Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,587,365	C→T	S139S (AGC→AGT)	mrr →	Type IV methyl‑directed restriction enzyme EcoKMrr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,587,365	0	C	T	100.0%	202.0 / NA	59	S139S (AGC→AGT)	mrr	Type IV methyl‑directed restriction enzyme EcoKMrr
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/32);  total (27/32)

TCGTGCAGGCTGGGCGCATGACCGTTTAAAACGTGCCGGGTTGTCGCAAAGTTTGTCGCGTGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGCCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTT  >  NC_000913/4587119‑4587597                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                         tCGTGCAGGCTGGGCGCATGACCGTTTAAAACGTGCCGGGTTGTCGCAAAGTTTGTCGCGTGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCgg                                                                                                                                                                                                                                      >  1:638426/1‑251 (MQ=255)  cGTGCAGGCTGGGCGCATGACCGTTTAAAACGTGCCGGGTTGTCGCAAAGTTTGTCGCGTGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGTAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCgg                                                                                                                                                                                                                                      <  2:204544/250‑1 (MQ=255)           tGGGCGCATGACCGTTTAAAACGTGCCGGGTTGTCGCACAGTTTGTCGCGTGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGCTCGGtt                                                                                                                                                                                                                            >  2:332572/1‑251 (MQ=255)               cgcATGACCGTTTAAAACGTGCCGGGTTGTCGCAAAGTTTGTCGCGTGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGAt                                                                                                                                                                                                                        <  1:179514/251‑1 (MQ=255)                  aTGACCGTTTAAAACGTGCCGGGTTGTCGCAAAGTTTGTCGCGTGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATc                                                                                                                                                                                                                       >  2:337400/1‑249 (MQ=255)                  aTGACCGTTTAAAACGTGCCGGGTTGTCGCAAAGTTTGTCGCGTGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATc                                                                                                                                                                                                                       >  1:209995/1‑249 (MQ=255)                  aTGACCGTTTAAAACGTGCCGGGTTGTCGCAAAGTTTGTCGCGTGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAg                                                                                                                                                                                                                     <  2:579836/251‑1 (MQ=255)                        gTTTAAAACGTGCCGGGTTGTAGCAAAGTTGGGCGCGTGGCAAAAGGTGCCAGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCAGCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAACGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTa                                                                                                                                                                                                               <  2:62598/251‑1 (MQ=255)                            aaaaCGTGCCGGGTTGTCGCAAAGTTTGTCGCGTGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAag                                                                                                                                                                                                           <  2:527694/251‑1 (MQ=255)                                 gTGCCGGGTTGTCGCAAAGTTTGTCGCGTGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCtt                                                                                                                                                                                                      <  1:73742/251‑1 (MQ=255)                                     cGGGTTGTCGCAAAGTTTGTCGCGTGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTg                                                                                                                                                                                                  >  1:410545/1‑251 (MQ=255)                                                aaaGTTTGTCGCGTGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCt                                                                                                                                                                                       <  2:76330/251‑1 (MQ=255)                                                      tGTCGCGTGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCtgatga                                                                                                                                                                                  <  1:57118/250‑1 (MQ=255)                                                       gTCGCGTGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAgg                                                                                                                                                                                <  1:597271/251‑1 (MQ=255)                                                       gTCGCGTGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATACCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAgg                                                                                                                                                                                >  1:536756/1‑251 (MQ=255)                                                           cgTGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCt                                                                                                                                                                            <  1:488943/251‑1 (MQ=255)                                                                  aaTGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTCGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCACAGAGCAGTCCGGACGATCGGTTAGATCATGCGCTAAAAGAGCTTCGTGATGCGGGGGCTGATGAGGTTCTGGAAAAc                                                                                                                                                                     >  2:43480/1‑251 (MQ=255)                                                                            gACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCa                                                                                                                                                             <  2:327571/249‑1 (MQ=255)                                                                                   gCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTACGTCACGGCCGGCTGCCGTCGATTTAGATCCGCAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTcc                                                                                                                                                    >  1:105000/1‑251 (MQ=255)                                                                                      gtgttggACTGGGTTGCGTCTCATCCCCAGCCCATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTTTGAATGTCAAGCTTAAGTCACGGCCTGATGCCGTCGATTTAGATCCGAAAGCTGACTCTCCCGATCATGAAGAACTTGCAAAGATCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGAGGCGGTTTCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTc                                                                                                                                                 <  2:738845/245‑1 (MQ=255)                                                                                            gACTGGGTTGCGTCTCCTCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCtt                                                                                                                                           >  2:126456/1‑251 (MQ=255)                                                                                               ggggTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGGGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCATGGGCTAAAAGAGCTTCGTGATGCTGTGGCTGATGAGGTGCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGa                                                                                                                                        <  2:105000/250‑1 (MQ=255)                                                                                                gggTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCGCCTTCGCGCTTTGa                                                                                                                                        <  2:96700/250‑1 (MQ=255)                                                                                                                ccAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATg                                                                                                                       <  1:332572/251‑1 (MQ=255)                                                                                                                ccAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATg                                                                                                                       <  1:277590/251‑1 (MQ=255)                                                                                                                          aCGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCg                                                                                                             >  1:141424/1‑251 (MQ=255)                                                                                                                               gCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTgg                                                                                                        <  2:187400/251‑1 (MQ=255)                                                                                                                                cAGGAGACGAAGCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTggg                                                                                                       >  1:57063/1‑251 (MQ=255)                                                                                                                                 aGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTgggg                                                                                                      >  2:396659/1‑251 (MQ=255)                                                                                                                                  ggAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTGCGCGCTTTGAAGTCATTGTTCTGGATGGTTTGCATCGCTTGGGGt                                                                                                     <  2:406322/251‑1 (MQ=255)                                                                                                                                        gAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATggcg                                                                                               >  1:689964/1‑251 (MQ=255)                                                                                                                                            cATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGAGCATGAAGAACTGGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCa                                                                                           <  1:98240/251‑1 (MQ=255)                                                                                                                                                 ggCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGtg                                                                                      >  2:532004/1‑251 (MQ=255)                                                                                                                                                    cTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCCCCGtggtg                                                                                   >  2:527343/1‑248 (MQ=255)                                                                                                                                                     ttCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGtgatg                                                                                   >  2:425814/1‑250 (MQ=255)                                                                                                                                                                gAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCgt                                                                        <  2:84276/250‑1 (MQ=255)                                                                                                                                                                    gTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGGTTCTCCGTCGCGCTTTGAAGTCATTGTTCTGGATGttt                                                                                                                    <  2:295940/202‑1 (MQ=255)                                                                                                                                                                    gTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGATCTGGAAAACTTGTTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGttt                                                                                                                    >  1:295940/1‑202 (MQ=255)                                                                                                                                                                     tCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTggg                                                                  >  2:175092/1‑250 (MQ=255)                                                                                                                                                                        aaCTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGt                                                               <  1:532004/251‑1 (MQ=255)                                                                                                                                                                         aCTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGGTGTTTTGCAGCGTGTTGGCggc                                                               >  2:232622/1‑249 (MQ=255)                                                                                                                                                                             aaGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTg                                                           <  1:51493/250‑1 (MQ=255)                                                                                                                                                                             aaGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGACACCGGGATGATTTGCAGCGTGTTGGCGGTGCTgg                                                          >  2:241995/1‑251 (MQ=255)                                                                                                                                                                                 cACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGAGTGTTGGCGGGACTGgaggt                                                      >  2:236357/1‑249 (MQ=255)                                                                                                                                                                                  aCGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCTCTAAAAGAGCTTCGTTATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGTTATGGCGGCCACCGTGATGATTTGCGGCGTGTTGGCGGTACTGGAGAtg                                                     <  2:55629/251‑1 (MQ=255)                                                                                                                                                                                    gtccGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGCCGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCTTGATGGTTTGCGGGGTGTTGGCGGTACGGgaggtgtt                                                   >  2:146372/3‑246 (MQ=255)                                                                                                                                                                                    ggCCGGCTGCCGTCGTTTTAGATCCGAAAGCCGACTCTCCCTATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGCTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGGATGGCGGCCACCATGATGATTTGCAGCGTGTTGGCGGTACTGGAGAtggt                                                   <  2:390620/251‑1 (MQ=255)                                                                                                                                                                                       cGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGc                                                <  2:209995/251‑1 (MQ=255)                                                                                                                                                                                        ggATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCCCCGGGATGATTTGCAGCGGGTTGGCGGTACTGGAGATGGGGGc                                                >  1:214124/1‑250 (MQ=255)                                                                                                                                                                                              cGTCGAGTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCTGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTGTCTCCTTCGCGCGTTGAAGTCATGGTTCTGGATGTTTTGCATCGCCTGGGGTATGGGGGCCACCTTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATg                                         <  2:72626/251‑1 (MQ=255)                                                                                                                                                                                                              aaaGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGCTCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTCCTGGGGATGGTGGCATCGGTGGGGTGATATCGCTTgt                         >  1:425417/1‑250 (MQ=255)                                                                                                                                                                                                              aaaGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGa                         <  1:337400/251‑1 (MQ=255)                                                                                                                                                                                                                      ctctCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGAGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCCCCGTGATGATTTGCAGCGGGGTGGCGGGACTGGAGGTGGTGGCATTGATGGTGTGATATCGTCTGATAAACTTg                 >  1:62200/1‑251 (MQ=255)                                                                                                                                                                                                                          cccGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGc               <  2:536756/249‑1 (MQ=255)                                                                                                                                                                                                                                cATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGgaga        <  1:126456/250‑1 (MQ=255)                                                                                                                                                                                                                                cATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGGGGCATCGATGGTGTGATATCGCTTGATAAACTTGTCCTGGAGaa       <  2:734581/251‑1 (MQ=255)                                                                                                                                                                                                                                 aTGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGCTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGGGATGATTTGCAGCGTGATGGCGGGACTGGTGGTGGTGGGCTCGGTGGTGTGATATCGCGTGATAAACTTGGACTGGAGaa       >  2:297636/1‑250 (MQ=255)                                                                                                                                                                                                                                   gaagaaCTTGCAAAGCGCAGTCCGGACGATCGGTTAGATCAGGCGCTACAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGGACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGGGAAAGt    >  1:609689/1‑251 (MQ=255)                                                                                                                                                                                                                                     agaaCTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGttt  <  1:581733/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                         TCGTGCAGGCTGGGCGCATGACCGTTTAAAACGTGCCGGGTTGTCGCAAAGTTTGTCGCGTGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGCCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTT  >  NC_000913/4587119‑4587597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑