGTACC‑GCCCAGCTTGGCGTTTCCAGCTGGAAGTGAAAG‑CCAACAATCA‑GCGCCAGTACAATGGCGGTCGCCAG‑TTTTACCGCAAATCG‑AATATGTTg                                                                                                                           <  1:55770/251‑1 (MQ=255)                                                                                                                 ggTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACC‑GCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAG‑CCCACAAACA‑GCGCAAGTACAATGGCGGTAGCAAGGTTTTAC‑GACAAGCG‑AATATgatg                                                                                                                           >  1:36305/1‑248 (MQ=255)                                                                                                                      gcgGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAACAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACC‑GCCCAGCGTGGCGTTTCCAGCTGGAAGAGAAAG‑CCAACAAACA‑GCGCCAGTACAATGGCGGCCGCCAG‑TTTTTCCGCAAAGCggctattttgggttc                                                                                                                       >  2:67557/1‑237 (MQ=255)                                                                                                                                gCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACC‑GCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAG‑CCAACAAACA‑GCGCCAGTGCACTGGCGGTCGCCAG‑GTTTACCGCAACGCG‑AATATGTTGGTTAGCAAttggga                                                                                                             >  1:272416/1‑245 (MQ=255)                                                                                                                                   atgaggATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACC‑GCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAG‑CCAACAAACA‑GCGCCAGTACAATGGCGGTCGCCAG‑TTTTACCGCAAAGCG‑AATATGTTGGTTAGCAATGGAGAAAAt                                                                                                         <  2:13025/251‑1 (MQ=255)                                                                                                                                     gatgatCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACC‑GCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAG‑CCAACAAACA‑GCGCCAGTACAATGGc                                                                                                                                                              >  2:90835/1‑198 (MQ=255)                                                                                                                                     gatgatCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACC‑GCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAG‑CCAACAAACA‑GCGCCAGTACAATGGc                                                                                                                                                              <  1:90835/198‑1 (MQ=255)                                                                                                                                         atCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACC‑GCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAG‑CCAACAAACA‑GCGCCAGTACAATGGCGGTCGCCAG‑TTTTACCGCAAAGCG‑AATATGTTGGTTAGCAATGGAGAAAATTCCCAt                                                                                                   <  1:405700/251‑1 (MQ=255)                                                                                                                                               aGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACC‑GCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAG‑CCAACAAACA‑GCGCCAGTACAATGGCGGTCGCCAG‑GTTTACCGCAAAGCG‑AATATGTTGGTTAGACATGGAGAAAATAACCAACGTGAt                                                                                             >  1:305758/1‑251 (MQ=255)                                                                                                                                                  ccGGCAAGACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACC‑GCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAG‑CCAACAAACA‑GCGCCAGTACAATGGCGGTCGCCAG‑TTTTACCGCAAAGCG‑AATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTaa                                                                                          <  1:91012/251‑1 (MQ=255)                                                                                                                                                  caggCAATACATCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAAGAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACC‑GCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAG‑CCAACAAACA‑GCGCCAGTACAATGGCGGTCGCCAG‑TTTTACCGCAAAGCG‑AATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTa                                                                                           <  1:305128/248‑1 (MQ=255)                                                                                                                                                            aGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACC‑GCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAG‑CCAACAAACA‑GCGCCAGTACAATGGCGGGCGCCAG‑TTTTTCCGCAAAACG‑AATATGTTGGGTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCAc                                                                                >  1:382157/1‑251 (MQ=255)                                                                                                                                                            aGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACC‑GCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAG‑CCAACAAACA‑GCGCCAGTACAATGGCGGGCGCCAG‑TTTTAACGCAAAGCG‑AATATGTTGGTTAGCCATGGAGAAAAAACCCATCGTGATTAACCAAACTCAc                                                                                >  1:248912/1‑251 (MQ=255)                                                                                                                                                                          ccGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACC‑GCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAG‑CCAACAAACA‑GCGCCAGTACAATGGCGGTCGCCAG‑TTTTACCGCAAAGCG‑AATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGcc                                                                  >  1:431808/1‑251 (MQ=255)                                                                                                                                                                               gatgCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACC‑GCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAG‑CCAACAAACA‑GCGCCAGTACAATGGCGGTCGCCAG‑TTTTACCGCAAAGCG‑AATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCAttt                                                              <  1:6106/250‑1 (MQ=255)                                                                                                                                                                                  gcgcAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACC‑GCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAG‑CCAACAAACA‑GCGCCAGTACAATGGCGGTCGCCAG‑TTTTACCGCAAAGCG‑AATATGTTGGTTAGCAATGGAGAAAATTCCCATCGTGATTAACCAAACTCACGCAGGCGATGGTCCATTTTACg                                                          <  2:425125/251‑1 (MQ=255)                                                                                                                                                                                      aaaaaGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACC‑GCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAG‑CCAACAAACA‑GCGCCAGTACAATGGCGGTCGCCAG‑TTTTACCGCAAAGCG‑AATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGga                                                        >  1:330795/1‑249 (MQ=255)                                                                                                                                                                                         aaGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACC‑GCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAG‑CCAACAAACA‑GCGCCAGTACAATGGCGGTCGCCAG‑TTTTACCGCAAAGCG‑AATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCCCGCAGGCGATGGGCCCTTTTACGGAAGAAc                                                   >  1:160985/1‑251 (MQ=255)                                                                                                                                                                                          aGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACC‑GCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAG‑CCAACAAACA‑GCGCCAGTACAATGGCGGTCGCCAG‑TTTTACCGCAAAGCG‑AATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACg                                                  <  2:257803/251‑1 (MQ=255)                                                                                                                                                                                                gATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACC‑GCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAG‑CCAACAAACA‑GCGCCAGTACAATGGCGGTCGCCAG‑TTTTACCGCAAAGCG‑AATATGTTGGTTAGCAATGGAGAAAATGCCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCt                                            <  1:163532/251‑1 (MQ=255)                                                                                                                                                                                                 aTAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACC‑GCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAG‑CCAACAAACA‑GCGCCAGTACAATGGCGGTCGCCAG‑TTTTACCGCAAAGCG‑AATATGTTGGTTAGCAAGGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTg                                           <  2:264554/251‑1 (MQ=255)                                                                                                                                                                                                           gcgcCAGAATACGGTTCACCTCCCGCAGCAATGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACC‑GCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAG‑CCAACAAACA‑GCGCCAGTACAATGGCGGTCGCCAG‑TTTTACCGCAAAGCG‑AATATGTTGGGTAGCAATGTATAAAATGCCCATCGTGATGAACCATACTCACGCAGGCGATGGGCCATTTTACGTAAGAACGAATCCTGGCGTgcgtcg                                 <  2:25644/251‑1 (MQ=255)                                                                                                                                                                                                                      cgttcatcctcccccgcaaaaGGCCGGCCCGGCGGCAACAATCGCCG‑TGTTAATACCCGCCCAGCTTGG‑GTTTTCCGCCGGGAGTGAAAGGCCAACAAACA‑GCGCAAGTCCAATGGGGTTGGCAAG‑TTTTACCGCAA‑GCGGAATATTTGGGTTAGCAAGGGAAAAAATCCCCTTCGTGATAAACCAAACCCCCGCGGCCGATGG‑CCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTg                     <  1:92744/233‑1 (MQ=255)                                                                                                                                                                                                                                     agcaAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACC‑GCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAG‑CCAACAAACA‑GCCCCAGTACAATGGCGGTCCCCAG‑TTTTACCCCAAAGCG‑AATATGTTAGTTCACAATGGCGAAAAAACCCATCGGGATTAACCAAACTCCCCCCGGCGATGGGACATTTTACGGAAGAACGAAACCCGGCTTTAGTCGCGGACTTGTTTTCCAGTCaccacccc       >  1:204813/1‑249 (MQ=255)                                                                                                                                                                                                                                        aaaGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACC‑GCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAG‑CCAACAAACA‑GCGCCAGTACAATGGCGGTCGCCAG‑TTTTACCGCAAAGCG‑AATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCCTTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCCCTGt    >  1:91712/1‑251 (MQ=255)                                                                                                                                                                                                                                          aGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACC‑GCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAG‑CCAACAAACA‑GCGCCATTACAATGGCGGTCGCCAG‑TTTTACCGCAAAGCG‑AATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAg  <  1:435657/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                          CAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGTACCGGCGGCAACAATCGCCGCTGTCAGTACC‑GCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAG‑CCAACAAACA‑GCGCCAGTACAATGGCGGTCGCCAG‑TTTTACCGCAAAGCG‑AATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAG  >  NC_000913/3387802‑3388285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑