Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,069,743	C→T	A33T (GCA→ACA)	rutG ←	pyrimidine:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,069,743	0	C	T	100.0%	163.1 / NA	52	A33T (GCA→ACA)	rutG	pyrimidine:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/17);  total (35/17)

GCCCAGCGCAATGCTGATATTCGGGTTAATGCCCTGACCGTTAAAGCCCGTCGCGGCGATCACCACGCCGACAAAAGCCG‑CGCTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAA‑CATCGCCACCGCGTGTTGAACGCCCATTACTGCCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACC  >  NC_000913/1069498‑1069983                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 gCCCAGCGCAATGCTGATATTCGGGTTAATGCCCTGACCGTTAAAGCCCGTCGCGGCGATCACCACGCCGACAAAAGCCG‑CGCTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAA‑CATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCt                                                                                                                                                                                                                                             >  2:125424/1‑251 (MQ=255)             gCTGATATTCGGGTTAATGCCCTGACCGTTAAAGCCCGTCGCGGCGATCACCACGCCGACAAAAGCCG‑CGCTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGCAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATAGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAA‑CATCGCCACCGCGTGTTGACCGCCCATTACTGTCGTCTGAGCAAACGGc                                                                                                                                                                                                                                  >  2:21297/1‑250 (MQ=255)             gCTGATATTCGGGTTAATGCCCTGACCGTTAAAGCCCGTCGCGGCGATCACCACGCCGACAAAAGCCG‑CGCTGACGCCGAGATAACTGGGAACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAA‑CATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAAAGGc                                                                                                                                                                                                                                  >  2:409311/1‑250 (MQ=255)                      cGGGTTAATGCCCTGACCGTTAACCCCCGCCGCGGCTATCACCACGCCGACAAAAGCCG‑CGTTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAA‑CATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCa                                                                                                                                                                                                                         <  1:25134/250‑1 (MQ=255)                         gtaaaggccctgacctgaaaaGCCCGTCGCGGCGATCACCCCGCCGACAAA‑GCCGGCGTGGGAGCCGAGAAAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAA‑CATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGATCAAACGGCAATCGTTCATcc                                                                                                                                                                                                                      <  1:307339/232‑1 (MQ=255)                           tAATGCCCTGACCGTTAAAGCCCGTCGCGGCGATCACCACGCCGACAAAAGCCG‑CGCTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAA‑CATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGgc                                                                                                                                                                                                                   >  1:335964/1‑251 (MQ=255)                                cccTGACCGTTAAAGCCCGTCGCGGCGATCACCACGCCGACAAAAGCCG‑CGCTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAA‑CATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGAc                                                                                                                                                                                                              >  2:200254/1‑251 (MQ=255)                                      ccGTTAAAGCCCGCCGAGGCGATCACCACGCCGACAAAAGCCG‑CGCTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGCATCGGCATTAACACCGTCGCGCCAAA‑CATCGCCACCGCGTGTTGAACGCCCATTAATGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCAcgcc                                                                                                                                                                                                        >  2:268254/1‑251 (MQ=255)                                         ttAAAGCCCGTCGCGGCGATCACCACGCCGACAAAAGCCG‑CGCTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAA‑CATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCAcgccgcc                                                                                                                                                                                                     >  2:311414/1‑250 (MQ=255)                                               cccGTCGCGGCGATCACCACGCCGACAAAAGCCG‑CGCTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAA‑CATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGt                                                                                                                                                                                               >  1:36903/1‑251 (MQ=255)                                                       ggcgATCACCACGCCGACAAAAGCCG‑CGCTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATCACGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAA‑CATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCg                                                                                                                                                                                       >  1:202021/1‑251 (MQ=255)                                                                   gCCGACAAAAGCCG‑CGCTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAA‑CATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGcc                                                                                                                                                                           >  1:76839/1‑251 (MQ=255)                                                                                  cgcTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGCAACACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAA‑CATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAAATGCCAGTGAGGAAAACCg                                                                                                                                                             >  2:40380/1‑251 (MQ=255)                                                                                                       ggACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAA‑CATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGtctc                                                                                                                                        >  1:272349/1‑251 (MQ=255)                                                                                                       gaaCGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGCCATTAAAATGGAACGAATGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTC‑CACCAAAACATCGCCACCGCGTGTTGAACGCCCATTACTATCGTCTGAGCAAAAGTCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTATAGTTCTAATTTACCTGCCAATAACGAAAACCGACCATTGCCCTCAGCTGtctc                                                                                                                                        >  2:350163/3‑251 (MQ=255)                                                                                                        gcgggccccgcgGGTAAAAAAGAAAAACGGTAACGTGCAGAGCCCCGAGATTAAAATGGAAAGATTGGTTACCAGCCCCATCTGAACCGGCATGAACGCCGTCGCGCCAAA‑CAGCGCCACCGCGGGTTGAACGCTCAGTACTGTCGCCTGAGTAGCCGGCAACCTTTCATCCGTCGCGACCACGCCGCTCTCTGTAGAGGACGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTcc                                                                                                                                       <  1:417945/245‑1 (MQ=255)                                                                                                            cgcccgccGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAA‑CATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCCTCAGCTGTCTCCTTa                                                                                                                                    >  1:246798/1‑250 (MQ=255)                                                                                                                 ccggaaaaaaaaaaaaaaCAGAAACGTGCCGAGCCCCGACATTAAAAGGGAAAGATTGGAATCCACCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAA‑CACCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAaggagg                                                                                                                              <  2:359752/238‑1 (MQ=255)                                                                                                                    ggTAATAAAGAAAAACAGTACCGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAA‑CATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGtt                                                                                                                            >  1:136733/1‑250 (MQ=255)                                                                                                                                   cAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAA‑CATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCAt                                                                                                            >  1:41213/1‑251 (MQ=255)                                                                                                                                   cAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAA‑CATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCAt                                                                                                            <  1:200254/251‑1 (MQ=255)                                                                                                                                    aGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAA‑CATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATc                                                                                                           >  1:141764/1‑251 (MQ=255)                                                                                                                                          gtgcagaaccccGACATAAAAATGGAAAGATTGGGATCCAGCCCCACAAGAATCGGCATCAACACCGTCGCGCCAAA‑CATCGCCCCCGCGTGTGGACCGCCCATTACGGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACAACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCATGGCGCAGCAGcgcg                                                                                                     <  2:38811/243‑1 (MQ=255)                                                                                                                                          gTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAA‑CATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGcgcg                                                                                                     >  1:256861/1‑251 (MQ=255)                                                                                                                                            gcagATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAA‑CATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTACCTGCCAGTGAGGAAAACCGAACATTGCCAGCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTg                                                                                                   >  2:338167/4‑251 (MQ=255)                                                                                                                                             ccGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAA‑CATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTg                                                                                                   >  2:266547/1‑250 (MQ=255)                                                                                                                                               gATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAA‑CATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGAt                                                                                                 >  1:307910/1‑250 (MQ=255)                                                                                                                                                        cATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAA‑CATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATc                                                                                       >  2:78904/1‑251 (MQ=255)                                                                                                                                                        cATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAA‑CATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATc                                                                                       >  1:100511/1‑251 (MQ=255)                                                                                                                                                        aaTTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAA‑CCTCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCGTCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATc                                                                                       <  1:409311/250‑1 (MQ=255)                                                                                                                                                              aaTGGAAAGATTGTGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAA‑CATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCTGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGACCATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAAcc                                                                                  >  2:62930/1‑250 (MQ=255)                                                                                                                                                              aaTGAAAAGATTGGGATGCAGCCACATCAGAATCGGCATTAACACAGACGCGCCAAA‑CATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGGGATAACTGCGATCGAAcc                                                                                  >  2:62935/1‑250 (MQ=255)                                                                                                                                                                tGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAA‑CATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACcac                                                                                >  2:44352/1‑250 (MQ=255)                                                                                                                                                                tGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAA‑CATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGGCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACcac                                                                                >  2:341419/1‑250 (MQ=255)                                                                                                                                                                           ggATCCAGCCCCATCAGAATCGGCATTAACCCCGTCGCCCCAAA‑CACCCCCACCGCGTGTTGACCGCCCATCCCTGTCGTCGGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAg                                                                    <  2:438376/251‑1 (MQ=255)                                                                                                                                                                                 aGCCCCCTCAGAATCGGCATTAACACCGTCGCGCCAAA‑CATCGCCACCGCGTGTTGAACGACAATTACTGTCGTCTGAGCAAACGGCAATCGTTAATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGCGAGGCAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTCCGATCGAACCACACCAGACCGTAGggagtg                                                              >  2:280211/1‑251 (MQ=255)                                                                                                                                                                                  gCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAA‑CATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCAc                                                                                                                                                                                                           >  1:166040/1‑109 (MQ=255)                                                                                                                                                                                  gCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAA‑CATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCAc                                                                                                                                                                                                           <  2:166040/109‑1 (MQ=255)                                                                                                                                                                                     ccATCAGAATCGGCATTAACACCGTCGCGCCAAA‑CATCGCCACCGCGTGTTGAACGCCCCTTACTGTCGTCTGAGCAACCGGCCATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGAGCGCATCAGCGCGTGATAACCGCGATCGAGCCCCACCAGCCCGTGGggggtggtgt                                                          >  2:57578/1‑251 (MQ=255)                                                                                                                                                                                           gAATCGGCATTAACACCGTCGCGCCAAA‑CATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGACCCACACCAGCCCGTAGGGTGTGGGGTGACGAt                                                    >  2:329127/1‑251 (MQ=255)                                                                                                                                                                                              ccGGCATTAACACCGTCGCGCCAAC‑CATCGCCCCCGGGTGTTGAACGCCAATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGaa                                                 <  2:405869/250‑1 (MQ=255)                                                                                                                                                                                                ggCATTAACACCGTCGCGCCAAA‑CATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCCTCCGGCGCGACCACGCCGCTCTCTGTAGAGGACGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACAACACCAGCACGGAGGGTGTGGTGTGACGATGAATc                                               >  2:128117/1‑251 (MQ=255)                                                                                                                                                                                                ggCATAAACACCGTCGAGCCAAA‑CCTCGCCAAACCGTGTTGAACGCCCATTACTGTCGTCTGAGCAACCGGAAATAGTTCCTCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTACCTGCCAGTGCGGAAAACCGAACATTACCATCAGCTGTCTCCTTCAGGAGGCTAACAAGCCGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGCGTGGTGACACGATGactc                                               >  2:26368/1‑248 (MQ=255)                                                                                                                                                                                                     tacacccctCGCGCCAAA‑CACCCCCACCGCGTGTTGAACTCCCTTGACTGTCGTCTGAGCAAACGGCACTCGTTCATCCGGGGCGACCACGCCGCTCGCTGTAGAGGTCGATTTTAACGGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGGTAACAAGCATGGTGCATCAGCGCGTGATAACTGCGATCGAAGCGCTCCAGCTCGTAGTGTGTGGTGTGACGATGAAGCGCtt                                           <  2:312113/242‑1 (MQ=255)                                                                                                                                                                                                      aaCACCGGCGCGCCAAA‑CACCGCCACCGCGTGTTGAACGCCCATTCCTGTCGTCTGGGAAAACGGCAATCTTTCATCCGGCGCGCCCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACGGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTc                                          <  1:191979/250‑1 (MQ=255)                                                                                                                                                                                                              cgcgCCAAA‑CATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGggtgtggt                                                            >  1:271680/1‑224 (MQ=255)                                                                                                                                                                                                                cgcCAAA‑CATCGCCGCCGCGGGTTGAACTCCCATTACTTTCTTCTGAGCAAACGGCAATCGTTCATCCGTCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGATCATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTTTGGTGTTACGATGAATCTCTTCg                                         <  2:268055/241‑1 (MQ=255)                                                                                                                                                                                                                cgcCAAA‑CATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAACCGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTACTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCg                                         >  1:268055/1‑241 (MQ=255)                                                                                                                                                                                                                   cAAA‑CATCGCCCCCGCGTGTTGAACTCCCATTACTGTCGTCTGAGCAAACGCCTATCGTCCTGCCGGCGGGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACa                            <  2:257302/251‑1 (MQ=255)                                                                                                                                                                                                                        cATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAAt                        <  1:74809/251‑1 (MQ=255)                                                                                                                                                                                                                         aTCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATg                       <  1:266547/251‑1 (MQ=255)                                                                                                                                                                                                                                              ccatttACTGTCGTCGGAGCAAACGGCAATCGTTAATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTcacc  <  2:153865/247‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 GCCCAGCGCAATGCTGATATTCGGGTTAATGCCCTGACCGTTAAAGCCCGTCGCGGCGATCACCACGCCGACAAAAGCCG‑CGCTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAA‑CATCGCCACCGCGTGTTGAACGCCCATTACTGCCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACC  >  NC_000913/1069498‑1069983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑