Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,604,3500CT34.8% 19.4 / 10.5 23S64F (TCT→TTT) bglJDNA‑binding transcriptional regulator BglJ
Reads supporting (aligned to +/- strand):  ref base C (6/9);  new base T (8/0);  total (14/9)
Fisher's exact test for biased strand distribution p-value = 7.17e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATTAAGTTTCCGCGTACCCGGCGTTTAG‑TTATTGCGGATGATGATAT‑TGAAGCTCGGCTG‑ATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGCTGGAGA‑TTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCG  >  NC_000913/4604104‑4604578
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                         
aGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATATCGTTTGCGGCGGGCATTttttctttttcttcc                                                                                                                                                                                                                                       >  1:240952/1‑248 (MQ=255)
 gcgcgcAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCa                                                                                                                                                                                                                                      <  1:266813/251‑1 (MQ=255)
                                 tCTGTTGATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCGCGTTTGCGGCGGTCATTTTTTCTTGTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCtt                                                                                                                                                                                                        <  2:388113/249‑1 (MQ=255)
                                              aTGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTgg                                                                                                                                                                                         <  1:97864/251‑1 (MQ=255)
                                                                                     tGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTGCCCTGAGAAGGGAGCGCCGAGAGGGGATATCTTGCCTGACTGAACTGGCG‑ATTAAGTTTCCGCGTACCCGGCGTTTAG‑TTTTTGCgg                                                                                                                                                >  1:418922/1‑251 (MQ=255)
                                                                                        cGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTGCCCAGAGGAGATAGAGGAGAGAGGGATTGTCTTGCCTGAATGAGATGGCG‑GTTAAGTTTGCGCGAACCCAGCGTTTTG‑TTATTTCgggtg                                                                                                                                             >  2:247913/1‑248 (MQ=255)
                                                                                          cACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTGCCCTGAGGAGGGAGGGCCGAGGGGGGTTATTTTTCCTGACTTAACTGGGG‑GTTTAGTTTCCCCGTTACCGGCggtttgttttttcgggtggt                                                                                                                                            >  2:298263/1‑232 (MQ=255)
                                                                                           aCTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTCTTTTTCTGCCATGAGAAGGGAGCGCAGTGAGGGGTTTTCTTGCCTGACTTAACTGGCG‑CTTAAGTTTCCGCGTACCCAGCGTTTTG‑TTATTGCgggagttg                                                                                                                                          >  1:159842/1‑245 (MQ=255)
                                                                                                      aaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCCTGAGAAAGGAGCGCAGAGAGGGGTTTTCTTTCCTGACTGCACTGGGG‑ATTAAGTTTCCGCGTACCCGGCGGTTTG‑TTCTTTCGGGTGATGAGAT‑TTAAGCt                                                                                                                              >  1:260013/1‑251 (MQ=255)
                                                                                                          cGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTCTTTTTCTTCCCTTTGGAGTGAGCGCAGAGAGGGGTTATCTTTCCTGACTGAACTGGCG‑AATAAGTTTTCCCGTCCCCGGCGTTTAGGTCATT‑TGGATGATGATATGTGA‑GCTCGGc                                                                                                                          >  1:89626/1‑251 (MQ=255)
                                                                                                           gTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGTGAGCGCAGAGAGGGGTTATTTTGCCTGACTGAACTGGCG‑GTTAAGTTTCCCCGTACCCGGCGGTTTG‑TTATTGCGGATGATGGTAT‑TGAAGCTCgggt                                                                                                                         >  1:384179/1‑249 (MQ=255)
                                                                                                                            tATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGAATTTTTTCTTTTTCTGCCATGCGGAGGGAGCGCAGAGGGGGGTGTTCTTGCCTTGCTGAACTGGCG‑GTTTAGTTTTCGCGGACCCGGGGGTTAG‑TTTTTTCGGATGATGGTAT‑TTAAACTCGGCTggataggtctttgtcgc                                                                                                        >  2:314230/1‑235 (MQ=255)
                                                                                                                                 agagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGCGAAGTGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAAATGGGGGATTA‑GTTTCCGCGGACCCGGCGTTTTT‑GTATTGTGGGTGATGATAT‑TGATGGTCGGCTG‑GTTGGTTTAtttttgccctcc                                                                                                  >  2:375579/1‑241 (MQ=255)
                                                                                                                                        aTTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTTCTTTTTTCTTTTTTTTCCATGGGGAGGGACCGCAGAGGGGGGTTTTCTTTCCTGGCTCACCTGGGG‑ATTAAGTTTCCACGTACCCGGCGGTTCG‑TTATTTCGGGTGATGGGAT‑TTAAACTCGGCTG‑GTTGTTTTTTTGTCGCCATCACCGCTgg                                                                                           >  1:207980/1‑251 (MQ=255)
                                                                                                                                             gAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATTAAGTTTCCGCGTACCCGGCGTTTAG‑TTATTGCGGATGATGATAT‑TGAAGCTCGGCTG‑ATTGGTTCATTGTCGCCATCACCGCTGGACGgt                                                                                      <  2:384179/251‑1 (MQ=255)
                                                                                                                                                    tGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATTAAGTTTCCGCGTACCCGGCGTTTAG‑TTATTGCGGATGATGATAT‑TGAAGCTCGGCTG‑ATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTa                                                                                <  2:137259/250‑1 (MQ=255)
                                                                                                                                                                       cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATTAAGTTTCCGCGTACCCGGCGTTTAG‑TTATTGCGGATGATGATAT‑TGAAGCTCGGCTG‑ATTGGTTCATTGTCGCCATCACCGCTGGAc                                                                                         <  2:176284/222‑1 (MQ=255)
                                                                                                                                                                               cTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTGCCATGAGAAGGGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATTAAGTTTCCGCGTACCCGGCGTTTAG‑TTATTGCGGGGGATGATAT‑TGAAGCTCGGCTG‑ATTGGGTCATTGTCGCCATCACCGCTGGGCGGGGGATTAAGTAAAGCg                                                                       >  1:114584/1‑232 (MQ=255)
                                                                                                                                                                               cTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATTAAGTTTCCGCGTACCCGGCGTTTAG‑TTATTGCGGATGATGATAT‑TGAAGCTCGGCTG‑ATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCg                                                                       <  2:114584/232‑1 (MQ=255)
                                                                                                                                                                                 ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTCTTTTTTTGCCCTGAGGAGTGGGAGCAGAGAGGGATTATCTTTTCTGCCTGAACTGGCG‑GTTAAGTTTTCGCGTACCCGGCGTTTAG‑TTATTTCGGGTGGTGGTAT‑TGAAGCTCGGCTT‑GTTGGTTTATTTTTGCCCTCACCCCTGGGCGGGGGATTTAATAAAGAGGCAACGCTGGGGA‑TTTTtcct                                                 >  2:275124/1‑249 (MQ=255)
                                                                                                                                                                                         cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTTATTTTTTCTTTTTCTGCCATGAGAAGGGAGCGCCGGGAGGGGTTATACTGCCTGACTGGACTGGGG‑GATAAGGTTCCGCCTACCCGGCGGTTTG‑TTAATGCGGATGGTGATAA‑TGAAGCTCGGCTGGTTTGGTTGTTTG‑CGCCAACACCGCTGGACGGGGGAATAAGCAAAGCGGCAACTCTGGGGAATTTTTC‑TCAGGaaac                                         >  1:395267/1‑249 (MQ=255)
                                                                                                                                                                                                                  cgcgGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATTAAGTTTCCGCGTACCCGGCGTTTAG‑TTATTGCGGATGATGATAT‑TGAAGCTCGGCTG‑ATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGCTGGAGA‑TTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACg                 <  1:275124/250‑1 (MQ=255)
                                                                                                                                                                                                                         ataggttgaggcgtTCATGTTTGCTTTTTCTGTCAGGAGAAGTGAGTGCAGAGAGTGAGTATTGTGCCTCACTGAACTGGTG‑TTTAAGTTTCCCTGTACCCGGCGTTTAG‑GTATTGCGGATGCTGATAT‑TGAAGCTCGGCTG‑ATTGGTCCATTGTCGCCATTACCGCTGGACGGTGTATTAAGTAAAGCGTCATCGCTGGAGA‑TTGTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGcg          <  1:364543/241‑1 (MQ=255)
                                                                                                                                                                                                                                 cggcggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATTTTGCCTGACTGAACTGGCG‑CTTAAGTTTCCGCGTACCCGGCGTTTAG‑TTATTGCGGATGATGATAT‑TGAAGCTCGGCTG‑GTTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAAAGCTGGAGA‑TTTTTCATCCGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGcgaccgaccg  >  2:16203/1‑250 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                         
AGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATTAAGTTTCCGCGTACCCGGCGTTTAG‑TTATTGCGGATGATGATAT‑TGAAGCTCGGCTG‑ATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGCTGGAGA‑TTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCG  >  NC_000913/4604104‑4604578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑