Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	246,747	G→T	S12S (TCG→TCT)	yafL →	NlpC/P60 family protein YafL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	246,747	0	G	T	100.0%	88.4 / NA	27	S12S (TCG→TCT)	yafL	NlpC/P60 family protein YafL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (17/10);  total (17/10)

CAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTG‑TTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTA‑TTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACC‑AAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAAT  >  NC_000913/246532‑246973                                                                                                                                                                                                                          |                                                                                                                                                                                                                                    cAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTG‑TTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTA‑TTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCAtt                                                                                                                                                                                                  <  1:247177/251‑1 (MQ=255)  aGCTTGAATTGTAACTCAAATGAGCAACAGCGACACTCCCTGCGTATTTTAATAAACGGCTATTTTTACATAATTCACTGACATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTG‑TTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTA‑TTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCCttt                                                                                                                                                                                                 >  2:407206/1‑251 (MQ=255)     ttGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTG‑TTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTA‑TTGTCTGGATTACTGtt                                                                                                                                                                                                                          >  1:206488/1‑223 (MQ=255)     ttGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTG‑TTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTA‑TTGTCTGGATTACTGtt                                                                                                                                                                                                                          <  2:206488/223‑1 (MQ=255)                                                 tttAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTG‑TTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTA‑TTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCcgcccgcc                                                                                                                                                   >  2:356897/1‑250 (MQ=255)                                                       aaaCGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTG‑TTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTA‑TTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGAt                                                                                                                                            >  1:120539/1‑251 (MQ=255)                                                                   ttACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTG‑TTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTA‑TTGTCTGGATTACTGTTGCTTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATctct                                                                                                                                                                      >  2:217535/1‑213 (MQ=255)                                                                   ttACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTG‑TTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTA‑TTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATctct                                                                                                                                                                      <  1:217535/213‑1 (MQ=255)                                                                                     tatatatGTTAATTTTATAATAATGGTTTATTAAGTTTG‑TTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTA‑TTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGtc                                                                                                              >  1:321224/1‑250 (MQ=255)                                                                                      atatatGTTAATTTTATAATAATGGTTTATTAAGTTTG‑TTCAGAACCGATACGGGATACACTGCAACATTACAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTA‑TTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACCTATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCACGTTTTTTAAAACAGTAc                                                                                                             >  2:411604/1‑251 (MQ=255)                                                                                            gTTAATTTTATAATAATGGTTTATTAAGTTTG‑TTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTA‑TTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACC‑AAACt                                                                                                      <  1:13304/251‑1 (MQ=255)                                                                                            attAATTTTAAAATAATTGTTTAT‑AAGTTTGGTTCAGAAACGACCCGGGATACACGTAAATATTAAAGCATGACCAGCCATTATTGAGTATGTCTTTGCCGTCGATTCCATCCTTTGTAGTTG‑CTGGATTACTGTTGATTGGTTTGCCGTTTACGTCATTTGCCGGCGCCACCACATCACATATCGCTTTCAGCTAGGCCGCCCGCCAGCGGATGCAAAACCGTTCGCGTTTATTAAAACATTACC‑AAACt                                                                                                      <  2:424530/250‑1 (MQ=255)                                                                                                                     aaGTTCG‑TTCAGAAACGATCCCGGATACACTGCAACCTTCACGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGCTTCCATCATTTGTC‑TTGACTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCAAGCGCCACCACATCACATATCTCTTTCAGCTACGACGACCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAACCAGTACA‑CAACTCATCTGAAAAAGCAGGcacgctatt                                                                             >  2:224341/1‑243 (MQ=255)                                                                                                                       gTTTG‑TTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACATGCCATTATTGAGTATGTCCCTGCCGTCGCTTCCAACCTTTGTA‑TTGCCTGGCTTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGAATGCAAAACCGTGCGCGTTTATTAAAACAGTACC‑AAACTCATCTGAAAAAGCAGGCCAGCTATAtt                                                                           >  1:37846/1‑251 (MQ=255)                                                                                                                                          cGGGATACACTGCAACATTAAAGCATGAACAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTA‑TTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCCAAACCGTGCGCGTTTATTAAAACAGAACC‑AAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGACGGCAATGCCGa                                                          >  2:326477/1‑250 (MQ=255)                                                                                                                                                   cTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTA‑TTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCCTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACC‑AAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCaaca                                                  >  1:370021/1‑247 (MQ=255)                                                                                                                                                     gCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTA‑TTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACC‑AAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAgg                                                <  1:371288/249‑1 (MQ=255)                                                                                                                                                         cATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTA‑TTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACC‑AAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCt                                           >  1:65950/1‑250 (MQ=255)                                                                                                                                                            tAAAGCATGACCAGCCATTATTGAGTCTCTCCTTGCCGTCGATTACCTCCTTTGTA‑TTGTCTGGCTTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTATTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAACCA‑TACCCAAACTCATCTGAAAAAGCAGGCCAGATATATTGTGGAAAGCAATGCCGAAAGCAAAAGGGCGCTaagc                                       >  2:275084/1‑248 (MQ=255)                                                                                                                                                                  aTGACAAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTA‑TTGTCTGGATTCCTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACC‑AAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGcac                                 >  2:227296/1‑251 (MQ=255)                                                                                                                                                                            attatgtagattgtcttggCCGGCGATTCCATCCTTTGTA‑TGGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCCCATCTCATACCTCTTTCAGCTCCGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACC‑AAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAg                        <  2:119475/232‑1 (MQ=255)                                                                                                                                                                               attGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTA‑TTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACC‑AAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGc                       >  1:227035/1‑248 (MQ=255)                                                                                                                                                                               attGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTA‑TTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACC‑AAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGa                    <  2:120539/251‑1 (MQ=255)                                                                                                                                                                                  gAGTATGTCCTTGCCGTCGATTCCATCCTTTGTA‑TTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACC‑AAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATa                  >  2:16328/1‑250 (MQ=255)                                                                                                                                                                                          ccTTGCCTGCGATTCCATCCTTTGTA‑TTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACC‑AAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCa          <  2:76510/250‑1 (MQ=255)                                                                                                                                                                                          ccTTGCCGTCGATTCCATCCTTTGTA‑TTGTCTGGATTCCTGTTGTTTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACC‑AAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAACACAGCa          <  1:59675/250‑1 (MQ=255)                                                                                                                                                                                                 gTCGATTCCATCCTTTGTA‑TTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACC‑AAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAAt  >  2:325994/1‑251 (MQ=255)                                                                                                                                                                                                                          |                                                                                                                                                                                                                                    CAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTG‑TTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTA‑TTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACC‑AAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAAT  >  NC_000913/246532‑246973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑