Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	968,837	G→A	D158N (GAT→AAT)	lpxK →	tetraacyldisaccharide 4'‑kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	968,837	0	G	A	100.0%	145.4 / NA	47	D158N (GAT→AAT)	lpxK	tetraacyldisaccharide 4'‑kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/21);  total (26/21)

GGTCGTATCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTGATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCG‑CTTA‑AAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTG‑CCGGG‑TCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGC  >  NC_000913/968605‑969083                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                           ggTCGTATCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTa                                                                                                                                                                                                                                          <  1:260181/251‑1 (MQ=255)                       ggtAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGTAACTTTGTTGTTTTATCAACGCACTGTTGCGCCCGTTTCGGGTTCTCCCGGTTGTTCTGATGCGGGAAAAACCATTCTGGAGCAAAACCCCTATGTGCAGATCATCGTAACCCAAGACGGTTTACAGCATTACCGTCTGGGGCGTAATGTGGGAATTTTCGTTTTTGGTAGGGTGCGCCGCTTTgg                                                                                                                                                                                                                    >  2:335307/1‑251 (MQ=255)                                    cTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATTGACCTCTGTTTGTTTATTAACGCACCGCTGCGCCTGTTTCGGTTTTTCCCGCTCGGTCTGTTGCGGTAAAAGCCCTTCTGGCGCAACACCCTGATGTGCCGATCATCGTAACCGACGACGGGTTTCAGCATTACCCGCTGGCGCGTAATGTGGAAATTTGCGTTTTTGATGGGGTGCGGCGCTTTGGCAATGGCtggggg                                                                                                                                                                                                       >  2:99865/1‑248 (MQ=255)                                      tATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCtggtg                                                                                                                                                                                                        <  2:18461/248‑1 (MQ=255)                                         ccGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTGCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGcc                                                                                                                                                                                                  <  2:256636/251‑1 (MQ=255)                                             tGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGGTTCTCCCGTTCGTTCTGATGCGGTAAAAACCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGGTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGTTGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCcggcg                                                                                                                                                                                              >  1:106170/1‑251 (MQ=255)                                              gTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCCACGCAATGATGCGCCTGTTGCGGGTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGGTTACAGCATTAACGTCTGGCGCGTAATGTGGAAATTTTCGTTATTGATGGGGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCcggcgg                                                                                                                                                                                             >  1:67446/1‑251 (MQ=255)                                                 aTTGTCGGCAGATACCACAACAGCGCAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGAGGCGCCTGTTGCGGTTTCTCCCGTTCGGTCTGATGCGGGAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGGCTGGCGCGTAATGTGGAAATTGTCGTCATTGATGGTGTGTGTCGCGTTGGCAATTGCTGGTGGTTGTCGGCaggg                                                                                                                                                                                           <  2:317262/250‑1 (MQ=255)                                                 aTTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTTATTTATCAACCCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTTTGATGCGGTAAAAGCCATTTTGGCGCAACCCCCTGATGTGCAGATCATCGTAACAGACGGCGGTTTTCAGCATTACCGTCTGGCGCGTAATGTGGGAATTGTCGTTATTGGTGGTGTGCGGCGCTTTGGCCATGGCTGGGGGTTGGCGggggggg                                                                                                                                                                                          >  2:112410/1‑250 (MQ=255)                                                         cAGATATCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGGTGCCGGCGGGGCCAATGCGt                                                                                                                                                                                  <  1:365188/251‑1 (MQ=255)                                                                cacaACAGCACAGGCGGGTGGTGAGCCTGGGTTGATTTATCCACGCCCTGATACGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTCAAAGCCCATCTTGCGCAACACCCCGATGGGCAGATTATCTTAACCGACCAAGGTTTACCGCATTACCGTCTGGGGCGTAATGCGGTAATTGTCGTTATTGCTGGTGTGCGTCGATTTTGCAATGTCTGGTGGTTGCCCGCGGGGcccacgagggggggcg                                                                                                                                                                           >  2:245730/1‑237 (MQ=255)                                                                    acaGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCGGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCggg                                                                                                                                                                       <  2:92014/251‑1 (MQ=255)                                                                     caGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCgggg                                                                                                                                                                      <  1:18418/251‑1 (MQ=255)                                                                           aGGCGGGTGATGAAACTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGAAATTCTGGCGCAACACCATGATGTGAAGATCATCGTAACCGACGACGGTTTACAGCCTTACAGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGGCAATGGGTgggggggcggggggggt                                                                                                                                                                 >  1:62127/1‑234 (MQ=255)                                                                                           tgtgTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTCAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAAAGCGTGAGCCAGCGGGGCG‑GTTA‑AAGTCgcgtggtgcgg                                                                                                                                              >  2:116675/1‑241 (MQ=255)                                                                                                     tATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGAGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGGGGGCGAGCGGGGCG‑CTTA‑AAGTCGGTTGGTGCGGTAATCGTCa                                                                                                                                     >  1:268887/1‑250 (MQ=255)                                                                                                             cACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCCATGCGGGAGGGCGCGGGGCGGCTTA‑A‑GGCGGGTGAGGCGGGAATCGCCAAcggggggg                                                                                                                            >  2:154696/1‑246 (MQ=255)                                                                                                             cACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCG‑CTTA‑AAGTCGGTTGATGCGGTAATCGTCAACGGCGgtg                                                                                                                            <  2:106170/251‑1 (MQ=255)                                                                                                             cACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCG‑CTTA‑AAGTCGGGTGATGCGGTAATCGTCAACGGCGgtg                                                                                                                            >  1:225003/1‑251 (MQ=255)                                                                                                                 gATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGGGGGCGAGCGGGGCG‑GTTA‑AAGTCGGTTGGTGCGGGAATGGTCAACGGCGGAGTccc                                                                                                                        >  1:36113/1‑251 (MQ=255)                                                                                                                      gcCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCG‑CTTC‑AAGTCGGTTGATGCGGTAATCGTCAAcggcggggtgccccgcc                                                                                                                   >  2:164985/1‑240 (MQ=255)                                                                                                                      gcCTGTTGCGGTTTCTCCCGTTCATTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTACTGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGGGGGGCG‑CTTA‑AAGTCGGTTGGAGGGGTAATCGTCAAAGGCGGTGTTCCTCGcc                                                                                                                   >  1:126672/1‑250 (MQ=255)                                                                                                                          gTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCTCAACACCCTGATGTGCAGATCATCGTACCCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCG‑CTTA‑AAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGc                                                                                                                 <  1:375556/249‑1 (MQ=255)                                                                                                                               ggTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCG‑CTTA‑AAGTCGGTTGATGCGGTAATCGTCACCGGCGGTGGCACTCGCAGCGGGGaa                                                                                                           >  1:147991/1‑250 (MQ=255)                                                                                                                                   tctcCCTTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTTCAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGTGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCTGGGCCAATGCGTGAGCGAGCGGGGCT‑CTTA‑ATGTCTGTTGATGCTGTAAGCGTCAACTGCGGTGTCCCTCTCAACGGTGAAATcc                                                                                                       <  2:445675/250‑1 (MQ=255)                                                                                                                                         gttcgttcTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCG‑CTTA‑AAGTCGGTTGATGCGGTAATCGTCAACGGCGGGGGTCCTCGCAGCGGGGAAATCCCCCTGcc                                                                                                >  1:318964/1‑250 (MQ=255)                                                                                                                                          ttcgttcTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCG‑CTTA‑CAGTCGGTTGATGCGGGACTCGTCCAAGGCGGGGTGCCCCGCACCGGTGAAAAcccccagact                                                                                               >  2:411521/1‑245 (MQ=255)                                                                                                                                            cgttcTGATGCGGTAAAAGCCATGCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCG‑CTTA‑AAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATct                                                                                             <  2:67446/251‑1 (MQ=255)                                                                                                                                                 tGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCG‑CTTA‑AAGTTGGTTGATGCGGTAATCGTCAACGGCGGGGTCCCTCGCCGCGGTGAAAATCCCATGCAttttctgg                                                                                        >  1:102629/1‑244 (MQ=255)                                                                                                                                                      cGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCG‑CTTA‑AAGGCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTG‑CCGGG‑t                                                                                 <  2:36113/251‑1 (MQ=255)                                                                                                                                                             aGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCG‑CTTA‑AAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTG‑CCGGG‑TCAGGCgg                                                                          <  1:365434/251‑1 (MQ=255)                                                                                                                                                                cATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGTGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCG‑CTTA‑AAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTG‑CCGGG‑TCAGGCGTTGa                                                                       <  2:393163/251‑1 (MQ=255)                                                                                                                                                                 aTTCTGGCGCAACACCCTGCTGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGGG‑CTTG‑AAGTCGGGTGGTGCGGTAATCGTCAACGGCGGGGTGCCTCGCCGCGGGGAAAACTCCATgaacctggggccggggcggggggggg                                                                         >  2:270933/1‑225 (MQ=255)                                                                                                                                                                 aTTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGAAGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAACTTGTCGTTATTGATGGTGCGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGACACTGCGTGATCGAGCGGGGCG‑ATTA‑AAGTAGGTTTAATCGGGAAGCGTCAACGGCGGGGGACCTCACAGCGGTGAACTTCCCACGCAACTACTG‑CCCGG‑ACAgggggag                                                                        >  1:261954/1‑244 (MQ=255)                                                                                                                                                                   tCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCG‑CTTA‑AAGTCGGTTGATGCGGTAATCGTCAAAGGCGGGGTCCCCCGCCGCGGGGGAATCCCCCCGCATCTGCTGTCCgggccggcgggggatt                                                                      >  2:173764/1‑238 (MQ=255)                                                                                                                                                                    cTGGCGCAACACCCTGATGTGCAGATCATCGTCACTGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGGGGGCGAGCCGGGGG‑CTTATAAG‑CGGTTGAGTCGGGAATCGTCAACGGCGGGGTCCCTCCCAGAGGGGGAATCTCCCCGAATCTGCTG‑CCGGG‑Tccagcgggggattt                                                                   >  2:117127/1‑238 (MQ=255)                                                                                                                                                                      ggCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCG‑CTTA‑AAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTG‑CCGGG‑TCAGGCGGTGAAttt                                                                   <  1:164985/249‑1 (MQ=255)                                                                                                                                                                                 cTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCG‑CTTA‑CAGTCGGTGGATGCGGTAATCGTCAACGGCGGAGTCCCTCGCAGCGGTGAAATTCCCAAGCCACCGCTG‑CCGGGGTCAGGCGGGGAATTTACGTACCgaggc                                                       >  1:71610/1‑247 (MQ=255)                                                                                                                                                                                       tgCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGAAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGAAATGGCTGGAGGTTGCCGGCGGGGGCAATGCGTGAGGGAGCGGGGGG‑CTTA‑AAGTCGGGTGATGCGGTAATAGTCAACGGCGGTGGCCCTCGCAGCGGGGGAATTACCAAGCCTCTGCTG‑CCGGG‑TCAGGCGGTGAAATTACGTAACGGGACGGGTtgg                                                >  1:253977/1‑250 (MQ=255)                                                                                                                                                                                          aGATCATCGTTACCGACGCCGTTTTACAGCATTACCGTTTGGTGCGTAATGTGGAAATTGTCGTTATGTATGGTGTGCGTCGTTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCT‑CTTA‑AAGTCGGTTTATGCGGTAATCGTCAACGTCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTG‑CCGGG‑TCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGAc                                             <  1:257728/251‑1 (MQ=255)                                                                                                                                                                                          aGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCG‑CTTA‑AAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTG‑CCGGG‑TCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGAc                                             <  1:279239/251‑1 (MQ=255)                                                                                                                                                                                                             cgGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCG‑CTTA‑AAGTCGGTTGATGCGGTACTCGTCAACGGCGGTGTCCCCCGCCGCGGGGGAATTCCCCTGCATCTGCTG‑CCGGG‑GCCGGCGGGGGATTTTCCTAACGGGACGCGTtgggggcgggagggcccccggctgg                          >  1:62744/1‑228 (MQ=255)                                                                                                                                                                                                             cgGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCG‑CTTA‑AAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTG‑CCGGG‑TCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATg                          <  1:32887/251‑1 (MQ=255)                                                                                                                                                                                                             cgGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCG‑CTTA‑AAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCGGCTG‑CCGGG‑TCAGGCGGTGAATTTACGTACCGGTACGCGTtg                                                 <  2:62744/228‑1 (MQ=255)                                                                                                                                                                                                                              gTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGGGAGCGAGCGGGGCG‑CTTA‑AAGTCGGTTGATGCGGGAATCGTCAACGGCGGGGTCCCCCGCCGCGGGGAAATACCCCTGCCTTTGCTG‑CCGGG‑GCAGGCGGTGGATTTACCTACCCGTACGCGGTGTGAAGGTGCTCCGCTTTAAAAAGGAGTGGCGGTGGCgggg         >  2:139763/1‑251 (MQ=255)                                                                                                                                                                                                                                  ggCGCGTAATGTGGAAATTGGCGTTATTGATGGTGTGCGTCGCTGTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCG‑CTTA‑AAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTG‑CCGGG‑TCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGAtt      <  2:78376/250‑1 (MQ=255)                                                                                                                                                                                                                                     gcgTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCG‑CTTA‑AAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTG‑CCGGG‑TCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGc  <  1:112410/251‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                           GGTCGTATCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTGATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCG‑CTTA‑AAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTG‑CCGGG‑TCAGGCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGC  >  NC_000913/968605‑969083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑