Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,926,292	C→T	intergenic (+96/‑16)	queF → / → ppnN	7‑cyano‑7‑deazaguanine reductase/nucleotide 5'‑monophosphate nucleosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,926,292	0	C	T	100.0%	149.4 / NA	45	intergenic (+96/‑16)	queF/ppnN	7‑cyano‑7‑deazaguanine reductase/nucleotide 5'‑monophosphate nucleosidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/19);  total (26/19)

GGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACT  >  NC_000913/2926046‑2926534                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   gaaaCGCATCGTTAAGGCCCTGTTTCGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTTGCGCAGTAATATCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATGCGTCTCAt                                                                                                                                                                                                                                                 <  2:235810/248‑1 (MQ=255)         tCTTTAATGACCTGTTAAGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGttt                                                                                                                                                                                                                                        >  1:395829/1‑251 (MQ=255)            ttAATGACCTGTTACGCTTCTGCCAGCCAGACAAATTGAGCGTTTACGCACGTTATACCCGCCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTTCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGGCATCAGGCAGGGCTATTGTAATCGAAGGGAATGACGATATTCGTCTCATAAGGGGTTTTTc                                                                                                                                                                                                                                     >  2:442584/1‑251 (MQ=255)            ttAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGtt                                                                                                                                                                                                                                         <  1:375996/247‑1 (MQ=255)            ttAATGACCTGTTACGCTTCTGCCAGCCAGAAAAAGTGAGCGTGTACGCACGTTATTCCCGTCGTGGCGGTCTGGACATTAAGCCGTGGCGCAGTAATAGCGATTATGTCCCATCGACCACAAGTCTGGTTCGGCAATAAATTGTTTCTCAATTTGGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGtttt                                                                                                                                                                                                                                       <  2:419929/249‑1 (MQ=255)                 gACCTATTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTCGACATTAACCCGTGGCGCAGGAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGCCGATATTCGTATCATAAGGAGTTTTTCTTGAt                                                                                                                                                                                                                                >  2:366523/1‑251 (MQ=255)                           gCTTCTGCCCGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAAACCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGCAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTAAACATAtt                                                                                                                                                                                                                      >  2:372555/1‑251 (MQ=255)                                   cagccagAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCt                                                                                                                                                                                                              >  1:243663/1‑251 (MQ=255)                                    agccagAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCtt                                                                                                                                                                                                             <  2:13185/251‑1 (MQ=255)                                     gccagAAAAATTGAGCGTTTACGCACGTTATTCCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGGAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTg                                                                                                                                                                                                            <  2:286241/251‑1 (MQ=255)                                        agAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCt                                                                                                                                                                                                         >  1:382220/1‑251 (MQ=255)                                            aaaTTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCAt                                                                                                                                                                                                     >  1:3423/1‑251 (MQ=255)                                            aaaTTGAGCGTTTACGCACGATATACCCGTCGTGGCGGACTGGACATTAAACCGAGGCGCAGTAATAGAGATTTTGTACCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTTAATTTTGCGTGCTGGGTTCACGAAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCAt                                                                                                                                                                                                     >  2:417472/1‑251 (MQ=255)                                               ttGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATgg                                                                                                                                                                                                   >  1:346915/1‑250 (MQ=255)                                                    cGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTTCCATCGCCCACAAGACTGGTTCGGAAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGGCATCCGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTATACATATTAGCCCGCTTGGCTCCACGGATAtgt                                                                                                                                                                                             >  2:77507/1‑251 (MQ=255)                                                                ttATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTgg                                                                                                                                                                                 >  1:33875/1‑251 (MQ=255)                                                                  ataCCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGa                                                                                                                                                                                >  2:320494/1‑250 (MQ=255)                                                                    aCCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCCGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGt                                                                                                                                                                             >  2:99496/1‑251 (MQ=255)                                                                    aCCCGGCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGt                                                                                                                                                                             <  2:297055/251‑1 (MQ=255)                                                                          cgtGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATGTGTTCTCAATTGTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATAGTCGTCTCATAAGTAGTTTTTCTTGATTACACATATTAGCCCGCTGGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGatat                                                                                                                                                                       <  2:189575/251‑1 (MQ=255)                                                                             ggCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCAGCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTGTTTCTTGATTACACATATTAGCCCGCTTGGCGCCATGGATATGGTGTCGCAGCTAGAAGTGGAGATGCt                                                                                                                                                                    <  2:63398/251‑1 (MQ=255)                                                                                     ggACATTAACCCGTGGCGCAGTCATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGc                                                                                                                                                             >  1:393043/1‑250 (MQ=255)                                                                                              cccGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCcagc                                                                                                                                                    >  2:138344/1‑250 (MQ=255)                                                                                                       cAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTcc                                                                                                                                                                                                       <  2:37682/190‑1 (MQ=255)                                                                                                       cAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTcc                                                                                                                                                                                                       >  1:37682/1‑190 (MQ=255)                                                                                                           aaTAGCGATTTTGTCCCATCGTCCACAAGACTGTTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGTTCCATGGATATGTTGTCGCAGCGGTAAGTGGATATGCTTAAACGCACCGCCAGCAGCGGCCTCTATCa                                                                                                                                      <  1:149919/251‑1 (MQ=255)                                                                                                           aaTAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGCAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATc                                                                                                                                       >  1:205805/1‑250 (MQ=255)                                                                                                                              cGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCa                                                                                                                   <  1:290495/251‑1 (MQ=255)                                                                                                                                         cTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGCAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACt                                                                                                        >  2:74885/1‑251 (MQ=255)                                                                                                                                                          ttttgCTCAATTTTGCGTGCTGGATTCACGCAGAATGGTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCAGAAGGAGTTTTGCTTGATTACCCATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAATTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACGGTTCACTTGCCGTACTGAACTCCGGTAGTTTg                                                                                        <  1:237986/250‑1 (MQ=255)                                                                                                                                                           ttttCTCAATTTTGCGTTCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGa                                                                                       <  1:194568/250‑1 (MQ=255)                                                                                                                                                            tttCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGAcc                                                                                     >  1:78143/1‑251 (MQ=255)                                                                                                                                                             ttCTCAATTTTGCGTGCTGGATTCCCGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCTTCTCATAAGGAGTTTTTCTTGATTACACATATGAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGTAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGAcc                                                                                     <  1:417472/250‑1 (MQ=255)                                                                                                                                                             tgctcAATTTTGCGTGCTGGATTCACGCAGAAGTTTGTGAAAGTTCATCAGGCAGGGCTATTGTAATAAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGAcc                                                                                     <  1:241395/248‑1 (MQ=255)                                                                                                                                                                         cGTGCTGTATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCaa                                                                          >  2:18821/1‑249 (MQ=255)                                                                                                                                                                          gTGCTGGATTCACGCAGAAGGTTATGTAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATAGTCGCCTAATAAGGCGTTTTACTTGATTACACATATTAGCCCCCTTGGCTACATGGCTATGTTGTCGCAGCTGGAAGCGGATATGCTTAAACGCCCCGCACGTATCGACATCTATCAAATGTTTCGCAACTGTTAACTTGCCGTACTGAACTACGGTCGTTTGTACGATAACAGcca                                                                          >  2:146546/1‑246 (MQ=255)                                                                                                                                                                                           aaGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTg                                                      <  1:66252/251‑1 (MQ=255)                                                                                                                                                                                                        gTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACAAATATTAGCCCGCTTGGCTCCAGGGATATGTTGTCGCAGTTGGAAGTGGATATGCTAAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGatat                                         <  1:176000/251‑1 (MQ=255)                                                                                                                                                                                                                  aggGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTg                               >  2:226529/1‑251 (MQ=255)                                                                                                                                                                                                                   gggCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTgc                              >  1:80165/1‑251 (MQ=255)                                                                                                                                                                                                                                cAAAGGGAATGCCGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCAGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACgcgg                 >  2:191789/1‑251 (MQ=255)                                                                                                                                                                                                                                     ggAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTa             >  1:201956/1‑250 (MQ=255)                                                                                                                                                                                                                                       aaTGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTaa            >  2:290632/1‑249 (MQ=255)                                                                                                                                                                                                                                       aaTGACGATATTCGTCTCAAAAGGAGTTTTTCTTAATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCGCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAg          <  2:35377/251‑1 (MQ=255)                                                                                                                                                                                                                                                atTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCAGGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACt  <  1:99496/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   GGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACT  >  NC_000913/2926046‑2926534

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑