Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,499,661	T→C	intergenic (+161/‑1599)	insD6 → / → yjgZ	IS2 insertion element protein InsB/uncharacterized protein YjgZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,499,661	0	T	C	100.0%	113.1 / NA	30	intergenic (+161/‑1599)	insD6/yjgZ	IS2 insertion element protein InsB/uncharacterized protein YjgZ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/16);  total (14/16)

GCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCG  >  NC_000913/4499430‑4499884                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                gctcgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGGCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTCAAAAACCATAACAGgttag                                                                                                                                                                                                              <  1:92783/251‑1 (MQ=255)    cgcCACGGGAATATCTGCGGCAGCGGGCGTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTAACTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTCAAAAACCATAACAGgttagtt                                                                                                                                                                                                            <  1:304067/250‑1 (MQ=255)               tatCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGATTACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTCAAAAACCATAACAGGTTAGTTATCTTCGTCTGt                                                                                                                                                                                                >  2:43615/1‑251 (MQ=255)                         aGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTCAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTg                                                                                                                                                                                      <  1:48715/251‑1 (MQ=255)                            gggCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTCAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAAt                                                                                                                                                                                   >  2:6718/1‑251 (MQ=255)                             ggCTTGTAATGGGTTAAGTGATAACAGCTGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTCAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATg                                                                                                                                                                                  >  2:387466/1‑251 (MQ=255)                                     aTGGGTTACGTGATAACAGATGACTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATAAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCACTCAAAAACCATAACAGGTTCGTTATCTGCTTCTGTCAGCGGATTGAATGGAGGtat                                                                                                                                                                           >  1:338083/1‑250 (MQ=255)                                                       gATGTCTGGAAATATAGGGGAAAATCCACCTTGTGCTGATATGTATACGGATATTTTATATAGAATACTTTTCAGTACAAGCGACTGTATGACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGGTTCAATCTGTCTGCCGGTATATTCCTCTCTCCCTCAAAAACCATAACAGGTTAGGTAGCTTCGTCTGTCAGCGGAGTGAATGGCGGTATATTTTCAGTACAAATACCg                                                                                                                                                        <  2:54199/251‑1 (MQ=255)                                                         tGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTCAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTa                                                                                                                                                                 >  2:126941/1‑240 (MQ=255)                                                         tGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTCAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTa                                                                                                                                                                 <  1:126941/240‑1 (MQ=255)                                                              ggAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTCAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATcc                                                                                                                                                 >  1:201757/1‑251 (MQ=255)                                                                                 cACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTCAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGaa                                                                                                                              <  1:401547/251‑1 (MQ=255)                                                                                    cTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTCAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATg                                                                                                                           >  2:410675/1‑251 (MQ=255)                                                                                      tgtgCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTCAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATg                                                                                                                           <  1:410675/249‑1 (MQ=255)                                                                                               atGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTCAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGTCACTg                                                                                                                 <  1:138601/250‑1 (MQ=255)                                                                                                 gAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTCAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGAt                                                                                                               <  1:362596/250‑1 (MQ=255)                                                                                                   aTACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTCAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGAtttt                                                                                                            <  1:444109/251‑1 (MQ=255)                                                                                                              ttATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTCAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACgg                                                                                                 >  1:60766/1‑251 (MQ=255)                                                                                                                          cTTTTAAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTCAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCCGCGGATTGAATGGAGGTATTTTTTCAGTACAAATACCGGTCATCCAATGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGATGATGAGACg                                                                                     >  1:290725/1‑251 (MQ=255)                                                                                                                            tttCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTCAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGCACTGACGGGCACTGATTTTTCTACTGACGGGCAGATGGGACGtt                                                                                   >  2:20362/1‑251 (MQ=255)                                                                                                                                   aCAAGCGCCTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGCAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTCAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGAATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGCCGGGCTGATGAGACGTTATGTCAc                                                                            >  2:243114/1‑251 (MQ=255)                                                                                                                                     aaGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTCAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGGTATGTCACTg                                                                          <  1:129761/251‑1 (MQ=255)                                                                                                                                                           cATATATGATTACCGGTGAGCAGGAAGAATAAGTGGGTGAGGGTTCAAACTTTCTGCCGGTATATTCCTCTCTCCCTCAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCTGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTTGCacac                                                    <  2:142978/251‑1 (MQ=255)                                                                                                                                                                     tACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTCAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGATATAATctaggggcccatgtggcctcc                                          >  2:200378/1‑231 (MQ=255)                                                                                                                                                                                     gaaTAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTCAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATaa                          <  1:390352/251‑1 (MQ=255)                                                                                                                                                                                                      ttCAAACTGTCTGCCGGTATATTCCTCTCTCCCTCAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTgg          <  1:387466/250‑1 (MQ=255)                                                                                                                                                                                                             tGTCTGCCGGTATATTCCTCTCTCCCTCAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCCGTGGCACCCTGTAGCCTCCCCGCCCCTTACCATAATTATTAACAGGATTTGCCCTGTCg  >  1:442933/1‑251 (MQ=255)                                                                                                                                                                                                              gTCTGCCGTTATATTCCTCTCTCCCTCAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTc   <  1:258736/249‑1 (MQ=255)                                                                                                                                                                                                                           ttCCTCTCTCCCTCAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGAtt                                                                                                              <  2:337919/129‑1 (MQ=255)                                                                                                                                                                                                                           ttCCTCTCTCCCTCAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGAtt                                                                                                              >  1:337919/1‑129 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                GCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCG  >  NC_000913/4499430‑4499884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑