Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,098,620	T→A	V245V (GTT→GTA)	ghrA →	glyoxylate/hydroxypyruvate reductase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,098,620	0	T	A	100.0%	102.3 / NA	30	V245V (GTT→GTA)	ghrA	glyoxylate/hydroxypyruvate reductase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (13/17);  total (13/17)

CTGGAGTCGAACCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTTAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGCGCACGCGGCTACTAATAAAGCATCAGGATTCCTGCTATCCTTGGCGGG  >  NC_000913/1098377‑1098857                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                              cTGGAGTCGAACCCGTAAATCGTGGCCTGGCGTGAAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGCCGGGTATTGATTACTTTGTTACCGAATACCCCTGAAACCGTCGGCGTTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCCACCTGGCGCGTGGTGGTCATGTTGGGGTAGATGTCCTGCTCGCGGAGCTGGATAGCGGCAAAGTAAAAGgcg                                                                                                                                                                                                                                        >  1:184055/1‑251 (MQ=255)           acacGTAAAGCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTTGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGa                                                                                                                                                                                                                              <  1:408776/248‑1 (MQ=255)            cccGTAAATCGTGGCCTGGCGTGCAAAGCTTTTCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGAt                                                                                                                                                                                                                             <  2:93757/251‑1 (MQ=255)              cGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGTGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGt                                                                                                                                                                                                                           <  1:173417/251‑1 (MQ=255)               gTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACGCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGAGAGCGGCAAAGTAAAAGGCGCAATGTTGGATGtt                                                                                                                                                                                                                          <  1:351402/251‑1 (MQ=255)                      gTGGCCTGGCGTGCCAAGCTTTGCCGGACGGGAAGCACTGTCTGCATTTCTGAGCCAATGTAGGGGATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGGGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGGAAAAGGCGCAATGTTTGATGGTTTTAATc                                                                                                                                                                                                                   >  2:290142/1‑251 (MQ=255)                          ccTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAAGGTCGGGTATTGATTAATTTGTTACCGAATACCCCGGAAACCGTCTGCATTATTAATCAACAAGTACGCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGGGGAAGATGACCTGCTCGCGGCGCTGGAGAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGa                                                                                                                                                                                                               <  2:431902/251‑1 (MQ=255)                                   cAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTAcc                                                                                                                                                                                                      >  1:201502/1‑251 (MQ=255)                                               ggACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTcc                                                                                                                                                                                          >  2:373286/1‑251 (MQ=255)                                                     gaagagCTGTCTGCATTTCTGAGCCAATGACGGGTATTTCATAATTTGGTACCGAATACCCCTGACACCTTCGGCATTATTAATAAACAATTACTCGAAAAATTACCGGATGGCGCGGATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGCTAGCGGCAAAGTAAAAGGCGAAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTTAAAGTCCGCTCTg                                                                                                                                                                                    >  2:28530/1‑251 (MQ=255)                                                                                   cGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAAcaccac                                                                                                                                                       <  2:208924/250‑1 (MQ=255)                                                                                        aTTGATTAATTTGTTACCGAATACCCCTGACACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTcg                                                                                                                                                 >  1:343228/1‑251 (MQ=255)                                                                                                          gAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCg                                                                                                                               <  1:56975/251‑1 (MQ=255)                                                                                                                      aaCCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTgg                                                                                                                   <  2:347610/251‑1 (MQ=255)                                                                                                                         cGTCGGCATTATTACTCCACACCTAATCGCAGAATTACCGGCTGGCCCGTATCTCCTCCCCCTGGCGCGTGGTGTTCAACTTGTGGAAGATGACCCGCTCGAGGCGCTGGATAGCGGCGCAGTAACAGGCGCAACGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGATAGTCCGCTCTGGCAACATCCACGCGGGACGATAACACAACATGTCGCCGCGATTCCCCGTCCCGCTGAAGCTGTggggt                                                                                                                >  2:190586/1‑248 (MQ=255)                                                                                                                            cGGCATGATTAATCAACAAGTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCACCCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCGTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCGCATGTCGCCGCGAGTACCCGTCCCGCTGAAGCTGTGGAGTACa                                                                                                             <  2:218079/251‑1 (MQ=255)                                                                                                                                attattAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGCGTACATTtc                                                                                                         >  1:410570/1‑251 (MQ=255)                                                                                                                                          aacaaTTACCCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCGGGCGCGTGGTGTTCAGGTTGTGGAAGATGACCGGCTCGCTGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTATCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCAtt                                                                                               <  2:264959/251‑1 (MQ=255)                                                                                                                                                       aaaaTTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGaaa                                                                                  <  1:273471/251‑1 (MQ=255)                                                                                                                                                         aaTTACCGGATGGCGCGTATCTCCTCAACCGGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGaaaaa                                                                                <  2:377057/251‑1 (MQ=255)                                                                                                                                                                    ggCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAggg                                                                      <  1:90820/250‑1 (MQ=255)                                                                                                                                                                         gTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCg                                                                >  1:71646/1‑251 (MQ=255)                                                                                                                                                                                           gcgTGGGGTTCATGTTGTGGAAGATGCCCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGTAATGTTGGATGTTGTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGCGCAc                                              <  2:239945/251‑1 (MQ=255)                                                                                                                                                                                                      aTGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGCGCACGCGGctacta                                    >  1:352296/1‑250 (MQ=255)                                                                                                                                                                                                              gAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGAGCACTCGGCTACTAATAAAGCAt                           <  1:111651/251‑1 (MQ=255)                                                                                                                                                                                                                aGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGCGCACGCGGCTACTAATAAAGCATCa                         <  2:14218/251‑1 (MQ=255)                                                                                                                                                                                                                  aTGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAACAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGCGCACGCGGCTACTAATAAAGCATCAgg                       >  2:79739/1‑251 (MQ=255)                                                                                                                                                                                                                            cgcgGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTTGATTTTTTTAATCGTGAACCCATACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACCCCACATTTCGCCGCGATTACCCGTCCCGCTTAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGCGCACGCGGCTACTAATAAAGCATCAGGATTCCTGCTa             <  2:285263/251‑1 (MQ=255)                                                                                                                                                                                                                                       aTAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGCGCACGCGGCTACTAATAAAGCATCAGGATTCCTGCTATCCTTGGCggg  >  1:25190/1‑251 (MQ=255)                                                                                                                                                                                                                                        tAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGCGCACGCGGCTACTAATAAAGCATCAGGATTCCTGCTATCCTTGGCggg  >  1:105785/1‑250 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                              CTGGAGTCGAACCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTTAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGCGCACGCGGCTACTAATAAAGCATCAGGATTCCTGCTATCCTTGGCGGG  >  NC_000913/1098377‑1098857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑