Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	566,742	A→C	pseudogene (59/93 nt)	renD →	protein RenD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	566,742	0	A	C	98.0%	172.6 / NA	50	pseudogene (59/93 nt)	renD	protein RenD
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base C (23/25);  total (23/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

GGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATACCTGAACCAGTAAAACAACTTCCTGTTATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATTAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGC‑ACAGGACGTTGCCGCGGTAACAGGCGCAACCGTAATCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGT  >  NC_000913/566516‑566904                                                                                                                                                                                                                                    |                                                                                                                                                                   ggccgcagatgagcttgtccatATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTAACAGgcgc                                                                                                                                            <  2:449693/230‑1 (MQ=255)  gccgcagatgagcttgtccatATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAAGCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTAACAGGCGCa                                                                                                                                           <  2:126201/231‑1 (MQ=255)   acgcatatgagctttaccatATGACTGCGAGAATTAACCGTTGTGAGGCGATCCCTGAACCAGTAAAACCACTTACTGTCATGGGCGGTAGACCGCTAAATCGTGCACAGGCTCTGGCGAAGAGTGCAGAAATCAAAGCTAAGTATGGACTGAAAGGAGCAAGTGTATGACGGGCAACGAGGCAATTATTCATTACCTGGGGACGCATAATAGTTTCTGTGC‑GCAGGACGTTGCTGCGCTAACAGGCGCaa                                                                                                                                          <  2:400875/232‑1 (MQ=255)     gcagatgcgcttgtccatATGACTGCGAGACTTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTAACAGGCGCAAc                                                                                                                                         >  2:107521/18‑250 (MQ=255)       agatgagcttgtccatATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTAACAGGCGCAAcagt                                                                                                                                      >  1:591978/16‑248 (MQ=255)        gatgagcttgtccatATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCcgcgcta                                                                                                                                                    >  1:65221/15‑233 (MQ=255)             gcttgtccatatgactgcgAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAcc                                                                                                                                  >  2:575291‑M1/10‑247 (MQ=255)             gcttgtccatatgactgcgAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAcc                                                                                                                                  >  1:507132‑M1/10‑247 (MQ=255)              cttgtccatATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTaa                                                                                                                                                   >  2:276732/9‑231 (MQ=255)              cttgtccatATGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTaa                                                                                                                                                   <  1:276732/223‑1 (MQ=255)               ttgtccatatgactgCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagc                                                                                                                               >  1:318176‑M1/8‑245 (MQ=255)                     ataTGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatc                                                                                                                        <  1:215581‑M1/250‑13 (MQ=255)                     ataTGACTGCGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAAGCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatc                                                                                                                        <  2:722472‑M1/250‑13 (MQ=255)                      tATGACTGCGAGAATTAACCGTGGTGAGGCGATACCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAACGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTAACAGGCGCACCAGTAAccagcataaatc                                                                                                                        >  1:428487‑M1/1‑238 (MQ=255)                              cgagaatTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcgg                                                                                                               <  1:333367‑M1/248‑22 (MQ=255)                                agaATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCCTGGGCGGTAGCCCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggct                                                                                                             >  2:462025‑M1/2‑228 (MQ=255)                                agaATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCGCAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGTAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GTCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggat                                                                                                             <  2:556800‑M1/250‑24 (MQ=255)                                agaATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggct                                                                                                             <  2:314239‑M1/250‑24 (MQ=255)                                agaATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagca                                                                                                                              <  2:518955‑M1/233‑7 (MQ=255)                                agaATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagca                                                                                                                              >  1:518955‑M1/2‑228 (MQ=255)                                  aaTTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcagaaatcaggcggtggctaa                                                                                                           <  2:274002‑M1/251‑26 (MQ=255)                                   aTTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaa                                                                                                           >  1:332450‑M1/1‑225 (MQ=255)                                     tAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaa                                                                                                         <  1:99370‑M1/250‑28 (MQ=255)                                        ccGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggc                                                                                                     >  1:480489‑M1/1‑220 (MQ=255)                                         cGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggc                                                                                                     >  2:332819‑M1/1‑219 (MQ=255)                                            ggtgAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacg                                                                                                  <  2:524069‑M1/250‑35 (MQ=255)                                              tgAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggc                                                                                               >  1:606793‑M1/1‑214 (MQ=255)                                                   cGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtc                                                                                          >  2:29129‑M1/1‑209 (MQ=255)                                                     atccctgaACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtctt                                                                                        <  2:344145‑M1/248‑45 (MQ=255)                                                        ccTGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCGGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctg                                                                                     <  1:496107‑M1/251‑48 (MQ=255)                                                          tGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTCAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTAACAGgcg                                                                                                                                             >  1:198955/1‑193 (MQ=255)                                                          tGAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTAACAGgcg                                                                                                                                             <  2:198955/193‑1 (MQ=255)                                                           gAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctgg                                                                                    <  1:2386‑M1/249‑49 (MQ=255)                                                           gAACCAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcaagggcaggtcttctggtt                                                                                  >  1:216927‑M1/1‑201 (MQ=255)                                                               cAGTAAAACAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctggttatcg                                                                              >  1:331429‑M1/1‑197 (MQ=255)                                                                   aaaaCAACTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctggttatcgaagg                                                                          <  2:369015‑M1/251‑59 (MQ=255)                                                                        aacTTCCTGCCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctggttatcgaaggtaagg                                                                     <  2:216927‑M1/251‑64 (MQ=255)                                                                         acTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCATAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcagggcttctggttatcgaaggtcaggt                                                                    >  1:467128‑M1/1‑187 (MQ=255)                                                                         acTTCCTGTCATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctggttatcgaagttaaggt                                                                    <  1:180954‑M1/251‑65 (MQ=255)                                                                                      ggCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctggttatcgaaggtaaggtctggcgaacggtg                                                       <  1:251491‑M1/251‑78 (MQ=255)                                                                                       gCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCGAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGCGAC‑GGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctggttatcgaaggtaagttctggcgaacggtgt                                                      <  2:638803‑M1/251‑79 (MQ=255)                                                                                                    aaaTCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctggttatcgaaggtaaggtctggcgaacggtgtattaccggtttgc                                         >  2:227532‑M1/1‑160 (MQ=255)                                                                                                     aaTCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctggttatcgaaggtaaggtctggcgaacggtgtattaccggtttgct                                        >  1:729090‑M1/1‑159 (MQ=255)                                                                                                           gCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctggttatcgaaggtaaggtctggcgaacggtgtattaccggtttgctaccag                                   <  2:608027‑M1/250‑98 (MQ=255)                                                                                                                                      atcaaagctAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctggttatcgaaggtaaggtctggcgaacggtgtattaccggtttgctaccagggaagaacgggaaggaaagatgagcacg       <  1:283532‑M1/248‑126 (MQ=255)                                                                                                                                       tcaaagcTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctggttatcgaaggtaaggtctggcgaacggtgtattaccggtttgctaccagggaagaacgggaaggaaagatgagcacg       >  1:473267‑M1/3‑125 (MQ=255)                                                                                                                                        caaagCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctggttatcgaaggtaaggtctggcgaacggtgtattaccggtttgctaccagggaagaacgggaaggaaagatgagcacgaa     <  1:419521‑M1/250‑128 (MQ=255)                                                                                                                                        caaagCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctggttatcgaaggtaaggtctggcgaacggtgtattaccggtttgctaccagggaagaacgggaaggaaagatgagcacga      >  1:416149‑M1/2‑124 (MQ=255)                                                                                                                                         aaaGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctggttatcgaaggtaaggtctggcgaacggtgtattaccggtttgctaccagggaagaacgggaaggaaagatgagcacgaac    <  2:199011‑M1/251‑129 (MQ=255)                                                                                                                                           aGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAATAGCTTCTGTGC‑GCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAAccagcataaatcaggccgcggctaaaatggcacgggcaggtcttctggttatcgaaggtaaggtctggcgaacggtgtattaccggtttgctaccagggaagaacgggaaggaaagatgagcacgaacct  <  1:227532‑M1/251‑131 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                   GGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATACCTGAACCAGTAAAACAACTTCCTGTTATGGGCGGTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATTAAAGCTAAGTTCGGACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCATAAGAGCTTCTGTGC‑ACAGGACGTTGCCGCGGTAACAGGCGCAACCGTAATCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGT  >  NC_000913/566516‑566904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑