Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	109,960	G→A	R561H (CGT→CAT)	secA →	protein translocation ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	109,960	0	G	A	100.0%	141.4 / NA	42	R561H (CGT→CAT)	secA	protein translocation ATPase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/26);  total (16/26)

GAACGCCAAATTCCACGCCAACGAAGCGGCGATTGTTGCTCAGGCAGGTTATCCGGCTGCGGTGACTATCGCGACCAATATGGCGGGTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAAATTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCGTCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATGCGCTGATGCGTATTTTTGCTTCCGACCGAGTATCCGGCA‑TGATGCGTAAACTGGGTATGAAGCCAGGCGAAGCCATTGAACACCCGTGGGTGACTAAAGCGATTGCCAACGCCCAGCGTAAAGTTGAAAGCCGTAACTTCGACATTCGTA  >  NC_000913/109715‑110205                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       gAACGCCAAATTCCACGCCAACGAAGCGGCGATTGTTGCTCAGGCAGGTTATCCGGCTGCGGTGACGATCGCGACCAATATGGCGGGTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAAAGTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCGGCATATCATCTGTACCGAGCATCACg                                                                                                                                                                                                                                                   <  2:72133/251‑1 (MQ=255) gAACGCCAAATTCCACGCCAACGAAGCGGCGATTGTTGCTCAGGCAGGTTAGCCGGCTGCGGTGACTATCGCGACCAATATGGCGGGTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAAAGTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCTAGCATCACg                                                                                                                                                                                                                                                   <  2:443008/251‑1 (MQ=255)                               gATTGTTGCTCAGGCAGGTTATCCGGCTGCGGTGACTATCGCGACCAATATGGCGGGTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAAATTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCATCACGAATCCCGTCGTATCGATAACCAGTTGcgcg                                                                                                                                                                                                                     <  1:419357/251‑1 (MQ=255)                                         caggcaggTTATCCGGCTGCGGTGACTATCGCGACCAATATGGCGGGTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAAATTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCATCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTg                                                                                                                                                                                                            <  1:71859/250‑1 (MQ=255)                                          aggcaggTTATCCGGCTGCGGTGACTATCGCGACCAATATGGCGGGTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAAATTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCATCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGgt                                                                                                                                                                                                          <  1:160280/251‑1 (MQ=255)                                          aggcaggTTATCCGGCTGCGGTGACTATCGCGACCAATATGGCGGGTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAAATTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCATCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGgt                                                                                                                                                                                                          <  1:161239/251‑1 (MQ=255)                                           ggcaggTTATCCGGCTGCGGTGACTATCGCGACCAATATGGCGGGTCGTGGGGCAGATATTGTGCTCGGGGGGAGCTGGCGGGCAGAAGGTGCCGCGCCGGGAAATCCGACCGCCGGGCAAATTGAAAAAATTAAAGCCGACCGGCAGGGGCGTCACCAAGCGGGACTGGGAGCAGGGGGCCCGCATATCCTCGGTGTCGAGGATCACGAATCCCCTCGTATTGAAAACCCGTTGCGCGGGCGTTTTGgtg                                                                                                                                                                                                         >  1:348366/1‑250 (MQ=255)                                                           gCGGTGCCTATCGCGACCAATATAGCGGGTCGTGGTACAGAGAATGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTTCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAAATTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGGGGCCTGCATATCATCGGTACCGAGCATCACGTATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCt                                                                                                                                                                                           <  2:302562/249‑1 (MQ=255)                                                                                atGGCGGGTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAAATTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCATCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGt                                                                                                                                                                     <  1:158947/250‑1 (MQ=255)                                                                                atGGCGGGTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAAATTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCATCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTc                                                                                                                                                                    <  1:119173/251‑1 (MQ=255)                                                                                 aggagggTCGTTGTACACATATTGTGCTGTGTGGGTGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAAATTAAAGCCGATTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCATCACGAAGCCCGTCGTATCTATATCCATTTGCGCGGTCGTGCTCGTCGCCAGGCGGATGCTGGTGCTTCCCGTTTcggccggtct                                                                                                                                                                   <  2:267741/247‑10 (MQ=255)                                                                                     gggTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAAATTAAAGCCGACTGGCAGGTACGTCACGCTGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCATCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGGCGTTCTGGTCGTCCGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATgg                                                                                                                                                               >  2:168635/1‑251 (MQ=255)                                                                                     gggTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAAATTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCATCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTGCCCGTTTCTACCTGTCGATgg                                                                                                                                                               <  1:100958/251‑1 (MQ=255)                                                                                         cGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAAATTAAAGCCGACGGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCATCACGAAGCCCGTCGTATCGATAGCCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCGGTCGATTGAAGa                                                                                                                                                           <  2:429916/251‑1 (MQ=255)                                                                                            ggTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGACGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAAATTAAAGCCGACAGGCAGGTACGGCACGATGCGGGACTGGAAGCAGGAGGCCTGCATATCATCGGTACCGAGCATCACGAATCCCGTCGGATAGATAACAAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGGTGGTTCTTCCCGTTTCTTCCTGTCGGTGGAAgctgg                                                                                                                                                        >  1:412927/1‑247 (MQ=255)                                                                                                cAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAAATTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCATCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATGCGCTg                                                                                                                                                    <  1:248250/251‑1 (MQ=255)                                                                                                cAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAAATTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCATCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATGCGCTg                                                                                                                                                    >  1:123578/1‑251 (MQ=255)                                                                                                     atTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAAATTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCCTCGGTACCGAGCATCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATGCGCTGATGCg                                                                                                                                               >  2:59481/1‑251 (MQ=255)                                                                                                             cGGTGGTAGATGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAAATTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCATCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCGGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATGCGCTGATGCGTATTTTTg                                                                                                                                       <  2:170303/251‑1 (MQ=255)                                                                                                                              aagAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAAATTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCATCACGAATCCCGTCGTATCGATCACCAGTTGCGCGGTCGTTCTGGTCGGCAGGGGGATGCTGGGTCTTCCCGTTTCTACCTGTCGGTGGAAGATGCGCTGATGAGTATTTTTGCTTccgcccggggatcc                                                                                                                      >  2:237341/2‑244 (MQ=255)                                                                                                                                  aGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAAATTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCATCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGCTGGAAGATGCGCTGATGCGTATTTTTGCTTCCGACCGCGTATCCGGcc                                                                                                                  >  2:47036/1‑250 (MQ=255)                                                                                                                                   gTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAAATTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCATCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATGCGCTGATGCGTATTTTTGCTTCCGACCGAGTATCCGGCa‑t                                                                                                                <  1:52402/251‑1 (MQ=255)                                                                                                                                       ccGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAAATTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCATCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATGCGCTGATGCGTATTTTTGCTTCCGACCGAGTATCCGGCa‑tgatg                                                                                                            <  1:328240/251‑1 (MQ=255)                                                                                                                                                   aTCCGACCGCAGAGCAAATTGAAAAAATTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCATCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATGCGCTGATGCGTATTTTTGCTTCCGACCGAGTATCCGGCA‑TGATGCGTAAACTGGGt                                                                                                <  1:425643/251‑1 (MQ=255)                                                                                                                                                       gACCGCAGAGCAAATTGAAAAAATTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCATCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATGCGCTGATGCGTATTTTTGCTTCCGACCGAGTATCCGGCA‑TGATGCGTAAACTGGGtttg                                                                                             >  1:45598/1‑247 (MQ=255)                                                                                                                                                         ccGCAGAGCAAATTGAAAAAATTAAAGCCGACTGGCAGGTACGTCACGAGGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAACATCACGAATCCCGTCGTATCAATAACCAGTTGCGCCGTCGATCTAGACGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGTAGATGCGCTGATGCAGATTTTTGCTTCCGACCGTATATCCGGCAGTG‑TGCTTAAACTGGGGATGa                                                                                            >  1:20111/1‑249 (MQ=255)                                                                                                                                                          cGCAGAGCAAATTGAAAAAATTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCATCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATGCGCTGATGCGTATTTTTGCTTCCGACCGAGTATCCGGCA‑TGATGCGTAAACTGGGTATGAAGc                                                                                         <  2:162069/251‑1 (MQ=255)                                                                                                                                                                       gAAAAAATTAAAGCCGACGGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCATCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATGCGCTGATGCGTATTTTTGCTTCCGACCGAGTATCCGGCA‑TGATGCGTAAACTGGGTATGAAGCCAGGCGAAGCCAt                                                                            <  2:361158/251‑1 (MQ=255)                                                                                                                                                                             aTTAAAGCCGACTGGCAGGTACGTCACGCTGCGGTACTGGAACCCGGTGGCCAGCATACCATCGGTACCGAGCATCCCGAATCCCGCCGTCTCTATAACCAGTTGCGCGGCCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGCTGGAAGATGCGCCGATGCGTATTTTTGCTTCCGACCCAGTATACGGCA‑TGATGCGTAACCTGGGGGTGAAGCCAGTCGAAGCCATTGAacc                                                                      >  2:428304/1‑250 (MQ=255)                                                                                                                                                                                   gCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCATCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATGCGCTGATGCGTATTTTTGCTTCCGACCGAGTATCCGGCA‑TGATGCGTAAACTGGGTATGAAGCCAGGCGAAGCCATTGAACACCCGTg                                                                <  1:140837/251‑1 (MQ=255)                                                                                                                                                                                     cGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCATCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATGCGCTGATGCGTATTTTTGCTTCCGACCGAGTATCCGGCA‑TGATGCGTAAACTGGGTATGAAGCCAGGCGAAGCCATTGAACACCCGTggg                                                              >  1:13289/1‑251 (MQ=255)                                                                                                                                                                                                 aCGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCATCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATGCGCTGATGCGTATTTTTGCTTCCGACCGAGTATCCGGCA‑TGATGCGTAAACTGGGTATGAAGCCAGGCGAAGCCATTGAACACCCGTGGGTGACTAAAGCGa                                                  <  1:325302/251‑1 (MQ=255)                                                                                                                                                                                                           gCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCATCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATGCGCTGATGCGTATTTTTGCTTCCGACCGAGTATCCGGCA‑TGATGCGTAAACTGGGTATGAAGCCAGGCGAAGCCATTGAACACCCGTGGGTGACTAAAGCGATTGCCAACGc                                        <  2:214255/251‑1 (MQ=255)                                                                                                                                                                                                            cGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCATCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATGCGCTGATGCGTATTTTTGCTTCCGACCGAGTATCCGGCA‑TGATGCGTAAACTGGGTATGAAGCCAGGCGAAGCCATTGAACACCCGTGGGTGACTAAAGCGATTGTCAACGcc                                       <  1:379625/251‑1 (MQ=255)                                                                                                                                                                                                              gTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCATCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATGCGCTGATGCGTATTTTTGCTTCCGACCGAGTATCCGGCA‑TGATGCGTAAACTGGGTATGAAGCCAGGCGAAGCCATTGAACACCCGTGGGTGACTAAAGCGATTGCCAACGCCCa                                     <  1:168635/251‑1 (MQ=255)                                                                                                                                                                                                                     aaGCAGGTGGCCTGCATATCATCGGTACCGAGCATCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATGCGCTGATGCGTATTTTTGCTTCCGACCGAGTATCCGGCA‑TGATGCGTAAACTGGGTATGAAGCCAGGCGAAGCCATTGAACACCCGTGGGTGACTAAAGCGATTGCCAACGCCCAGCGTaaa                              <  1:97978/251‑1 (MQ=255)                                                                                                                                                                                                                             ggCCTGCATATCATCGGTACCGAGCATCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGCTGGAAGATGCGCTGATGCGTATTTTTGCTTCCGACCGAGTATCCGGCA‑TGATGCGTAAACTGGGTATGAAGCCAGGCGAAGCCATTGAACACCCGTGGGTGACTAAAGCGATTGCCAACGCCCAGCGTAAAGTTGaa                        >  1:48379/1‑249 (MQ=255)                                                                                                                                                                                                                                     tatCATCGGTACCGCGCATCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATGCGCTGATGCGTATTTTTGCTTCCGACCGAGTATCCGGCA‑TGATGCGTAAACTGGGTATGAAGCCAGGCGAAGCCATTGAACACCCGTGGGTGACTAAAGCGATTGCCAACGCCCAGCGTAAAGTTGAAAGCCGTAACt              >  2:170287/1‑251 (MQ=255)                                                                                                                                                                                                                                      atcatcGGTACCGAGCATCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATGCGCTGATGCGTATTTTTGCTTCCGACCGAGTATCCGGCA‑TGATGCGTAAACTGGGTATGAAGCCAGGCGAAGCCATTGAACACCCGTGGGTGACTAAAGCGATTGCCAACGCCCAGCGTAAAGTTGAAAGCCGTAACtt             >  2:92447/1‑251 (MQ=255)                                                                                                                                                                                                                                           cGGTACCGAGCATCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATGCGCTGATGCGTATTTTTGCTTCCGACCGAGTATCCGGCA‑TGATGCGTAAACTGGGTATGAAGCCAGGCGAAGCCATTGAACACCCGTGGGTGACTAAAGCGATTGCCAACGCCCAGCGTAAAGTTGAAAGCCGTAACTTCGAc         <  2:167317/250‑1 (MQ=255)                                                                                                                                                                                                                                              tACCGAGCATCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATGCGCTGATGCGTATTTTTGCTTCCGACCGAGTATCCGGCA‑TGATGCGTAAACTGGGTATGAAGCCAGGCGAAGCCATTGAACACCCGTGGGTGACTAAAGCGATTGCCAACGCCCAGCGTAAAGTTGAAAGCCGTAACTTCGACATTc     >  1:359152/1‑251 (MQ=255)                                                                                                                                                                                                                                                  gAGCATCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATGCGCTGATGCGTATTTTTGCTTCCGACCGAGTATCCGGCA‑TGATGCGTAAACTGGGTATGAAGCCAGGCGAAGCCATTGAACACCCGTGGGTGACTAAAGCGATTGCCAACGCCCAGCGTAAAGTTGAAAGCCGTAACTTCGCCATTCGTa  >  2:128716/1‑250 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       GAACGCCAAATTCCACGCCAACGAAGCGGCGATTGTTGCTCAGGCAGGTTATCCGGCTGCGGTGACTATCGCGACCAATATGGCGGGTCGTGGTACAGATATTGTGCTCGGTGGTAGCTGGCAGGCAGAAGTTGCCGCGCTGGAAAATCCGACCGCAGAGCAAATTGAAAAAATTAAAGCCGACTGGCAGGTACGTCACGATGCGGTACTGGAAGCAGGTGGCCTGCATATCATCGGTACCGAGCGTCACGAATCCCGTCGTATCGATAACCAGTTGCGCGGTCGTTCTGGTCGTCAGGGGGATGCTGGTTCTTCCCGTTTCTACCTGTCGATGGAAGATGCGCTGATGCGTATTTTTGCTTCCGACCGAGTATCCGGCA‑TGATGCGTAAACTGGGTATGAAGCCAGGCGAAGCCATTGAACACCCGTGGGTGACTAAAGCGATTGCCAACGCCCAGCGTAAAGTTGAAAGCCGTAACTTCGACATTCGTA  >  NC_000913/109715‑110205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑