Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,156,913	G→A	G46D (GGT→GAT)	ycfH →	putative metal‑dependent hydrolase YcfH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,156,913	0	G	A	100.0%	229.7 / NA	71	G46D (GGT→GAT)	ycfH	putative metal‑dependent hydrolase YcfH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (41/30);  total (41/30)

GTTACAGGCATCAACCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCC  >  NC_000913/1156668‑1157153                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 gTTACAGGCATCAACCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGCCATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAAAATTACCGGattatt                                                                                                                                                                                                                                             >  2:389286/1‑251 (MQ=255)     cAGGCATCAACCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTAc                                                                                                                                                                                                                                          >  2:30118/1‑250 (MQ=255)     cAGGCAGCAACCGCGAGCTTCTCATCACCGATCTTTTACTGCTTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACa                                                                                                                                                                                                                                         <  2:460035/251‑1 (MQ=255)          aTCAACCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATAATTTACATATgt                                                                                                                                                                                                                                    >  1:500401/1‑250 (MQ=255)           ccAACCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAAGCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCg                                                                                                                                                                                                                                   <  2:497869/250‑1 (MQ=255)            cAACCGCGAGCTGCTCAGCACCGATCTTTTACTGCGTATTTAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCAGGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATGATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACTCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGTATTATTTACATATGCg                                                                                                                                                                                                                                   <  2:607194/250‑1 (MQ=255)              aCCGAGAGCTTCTCACCACCGATCTTTTACTGCTTATTGAGCATTACCTGCAACCGGGCGTGGTGCTACCGTTTCCTCATCTGTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTGTGTCTGGCAGTCGCCACATCATTACCGGATTATTTACATATGCGGGa                                                                                                                                                                                                                                <  2:30904/251‑1 (MQ=255)                  cgAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTg                                                                                                                                                                                                                            >  2:159268/1‑251 (MQ=255)                   gAGCTTCTCATCACCGATCTTTTAATGCGTCTTGCGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTgg                                                                                                                                                                                                                           >  2:492478/1‑251 (MQ=255)                        tctcATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGc                                                                                                                                                                                                                      >  1:225079/1‑251 (MQ=255)                                 cGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGAGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCCTCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCCCGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGGGAc                                                                                                                                                                                                             >  2:96979/1‑251 (MQ=255)                                      ttttACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGTCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCTGGATCTGGTAGGCGACCGTGACAAcgt                                                                                                                                                                                                        >  1:223086/1‑251 (MQ=255)                                         tACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAAcgtcgt                                                                                                                                                                                                     >  1:246371/1‑251 (MQ=255)                                          aCTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAAcgtcgt                                                                                                                                                                                                     >  1:738491/1‑250 (MQ=255)                                                  ttGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTAACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTtg                                                                                                                                                                                            >  2:703516/1‑251 (MQ=255)                                                    gAGCATTACCTGCAACCGGGCGTTTTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTtgtg                                                                                                                                                                                          <  1:136807/251‑1 (MQ=255)                                                       cATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCg                                                                                                                                                                                       >  1:662727/1‑251 (MQ=255)                                                       cATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTGTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACATCGTCGTATTTTCGTGTGGCg                                                                                                                                                                                       <  2:34213/251‑1 (MQ=255)                                                           aCCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGc                                                                                                                                                                                    <  1:582420/250‑1 (MQ=255)                                                            ccTGCAACCGGGCGTTGTGCTACCGGTTCCTCAGCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCGGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGAGCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCAt                                                                                                                                                                                  <  2:736579/251‑1 (MQ=255)                                                                  aCCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTc                                                                                                                                                                            <  1:159268/251‑1 (MQ=255)                                                                     gggCGTTGTGCTACCGGTTCCTCATCTTTAAGCGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGACGTCTTTTCTTGTGGCGTGCATCCACTCAAc                                                                                                                                                                         >  2:442806/1‑251 (MQ=255)                                                                      ggCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGCATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAAcc                                                                                                                                                                        >  1:349778/1‑251 (MQ=255)                                                                      ggCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTTGCGTGCATCCACTCAAcc                                                                                                                                                                        <  2:121132/251‑1 (MQ=255)                                                                       gCGTTGTGCTACCGGTTCCTCATCGTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTTTTTTCTTGTGGCGTTAATCCACTCAACCa                                                                                                                                                                       <  1:238577/251‑1 (MQ=255)                                                                            gtgCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATg                                                                                                                                                                  >  2:157085/1‑251 (MQ=255)                                                                            gtgCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATg                                                                                                                                                                  >  2:193196/1‑251 (MQ=255)                                                                               cTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGCACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATc                                                                                                                                                               >  2:56738/1‑251 (MQ=255)                                                                               cTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATc                                                                                                                                                               >  1:131689/1‑251 (MQ=255)                                                                                tACCGGTTCCTCATCTATAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGTTGGTCTGGATTATGATTCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGACGCAGCACGCGATGTGAACTTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTTGTATTTTCTTGTGGCGTGCTTCCACTCAACCAGACTGATcc                                                                                                                                                              >  2:414007/1‑251 (MQ=255)                                                                                             tCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTaga                                                                                                                                                  >  1:631943/1‑250 (MQ=255)                                                                                                ttAAGAGAGACAGCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGGCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATGACCGGATTATTTACATATGCGGGATCTTGTAGGCGAACGTGACAACGTCTTATTTTCTTGTGGCGTGCAGCCACTCAACCAGAATGATCCCTACGATTGagaaga                                                                                                                                               <  2:477610/250‑1 (MQ=255)                                                                                                    gagagaCATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTa                                                                                                                                           >  2:427391/1‑250 (MQ=255)                                                                                                      gagaCATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCt                                                                                                                                                                                                                             <  2:390922/166‑1 (MQ=255)                                                                                                      gagaCATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCt                                                                                                                                                                                                                             >  1:390922/1‑166 (MQ=255)                                                                                                           atcatGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCt                                                                                                                                   <  1:389286/251‑1 (MQ=255)                                                                                                                gTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTggcgg                                                                                                                              <  1:511272/251‑1 (MQ=255)                                                                                                                 tttttCGTCGACTCAAACTGCCATCTCGAAGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTggcggc                                                                                                                             >  1:256987/1‑251 (MQ=255)                                                                                                                   tttcgTCGCCTCACACTCCCATCTCGATGTTCCGGATTATGCATCTTTGCATAAGGACGTGGATGCCGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTGACATATGCGGGATCTGGTCGGCGAACGTGCCATCGTCGTATTTTCTTGTGGCGTGCATCCACTTAACCAGACTGATACCTACGATGTAGAAGATTTACGCCGTCTggcgtc                                                                                                                             >  2:40961/5‑247 (MQ=255)                                                                                                                       gTCGACTCCCACTGACATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCaga                                                                                                                          >  2:699297/1‑248 (MQ=255)                                                                                                                       gTCGACTCACTCTGCCACCTCGATGGTCTGGATTAGGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCGGGCAGTCGCCACAACATTTCCTGATTATTTACATATGCGTGATCTGGTAGGCGAACGTGACTACGTCGTATTTGTTTGTGGCGTGCATCCACTCAGCCAGATGGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCagaaga                                                                                                                       <  1:429173/251‑1 (MQ=255)                                                                                                                       gTCGACTCACACTGCCATCTCGATGGTATGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCaga                                                                                                                          >  1:489934/1‑248 (MQ=255)                                                                                                                         cGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAgg                                                                                                                     >  1:643656/1‑251 (MQ=255)                                                                                                                              cacaCTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGtgtt                                                                                                                >  1:360564/1‑251 (MQ=255)                                                                                                                                     ccATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGGGTTGTAgcgc                                                                                                         >  2:675249/1‑251 (MQ=255)                                                                                                                                     ccATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGACGCACGAGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGCAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGGTGTAgcgc                                                                                                         >  1:374202/1‑251 (MQ=255)                                                                                                                                       aTCTCGATGGTCTGGATTATGAATCTTTGCATAAGGCCGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGCACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATTATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTg                                                                                                       >  2:538967/1‑251 (MQ=255)                                                                                                                                                                 ttGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGGGAAACCGGGCTGGattattatt                                                                              >  1:715531/1‑250 (MQ=255)                                                                                                                                                                       aaGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTagaa                                                                                                                                                 >  1:650376/1‑177 (MQ=255)                                                                                                                                                                       aaGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTagaa                                                                                                                                                 <  2:650376/177‑1 (MQ=255)                                                                                                                                                                        aGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCg                                                                      >  2:330337/1‑251 (MQ=255)                                                                                                                                                                         ggACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTAACCGCCgg                                                                     >  2:278441/1‑251 (MQ=255)                                                                                                                                                                             gTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAAc                                                                 <  1:157085/251‑1 (MQ=255)                                                                                                                                                                                gATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTa                                                               >  2:314440/1‑250 (MQ=255)                                                                                                                                                                                   gACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAg                                                            <  1:96979/250‑1 (MQ=255)                                                                                                                                                                                           ggCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTACGTCAg                                                    <  2:9671/250‑1 (MQ=255)                                                                                                                                                                                           ggCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTACGTCAGc                                                   >  1:84390/1‑251 (MQ=255)                                                                                                                                                                                            gCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGGTTATTATTACACGcc                                                                       <  2:481409/230‑1 (MQ=255)                                                                                                                                                                                            gCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGGTTATTATTACACGcc                                                                       >  1:481409/1‑230 (MQ=255)                                                                                                                                                                                                  gccgccGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAATGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTACGTCAGCAAGAGTc                                            <  1:30118/251‑1 (MQ=255)                                                                                                                                                                                                       cgcACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTACGTCAGCAAGAGTCCTTca                                       <  1:442806/251‑1 (MQ=255)                                                                                                                                                                                                                 gttaaaTTTTGTCTGGCAGTCGCCACAACATTACCGGATTAGTTACATATGCGGGATCTGGTAGTCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGTGTGTTGTAGCGCTGGGTGAAACCTGGCTGGATTATTATGACACGCCGGAAACTAAAGGCCGTCAGCAAGATTCCTTCAGCCATCata                              <  2:359503/247‑1 (MQ=255)                                                                                                                                                                                                                      aTTTTGTCTGGCAGGCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGa                        <  2:225079/251‑1 (MQ=255)                                                                                                                                                                                                                         ttGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGAtt                      <  1:703516/250‑1 (MQ=255)                                                                                                                                                                                                                          tGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGTCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTACGTCAGCAAGAGTCCTTCTTCCATCATATCCAGATTg                     <  1:187527/250‑1 (MQ=255)                                                                                                                                                                                                                           gTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTggg                   >  1:239714/1‑250 (MQ=255)                                                                                                                                                                                                                               ggCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTc                  <  1:492478/248‑1 (MQ=255)                                                                                                                                                                                                                               ggCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGt                <  1:193196/250‑1 (MQ=255)                                                                                                                                                                                                                                  ggTCGCCACAACATTACCGGATTATTTACATATGAGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGtgaac            >  1:571657/2‑251 (MQ=255)                                                                                                                                                                                                                                        cacaACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTACGTCAGCAAGAGTACTTCATCCATCATATCCAGATTGGTCGTGAACTGAACa      <  2:485714/251‑1 (MQ=255)                                                                                                                                                                                                                                          caacaTTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGGGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACa      >  2:406235/1‑249 (MQ=255)                                                                                                                                                                                                                                            acaTTCCCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAAAGTCGTATTTTCTTTTGGCGTGTATCCACTCAACCTGAATTATCCCTACGTTTTTGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTTTAGCGCTGGGTGAAAACGGGCTGGTTTATTATTACACGTCGGAAATTTAAGTACGTCAGCAAGATTCCTTCATCCATCATATCCAGATTGGTAGTTAACTGAACaatcc  >  2:693151/1‑248 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 GTTACAGGCATCAACCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCC  >  NC_000913/1156668‑1157153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑