BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	696,470	C→T	noncoding (35/75 nt)	glnX ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	696,470	0	C	T	95.7%	149.7 / NA	47	noncoding (35/75 nt)	glnX	tRNA‑Gln
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (21/24); minor base G (0/2); total (21/26)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

GGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGG‑CTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTAT  >  NC_000913/696232‑696705

ggCGTGTTGAAACGGCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGG‑CTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATGAGAATCCGGt                                                                                                                                                                                                                                   <  2:179587/249‑1 (MQ=255)

     gtTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGG‑CTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTAc                                                                                                                                                                                                                            >  1:467289/1‑251 (MQ=255)

       tGAAACGTCAGGCGCTGAAATATGCGTGAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGG‑CTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTAcc                                                                                                                                                                                                                           <  2:120393/250‑1 (MQ=255)

        gAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGG‑CTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCg                                                                                                                                                                                                                          <  2:534465/250‑1 (MQ=255)

             gTCAGGCGCTGAAATATGCGTTAGGTTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGATCTTTTTTAA‑GTGGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGc                                                                                                                                                                                                                    <  1:479396/251‑1 (MQ=255)

                   cgcTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCCGCGTCGCATCCGACATTGACGGATAAGCCGTGTCAAAATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGATCGCAGAAGCGAGCTTTTTTAATGTGG‑CTGGGGTACGAGGATTCGACCCTCGGAATGACGGAATTAAAATCCGGCGACTTACCGCTTGGAGATAcc                                                                                                                                                                                                              >  1:697949/1‑251 (MQ=255)

                       gAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGG‑CTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCa                                                                                                                                                                                                           >  2:168111/1‑250 (MQ=255)

                        aaaTATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGG‑CTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAAc                                                                                                                                                                                                         <  1:168111/251‑1 (MQ=255)

                          atatGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGG‑CTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTg                                                                                                                                                                                                       <  2:717173/251‑1 (MQ=255)

                            atGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGG‑CTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTggg                                                                                                                                                                                                     <  2:59764/251‑1 (MQ=255)

                                gTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGG‑CTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCa                                                                                                                                                                                                 <  2:635342/251‑1 (MQ=255)

                                  tAGGGATGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTAGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGG‑CTGGGGTACGAGAATTCAAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGccct                                                                                                                                                                                               >  1:90293/1‑248 (MQ=255)

                                     ggTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGG‑CTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTAc                                                                                                                                                                                            <  2:233694/251‑1 (MQ=255)

                                         gTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGG‑CTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACa                                                                                                                                                                                           >  2:405954/1‑248 (MQ=255)

                                         gTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGG‑CTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAg                                                                                                                                                                                         <  1:405954/250‑1 (MQ=255)

                                          tAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGG‑CTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGt                                                                                                                                                                                       >  2:184250/1‑251 (MQ=255)

                                           aGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGG‑CTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTa                                                                                                                                                                                      <  2:164338/251‑1 (MQ=255)

                                                tACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGG‑CTGGGGTACGATGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGc                                                                                                                                                                                   <  1:708486/249‑1 (MQ=255)

                                                                gCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGG‑CTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCt                                                                                                                                                                 <  1:586876/251‑1 (MQ=255)

                                                                  gTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGG‑CTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAGGGTGCAATTGTATGGTAAGCGACTTGAATAAATTgact                                                                                                                                                                 >  2:184361/1‑246 (MQ=38)

                                                                      cAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGG‑CTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTa                                                                                                                                                           <  1:633752/251‑1 (MQ=255)

                                                                       aaCCGTAGGTCGGCTAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGCCGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGG‑CTGGGGGACGAGGATTCGAACCACGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATCACCCAACCGGACGCCCTTACCAGGTCAGCGTCTTGAATAGATTTGCGgggggac                                                                                                                                                          >  2:136693/1‑248 (MQ=255)

                                                                       aaCCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGG‑CTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTAc                                                                                                                                                          <  2:460471/251‑1 (MQ=255)

                                                                                          cgcgGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGG‑CTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGGACTTACAAGGTAAGCGTCTTGAATAAATTTGCTGGGGTACGAGGATTCGAACCTCGGa                                                                                                                                        >  2:140572/1‑250 (MQ=255)

                                                                                              gCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGT‑CTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCGTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGcc                                                                                                                                   <  1:684410/251‑1 (MQ=255)

                                                                                                           cGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGG‑CTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                                                                      >  1:9292/1‑251 (MQ=255)

                                                                                                                  gAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGG‑CTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACATGGTACGCGTCTTGAATAAATTGGCTGGGGTTCGAGGATTCGAACCTAGGAGTGCCGGAATCAGAATCAGGTTCCt                                                                                                               >  2:56526/1‑251 (MQ=38)

                                                                                                                    aGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGG‑CTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGTTACGAGGCTTCGAACCTCGGAATGCCGGAATCAGCATCCGTTGCCtt                                                                                                              >  2:358513/1‑250 (MQ=255)

                                                                                                                    aGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGG‑CTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTa                                                                                                             <  1:184250/251‑1 (MQ=255)

                                                                                                                    aGGATAAGACGTGTCAACAGCGCAATCGGTATTGAATGAACGCAGAAAAGCAAAAAGCACGCCGAAGCGACCTTTTTTAAAGTGG‑CTGCGGTACGAGGATTCGAACCTCGGAATGACGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTa                                                                                                             <  2:467289/251‑1 (MQ=38)

                                                                                                                      gtgaaGACGTGTCAACAGCGCATTCGACTTTGAATGAACGCAGAAAAGAAAAAAGCTCGCCGAAGCGAGCTTTGTTAATGTGG‑CTGGGGTACGAGGATTCGAACCACGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTAcc                                                                                                           <  2:2239/248‑1 (MQ=38)

                                                                                                                                gtCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGG‑CTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGa                                                                                                  <  1:536412/250‑1 (MQ=255)

                                                                                                                                  caacttcgaattcggtaatgaatgaaCGCAGAAAAGCAAAACGGACGCCGAAGCGCGCTTTTTTAATTTGG‑CTGGGGTACGAGGATTCGAACCTCTGAATGCCGGAATTAGAATCCGGTGCCTTCCCGCTTGGCGATACGCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATa                                                                                                <  2:181259/232‑1 (MQ=37)

                                                                                                                                         gCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGG‑CTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCAGAATCAGAATCCGGTGCCTTTCCGCTTGCCGATACCCcaac                                                                                         >  1:397956/1‑250 (MQ=255)

                                                                                                                                           aTTCGACCTTGCATGAACGCAGAAACGCAAAAAGCTCGCCGAAGCGACCATTTTTAATGTGG‑ATGGGGTACGAGGATTCGAACCTCGGAATGCCGGACTTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTAGGGGCACTTACAAGGTAAGAGTCTTCAATAAATCGGCTGAGTCACGAGGATTCAAACCTGGGAATACCGGAATCAAAATCCGGGGCCTTACCGCGTGGCGATACCCcaccaac                                                                                      >  2:318260/1‑250 (MQ=21)

                                                                                                                                             tCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGG‑CTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                                                                                     <  1:739324/250‑1 (MQ=255)

                                                                                                                                                            cgctgAAAAGCAAAAAGCACGCCGAAGCGAGCTATTTTAAAGGGG‑CTGGGGTACGAGGATTCGAAACTCGGAATGCCAGAATGAGAATACGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGcc                                                                     <  2:555740/247‑1 (MQ=38)

                                                                                                                                                                 aaaaGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGG‑CTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGAAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACACCAACAAATTGGTTTTGAATTTGACGAAc                                                                 >  1:384474/1‑250 (MQ=255)

                                                                                                                                                                           aGCTCGCCGAAGCGAGCTTTTTTAATGTGG‑CTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTCCAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATACCGGAATCAGAATCCGGTGCCTTGCCGCTTGGCGATACCCCAACAAATTGGTTTTGATTTTGCCGATCATATTCGATAc                                                      >  1:21675/1‑251 (MQ=255)

                                                                                                                                                                            gCTCGCCGAAGCGAGCTTTTTTAATGTGG‑CTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCCCTTACAAGGTAAGCGTCTTGAATAACTTGGCTGGTGTAGGAGGATTCGAACCTCGGAAAGCCGGAATTAGACTTCGGAGCCTTACCGCTTGGCGATACCCCAACAAAATGGTTTTGAATGTGCCGAACATCTTCGATAc                                                      >  1:136330/1‑250 (MQ=37)

                                                                                                                                                                                   gAAGCGAGCTTTTTTAATGTGG‑CTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTGGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGAGATACCCCAAAAAATGGGGTTTGAATTTGGAGTACATATTCGATAGATTCAGa                                               >  1:462415/1‑250 (MQ=38)

                                                                                                                                                                                          gCTTTTTTAATGTGG‑CTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTCCAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGCTTCGACCCTCGGAATGCCGGCATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGACCATATTCGATACATTCAGAATTtcgtg                                       >  1:659558/1‑251 (MQ=38)

                                                                                                                                                                                                      ggg‑CTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCGTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTGGGAATTTGCCGAACATATTCGATTCATTCAGAATTTGGTGGCTACGACGGGa                           <  1:728841/250‑1 (MQ=255)

                                                                                                                                                                                                                  cggggATTCGATCCTCCGAATCCCGGAATTAGAATCCGCTGCCTTCCCGCTTGGCGATACCCCAACTGGGTTCAATTACAAGGTAGGCGTCTTGAATAAAGTGGCTGGGGTACTAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCttt                <  2:397956/248‑3 (MQ=37)

                                                                                                                                                                                                                          ccgatcgcggaagtcctgAATTAGAATCCGGTGCCTTACCGCTTGGCGAGACCCCAACTGGGTGCACTGACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGGACGAGGATTCGAACCGCGGAATGCCGGAATCAGAAGCCGGTGCCTTACCTCTTGGCGATACCCCAACAAATTGGGGTTGAAGTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCca         <  1:368980/233‑1 (MQ=255)

                                                                                                                                                                                                                               cTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCCCTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAATGGTATTGGATTTGCCGAACATATTCGATACATTCAGAATCTGGTGGCTCCGCCGGGCTTCGAACCTGTGAcccactcgtt    >  1:510583/1‑243 (MQ=37)

                                                                                                                                                                                                                                 cGGACTGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGCTCACCCAACTGGGTAGACTTACACGAACAGCGTCTTGCCTAAATTAGCTGAGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGCATCCGGTGCCTTACAGCTTGGCGCTACCCCAACAACTTGGTTTTGAATTTGCCGTACTTATTCGATCCATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCcaagatagg  >  2:368980/1‑243 (MQ=21)

GGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGG‑CTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTAT  >  NC_000913/696232‑696705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`