Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,560,455	+G	intergenic (+589/+167)	rtcR → / ← glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,560,455	1	.	G	100.0%	162.0 / NA	48	intergenic (+589/+167)	rtcR/glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (18/30);  total (18/30)

AACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCT‑CCGCCACTTT‑GCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGT  >  NC_000913/3560221‑3560694                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                aaCGTGTTAGCAACTTGGAGATTGTTGGTGAAAATGCGAAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATGGGGGATTTGCT‑CCGCCACTTT‑GCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTg                                                                                                                                                                                                                                  <  1:389802/251‑1 (MQ=255)        tAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCT‑CCGCCACTTT‑GCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGcc                                                                                                                                                                                                                           >  2:78046/1‑251 (MQ=255)                        gttgGTGACAATGCGCAAATTGTTGTGATTGAGCAGTGCTTGCGCTACCGCTCCCGGCGTGGTGCCGATATCTATAAACAGCGCCGAGCCATTGGGGATTTGCT‑CCGCCACTTT‑GCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACGGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTcg                                                                                                                                                                                                            <  1:253525/250‑1 (MQ=255)                             tGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCT‑CCGCCACTTT‑GCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcggcg                                                                                                                                                                                                       >  1:36044/1‑250 (MQ=255)                              gACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCT‑CCGCCACTTT‑GCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGCAGGCAGCGCCGCACCGCCATGATGGCGCAGGCTCAGGTTTTGCTCCGCCAGCTCATTGAGCTCGCGGCGa                                                                                                                                                                                                      >  2:47379/1‑250 (MQ=255)                                    gcgcAAATTGCTGTGATTGAGCAGAGCGTGCGCTACCGATTCCGGCGTGGCGCCGATATCGATAAACAGCGACGAGCCATTGGGGATTTGCT‑ACGCCACTTT‑GCGGGCGATACGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGAGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGATTTGCTACGCCAGCTCAttaagcgtggtgcgaatactct                                                                                                                                                                                               >  2:282149/1‑231 (MQ=255)                                         aaTTGCTGTGATTGAGCAGTCCTTGCGCTCCCCTTTCCGGCGTGGTCCCGATATCGATAAACAGCGTCGA‑CCCTTTGGGATTTTCC‑CCCCCCCTTTTGCGGGCGATGCGCTCTTTTTCTCCGGTCTGGGTGGCCTTGCGATCGTGCCACGTCGTTTTAACCGAACTGGAAGGCAGCGCCGCTCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCggg                                                                                                                                                                                          <  1:1974/251‑1 (MQ=255)                                            tgttgggattgcgcggggcgggcgcTCCCGCTTCCGGCGTGGTGCCGTTATCGATAACCAGCGTCGAGCCTTTGGGGATTTGCT‑CCGCCACTTT‑GCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCt                                                                                                                                                                                        <  1:94995/229‑1 (MQ=255)                                                gtgATGGAGCGGTGCGGGCGCTCCCGCTCCCGGCGTGGTGCCTATATCGTAAAACAGGGTCGAGCCATGGGGGTTTTGCT‑CCGCCCCTTG‑GCGGGCGATGCGCTTTTTTTCTTCGGGCTGGTTGGCCTTGCGATCGGGCCACGGGGTGTTAACCGAACTGGAAGGCATCGCCGCACCGCCATGATGGCGCAGGATCAGGTTGTGCTGCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACgg                                                                                                                                                                                   <  1:252241/251‑1 (MQ=255)                                                 ggATTGAGCAGTGCGTGCTCTCCCGCTTCCGGCGTGGTGCGGATATCGAAAAACCGCGTCGAGCCTTTGGGGATTTGCT‑CCGCCACTTT‑GCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGga                                                                                                                                                                                  <  1:113348/250‑1 (MQ=255)                                                     tGAGCAGTGCGAGCGCTACCGCATACGGCATGGTACCGATATCGATAAACAGCGTCGAGCCTTTGAGGATTTGCT‑CCGCCACTTC‑GCGAGCGATGCGCTCTTTTTCTTCGGTCAGGGTGGCCTTGCGCTCGTGCCACGGCGTGTTAACAGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCACTGCGGTCGAGGCGAATAGTCGGCGGGCTGACGGAGaa                                                                                                                                                                               >  2:82344/1‑250 (MQ=255)                                                                              gggggtggggccaaaatCGATAAACACCGCCTACCCGTTGGGGATTTCCC‑CCGCCACTTG‑GCGGGCGTTGCGCTTTTTTTCTTCGGTTTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGa                                                                                                                                                     <  2:248721/236‑1 (MQ=255)                                                                                         cGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCT‑CCGCCACTTT‑GCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGCACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgc                                                                                                                                          >  2:58030/1‑251 (MQ=255)                                                                                         cGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCT‑CCGCCACTTT‑GCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgc                                                                                                                                          >  1:394410/1‑251 (MQ=255)                                                                                          gATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCT‑CCGCCACTTT‑GCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTg                                                                                                                                                                                                                  >  1:41513/1‑178 (MQ=255)                                                                                           atatCGATAAACAGCGTCGAGCCATTGGGGATTTGCT‑CCGCCACTTT‑GCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgctg                                                                                                                                        >  1:373583/1‑251 (MQ=255)                                                                                            tatCGATCAACAGCGTCGAGCCATTGGGGATTTGCT‑CCGCCACTTT‑GCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGt                                                                                                                                       >  2:298120/1‑251 (MQ=255)                                                                                             gccgaaaaaacgCGTGGAGCCATTGGGGATT‑GCTGCCGCCGCTTT‑GCGGGCGATGCGCTCTTTGTCTTCGGCCTGGGTGGCCTGGCGATCGAGCAACGGGGTGTTAAACGACCGGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTTCTGACATAACCCTGCTGtt                                                                                                                                      <  1:434789/240‑1 (MQ=255)                                                                                                  aaaaaggggtggggctaTTGGGGGTTGGCC‑CCGCCATTGT‑GCGGGCGATGCGCTCTTTTTCTTCGGTGTGGGGGGCCTGGTGATCGTGCCACGGCGTGTTAACCGACCTGTAAGGCAGCGCCGCCCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAcc                                                                                                                                 <  2:250992/235‑1 (MQ=255)                                                                                                   aaccAGCGCCGTGCCATTGGGGATTTGCT‑CCGCCACTTT‑GCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCGGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCa                                                                                                                                <  1:180230/248‑1 (MQ=255)                                                                                                              acgcttcggggCTTTGCT‑CCCCCACTTT‑GCGGGCGATGCGCTCTTTTTCTTCGGTCGGGGTGGCCTTCGGAGCGTGCCACGGCTTGTTAACCGATGTGGAAGGCAGGGCTCCCCCGCCATGATGGCGCAGTATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGGTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataata                                                                                                                     <  1:261258/244‑1 (MQ=255)                                                                                                                gctatgggggatgcgctccgctgtgtt‑GCGGGCGATGCTCTCTTTGTCGTCGGTCTGGGTGGCCTGGCGATCGTGCCACGGCGTGTTGACGGACCTGGAAGCCAGCGCTGCCCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAc                                                                                                                    <  2:139524/226‑1 (MQ=255)                                                                                                                     ggggTTTTGGT‑CGGCCACTTT‑GGGGGTGATGCGCTCTTTTTCGTCGGGCGGGGGGGCCTTGCGATCGTGCCACGGCGTGTAAACCGAACTGGAGGGCAGCTCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCGGCTGTTTAACCAGTTCGATAATACCGTtgt                                                                                                              <  1:357156/251‑1 (MQ=255)                                                                                                                           ttGCC‑CCGCCACTTT‑GCGGGCGATGCGCTCTTTTTCTGCGGTCTGGGGGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGt                                                                                                         <  1:298120/250‑1 (MQ=255)                                                                                                                              tgcccGCCACTTT‑GCGTTCGAGTCGCTCTTTTCCGTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCTTGTTACCCGACCTGGAAGGCCGCGCCGCCCCGCCCTGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCTGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTtg                                                                                                       <  1:248760/247‑1 (MQ=255)                                                                                                                                   gCCACTTT‑GCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgtttg                                                                                                 >  1:435836/1‑251 (MQ=255)                                                                                                                                      aCTTT‑GCGGGCGAGGCGCTCTTCTCCTCCGGTGTGGGGGGCCTTGCGACCGGTCCGCGGCGTGTTAACCCACCTGAAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGTTCCGCCAGCTCATGGAGGTCGCGGCGAATATTCTGCGGGCTGACGGAGAAATGCTCTGCCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAAGTCCGTTGTGACGTTGTgtttgttt                                                                                              <  2:318599/251‑1 (MQ=255)                                                                                                                                         tt‑GCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGTCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTc                                                                                             <  1:67082/249‑1 (MQ=255)                                                                                                                                           ggCGGGCGATGCGCTCTTTTCCTTCGGGCGGGGTGGCCTGGCGACCGTGCCACGGCGGGTTAACCGAACTGAAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCAt                                                                                           <  2:74650/249‑1 (MQ=255)                                                                                                                                            gCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCAttt                                                                                         >  1:252062/1‑251 (MQ=255)                                                                                                                                                    ggcgcTCTTTTTCTTCGGTCTGGGGGGCCTTGCGCTCGGGCCACGGCGTGTTAACCGAACGGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACTGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGGGACGTTGTGTTTGTTTCATTTATAAATcc                                                                                 <  2:24632/250‑1 (MQ=255)                                                                                                                                                             tgtctgcGGTCGGGGTGGCCTTGCGATCGTGCCACGGCGTGTGAACCGATCGGGAAGGCAGCGCCGCACCGCCATGATGGCGCATGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAatt                                                                         <  2:157558/244‑1 (MQ=255)                                                                                                                                                               tcttcGGTCTGGGTGGCCTTGCGATCTGGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAattat                                                                       <  1:58030/250‑1 (MQ=255)                                                                                                                                                                  tcGGTCTGGGGGGCGTTGCGTTCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttt                                                                     <  1:420305/249‑1 (MQ=255)                                                                                                                                                                   cGGTCGGGGTGGCCTTGCGATCGTTCCACGGCTTGGTAACCGAACTGGAAGGCAGCGCCGCCCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTTACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcg                                                                  <  2:313513/251‑1 (MQ=255)                                                                                                                                                                    ggTCTGGGTGGCCTGGCGATCGTGCCACGGGGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgt                                                                 <  1:145865/251‑1 (MQ=255)                                                                                                                                                                     gTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGAACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgtt                                                                >  2:385575/1‑251 (MQ=255)                                                                                                                                                                                      aTCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCACTGGAATTATTTTCGTTTTCGCGCATTGAGCGa                                                >  1:203180/1‑250 (MQ=255)                                                                                                                                                                                                      ttAACCGACCTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATc                                <  1:275818/250‑1 (MQ=255)                                                                                                                                                                                                        aaCCGACCTGAAAGGAAGCGTCGCACCGCCATGATGGCGCAGGATCATGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGGCGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATATCCCTGCTGTTTAACCAGTTCGATAATTCCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGTAAGGATTTTCGGTTGCGCGCATTGAGCGAATCAACAAAATCCATCGcg                             <  2:232088/251‑2 (MQ=255)                                                                                                                                                                                                          ccGAACTGGTAGGAAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTa                            >  1:137573/1‑250 (MQ=255)                                                                                                                                                                                                           cGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTaaa                          <  2:373583/251‑1 (MQ=255)                                                                                                                                                                                                                 ggAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGCCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAACCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACg                    >  1:179049/1‑251 (MQ=255)                                                                                                                                                                                                                  gAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGGATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACgg                   >  1:299421/1‑251 (MQ=255)                                                                                                                                                                                                                         gcgcCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAAccc            <  2:113742/251‑1 (MQ=255)                                                                                                                                                                                                                           gccgcACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCCCGGCTAACCCgg          >  2:124690/1‑251 (MQ=255)                                                                                                                                                                                                                                  cGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGAtgtg   <  2:36044/251‑1 (MQ=255)                                                                                                                                                                                                                                   gCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGAtgtgt  <  1:211893/251‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                AACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCT‑CCGCCACTTT‑GCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGT  >  NC_000913/3560221‑3560694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑