| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,652,331 | T→C | intergenic (+2346/+397) | ydfD → / ← ynfP | lysis protein/protein YnfP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,652,331 | 0 | T | C | 95.7% | 67.4 / ‑5.7 | 23 | intergenic (+2346/+397) | ydfD/ynfP | lysis protein/protein YnfP |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (11/11); total (12/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
GTTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGT‑CATTCATCTTATTAAGCCAGCA‑ATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATG‑TTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCC > NC_000913/1652094‑1652571 | gtTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGT‑CCTTCATCTTATTAAACCAGCA‑ATCGACGCTCTCCGGCGTCAGAAGACATTAACGAGGCTGAGCAAAAAGCATATCCTTGATG‑TTCACCTCAGGGAGTATgg > 2:73964/1‑251 (MQ=255) gTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGT‑CATTCATCTTATTAAGCCAGCA‑ATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATG‑TTCACCTCAGAGAGTATGGcagaac < 2:307957/251‑1 (MQ=255) aggaCATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCCATCGGGT‑CATTCATCTTATTAAGCCCGCA‑ATCTACGCTCTCCGGAGACAGATGAGAATAACGAGACTGAGCAAAGAGCATATCATTGATG‑TTCACCTCAGAGAGAATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTTAACCTGAAgtgtaaacg > 1:132741/1‑246 (MQ=255) tAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGT‑CATTCATCTTATTAAGCCAGCA‑ATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATG‑TTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGt > 1:47788/1‑250 (MQ=255) aGATGTGAAAAATTGAACAATGAGGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAGCAATCGGGT‑CATGCATCTTATTAAGCCAGCA‑ATCGGCGCTCTCCGGAGTCAGATTATATTAACTAGTCTGAGCAAAGAGCATAGCATTGAGG‑TTCACCTCTGATAGTATGGCAGAACATAAAAACAAAGATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTaa < 1:404512/251‑1 (MQ=255) aCAATGATAGTCAGGAGAAATTTAGCAAAAGACCGTTTCACAGTACCAAAAACACAGGCGAGAACCCATCGGGAACAT‑CCTCTTTTTAAGCCACCACAAC‑ACGCTCTCCGGAGTCAGATTGCATTGACGCAACTAAGCAAAAAGCATATCCTTTTTGGTCCACT‑CCGAGAAAATGGCAGAACAGAAAAACCAAAATTCACCTTTGTTTTTCAACCTTTCGTGGCAGCGCGAGAAAAAAATTAtagggacc > 2:306831/1‑243 (MQ=255) tgatgaTCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAACAATCGGGT‑CATTCCTCTTATTAAGACAGCA‑ATCGACGCTCTCCGGAGTCCGATGACATTAACGAGACCGAGCAAAGAGCATATCATTGATG‑TTCACCTCAGAGAGTATGGCAGAACAGAAAAAAAAAAATGCACCTTTGTTTTTTAACCTGAAGTGTCAGCGAGAGTAAAAAATTATTGTGACCAtttt > 1:262535/1‑251 (MQ=255) aaaGACAGTTTTACGGGACCAAAAACACAGGCGGGAACCAATCGGGT‑CATTCATCTTATTAAGCCAGCA‑ATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCAGTGATG‑TTCACCTCAGAGAGTATGGAAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGAt < 2:353304/251‑1 (MQ=255) gcccGTTTCTCGGTTTCAAAAACACAGGCGTGAACCAATCGGGT‑CATTCAACTTATTAAGCCAGCA‑ATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATG‑TTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTTCCGTTGACTCAATAAGGCAGAtgtg < 1:377317/249‑1 (MQ=255) aaaaaCACAGGCGGGAACCAATCGGGT‑CATTCATCTTATTAAGCCAGCA‑ATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATG‑TTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAAc < 1:241988/251‑1 (MQ=255) aaTCGGGT‑CATTCATCTTATTAAGCCAGCA‑ATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATG‑TTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCg < 1:73964/251‑1 (MQ=255) aTCGGGT‑CATTCATCTTATTAAGCCAGCA‑ATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATG‑TTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCg > 1:85956/1‑250 (MQ=255) cagtcatCTTATTAAGCCAGCA‑ATCGACGCGCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATG‑TTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAAGTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGGTAAAACGTGCCGGACTCCGCCATCGAAAATCa < 2:259852/247‑1 (MQ=255) catCTTATTAAGCCAGCA‑ATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGATCAAATAGCAGATCATTGATG‑TTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCCGTGGTTGGCATCCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCTTTTTACCGTGGGCTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATc < 1:191242/250‑1 (MQ=255) catCTTATTAAGCCAGCA‑ATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATG‑TTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGAGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATc > 2:311836/1‑250 (MQ=255) cTTATTAAGCCAGCA‑ATCGACGCTCTCCGGAGTCAGATGACCTTCACGAGACTGAGCAAAGAGCATATCATTGATG‑TTCACCTCAGAGAGTATGGcaga > 2:257565/1‑99 (MQ=255) tattaAGCCAGCA‑ATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATG‑TTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGATTCCGCCATCGAAAATCATATCAg < 2:438876/247‑1 (MQ=255) tattaAGCAAGCA‑ATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATG‑TTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCg > 1:118026/1‑250 (MQ=255) attaAGCCAGCA‑ATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATG‑TTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCga < 1:298617/250‑1 (MQ=255) aTGACATTAACGAGACTGAGCAAAGAGCATATCAGTGATG‑TTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTgg < 1:272502/251‑1 (MQ=255) gAGCAAAGAGCATATCATTGATG‑TTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCAttt > 2:389816/1‑250 (MQ=255) cATATCATTGATG‑TTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGAAGCAGCGATACAACGTGCAGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAAcc > 1:88556/1‑250 (MQ=255) tGATG‑TTCACCTCAGAGAGTATGGCAGAACAGACAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATAATTATGCCTGCAGGGCCCTGACAGCTGGAGCTAACCCGGCATTTATAGCCAAACAGATGGGcc > 2:11616/1‑251 (MQ=255) | GTTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGT‑CATTCATCTTATTAAGCCAGCA‑ATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATG‑TTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCC > NC_000913/1652094‑1652571 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |