Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,410,419	G→A	L312L (CTG→TTG)	yfbS ←	putative transporter YfbS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,410,419	0	G	A	97.7%	134.0 / NA	45	L312L (CTG→TTG)	yfbS	putative transporter YfbS
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base A (24/19);  minor base C (1/0);  total (25/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

AGCATACCGATCAGTTTCCAGTTACCCACAACCAGGATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAGTACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCA‑GAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGC  >  NC_000913/2410178‑2410665                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                        aGCATACCGATCAGTTTCCAGTTACCCACAACCAGGATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCgg                                                                                                                                                                                                                                                >  1:107212/1‑251 (MQ=255) aGCATACCGATCAGTTTCCAGTTACCCACAACCAGGATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCgg                                                                                                                                                                                                                                                >  1:103743/1‑251 (MQ=255)      cccGACCAGTTTCCAGTTTCCCACAACCAGGATGATATCGCCCAGCAGCAGAGCCGCAGCCGCCAGCGAACCTTCCAGCGCCATGGCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATTCGGTCTGTCTCATGAACTAATGAAATCTCTGCCTTGCGCGCGTCAAGGTTCTGATCATAATAATTCTCTCCGCGCAATACCATCTGCTCCa                                                                                                                                                                                                                                           <  2:401935/250‑1 (MQ=255)          aTCAGTTTCCAGTTACCCACAACCAGGATGATATCGCCCAGCAGCAGATGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCa                                                                                                                                                                                                                                        >  2:95039/1‑250 (MQ=255)                        cccaacaAACAGGATGAATTCGGCTAGCCGCAGAGGCTCATCCGCAAGCGAATCTACCAGCGCCACTCCAGTTCGCTTTAGCCCCACAACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACGCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAGTGCCATCGGCTCCAGCAATTGCTCACTACaaa                                                                                                                                                                                                                         <  2:250564/247‑1 (MQ=255)                                 cAGGATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGACATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCAGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCgg                                                                                                                                                                                                                 >  2:128738/1‑250 (MQ=255)                                   ggATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCgg                                                                                                                                                                                                                 >  1:210032/1‑248 (MQ=255)                                     atgatATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCgg                                                                                                                                                                                                                 >  2:301736/1‑246 (MQ=255)                                               ccAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCTCCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGGCGGAGATCGACATCAGcc                                                                                                                                                                                                  <  1:130190/251‑1 (MQ=255)                                                cagcagcagAGGCTCATCCGCCAGCGAACCTGCCAGCGCCACGCCATTGCGCTTTAGCCCCTCCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCGCTGCCAGGCCCTCATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATGGCTCACTACAAAATGGCCTTAGAGCGACATCAGCCg                                                                                                                                                                                                 <  2:210032/251‑1 (MQ=255)                                                                     cAGCGAACCTCCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCaaaa                                                                                                                                                                            <  1:38795/251‑1 (MQ=255)                                                                            ccTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCAcg                                                                                                                                                                     <  1:308762/251‑1 (MQ=255)                                                                                  aGCGCCACGCCATTGCGCTTTAGCCCCACCTCATTCAGTCCGTAGCTGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACCCGCCGCGCAATACCATCGGCTCCAGCAATTGCGCTCTACAAAAGTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAAAGTCAcgcgcgcg                                                                                                                                                               <  2:230427/251‑1 (MQ=255)                                                                                       cacgccaTTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAAtt                                                                                                                                                          <  1:182920/251‑1 (MQ=255)                                                                                       cacgccaTTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCAGCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAAtt                                                                                                                                                          <  2:187180/251‑1 (MQ=255)                                                                                          accaTTGAGATTTAGACCCACCACATTCAGCCCGTAGCGGGTACGAACACGAATTTCGCGCACCGATTTACCACTAACTTCTCACTCAGGAATTCATGATATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCCTCGGCTCCAGCAATTGCTCACTACAAAATTGACGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCAAGCGCGCGAAATTc                                                                                                                                                         >  2:61759/2‑249 (MQ=255)                                                                                           ccaTTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTc                                                                                                                                                         >  1:53634/1‑248 (MQ=255)                                                                                           ccaTTGCGCTTTAGCCCCACCACATTCAGTCCGGAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAACTTGACGGAGATCGACATCAACCGCAGACATATCAATAAGCACAACGTAACGCGCTCGAAATTc                                                                                                                                                         >  1:383991/1‑248 (MQ=255)                                                                                                      tAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCA‑GAAACCCCATTa                                                                                                                                           >  2:389805/1‑250 (MQ=255)                                                                                                                      aGTCCGTAGCGGGTACGAAAACCAATTGCGCGCACAGATTTACCAATCAGTTCTGACTCAGGAAGTAATGAAATCTCTGCCATGCCCACATCAAGGGACTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCA‑GAAACCCCATTAACGTTCACGATAAcgcg                                                                                                                          <  2:19878/251‑1 (MQ=255)                                                                                                                         ccGTAGCGGGTACGAGAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATAGCAATAAGCAAAACGTCACGCGCGCGAAATTCA‑GAAACCCCATTAACGTTCACGATAACGcgac                                                                                                                        <  2:366374/250‑1 (MQ=255)                                                                                                                                gggTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCA‑GAAACCCCATTAACGTTCACGATAACGCGACGAAAAcgc                                                                                                                <  2:351120/251‑1 (MQ=255)                                                                                                                                 agTACGACAACCATTTTCGCGCACAGATTTACCAATCAGTTCTGACTCAGGCATTAATGAACTCTCTCCCATGCCCACATCAAGGGCCTGGACAGAAAAATACTCGCCGCGCACTCCCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGACATTCA‑GAAACCCCATTAACGTTCACGATAACGCGACGAAAAcgcc                                                                                                               >  2:22590/2‑251 (MQ=255)                                                                                                                                      gAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCA‑GAAACCCCATTAACGTTCACGATAACGCGACGAAAACgccgcc                                                                                                            >  1:422804/1‑249 (MQ=255)                                                                                                                                                 tCGCGCACCGATTTAACAATCAGATCTGACTCAGGAATTAACGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTAGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGAACGCCATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCA‑GAAACACCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCaccacc                                                                                               >  2:265686/1‑251 (MQ=255)                                                                                                                                                        ccGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCA‑GAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACg                                                                                        <  2:75540/251‑1 (MQ=255)                                                                                                                                                          gATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCA‑GAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGtt                                                                                      >  2:141589/1‑251 (MQ=255)                                                                                                                                                          gATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGACCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGATCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCA‑GAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGtt                                                                                      >  1:202329/1‑251 (MQ=255)                                                                                                                                                                      aGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCA‑GAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGCCGTTAGCGCCATAACg                                                                          >  2:250409/1‑251 (MQ=255)                                                                                                                                                                          cTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCA‑GAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTc                                                                       >  1:311673/1‑250 (MQ=255)                                                                                                                                                                                   gAATTAATGAAATCTCGGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACACGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAAGAAGCAAAACGTCACGCGCGCGAAATTCA‑TAAACCCCATTAACGTTCACGAGAACGCGACAAAAACGCCGCCAGCGTTCAACGTCGATGACGTTAGCGCAATAACGCTCACGTAAtttt                                                             <  2:52496/251‑2 (MQ=255)                                                                                                                                                                                     aTTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCA‑GAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTgag                                                           <  1:412570/251‑1 (MQ=255)                                                                                                                                                                                     aTTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCA‑GAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTgag                                                           <  1:128738/251‑1 (MQ=255)                                                                                                                                                                                     aTTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTAGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAAAGTCACGCGCGCGAAATTCA‑GAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGACAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCCCGTAATTTga                                                            >  1:277680/1‑250 (MQ=255)                                                                                                                                                                                       tAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCA‑GAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTg                                                             >  1:199047/1‑247 (MQ=255)                                                                                                                                                                                             aaccgcgaccATACCCACATCCAGCGCCAAGTCATAAAAACACTCGCCGCGCAAAACCATCGCCACCAGCAATTGCACACTACAAAAACGCCGGAGAGCGACAACAGCCGAAGACATACCAATAAGCAAAACGTCCCGCGCGC‑AAACTCAAGAAACTCAAATAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGGACAACACCGATGACCTTCGCGCCATAACGCGCACGTAATTTCAGATCATCCa                                                   <  2:107212/243‑1 (MQ=255)                                                                                                                                                                                              aTCTCTGCCATGCACACATCAAGGGCCTGGAAAGAAAAATACTAGCCGCGCAATAACATCGGATCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCA‑GAAACCCCATTAACGTTCACGATAACGCGACGAAAACGACGCCAGCGTTCAACACCGATGAAGTTAGCGCCATAACGCTCACGTAATTTGAGATCATcc                                                    >  1:126023/1‑249 (MQ=255)                                                                                                                                                                                               tctcTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCA‑GAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTCATTTGAGATCATcccgc                                                 >  1:105515/1‑248 (MQ=255)                                                                                                                                                                                                    gCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCA‑GAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTg                                            <  2:106349/251‑1 (MQ=255)                                                                                                                                                                                                      cATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCA‑GAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGGTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGAc                                          <  2:409860/251‑1 (MQ=255)                                                                                                                                                                                                               agcAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATGGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCA‑GAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTg                                 <  1:22590/249‑1 (MQ=255)                                                                                                                                                                                                                        cTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCA‑GAAACCCCATTAACGTTCACGATAACGCGAAGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTCATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCgg                        >  1:315772/1‑251 (MQ=255)                                                                                                                                                                                                                               gAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCA‑GAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAAt                 >  1:255454/1‑251 (MQ=255)                                                                                                                                                                                                                                aaaaaTACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAACATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGAAAAACGTCACGCGCGCGAAATTCA‑GAAAACCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAAAGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCCTTGGCGATCCGGGGCGAAt                 >  2:40713/1‑250 (MQ=255)                                                                                                                                                                                                                                              cgcAATACCATCGGCTCCAGCAATTGCACACTACCCAATTGCCGGAGCTCGACATCAGCCGCAGACATATCAATAAGCCAAACGTCACGCGCGCGAAATTCC‑GAACCCCCATTACCGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGCCGTTAGCGCCATAACGATCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCgcgc  >  2:187529/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                        AGCATACCGATCAGTTTCCAGTTACCCACAACCAGGATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAGTACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCA‑GAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGC  >  NC_000913/2410178‑2410665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑