| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 651,267 | 0 | A | T | 22.9% | 136.3 / 16.6 | 61 | L197* (TTA→TAA) | citC | citrate lyase synthetase |
| Reads supporting (aligned to +/- strand): ref base A (7/40); new base T (14/0); total (21/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.17e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGCCAGTAGCGCATATCCTGGTTGTACTGGGCGGTAACGCGACAAAAGGGTTCAGTACCGACAAAGCGGTGAGTTACACCCAGCGCGGGAGCGAGGTACTGACGGAAAATCTTCAGATCAATTTCGGTGTAACAATGGTTAATGACGCTCTGTTCTTTAATGAAGTAGCAAGGGAACGTAGCGCGGGAGATGATGTATTCGGAGCCACGATGCACAGTCAGGCGTGGAATATCGGCGGTG‑CC‑TTTTAACACCAAATCCAGCCGGTCTTCATA‑GGGGAAGCGTGAAGAATCTTCTTTGACTAAAAACA‑GATGCAACCAGTCGCACTGTGCCGCAGCCTGTTGAATCAGATAACGGTGACCATTCGTAAAGGGATTGGCGTTCATCACAATGCAGCCAATCTTGTTCCCTGGATGACGAAATTTTTTCAGCGATTCGGCATAGCGTTTCAGTCGCGTGGCGCTGTTTTCCATCAGCACCATCACGCCGGGTACGCTGG > NC_000913/651020‑651516 | cagccagTAGCGCATATCCTGGTGGTACTGGGCGGGAACGCGACAAAAGGGTTCAGTACCGACAAAGCGGTGAGGTACACCCAGCGCGGGAGCGAGGTACTGACGGAAAAGCTTCAGATCAATTGCGGTGTAACAATGGTTAATGACGCTCTGTTCTTTAATGAAGTAGCAAGGGAACGTAGCGCGGGAGATGATGTATTCGGAGCCACGATGCACAGTCAGGCGTTGAATATCGGCGGTG‑CC‑TTTTAaca < 2:102362/251‑1 (MQ=255) agccagTAGCGCATATCCTGGTTGTACTGGTCGGTAACGCGACAAAAGGGTGCAGTACCGACAAAGCGGTGAGTTACACCCAGCGCGGGAGCGAGGTACTGACGGAAAATCTTCAGATCAATTTCGGTGTAACAATGGTTAATGACGCTCTGTTCTTTAATGAAGTAGCAAGGGAACGTAGCGCGGGAGATGATGTATTCGGAGCCACGATGCACAGTCAGGCGTGGAATATCGGCGGTG‑CC‑TTTTAacac < 1:355437/251‑1 (MQ=255) agTAGCGCATATCCTGGTTGTACTGGGCGGTAACGCGACAAAAGGGTTCAGTACCGACAAAGCGGTGAGTTACACCCAGCGCGGGAGCGAGGTACTGACGGAAAATCTTCAGATCAATTTCGGTGTAACAATGGTTAATGACGCTCTGTTCTTTAATGAAGTAGCAAGGGAACGTAGCGCGGGAGATGATGTATTCGGAGCCACGATGCACAGTCAGGCGTGGAATATCGGCGGTG‑CC‑TTTTTACAccca > 2:7777/1‑248 (MQ=255) agTAGCGCATATCCTGGTTGTACTGGGCGGTAACGCGACAAAAGGGTTCAGTACCGACAAAGCGGTGAGTTACACCCAGCGCGGGAGCGAGGTACTGACGGAAAATCTTCAGATCAATTTCGGTGTAACAATGGTTAATGACGCTCTGTTCTTTAATGAAGTAGCAAGGGAACGTAGCGCGGGAGATGATGTATTCGGAGCCACGATGCACAGTCAGGCGTGGAATATCGGCGGTG‑CC‑TTTTAACACCaaa < 1:420590/251‑1 (MQ=255) cATATCCTGGTTGTACTGGGCGGTAACGCGACAAAAGGGTTCAGTACCGACAAAGCGGTGAGTTACACCCAGCGCGGGAGCGAGGTACTGACGGAAAATCTTCAGATCAATTTCGGTGTAACAATGGTTAATGACGCTCTGTTCTTTAATGAAGTAGCAAGGGAACGTAGCGCGGGAGATGATGTATTCGGAGCCACGATGCACAGTCAGGCGTGGAATATCGGCGGTG‑CC‑TTTTAACACCAAATCCAGcc < 2:386893/251‑1 (MQ=255) gggCGGTAACGCGACAAAAGGGTTCAGTACCGACAAAGCGGTGAGTTACACCCAGCGCGGGAGCGAGGTACTGACGGAAAATCTTCAGATCAATTTCGGTGTAACAATGGTTAATGACGCTCTGTTCTTTAATGAAGTATCAAGGGATCGTAGCGCGGGATATGATGTATTCGGATCCACGATGCACAGTCAGGCGTGGAATATCGGCGGTG‑CC‑TTTTAACACCAAATCCAGCCGGTCTTCATA‑GGGGAAg < 1:295269/251‑1 (MQ=255) gCGGCAACGCGACAAAAGGGTTCAGTACCGACAAAGCGGTGAGTTACACCCAGCGCGGGAGCGAGGTACTGACGGAAAATCTTCAGATCAATTTCGGTGTAACAATGGTTAATGACGCTCTGTTCTTTAATGAAGTAGCAAGGGTACGTAGCGCGGGAGATGATGTATTCGGAGCCACGATGCCAAGTCAGGCGTGGAATATCGGCGGTG‑CC‑TTTTTACACCAAATACAACCGTTCTTCATAAGGGGaacg > 2:357749/1‑249 (MQ=255) cGGTAACGCGCCAAAAGGGTTCAGTACCGACAAAGCGGTGAGTTACACCCAGCGCGGGAGCGAGGTACTGACGGAAAATCTTCAGATCAATTTCGGTGTAACAATGGTTAATGACGCTCTGTTCTTTAATGAAGTAGCAAGGGAACGTAGCGCGGGAGATGATGTATTCGGAGCCACGATGCACAGTCAGGCGTGGAATATCGGCGGTG‑CC‑TTTTTACACCCAAACCAGCCGGTCTTCTTA‑GGGGAAGCgg > 2:77733/1‑250 (MQ=255) cGGTAACGCGACAAAAGGGTTCAGTACCGACAAAGCGGTGAGTTACACCCAGCGCGGGAGCGAGGTACTGACGGAAAATCTTCAGATCAATTTCGGTGTAACAATGGTTAATGACGCTCTGTTCTTTAATGAAGTAGCAAGGGAACGTAGCGCGGTAGATGATGTATTCGGAGCCACGATGCACAGTCAGGCGTGGAATATCGGCGGTG‑CC‑TTTTAACACCAAAGCCAGCCGGTCTTCATA‑GGGGAAGCGt < 2:287/251‑1 (MQ=255) ggagcgcgACAAAAGGGTTCAGGACCGACATAGCGGTGAGTTACACCCAGCGCGGGAGCGAGGTACTGACGGAAAATCTTCAGATCAATTTCGGTGTAACAATGGTTAATTACGCTCTGTTCTTTAATGAAGTAGCAAGGGAACGTAGCCCGGGAGATGATGTATTCGGAGCCACGATGCACTGTCAGGCGTGGAATAGCGGGGGTG‑CC‑TTTTAACACCAAATCCAGCCGGTCTTCATA‑GGGGAAGCGTga < 1:222833/247‑1 (MQ=255) gTAACGCGACAAAAGGGTTCAGTACCGACAAAGCGGTGAGTTACACCCAGCGCGGGAGCGAGGTACTGACGGAAAATCTTCAGATCAATTTCGGTGTAACAATGGTTAATGACGCTCTGTTCTTTAATGAAGTAGCAAGGGAACGTAGCGCGGGAGATGATGTATTCGGAGCCACGATGCACAGTCAGGCGTGGAATATCGGCGGTG‑CC‑TTTTTACACCAAATCCACCCGGGTTTCATA‑GGGGGAGCGTgg > 1:222396/1‑250 (MQ=255) cgcgAAAAAAGGGTTCAGTACCGACAATGCGGTGAGTTACACACAGCGCGGGAGCGAGGTACTGACGGAAAATCTTCAGATCAATTTCGGTGTAACAATGGTTAATGACGCTCTGTTCTTTAATGAAGTAGCAAGGGAACGTAGCGCGGGAGATGCTGTATTCGGAGCCACGATGCACAGTCAGGCGTGGAATATCGGCGGTG‑CC‑TTTTTATACCACATCCAACCGGGCTTCATA‑gggggagggggtcga > 2:406755/1‑239 (MQ=255) gcgACAAAAGGGTTCAGTACCGACAAAGCGGTGAGTTACACCCAGCGCGGGAGCGAGGTACTGACGGAAAATCTTCAGATCAATTTCGGTGTAACAATGGTTAATGACGCTCTGTTCTTTAATGAAGTAGCAAGGGAACGTAGCGCGGGAGATGATGTATTCGGAGCCACGATGCACAGTCAGGCGTGGAATATCGGCGGTG‑CC‑TTTTAACACCAAATCCAGCCGGTCTTCATA‑GGGGAAGCGTGAAGAAt < 1:7777/251‑1 (MQ=255) aCAAAAGGGTTCAGTACCGACAAAGCGGTGAGTTACACCCAGCGCGGGAGCGAGGTACTGACGGAAAATCTTCAGATCAATTTCGGTGTAACAATGGTTAATGACGCTCTGTTCTTTAATGAAGTAGCAAGGGAACGTAGCGCGGGAGATGATGTATTCGGAGCCACGGTGCACAGTCAGGCGTGGAATATCGGCGGTG‑CC‑ATTTAACACCAAATCCAGCCGGTCTTCATA‑GGGGAAGCGTGAAGAAtctt < 2:316088/251‑1 (MQ=255) aCAAAAGGGTGCAGTACCGACAAAGCGGTGAGTTACACCCAGCGCGGGAGCGAGGTACTGACGGAAAATCTTCAGATCAATTTCGGTGTAACAATGGTTAATGACGCTCTGTTCTTTAATGAAGTAGCAAGGGAACGTAGCGCGGGAGATGATGTATTCGGAGCCACGATGCACAGTCAGGCGTGGAATATCGGCGGTG‑CC‑TTTTAACACCAAATCCAGCCGGTCTTCATA‑GGGGAAGCGTGAAGAAtctt < 2:332570/251‑1 (MQ=255) aGGGGTCAGTACCGACAAAGCGGTTAGTTACACCCAGCGCGGTAGCGAGGTACTGACGTAAAATCTTCAGATCAATTTCGGTGTAACAATGGTTAATGACGCTCTGTTCTTTAATGAAGTAGCAAGGGAACGTAGCGCGGGAGATGATTTATTCGGAGCCACGATGCACAGTCAGTCGTGGAATATCGGCGGTG‑CC‑TTTTAACACCAAATCCAGCCGGTCTTCATA‑GGGGAAGCGTGAAGAATCTTCTTTg < 2:238137/251‑1 (MQ=255) cAGTACCGACAAAGCGGTGAGTTACACCTAGCGCGGGAGCGAGGTACTGACGGAAAATCTTCAGATCAATTTCGGTGTAACAATGGTTAATGACGCTCTGTTCTTTAATGAAGTAGCAAGGGAACGTAGCGCGGGAGATGATGTATTCGGAGCCACGATGCACAGTCAGGCGTGGAATATCGGCGGTG‑AC‑TTTTTACACCAAATCCAGCCGGGGTTCATA‑GGGGAAGAGGGAAGAATCTTCTTTTACTaa > 1:269635/1‑250 (MQ=255) cAGTACCGACAAAGCGGTGAGTTACACCCAGCGCGGGAGCGAGGCCCTGCCGGAAAATCTTCAGATCAATTTCGGTGTAAAAATGGTTAATGACGCTCTGTTCTTTAATGACGTAGCAAGGGAACGTAGCGCGGGAGATGATGTATTCGGAGCCACGATGCACAGTCAGGCGTGGAATATCGGCGGTA‑CC‑CTTTAACACCAAATCCCGCCGGTCTTCCTC‑AGGGAGGGGTGCAGAAACTTCTTTgcgcaa > 1:20561/1‑245 (MQ=255) gACAAAGCGGTGAGTTACACCCAGCGCGGGAGCGAGGTACTGACGGAAAATCTTCAGATCAATTTCGGTGTAACAATGGTTAATGACGCTCTGTTCTTTAATGAAGTAGCAAGGGAACGTAGCGCGGGAGATGATGTATTCGGAGCCACGATGCACAGTCAGGCGTGGAATATCGGCGGTG‑CCCTTTTAA‑ACCACAACCCCCCGGCCCTCATA‑TGGGGAGCGGGGAGAATCTTCTTTTATTAAAAACaaat > 2:80402/1‑248 (MQ=255) aaaGCGGTGAGTTACACCCAGCGCGGGAGCGAGGTACTGACGGAAAATCTTCAGATCAATTTCGGTGTAACAATGGTTAATGACGCTCTGTTCTTTAATGAAGTAGCAAGGGAACGTAGCGCGGGAGATGATGTATTCGGAGCCACGATGCACAGTCAGGCGTGGAATATCGGCGGTG‑CC‑TTTTTACACCAAAACCAGCCGGTCTTCATA‑GGGGGAGCGGGAAGAAACTTCTTTTACTAAAAACA‑GATGc > 1:339793/1‑250 (MQ=255) gAGTTACACCCAGCGCGGGAGCGAGGTACTGACGGAAAATCTTCAGATCAATTTCGGTGTAACAATGGTTAATGACGCTCTGTTCTTTAATGAAGTAGCAAGGGAACGTAGCGCGGGAGATGATGTATTCGGAGCCACGATGCACAGTCAGGCGTGGAATATCGGCGGTG‑CC‑TTTTAACACCAAATCCAGCCGGTCTTCATA‑GGGGAAGCGTGAAGAATCTTCTTTGACGAAAAACA‑GATGCATCCAGTc < 2:84874/250‑1 (MQ=255) ttACACCCAGCGCGGGAGCGAGGTACTGACGGAAAATCTTCAGATCAATTTCGGTGTAACAATGGTTAATGACGCTCTGTTCTTTAATGAAGTAGCAAGGGAACGTAGCGCGGGAGATGATGTATTCGGAGCCACGATGCACAGTCAGGCGTGGAATATCGGCGGTG‑CC‑TTTTTACACCAAAACCACGCGGGCTTTATA‑AGGGAAGCGTGAAGAATCTTCTTTTACTAAAAACA‑AATGCAACCaagcgccc > 1:190746/1‑244 (MQ=255) ccAGCGCGGGAGCGAGGTACTGACGGAAAATCTTCAGATCAATTTCGGTGTAACAATGGTTAATGACGCTCTGTTCTTTAATGAAGTAGCAAGGGAACGTAGCGCGGGAGATGATGTATTCGGAGCCACGATGCACAGTCAGGCGTGGAATATCGGCGGTG‑CC‑TTTTAACACCAAATCCAGCCGGTCTTCATA‑GGGGAAGCGTGAAGAATCTTCTTTGACTAAAAACA‑GATGCAACCAGTCGCACTGTGcc < 2:394355/251‑1 (MQ=255) ggAGCGAGGTACTGACGGAAAATCTTCAGATCAATTTCGGTGTAACAATGGTTAATGACGCTCTGTTCTTTAATGAAGTAGCAAGGGAACGTAGCGCGGGAGATGATGTATTCGGAGCCACGATGCACAGTCAGGCGTGGAATATCGGCGGTG‑CC‑TTTTAACACCAAATCCAGCCGGTCTTCATA‑GGGGAAGCGTGAAGAATCTTCTTTGACTAAAAACA‑GATGCAACCAGTCGCACTGTGCCGCAGCCTg < 1:424717/251‑1 (MQ=255) gAGGTACTGACGGAAAATCCTCAGATCAATTTCGGTGTAACAATGGTTAATGACGCTCTGTTCGTTAATGAAGTAGCAAGGGAACGTTGCGCGGGAGATGATGTATTCGGAGCCACGAGGCACAGTCAGGCGTGGAATATCGGCGGTG‑CC‑TTTTAACACCAAATCCAGCCGGTCTTCATA‑GGGGAAGCGTGAAGAATCTTCTTTGACTAAAAACA‑GATGCACCCAGGCGCACTGTGCCGCAGCCTTTTGaa < 2:431513/251‑1 (MQ=255) aCGGAAAATCTTCAGATCAATTTCGGTGTAACAATGGTTAATGACGCTCTGTTCTTTAATGAAGTAGCAAGGGAACGTAGCGCGGGAGATGATGTATTCGGAGCCACGATGCACAGTCAGGCGTGGAATATCGGCGGTG‑CC‑TTTTAACACCAAATCCAGCCGGTCTTCATA‑GGGGAAGCGTGAAGAATCTTCTTTGACTAAAAACA‑GATGCAACCAGTCGCACTGTGCCGCAGCCTGTTGAATCAGATAAc < 1:181010/251‑1 (MQ=255) aaTCTTCAGATCAATTTAGGTGTAACAATGGTTAATGACGCTCTGTTCGTTAATGAAGTAGCAAGGTAACGTAGCGCGGGAGATGAGGTAGTCGGAGCCACGATGCCCAGTCAGGCGTGGAATATCGGCGGTG‑CC‑TTTTAACACCAAATCCAGCCGGTCTTCATA‑GGGGAAGCGTGAAGAATCTTCTTTGACGAAAAACA‑GATGCAACCAGTCGCACTGTGCCGGAGCCTGTTGAATCAGATAAGGGTGAc < 2:276125/251‑1 (MQ=255) gATCAATTTCGGTGTAACAATGGTTAATGACGCTCTGTTCTTTAATGAAGTAGCAAGGGAACGTAGCGCGGGAGATGATGTATTCGGAGCCACGATGCACAGTCAGGCGTGGAATATCGGCGGTG‑CC‑TTTTAACACCAAATCCAGCCGGTCTTCATA‑GGGGAAGCGTGAAGAATCTTCTTTGACTAAAAACA‑GATGCAACCAGTCGCACTGTGCCGCAGCCTGTTGAAGCAGATAACGGTGACCATTCGTa < 2:401489/251‑1 (MQ=255) cGGTGTAACAATGGTTAATGACGCTCTGTTCTTTAATGAAGTAGCAAGGGAACGTAGCGCGGGAGATGATGTATTCGGAGCCACGATGCACAGTCAGGCGTGGAATATCGGCGGTG‑CC‑TTTTAACACCAAATCCAGCCGGTCTTCATA‑GGGGAAGCGTGAAGAATCTTCTTTGACTAAAAACA‑GATGCAACCAGTCGCACTGTGCCGCAGCCTGTTGAATCAGATAACGGTGACCATTCGTAAAGGGAtt < 2:339793/250‑1 (MQ=255) aaCAATGGTTAATGACGCTCTGTTCTTTAATGAAGTAGCAAGGGAACGTAGCGCGGGAGATGATGTATTCGGAGCCACGATGCACAGTCAGGCGTGGAATATCGGCGGTG‑CC‑TTTTAACACCAAATCCAGCCGGTCTTCATA‑GGGGAAGCGTGAAGAATCTTCTTTGACTAAAAACA‑GATGCAACCAGTCGCACTGTGCCGCAGCCTGTTGAATCAGATAACGGTGACCATTCGTAAAGGGATTGGCGTtc < 2:426562/251‑1 (MQ=255) aCAATGGTTAATGACGCTCTGTTCTGTAATGAAGTAGCAAGGGAACGTAGCGCGGGAGATAATGTATTCGGAGCCACGATGCGCAGTCAGGCGTGGAATATCGGCGGTG‑CC‑TTTTAACACCAAATCCAGCCGGTCTTCATA‑GGGGAAGCGTGAAGAATCTTCTTTGACTAAAAACA‑GATGCAACCAGTCGCACTGTGCCGCAGCCTGTTGAATCAGATAACGGTGACCATTCGTAAAGGGATTGGCGTtca < 2:21188/251‑1 (MQ=255) cAATGGTTAATGACGCTCTGTTCTTTAATGAAGTAGCAAGGGAACGTAGCGCGGGAGATGATGTATTCGGAGCCACGATGCACAGTCAGGCGTGGAATATCGGCGGTG‑CC‑TTTTAACACCCAATCCAGCCGGTCTTCATA‑GGGGAAGCGTGAAGAATCTTCTTTGACTAAAAACA‑GATGCAACCAGTCGCCCTGTGCCGCAGCCTGTTGAATCAGAt > 1:351993/1‑217 (MQ=255) cAATGGTTAATGACGCTCTGTTCTTTAATGAAGTAGCAAGGGAACGTAGCGCGGGAGATGATGTATTCGGAGCCACGATGCACAGTCAGGCGTGGAATATCGGCGGTG‑CC‑TTTTAACACCAAATCCAGCCGGTCTTCATA‑GGGGAAGCGTGAAGAATCTTCTTTGACTAAAAACA‑GATGCAACCAGTCGCACTGTGCCGCAGCCTGTTGAATCAGAt < 2:351993/217‑1 (MQ=255) tGGTTAATGACGCTCTGTTCTTTAATGAAGTAGCAAGGGAACGTAGCGCGGGAGATGATGTATTCGGAGCCACGATGCACAGTCAGGCGTGGAATATCGGCGGTG‑CC‑TTTTTAAACCACATCCAGCCGGGCTTCATT‑GGGGAAGCGGGAAGAATATTCTTTTTATAAAAAAA‑GATGCCACCAGGCGCACCGCGCCGCCGCCTCTTGAATCAGAAAAAGGGGGCCATTCTTAAAGGGATTGGCGTtcattac > 2:26053/1‑248 (MQ=255) ggTTAATGACGCTCTGTTCTTTAATGAAGTAGCAAGGGAACGTAGCGCGGGAGATGATGTATTCGGAGCCACGATGCACAGTCAGGCGTGGAATATCGGCGGTG‑CC‑TTTTTACCCCAAATCCAGCCGGGCTTCATT‑TGGGGAGCGTGGAGAATCTTCTTTGACTAAAAAAA‑GATGCAACCAGTCGCGCTGTGCCGCAGCCTGTTGATTCAAATAACGGTGGACATTCGTAAAGGGGTTGGCGTttatccc > 2:135294/1‑248 (MQ=255) tAATGACGCTCTGTTCTTTAATGAAGTAGCAAGGGAACGTAGCGCGGGAGATGATGTATTCGGAGCCACGATGCACAGTCAGGCGTGGAATATCGGCGGTGACC‑TTTTA‑CACCCAAACCAGCCGGTCTTCATA‑GGGGGAGCGGGAAGAATCTTCTTTGACTAAAAAAA‑AAAGCAACCAGAAGCACTGTGCCGCCGCCTGGTGAATCAAATAAAGGGGACCATTCGTAAAGGGGTTGGCggttatcccaatt > 1:194077/1‑239 (MQ=255) gACGCTCTGTTCTTTAATGAAGTAGCAAGGGAACGTAGCGCGGGAGATGATGTATTCGGAGCCACGATGCACAGTCAGGCGTGGAATATCGGCGGTG‑CC‑TTTTAACACCAAATCCAGCCGGTCTTCATA‑GGGGAAGCGTGAAGAATCTTCTTTGACTAAAAACA‑GATGCAACCAGTCGCACTGTGCCGCAGCCTGTTGAATCAGATAACGGTGACCATTCGTAAAGGGATTGGCGTTCATCACAATGCAg < 1:83890/250‑1 (MQ=255) aCGCTCTGTTCTTTAATGAAGTAGCAAGGGAACGTAGCGCGGGAGATGATGTATTCGGAGCCACGATGCCCAGTCAGGCGTTGAATATCGGCGGTG‑CC‑TTTTAACACCAAATCCAGCCGGTCTTCATA‑GGGGAAGCGTGAAGAATCTTCTTTGACTAAAAACA‑GATGCAACCAGTCGCACTGTGCCGCAGCCTGTTGAATCAGATAACGGTGACCATTCGTAAAGGGATTGGCGTTCATCACAATGCAGcc < 1:287607/251‑1 (MQ=255) gTTCTTTAATGAAGTAGCAAGGGAACGTAGCGCGGGAGATGATGTATTCGGAGCCACGATGCACAGTCAGGCGTGGAATATCGGCGGTG‑CC‑TTTTAACACCAAATCCAGCCGGTCTTCATA‑GGGGAAGCGTGAAGAATCTTCTTTGACTAAAAACA‑GATGCAACCAGTCGCACTGTGCCGCAGCCTGTTGAATCAGAGAACGTGGACCATTCGTAAAGGGATTGGCGGTCATCACAATGCAGCCAATCTTg < 2:376831/251‑1 (MQ=255) ttAATGAAGTAGCAAGGGAACGTAGCGCGGGAGATGATGTATTCGGAGCCACGATGCACAGTCAGGCGTGGAATATCGGCGGTG‑CC‑TTTTAACACCAAATCCAGCCGGTCTTCATA‑GGGGAAGCGTGAAGAATCTTCTTTGACTAAAAACA‑GATGCAACCAGTCGCACTGTGCCGCAGCCTGTTGAATCAGATAACGGTGACCATTCGTAAAGGGATTGGCGTTCATCACAATGCAGCCAATCTTGTTcc < 1:111110/250‑1 (MQ=255) ttAATGAAGTAGCAAGGGAACGTAGCGCGGGAGATGATGTATTCGGAGCCACGATGCACAGTCAGGCGTGGAATATCGGCGGTG‑CC‑TTTTAACACCAAATCCAGCCGGTCTTCATA‑GGGGAAGCGTGAAGAATCTTCTTTGACTAAAAACA‑GATGCAACCAGTCGCACTGTGCCGCAGCCTGTTGAATCAGATAACGGTGACCATTCGTAAAGGGATTGGCGTTCATCACAATGCAGCCAATCTTGTTcc < 1:29625/250‑1 (MQ=255) aaTGAAGTAGCAAGGGAACGTAGCGCGGGAGATGATGTATTCGGAGCCACGATGCACAGTCAGGCGTGGAATATCGGCGGTG‑CC‑TTTTAACACCAAATCCAGCCGGTCTTCATA‑GGGGAAGCGTGAAGAATCTTCTTTGACTAAAAACA‑GATGCAACCAGTCGCACTGTGCCGCAGCCTGTTGAATCAGATAACGGTGACCATTCGTAAAGGGATTGGCGTTCATCACAATGCAGCCAATCTTGTTCCCTg < 1:248681/251‑1 (MQ=255) aaGTAGCAAGGGAACGTAGCGCGGGAGATGATGTATTCGGAGCCACGATGCACAGTCAGGCGTGGAATATCGGCGGTG‑CC‑TTTTAACACCAAATCCAGCCGGTCTTCATA‑GGGGAAGCGTGAAGAATCTTCTTTGACTAAAAACA‑GATGCAACCAGTCGCACTGTGCCGCAGCCTGTTGAATCAGATAACGGTGACCATTCGTAAAGGGATTGGCGTTCATCACAATGCAGCCAATCTTTTTCCCTGGATg < 2:393687/251‑1 (MQ=255) aggaGCAAGGTAACGTAGCGCGGGAGATGATGTATTCGGAGCCACGATGCACAGTCAGGCGTGGAATATCGGCGGTG‑CC‑TTTTAACTCCAAATCCAGCCGGTCTTCATA‑GGGGAAGCGTGAAGAATCTTCTTTGACTAAAAACA‑GATGCAACCAGTCGCACTGTGCCGCAGCCTGTTGAATCAGATAACGGTGACCATTCGTAAAGTGATTGGCGTTCATCACAATGCAGCCAATCTTGTTCCCTGGATGa < 2:124280/248‑1 (MQ=255) aGCAAGGGAACGTAGCGCGGGAGATGATGTATTCGGAGCCACGATGCACAGTCAGGCGTGGAATATCGGCGGTG‑CC‑TTTTAACACCAAATCCAGCCGGTCTTCATA‑GGGGAAGCGTGAAGAATCTTCTTTGACTAAAAACA‑GATGCAACCAGTCGCACTGTGCCGCAGCCTGTTGAATCAGATAACGGTGACCATTCGTAAAGGGATTGGCGTTCATCACAATGCAGCCAATCTTGTTCCCTGGATGACg < 1:148324/250‑1 (MQ=255) cGTAGCGCGGGAGATGATGTATTCGGAGCCACGATGCACAGTCAGGCGTGGAATATCGGCGGGG‑CC‑TTTTTACACCAAATCCAGCCGGGCTTTATA‑GGGGGAGCGTGAAGAATCTTCTTTGACTAAAAACA‑AATGCAACCAGTCGCACTGTGCCGCCGCCTGTTGAATAAGATAACGGTGACCATTCCTAAAAGGGTTGGCGTTCATCACAATGCAGCCCAGCTTGGTTCCTGGGTGGCGAAAttttttt > 2:248499/1‑249 (MQ=255) gTAGCGCGGGAGATGATGTATTCGGAGCCACGATGCACAGTCAGGCGTGGAATATCGGCGGTG‑CC‑TTTTAACACCAAATCCAGCCGGTCTTCATA‑GGGGAAGCGTGAAGAATCTTCTTTGACTAAAAACA‑GATGCAACCAGTCGCACTGTGCCGCAGCCTGTTGAATCAGATAACGGTTACCATTCGTAAAGGGATTGGCGTTCATCACAATGCAGCCAATCTTGTTCCCTGGATGACGAAATTTTTTCAg < 1:169330/251‑1 (MQ=255) gTAGCGCGGGAGATGATGTATTCGGAGCCACGATGCACAGTCAGGCGTGGAATATCGGCGGTG‑CC‑TTTTAACACCAAATCCAGCCGGTCTTCATA‑GGGGAAGCGTGAAGAATCTTCTTTGACTAAAAACA‑GATGCAACCAGTCGCACTGTGCCGCAGCCTGTTGAATCAGATAACGGTGACCATTCGTAAAGGGATTGGCGTTCATCACAATGCAGCCAATCTTGTTCCCTGGATGACGAAATTTTTTCAg < 2:40934/251‑1 (MQ=255) cgGGAGATGATGTATTCGGAGCCACGATGCACAGTCAGGCGGGGAATATCGGCGGTG‑CC‑TTGTAACACCAAATCCAGCCGGTCTTCATA‑GGGGAAGCGTGAAGAATCTTCTTTGACTAAAAACA‑GATGCAACCAGTCGCACTGGGCCGCAGCCTGTTGAATCAGATAACGTTGACCATTCGTAAAGGGATTGGCGTTCATCACAATGCAGCCAATCTTGTTCCCTGGATGACGAAATTTTTTCAGCGATTc < 2:7134/251‑1 (MQ=255) gggAGATGATGTATTCGGAGCCACGATGCACAGTCAGGCGTGGAATATCGGCGGTG‑CC‑TTTTTACACCCCATCCACCCCGTCCTCATA‑GGGGGAGCGTGACCAATCTTCTTTGACTAAAACCAAGA‑GCAAACCGTCGCACTGTGCCGCAGCCTGTTTGATCAGATAACCGCGACCATTCGTCAACGGATTGGCGGTTATCACCATGAAGCCAAACTTGTTCCCTGGGTGACGGACTTTTTTCAGCGCTTCg > 1:209325/1‑251 (MQ=255) tATTCGGAGCCACGATGCACAGTCAGGCGTGGAATATCGGCGGTG‑CC‑TTTTTACACCAAATCCCGCCGGGCTTCATA‑GGGGAAGCGTGGAGAATCTTTTTTGACTAAAAACA‑AATGCAACCAGTCGCCCTGTGCCGCAGCCTGGTGAAACAGATAAAGGGGACCCATCGGAAAGGGATTGGGGTTCATCACAATGCAGCCCATCTTGTTCCCTGGGTGACGAAAATTTTTTAGCGATTCGGCATAGCGttt > 1:64584/1‑251 (MQ=255) gAGCCACGATGAACATTCAGGCGTGGATTATCGGCGGTG‑CC‑TTTTAACACCAAATCCAGCCGGTTTTCATA‑GGGGAAGCGTGAAGAAGCTTCTTTGACTAAAAACA‑GATGCAACCAGTCGCACTGTGCCGCAGCCTGTTTAATCAGATAACGGTGACCATTCGTAAAGGGATTGGTGGTCATCACAATGCATCCAATCTTGTTCCCTGGATGACGAAATTTTTTCAGCGATTCGGCATAGCGTTTCAGTcg < 1:269907/251‑1 (MQ=255) ggCCACGATGCACAGTCAGGCGTAGAATATCGGCGGTG‑CC‑TTTTAACACCAAATCCAGCCGGTCTTCATA‑GGGGAAGCGTGAAGAATCTTCTTTGACTAAAAACA‑GATGCAACCAGTCGCACTGTGCCGCAGCCTGTTGAATCAGATAACGGTGACCATTCGTAAAGGGATTGGCGTTCATCACAATGCAGCCAATCTTGTTCCCTGGATGACGAAATTTTTTCAGCGAGTCGGCATAGCGTTTCAGTcgc < 2:190746/250‑1 (MQ=255) gTCAGGCGTGGAATATCGGCGGTG‑CC‑TTTTTACAACAAATCCAGCCGGTCTTTATA‑GGGGAAGGGTGAAGAATCTTCTTTGAATAAAAAAA‑AATGAAACCAAGCGCACTACGGCGCCGGCTGTTGAATCAGATAAAGGGGACAATTAGTAAAGGGAATGGGCTTCATCACAAAGCAACCAATCTTGTTTTCCGGGTGAAGAAATTTTTTCACCGCTTTGGCATAACGTTTCAGTCGCGTGGAGCTGTTTTc > 1:327872/1‑251 (MQ=255) gTCAGGCGTGGAATATCGGCGGTG‑CC‑TTTTAACACCAAATCCAGCCGGTCTTCATA‑GGGGAAGCGTGAAGAATCTTCTTTGACTAAAAACA‑GATGCAACCAGTCGCACTGTGCCGCAGCCTGTTGAATCAGATAACGGTGACCATTCGTAAAGGGATTGGCGTTCATCACAATGCAGCCAATCTTGTTCCCTGGATGACGAAATTTTTTCAGCGATTCGGCATAGCGTTTCAGTCGCGTGGCGCTGTTTTc < 1:225583/251‑1 (MQ=255) aGGCGTGGAATATCGGCGGTG‑CC‑TTTTAACACCAAATCCAGCCGGTCTTCATA‑GGGGAAGCGTGAAGAAGCTTCTTTGACTAAAAACA‑GATGCAACCAGTCGCACTGTGCCGCAGCCTGTTGAATCAGATAACGGTGACCATTCGTAAAGGGATTGGCGTTCATCACAATGCAGCCAATCTTGTTCCCTGGATGACGAAATTTTTTCAGCGATGCGGCATAGCGTTTCAGTCGCGTGGCGCTGTTTTCCAt < 2:269635/251‑1 (MQ=255) atatCGGCGGTG‑CC‑TTTTAACACCAAATCCAGCCGGTCTTCATA‑GGGGAAGCGTGAAGAATCTTCTTTGACTAAAAACA‑GATGCAACCAGTCGCACTGTGCCGCAGCCTGTTGAATCAGATAACGGTGACCATTCGTAAAGGGATTGGCGTTCATCACAATGCAGCCAATCTTGTTCCCTGGATGACGAAATTTTTTCAGCGATTCGGCATAGCGTTTCAGTCGCGTGGCGCTGTTTTCCATCAGCACCAt < 1:9858/251‑1 (MQ=255) ggcggTG‑CC‑TTTTAACACCAAATCCAGCCGGTCTTCATA‑GGGGAAGCGTGCAGAATCTTCTTTGACTAAAAACA‑GATGCAACCAGTCGCACTGTGCCGCAGCCTGTTGAATCAGATAACGGTGACCATTCGTAAAGGGATTGGCGTTCATCACAATGCAGCCAATCTTGTTCCCTGGATGACGAAATTTTTTCAGCGATTCGGCATAGCGTTTCAGTCGCGTGGCGCTGTTTTCCATCAGCa > 1:405717/1‑242 (MQ=255) ggcggTG‑CC‑TTTTAACACCAAATCCAGCCGGTCTTCATA‑GGGGAAGCGTGAAGAATCTTCTTTGACTAAAAACA‑GATGCAACCAGTCGCACTGTGCCGCAGCCTGTTGAATCAGATAACGGTGACCATTCGTAAAGGGATTGGCGTTCATCACAATGCAGCCAATCTTGTTCCCTGGATGACGAAATTTTTTCAGCGATTCGGCATAGCGTTTCAGTCGCGTGGCGCTGTTTTCCATCAGCa < 2:405717/242‑1 (MQ=255) ggTG‑CC‑TTTTAACACCAAATCCAGCCGGTCTTCATA‑GGGGAAGCGTGAAGAATCTTCTTTGACTAAAAACA‑GATGCAACCAGTCGCACTGTGCCGCAGCCTGTTGAATCAGATAACGGTGACCATTCGTAAAGGGATTGGCGTTCATCACAATGCAGCCAATCTTGTTCCCTGGATGACGAAATTTTTTCAGCGATTCGGCATAGCGTTTCAGTCGCGTGGCGCTGTTTTCCATCAGCCCCATCACGCCgg > 1:412186/1‑251 (MQ=255) tAACACAAAATCCAGCCGGTCTTCATA‑GGGGAAGCGTGAAGAATCTTCTTTGACTAAAAACA‑GATGCAACCAGTCGCACTGTGCCGCAGCCTGTTGAATCAGATAACGGTGACCATTCGTAAAGGGATTGGCGTTCATCACAATGCAGCCAATCTTGTTCCCTGGATGACGAACTTTTTTCAGCGATTCGGCATAGCGTTTCAGTCGCGTGGCGCTGTTTTCCATCAGCACCATCACGCCGGGTACGCTgg > 2:190597/1‑251 (MQ=255) | CAGCCAGTAGCGCATATCCTGGTTGTACTGGGCGGTAACGCGACAAAAGGGTTCAGTACCGACAAAGCGGTGAGTTACACCCAGCGCGGGAGCGAGGTACTGACGGAAAATCTTCAGATCAATTTCGGTGTAACAATGGTTAATGACGCTCTGTTCTTTAATGAAGTAGCAAGGGAACGTAGCGCGGGAGATGATGTATTCGGAGCCACGATGCACAGTCAGGCGTGGAATATCGGCGGTG‑CC‑TTTTAACACCAAATCCAGCCGGTCTTCATA‑GGGGAAGCGTGAAGAATCTTCTTTGACTAAAAACA‑GATGCAACCAGTCGCACTGTGCCGCAGCCTGTTGAATCAGATAACGGTGACCATTCGTAAAGGGATTGGCGTTCATCACAATGCAGCCAATCTTGTTCCCTGGATGACGAAATTTTTTCAGCGATTCGGCATAGCGTTTCAGTCGCGTGGCGCTGTTTTCCATCAGCACCATCACGCCGGGTACGCTGG > NC_000913/651020‑651516 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |