Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,700,743	C→T	D463D (GAC→GAT)	malX →	PTS enzyme IIBC component MalX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,700,743	0	C	T	100.0%	160.0 / NA	44	D463D (GAC→GAT)	malX	PTS enzyme IIBC component MalX
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/24);  total (20/24)

CGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTACAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGGTCTGATGCATACTGTCCAGGCATAAGGATAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTC  >  NC_000913/1700500‑1700987                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     cGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGATAATAtt                                                                                                                                                                                                                                                <  2:332249/250‑1 (MQ=255)   aCTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGATATTATTGTc                                                                                                                                                                                                                                             >  1:79889/1‑251 (MQ=255)          ggtgTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGATAATATTGTCAGCCTc                                                                                                                                                                                                                                       <  2:186520/250‑1 (MQ=255)                         gCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGATAATATTGTCAGCCTCGATAACTGCATTAccc                                                                                                                                                                                                                       <  1:183976/251‑1 (MQ=255)                         gCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGATAATATTGTCAGCCTCGATAACTGCATTAccc                                                                                                                                                                                                                       >  2:330065/1‑251 (MQ=255)                         gCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCAGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGATAATATTGTCAGCCTCGATAACTGCATTaacc                                                                                                                                                                                                                       >  1:49882/1‑248 (MQ=255)                          cATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGATAATATTGTCAGCCTCGATAACTGCATTAcacg                                                                                                                                                                                                                      >  1:239684/1‑248 (MQ=255)                                  gTCAACCAAGTGGTACATGGTGCCAGTTGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGATAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGtt                                                                                                                                                                                                              <  2:383117/251‑1 (MQ=255)                                     aaCCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGATAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGt                                                                                                                                                                                                           <  1:421726/251‑1 (MQ=255)                                                             ggtggCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGATAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTa                                                                                                                                                                                   <  1:237384/251‑1 (MQ=255)                                                                  cggcAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGATAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAAtgtg                                                                                                                                                                              <  1:296914/251‑1 (MQ=255)                                                                           ggTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGATAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTg                                                                                                                                                                     >  2:86390/1‑251 (MQ=255)                                                                               tGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGATAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGGTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAg                                                                                                                                                                  <  1:208576/250‑1 (MQ=255)                                                                                   gTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCAGAAAACCCCGGGTCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCTGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGATAATATTGGCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACaa                                                                                                                                                             <  2:316298/251‑1 (MQ=255)                                                                                                     cGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGATAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCgtagt                                                                                                                                           >  1:327608/1‑251 (MQ=255)                                                                                                     cGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGATAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCgtagt                                                                                                                                           <  2:91043/251‑1 (MQ=255)                                                                                                        ttCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATCGCGAAGGTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCTGGGTAAATCAGGTTACAACGTTCCTTCAATCCTCGAAGCATTAGGCGGTGCCGATAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGGTTGGCTGGGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGTGCAACTGGCGTAGTAc                                                                                                                                         <  2:46111/250‑1 (MQ=255)                                                                                                        ttCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGATAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTAc                                                                                                                                         >  2:125934/1‑250 (MQ=255)                                                                                                              aTCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGATAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTACAACTTa                                                                                                                                   >  1:22080/1‑250 (MQ=255)                                                                                                                    cGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGATAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTACAACTTAATCAACa                                                                                                                            <  1:330065/251‑1 (MQ=255)                                                                                                                               gAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGATAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAAGCGGGCAATTGGCGTAGTACAACTTAATCAACATAACCTGc                                                                                                                    <  2:85474/248‑1 (MQ=255)                                                                                                                                  aaCCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGATAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTACAACTTAATCAACATAACCTGCAGgttg                                                                                                              <  1:157918/251‑1 (MQ=255)                                                                                                                                         gggCGCGATAGAGAAGTTGCTAGCTCAATCGAAAAAGCCGATGCCGGTGCGCCGGATAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGATCATATTGCCAGCCTCGATAACTGCATTACACGTCTGCGTTTGTCTGTGAAAGATATGTCTCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTACAACTTAATCAACTTAACCTGCAGGTTGTTATCgg                                                                                                       >  2:260829/1‑251 (MQ=255)                                                                                                                                             gcgATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGATAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGatat                                                                                                                                                                                              >  2:168618/1‑160 (MQ=255)                                                                                                                                             gcgATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGATAATATTGGCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGatat                                                                                                                                                                                              <  1:168618/160‑1 (MQ=255)                                                                                                                                                   gCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGATAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTACAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGtt                                                                                             <  2:402617/251‑1 (MQ=255)                                                                                                                                                       aGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGATAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCAGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTACAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTtcagt                                                                                         <  1:124247/251‑1 (MQ=255)                                                                                                                                                                   aaTCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGATAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTACAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGATg                                                                             >  1:147522/1‑251 (MQ=255)                                                                                                                                                                               cGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGATAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTACAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCgttc                                                                 >  2:135449/1‑248 (MQ=255)                                                                                                                                                                                gTTGCCGGTGCGCCGGGAAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGATAATATTGTCAGACTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTACAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACCAGTTCAGTCAGTAAAAGATGAAATGGCCGGTCt                                                                >  2:35320/1‑251 (MQ=255)                                                                                                                                                                                    ccGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGATAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTACAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGGTCTGATg                                                            <  1:125123/251‑1 (MQ=255)                                                                                                                                                                                          cgcCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGATAATATTGTCAGCATCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTACAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGACAGTAAAAGATGAAATGGCCGGTCTGATGCATACt                                                      >  2:376377/1‑251 (MQ=255)                                                                                                                                                                                          ccccGGGTAAATCAGGTTACAACGTTCCTTCAATCCTCGAATCATGAGGCGGTGCCGATAATATTGTCAACCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTACAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATTGCCGGTCTGATGCATACt                                                      <  2:126557/249‑1 (MQ=255)                                                                                                                                                                                             cGGGTAAATCAGGTTACAACGTTCCTGCACTCCTCGAAGCATTAGGCGGTGCCGATAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTACAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGGTCTGATGCATACTGTc                                                   >  1:363420/1‑251 (MQ=255)                                                                                                                                                                                             cGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGATAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTACAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGGTCTGATGCATACTGTc                                                   >  2:194213/1‑251 (MQ=255)                                                                                                                                                                                               ggTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGATAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTACAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGTAACAGATGAAATGGCCGGTCTGATGCATACTGTcc                                                  >  2:131960/1‑250 (MQ=255)                                                                                                                                                                                                  aaagcAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGATAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGTCAATCGGGCAATTGGCGTAGTACAACTTAATCAACATATTCTGCATGTTGTGATCTGGCCACAAGTTCAGGCAGTAAAAGATGAAATGGCCGGTCTGAGGCATACGGTCCAgg                                               <  2:185219/246‑1 (MQ=255)                                                                                                                                                                                                           tacagctttCCTGCAATCCTCGAAGCATTGGGCGGTGCCGATAATATTTTCAGCCGCGATAACTGCATTGCCCGTCTGCGTTTGTTGGTAAAAGATATGTCGCTTGTGAATGTGCAGGCACGTAAGGACAAGCTGTCAATTGGCGTAGTACAACGTATTCAGTATATCCTGGAGGTTGTGAGCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGGTCTGATGCATACTGTCCAGGCATAAGGAt                                      <  1:47800/243‑1 (MQ=255)                                                                                                                                                                                                                        cAATCCTCGAAGCATTAGGCGGTGCCGATAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTACAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGGTCTGATGCATACTGTCCAGGCATAAGGATAAGATATGTTCGAt                        >  1:54920/1‑251 (MQ=255)                                                                                                                                                                                                                                  agaaTTAGGCGGTGCCGATAATATTGTCAGCCTCGATAACTGCATTACCCGGCTGCGTTGGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTACAACTTAATCAACATAACCTGCAGGCTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGGTCTGATGCATACTGTCCAGGCATAAGGATAAGATATGTTCGATTTTTAAAAgg              <  2:3536/248‑1 (MQ=255)                                                                                                                                                                                                                                    cATTAGGCGGTGCCGATAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTACAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGGTCTGATGCATACTGTCCAGTCATAAGGATAAGATATGTTCGATTTTTCAAAGGTc            <  1:125934/251‑1 (MQ=255)                                                                                                                                                                                                                                         ggcgtggCCGATAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGGTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTACAACTTAATCAACATAACCTGCAGGTTTTTATCGGGCCACAAGTTCAGCGAGTAAAAGATGAAATGGCCGGTCTGATGCATACTGTCCAGGCATAAGGATAAGATATGTTCGAtt                       <  2:283760/229‑1 (MQ=255)                                                                                                                                                                                                                                         ggCGGTGCCGATAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTACAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTGAGTAAAAGATGAAATGGCCGGTCTGATGCATACTGTCCAGGCATAAGGATAAGATATGTTCGAtt                       >  1:283760/1‑235 (MQ=255)                                                                                                                                                                                                                                               gCCGATAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTACAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGTTCTGATGCATACTGTCCAGGCATAAGGATAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTc  >  1:328441/1‑250 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     CGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTACAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGGTCTGATGCATACTGTCCAGGCATAAGGATAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTC  >  NC_000913/1700500‑1700987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑