Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,532,409	T→G	intergenic (‑758/+28)	sgcX ← / ← yjhP	putative endoglucanase with Zn‑dependent exopeptidase domain/putative methyltransferase YjhP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,532,409	0	T	G	100.0%	208.7 / NA	56	intergenic (‑758/+28)	sgcX/yjhP	putative endoglucanase with Zn‑dependent exopeptidase domain/putative methyltransferase YjhP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (37/19);  total (37/19)

AACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTTCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCG  >  NC_000913/4532169‑4532653                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     aaCCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGa                                                                                                                                                                                                                                             >  1:299811/1‑250 (MQ=255)  aCCTGATACCACTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCCCTGGCACTGTGTGCATCCGGTGCCGACACCGCCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTACTCAACATACTGAATATGTATTGCGCAGGTGATACATCAGATCAAAGCAAAGGCAAAGCCTCGATATTGCCAGCCAGaa                                                                                                                                                                                                                                            >  1:289652/1‑250 (MQ=255)   ccTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAAt                                                                                                                                                                                                                                          <  1:194228/251‑1 (MQ=255)      gATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATAc                                                                                                                                                                                                                                        <  1:95355/250‑1 (MQ=255)                  tcCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCtgtt                                                                                                                                                                                                                            <  1:101972/250‑1 (MQ=255)                          tttGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCggg                                                                                                                                                                                                                   >  1:331090/1‑251 (MQ=255)                           ttGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGc                                                                                                                                                                                                                  >  2:392909/1‑251 (MQ=255)                              agaTTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGAAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGGTACCGGGCGAt                                                                                                                                                                                                               >  2:248380/1‑251 (MQ=255)                                     cGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCg                                                                                                                                                                                                        <  1:228980/251‑1 (MQ=255)                                         gCTGTCGCTGCATTACGCACAATATTGAGCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCATAAATACCGTGAACCTTTTGTTACCGGGCGATGAGTGCGAaca                                                                                                                                                                                                    <  1:197837/251‑1 (MQ=255)                                                cTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGcccc                                                                                                                                                                                              >  1:395484/1‑250 (MQ=255)                                                 tgaaTTACGCACAATATAGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGGTACCGGGCGATGAGTGCGAACACGcccc                                                                                                                                                                                              >  2:240147/4‑249 (MQ=255)                                                       aCGCACAATATTGATCACTGGCACTGAGTGCATCTGGTGCCGACATCGACATTCCGCAAGCCTGACAGCACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAACGCATCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGCGCGAACACGCCCCAGCCaa                                                                                                                                                                                        >  2:415283/1‑249 (MQ=255)                                                            caATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTc                                                                                                                                                                                 >  1:27766/1‑251 (MQ=255)                                                                 ttGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGCCAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGCGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGCTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCAGTTGTTACCGGGCGATGAGTGCGAACACCCCCCAGCCAACACATTCCcgcg                                                                                                                                                                            >  2:15356/1‑251 (MQ=255)                                                                  tGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCcgcgc                                                                                                                                                                           >  2:367177/1‑251 (MQ=255)                                                                      cACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAg                                                                                                                                                                       <  1:126424/251‑1 (MQ=255)                                                                             aCTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGt                                                                                                                                                                >  1:214091/1‑251 (MQ=255)                                                                                  gtgCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGt                                                                                                                                                           <  2:331090/251‑1 (MQ=255)                                                                                              ccGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATg                                                                                                                                               >  1:386153/1‑251 (MQ=255)                                                                                               cGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAg                                                                                                                                                              <  1:63303/235‑1 (MQ=255)                                                                                               cGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAg                                                                                                                                                              >  2:63303/1‑235 (MQ=255)                                                                                                 aCATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAGCCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCTGCGCGATGttt                                                                                                                                            <  2:192705/251‑1 (MQ=255)                                                                                                                             cTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGt                                                                                                                >  2:106409/1‑251 (MQ=255)                                                                                                                                     gttgtGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCa                                                                                                        <  1:14622/251‑1 (MQ=255)                                                                                                                                       tgtgACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGAAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTAAgg                                                                                                      >  1:216350/1‑251 (MQ=255)                                                                                                                                         tgACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGAt                                                                                                    >  2:358248/1‑251 (MQ=255)                                                                                                                                            cATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCg                                                                                                 >  1:16936/1‑251 (MQ=255)                                                                                                                                                       ccTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTccagcca                                                                                       <  1:147310/250‑1 (MQ=255)                                                                                                                                                       ccTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTccagcc                                                                                        >  1:158107/1‑249 (MQ=255)                                                                                                                                                              gTGACATCTTGATTAATAAACATACTGAATATGTATTGGGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATAGTGCCAGCCAGAAATACCGGGAACCTGTTGTGGCCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGCCTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCa                                                                               <  2:299811/251‑1 (MQ=255)                                                                                                                                                                 aCATCTTGATTAATAAACATACTGAATATGTCTTGTGCAGGTGAAAAATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAACTACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGAGTTTCGGCGCGATGATTAGTTCGGCCCTGACCTCGGCGGCGAAGTCATCGTCAGGATTCGCCTCCAGCCAGCGGCGCATgg                                                                            >  1:407455/1‑251 (MQ=255)                                                                                                                                                                         aTTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCAt                                                                    >  1:260907/1‑251 (MQ=255)                                                                                                                                                                         aTTAATAAACATACTGAATATGTATTGGGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATTCCGGGAACCTGTTGTGACCGGGTGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTCCTGCGCGATTTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCAt                                                                    <  1:408247/251‑1 (MQ=255)                                                                                                                                                                           taataaACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCAttt                                                                  >  1:99219/1‑251 (MQ=255)                                                                                                                                                                            aataaACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCAttt                                                                  <  1:84064/250‑1 (MQ=255)                                                                                                                                                                             ataaACATACTGAATATTGATTTTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCATCGGCGCATGGTCGGCCATTTTg                                                                <  2:251068/251‑1 (MQ=255)                                                                                                                                                                             ataaACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTg                                                                >  1:178072/1‑251 (MQ=255)                                                                                                                                                                                 aCATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGc                                                            >  1:170059/1‑251 (MQ=255)                                                                                                                                                                                 aCATACTGAATATGTATTGTGCAGGTGATACATAAGATCAAAGCAAAGGTAAAGCCTCGATATGGCCAGCCAGAAATACCGGGAACCTGTTTTTACCGGGCGATGAGTGCGAACACGACCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCCGGATTCACTTCCACCCAGCGGCGCATGGTCAGCCATTTTGCGGc                                                            >  2:14375/1‑251 (MQ=255)                                                                                                                                                                                       tGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCAt                                                       >  1:39023/1‑250 (MQ=255)                                                                                                                                                                                        gAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAACGGAAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGCCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGACAGCCATTTTGCGGCTTCATAc                                                     >  2:286225/1‑251 (MQ=255)                                                                                                                                                                                        gAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATa                                                      <  1:124113/250‑1 (MQ=255)                                                                                                                                                                                         aatatgaaatgagCAGCTGATACATCCGATCAAAGCAACGGGAAAGCCTCGATATTGCCAGCAAGAAATACCGGGAACCTGTTGTTACCGGGCGCTGAGTGCGCACACGCCCCAGCCACAGCATTCCCGCGCGTAAGTCACGTAGCGTTTCGGCGCGACGCTTACTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTACAGACTGCGGCGCCTGGTCAGCCATTTTACGGCTTCATAcc                                                    >  2:279942/13‑251 (MQ=255)                                                                                                                                                                                           tatGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCgg                                                  >  1:243131/1‑251 (MQ=255)                                                                                                                                                                                           tatGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCgg                                                  >  1:255911/1‑251 (MQ=255)                                                                                                                                                                                               tATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACATGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGGCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCCTACCGGTccc                                              >  2:32938/1‑251 (MQ=255)                                                                                                                                                                                                   gtgCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATAGTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATGCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGc                                           <  2:178072/250‑1 (MQ=255)                                                                                                                                                                                                    ggCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCt                                         <  1:418822/250‑1 (MQ=255)                                                                                                                                                                                                      cAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTGCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTc                                       <  1:148264/251‑1 (MQ=255)                                                                                                                                                                                                          tGATACATAAGATCAAAGCACCGGTAAAGCCTCGATATTGCCAGCCAGAACTAGCGGGAACCTGTTGTTACCGAGCGATGAGTGCGAACACGCCCCAGCCAACGCATTCCCGCGCGTAGGTACCGTAGCGTTTCGGCGCGAAGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGACAACCATTTTGCGGCTTCATACCGGTCCCCGCCTTCCTgc                                   >  2:430016/1‑250 (MQ=255)                                                                                                                                                                                                           gATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGt                                  >  2:232728/1‑251 (MQ=255)                                                                                                                                                                                                                 tCAGATCAAAGCAAAGGTAAAGCCTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGcc                             >  1:80034/1‑250 (MQ=255)                                                                                                                                                                                                                                   aaaGCCTCGATATTGCAAGACAGAAATACCGGGAACCTGTCGTTACCGGCCGATGAGTGCGAACACGCCCCAGCCAAAGCCTTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTACCAGCCTTCCTGGTCTGCCCGTACCATTTcctccccgt          >  2:280035/1‑244 (MQ=255)                                                                                                                                                                                                                                        cTCGATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTCGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCCTACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCAt       >  2:257092/1‑249 (MQ=255)                                                                                                                                                                                                                                           gATATTGCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCCTACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCg  >  2:6280/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     AACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTGACATATGATGCTCCTTATGGTGACATCTTGATTAATAAACATACTGAATATGTATTGTGCAGGTGATACATCAGATCAAAGCAAAGGTAAAGCCTCGATATTTCCAGCCAGAAATACCGGGAACCTGTTGTTACCGGGCGATGAGTGCGAACACGCCCCAGCCAAAGCATTCCCGCGCGTAGGTCACGTAGCGTTTCGGCGCGATGTTTAGTTCGGCCCTGACTTCGGCGGCGAAGTCATCGTCAGGATTCGCTTCCAGCCAGCGGCGCATGGTCAGCCATTTTGCGGCTTCATACCGGTCCCAGCCTTCCTGGTCTGCCAGTACCATTTCCACCACGTCATAGCCG  >  NC_000913/4532169‑4532653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑