Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,195,035	G→A	A246T (GCG→ACG)	metG →	methionine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,195,035	0	G	A	90.0%	79.0 / ‑4.6	34	A246T (GCG→ACG)	metG	methionine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (1/2);  major base A (17/10);  minor base C (0/3);  total (18/16)
Fisher's exact test for biased strand distribution p-value = 7.04e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGACTGAACTGATCGAGCCGAAATCGGTGGTTTCTGGCGCTACGCCGGTAATGCGTGATTCTGAACACTTCTTCTTTGATCTGCCCTCTTTCAGCGAAATGTTGCAGGCATGGACCCGCAGCGGTGCGTTGCAGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACGCGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGGAAGAAAGACTCCACCGCCGAGCTGTACCACTTCATCGGTAAAGATATTGTTTACTTCCACAGCCTGTTCTGGCCTGCCATGCTGGAAGGCAGCAACTTCCGCAAGCCGTCCAACCTGTTTGTTCATGGCTATGTGAC  >  NC_000913/2194802‑2195282                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                        cgactgaacgactcgctccGAAATCGGTGGTTTCTGGCGCTACGCCGTTAATGCGTGATTCTGAACACTTCTTCTTTGATCTGCCCTCTTTCAGCGAAATGTTTCAGTCATGCTCCCGCAGCGGTGCGTTGCAGTATCAGGTGGTAAATAAAATGCAGGAGTGGTTTGAGTCTGGCCTGCAACAGTTGGTTATCTCCCGCGACGCCCCTTTCTTCTGTTTTGTTATTCCGAACGCGCTGGACAAATATTTc                                                                                                                                                                                                                                        <  2:376351/234‑1 (MQ=255)           gaTCGAGCCGAAATCGGTGGTTTCTGGCGCTACGCCGGTAATGCGTGATTCTGAACACTTCTTCTTTGATCTGCCCTCTTTCAGCGAAATGTTGCAGGCATGTACCCGCAGCGGTGCGTTGCAGGAGCAGTTGGGAAATAAAATGCAGGAGTGGTTTTAATCTTGCCTGCAGCAGGGGGATACCTCCCGCGACGCCCTTTACTTCTGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTctgg                                                                                                                                                                                                                               <  1:49625/250‑1 (MQ=255)           gaTCGAGCCGAAATCGGTGGTTTCTGGCGCTACGCCGGTAATGCGTGATTCTGAACACTTCTTCTTTGATCTGCCCTCTTTCAGCGAAATGTTGCAGGCATGGACCCGCAGCGGTGCGTTGCAGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTCCGTctggc                                                                                                                                                                                                                              >  2:102657/1‑251 (MQ=255)              cGAGCCGAAATCGGTGGTTTCTGGCGCTACGCCGGTAATGCGTGATTCTGAACACTTCTTCTTTGATCTGCCCTCTTTCAGCGAAATGTTGCAGGCATGGACCCGCAGCGGTGCGTTGCAGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTctggctgg                                                                                                                                                                                                                           <  1:102657/251‑1 (MQ=255)                                    ggCGCTAAGCCGGTAATGCGTGATTCTGAACACTTCTTCTTTGATCTGCCCTCTTTCAGCGAAATGTTGCAGGCATGGACCCGCAGCGGTGCGTTGCAGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATggg                                                                                                                                                                                                     >  1:319591/1‑251 (MQ=255)                                                          aTTCTGAACACTTCTTCTTTGATCGGCCCGCTTTCAGCGAATTGTTGCAGGCATGGACCCGCAGCGGTGCGTTGCAGGAGCAGGTGGCAAATAAAATGCAGGAGGGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGAc                                                                                                                                                                               <  2:360281/251‑1 (MQ=255)                                                                       cttcttTGATCTGCCCTCTTTCAGCGAAATGTTGCAGGCATGGACCCGCAGCGGTGCGTTGCAGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGAc                                                                                                                                                                   >  1:392755/1‑250 (MQ=255)                                                                       cttcttTGATCTGCCCTCTTTCAGCGAAATGTTGCAGGCATGGACCCGCAGCGGTGCGTTGCAGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGGTCTTTCAAGAATCTGTGCGACAAGCGCGGCGAc                                                                                                                                                                   >  1:116297/1‑250 (MQ=255)                                                                         tcttTGATCTGCCCTCTTTCAGCGAAATGTTGCAGGCATGGACCCGCAGCGGTGCGTTGCAGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGAc                                                                                                                                                                   <  1:221107/248‑1 (MQ=255)                                                                         tcttGGATCTGCCCTCGTTCAGCGAAATGTTGCAGGCATGGACCCGCAGCGGTGCGTTGTAGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCTACGCCCCTTACTTCGGTTTTGAAATTCCGAACTCGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGAc                                                                                                                                                                   <  1:270889/248‑1 (MQ=255)                                                                            ttGATCTGCCCTCTTTCAGCGAAATGTTGCAGGCATGGACCCGCAGCGGTGCGTTGCAGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCg                                                                                                                                                               <  2:135089/249‑1 (MQ=255)                                                                                   gCCCTCTTTCAGCGAAATGTTGCAGGCATGGACCCGCAGCGGTGCGTTGCAGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATGCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGc                                                                                                                                                          <  1:191093/247‑1 (MQ=255)                                                                                   gCCCTCGTTCAGCGAAATGTTGCAGGCATGGACCCGCAGCGGTGCGTTGCTGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCGTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAg                                                                                                                                                           <  1:431559/246‑1 (MQ=255)                                                                                     ccTCTTTCAGCGAAATGTTGCAGGTATGGACCCGCAGCGGTGCGTTTCAGGAGCAGGTGTCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTGCTTCGGTTTTGAAATTCCGAACGCGCCGGGCAAATATTTCTTCGTCTCGCTGGAGGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGAt                                                                                                                                                    <  1:419833/251‑1 (MQ=255)                                                                                        ctTTCAGCGAAATGTTGCAGGCATGGACCCGCAGCGGTGCGTTGCAGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATg                                                                                                                                                   <  1:416251/249‑1 (MQ=255)                                                                                                 aaaTGTTGCAGGCATGGACCCGCAGCGGTGCGTTGCAGTAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCGGGCTGGACGCACCGATTGGCTACATGGGTGCTTGCAATAATCTGTGCGACAAGCGCGGCGTCAGCGTAAGCTTCGATGAAGACTGgaat                                                                                                                                        <  2:88774/251‑2 (MQ=255)                                                                                                      atGCAGGCATGGACCCGCAGCGGTGCGTTGCAGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTTAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGgaagaa                                                                                                                                      >  2:285903/2‑248 (MQ=255)                                                                                                             cATGGACCCGCAGCGGTGCGTTGCAGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGGAAGAAAGACTCCAcc                                                                                                                            >  2:338460/1‑251 (MQ=255)                                                                                                                       cggcggtgcggtGCAGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTAAATCTGGCCTGCAACAGTGGGATAACTCCCGCGACGCCCCTTACTTCGGTTCTGAAATTCCGAACCCGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCTTAAGCTTCGATGAATACTGGAAGAAAGACTCCACCGCCGAGCTGt                                                                                                                  <  1:88262/249‑1 (MQ=255)                                                                                                                               cgtggCAGGAGCAGGGGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGTATATCTCCCGGGACGCCCCTTACTACGGTTTTGAAATTCCAACCCCGCCGGGCAAATATTTCTACGTCTGGCTGGACGCCCCGATTGGTTACATGGGTTCTTCAAAGAATCTGTGCGCCAAGCCCGGCGACATCTTAACCTTCGATGAATCCTGAAAGAAAGACTCCACCGTCGAGCTGTACCACTTc                                                                                                          <  2:374386/247‑1 (MQ=255)                                                                                                                                   gCAGGAGCAGGTGGAAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGGAAGAAAGACTCCACCGCCGAGCTGTACCACTTCATCg                                                                                                      <  1:137434/251‑1 (MQ=255)                                                                                                                                         gCAGGTGGCCAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGCCGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGGAAGAAAGACTCCACCGCCGAGCTGTACCACTTCATCGGTaa                                                                                                  >  1:151236/1‑249 (MQ=255)                                                                                                                                          cAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGGAAGAAAGACTCCACCGCCGAGCTGTACCACTTCATCGGTaa                                                                                                  >  1:310344/1‑248 (MQ=255)                                                                                                                                              tGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGGAAGAAAGACTCCACCGCCGAGCTGTACCACTTCATCGGTAAAGATAtt                                                                                           >  2:167990/1‑251 (MQ=255)                                                                                                                                                                aGTGGTTTGAATCTGGCCTGCCACAGTGGGATATCTCCCCCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGATCTTTCCAGAATCTGTGCGGAACGAGCGGCGACAGCGCAAGCTTCGATGAATACTGGAAGAAAGACTCCACCGCCGAGCGCTACAACTTCATCGGTAAAGGTTTTGATTCCTTCAACAGCCTg                                                                         >  2:41413/1‑251 (MQ=255)                                                                                                                                                                   ggTTTGAATCTGGCCTGCAACAGTGGAATATCTCCCGCGACGCCCCTTAATTCGGTTTTGAAATTCCGACCACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGGCAGAAAGACTCCACCGCCGAGCTGTACCACTTCATCGGTAAAGATATTGTTTACTTCCACAGCCTGTTc                                                                      >  1:130346/1‑251 (MQ=255)                                                                                                                                                                      ttGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGGAAGAAAGACTCCACCGCCGAGCTGTACCACTTCATCGGTAAAGATATTGTTTACTTCCACAGCCTGTTATgg                                                                   >  2:298702/1‑251 (MQ=255)                                                                                                                                                                      tggAATCTGGTCTGCAACGGTGGGATATCTCCCGCGACGCCCCTTCCTTCGGTTTTGAAATTCCGACCCCGCCGGGCAATTATTTCTACGTCGGGCTGGACGCACCGATGGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGCCAGCGTAAGCTTCGATGAATACTGGAAGAAAGACTCCACCGCCGAGCTGTACCACTTCATCGGTAAAGATATTGTTTACTTCCACAGCCTGTTCTg                                                                    <  2:347440/248‑1 (MQ=255)                                                                                                                                                                                ccTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGGAAGAAAGACTCCACCGCCGAGCTGTACCACTTCATCGGTAAAGATATTGTTTACTTCCACAGCCTGTTCTGGCCTGCCATGc                                                         >  2:83168/1‑251 (MQ=255)                                                                                                                                                                                             gATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCAGGGCAAATATTTCTACGTCTGGCTGGCCGCACCGATTGGCTAAATGGGCTCTTTCAAGACTCTGTGCGCCAAGCGCGGCGACAGCGTAAGCTTCGCTGAATACTGGACGAAAGACTCCACCGCCGAGCTGTACCACTTCATCGGTAAAGATCTTGTTTACTTCCACAGCCTGTTCTGGCCTGCCATGCTGGAAGgcagca                                             >  1:425920/1‑250 (MQ=255)                                                                                                                                                                                                  ctcCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGGAAGAAAGACTCCACCGCCGAGCTGTACCACTTCATCGGTAAAGATATTGTTTACTTCCACAGCCTGTTCTGGCCTGCCATGCTGGAAGGCAGCAACTTcc                                       >  1:85907/1‑251 (MQ=255)                                                                                                                                                                                                                                 aTTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGACCGCGTAAGCTTCGATGAATACTGGAAGAAAGACTCCACCGCCGAGCTGTACCACTTCATCGGTAAAGCTATTGTTTACTTCCACAGCCTGTTCTGGCCTGCCATGCTGGAAGGCAGCAACTTCCGCAAGCCGTCCAACCTGTTTGTTCATGGCt         >  1:441440/1‑250 (MQ=255)                                                                                                                                                                                                                                      gAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGGAAGAAAGACTCCACCGCCGAGCTGTACCACTTCATCGGTAAAGATATTGTTTACTTCCACAGCCTGTTCTGGCCTGCCATGCTGGAAGGCAGCAACTTCCGCAAGCCGTCCAACCTGTTTGTTCATGGCTAtgtg    >  2:326117/1‑250 (MQ=255)                                                                                                                                                                                                                                       aaCGCGACGGGCCAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGGAAGAAAGACTCCACCGCCGAGCTGTACCACTTCATCGGTAAAGATATTGTTTACTTCCACAGCCTGTTCTGGCCTGCCATGCTGGAAGGCAGCAACTTCCGCAAGCCGTCCAACCTGTTTGTTCATGGCTATGTGAc  >  1:108310/1‑251 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                        CGACTGAACTGATCGAGCCGAAATCGGTGGTTTCTGGCGCTACGCCGGTAATGCGTGATTCTGAACACTTCTTCTTTGATCTGCCCTCTTTCAGCGAAATGTTGCAGGCATGGACCCGCAGCGGTGCGTTGCAGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACGCGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTTCTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGGAAGAAAGACTCCACCGCCGAGCTGTACCACTTCATCGGTAAAGATATTGTTTACTTCCACAGCCTGTTCTGGCCTGCCATGCTGGAAGGCAGCAACTTCCGCAAGCCGTCCAACCTGTTTGTTCATGGCTATGTGAC  >  NC_000913/2194802‑2195282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑