Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,489,775	C→T	V97M (GTG→ATG)	yjgR ←	DUF853 domain‑containing protein YjgR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,489,775	0	C	T	100.0%	233.4 / NA	69	V97M (GTG→ATG)	yjgR	DUF853 domain‑containing protein YjgR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/34);  total (35/34)

GATGCGCTACTGATATTACCGTACTGATTCTGGAAGGATTTGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACACCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGCCATAAACACCGGCACGCCGATTTCCGACAATGACTCCGCCAGTTTTTGCAGCGTAACGGTTTTACCCGTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTA  >  NC_000913/4489528‑4490014                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                gATGCGCTACTGATATTACCGTACTGATTCTGGAAGGATTTGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCAcatcac                                                                                                                                                                                                                                              >  1:204327/1‑251 (MQ=255) gATGCGCTACTGATACTACCGTACGGATTCTGGAAGGATTTGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGGCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCGCGCCAAAGAGAGCCCAcatcac                                                                                                                                                                                                                                              <  2:446993/251‑1 (MQ=255)     cgcTACTGATATTACCGTACTGATTCTGGAAGGATTTGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACatcaccacc                                                                                                                                                                                                                                          <  2:157821/251‑1 (MQ=255)             atatTACCGTACTGATTCTGGAAGGATTTGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGCTGGCCTTTCTCGCCAACGATATCCCACATCACCACCGGATTGGc                                                                                                                                                                                                                                  >  2:83462/1‑251 (MQ=255)              tatTACCGTACTGATTCTGGAAGGATTTGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCCCATCACCACCGGATTGGc                                                                                                                                                                                                                                  >  2:30591/1‑250 (MQ=255)               atTACCGTACTGATTCTGGAAGGATTTGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCa                                                                                                                                                                                                                                 <  1:236778/250‑1 (MQ=255)                 tACCGTACTGATTCTGGAAGGATTTGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGc                                                                                                                                                                                                                              >  2:574896/1‑251 (MQ=255)                  aCCGTACTGATTCTGGAAGGATTTGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGc                                                                                                                                                                                                                              <  2:478142/250‑1 (MQ=255)                    cGTACTGATTCTGGAAGGATTTGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGACACCGTCGCCCGCACCGGACGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGt                                                                                                                                                                                                                           >  2:247688/1‑251 (MQ=255)                          gATTCTGGAAGGATTTGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGcc                                                                                                                                                                                                                       <  1:649000/249‑1 (MQ=255)                           aTTCTGGAAGGATTTGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGAGTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGt                                                                                                                                                                                                                    <  2:458774/251‑1 (MQ=255)                           aTTCTGCAAGGCTTTGGCGTTATCGACGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCCGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACCATCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCCCCCGCTCCGAATGGCCATTCTCGCCAAAGATATACCACCTCACCACCGGATTGGCATGCGGATGCCAGt                                                                                                                                                                                                                    >  2:316473/1‑251 (MQ=255)                                ggAAGGATTTGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCAttt                                                                                                                                                                                                               >  2:321250/1‑250 (MQ=255)                                  aaGGATTTGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGAc                                                                                                                                                                                                             >  1:171773/1‑251 (MQ=255)                                       tttGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCCGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGCGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCg                                                                                                                                                                                                         >  1:479455/1‑250 (MQ=255)                                        ttGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGa                                                                                                                                                                                                        <  2:533398/250‑1 (MQ=255)                                         tGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGat                                                                                                                                                                                                       <  1:15476/250‑1 (MQ=255)                                          ggCGTTTTCGCCGAGGTACTGGGTAAGTGCCCGCAGATCTGTAAAGTCGAGCAGTAACAGCCCCTGATCGGCAGCAATGCGGAAGATGATATGCAGCACGCCAGATTGCACAGCGTTGAGATGCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGTCGTTTCTCGCCAAAGATATGCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGata                                                                                                                                                                                                      <  1:717916/250‑1 (MQ=255)                                           gCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATAtt                                                                                                                                                                                                    <  1:685967/251‑1 (MQ=255)                                             gTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATAtttt                                                                                                                                                                                                  <  1:354321/251‑1 (MQ=255)                                                aTCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCTCCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTaa                                                                                                                                                                                               <  2:174572/251‑1 (MQ=255)                                                     cGATGTACTGGGTAATTGCCCGCAGATCTTTCAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAGTGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGCTATTTTTAAGCCtt                                                                                                                                                                                          >  2:737827/1‑251 (MQ=255)                                                     cGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGACCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCtt                                                                                                                                                                                          >  1:92708/1‑251 (MQ=255)                                                      gATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTg                                                                                                                                                                                         >  2:43012/1‑251 (MQ=255)                                                       aTGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGCTATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGc                                                                                                                                                                                        >  2:534416/1‑251 (MQ=255)                                                       aTGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGc                                                                                                                                                                                        >  2:610601/1‑251 (MQ=255)                                                                      gCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCg                                                                                                                                                                          <  2:410194/250‑1 (MQ=255)                                                                                  ttAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTcct                                                                                                                                                               <  2:7281/249‑1 (MQ=255)                                                                                        tCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTgcgc                                                                                                                                                       >  1:558780/1‑251 (MQ=255)                                                                                                tAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGCTCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGACGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCgg                                                                                                                                               >  1:100851/1‑251 (MQ=255)                                                                                                 aaCAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGt                                                                                                                                              >  1:244009/1‑251 (MQ=255)                                                                                                     gCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTc                                                                                                                                             >  2:37035/1‑248 (MQ=255)                                                                                                            aTCGTCAGCAATGCGGACGCTGATATTCAGCACGCCAGCTTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGCTGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCtt                                                                                                                                   >  2:674458/1‑251 (MQ=255)                                                                                                              cGTCAGCAATGCGGCAGATGATATTCAGAACGCCAGCTTGCACATCGTTGAGATTCCACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTc                                                                                                                                 >  2:469914/1‑251 (MQ=255)                                                                                                                 cAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTcac                                                                                                                               <  2:185595/250‑1 (MQ=255)                                                                                                                 cAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGGGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTcac                                                                                                                               <  2:211626/250‑1 (MQ=255)                                                                                                                      aTGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGc                                                                                                                         <  2:577802/251‑1 (MQ=255)                                                                                                                         cGGAAGCTGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCAGCACCGGATGGCCTTTCTCGCCCAAGATATACCACATCACCCCCGGATTGGCATGCGGTTGACAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGTCGCCGGTCAGCTCGCCTTTCACATCAGCCAt                                                                                                                      >  2:604087/1‑251 (MQ=255)                                                                                                                           gaagaaGATATTAAGCACGCCCGATCGCATATCGATGAGATTCAACCGTCGTGCCAGCAACAGCGGCCACAGATCCGAAAACGTCGACCGCACCGGATGGCATTACTCGCAAAAGATCTCCCACATCACAACAGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTGAAGCCTTGCGAGCAGTTTTTCCGCCACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGCCATa                                                                                                                     >  2:346144/1‑250 (MQ=255)                                                                                                                            aaGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGGTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGCCATaa                                                                                                                    >  2:562431/1‑250 (MQ=255)                                                                                                                             aGATGATATTCAGCACGCCAGATTGCACATCGTGGAGATTCAACAGGCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGGCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGGTGCCAGTCATTGTCGCCGATATTTTTAAGCCGTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGACTTGCACATCAGCCATAAac                                                                                                                  <  1:671142/251‑1 (MQ=255)                                                                                                                                  atatTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGCCATAAACACCgg                                                                                                             <  2:204327/251‑1 (MQ=255)                                                                                                                                   tatTCAGCACGCCAGCTTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATACCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGCCATAAACACCGGc                                                                                                            >  1:253885/1‑251 (MQ=255)                                                                                                                                     ttCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGGCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCGTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATAGTTTTAAGCCTTGCGAGCAGGTTTTCCGACACCGTTCCTGCCGGCGCGACGCCGGTCAGATCGCCTTTCACAGCAGCCATAAACGCCGGCa                                                                                                           <  2:92708/250‑1 (MQ=255)                                                                                                                                      tCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCCACCGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATAGCCTTTCACATCAGCCATAAACACCGGCACg                                                                                                         >  2:80104/1‑251 (MQ=255)                                                                                                                                          cACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGtttt                                                                                                                                                                              >  1:416507/1‑178 (MQ=255)                                                                                                                                          cACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGACCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGtttt                                                                                                                                                                              <  2:416507/178‑1 (MQ=255)                                                                                                                                             gCCAGATTGCACATCGTTGCGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGCAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGCCATAAACCCCGGCACGCCGAttt                                                                                                  >  2:100396/1‑251 (MQ=255)                                                                                                                                                     gCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGCCATAAACACCGGCACGCCGATTTCCGACAAt                                                                                          <  1:100396/251‑1 (MQ=255)                                                                                                                                                        caTCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGCCATAAACACCGGCACGCCGATTTCCGACAATGAc                                                                                       <  1:253135/251‑1 (MQ=255)                                                                                                                                                                agaTTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACCCCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGCCATAAACACCGGCACGCCGCTTTCCGACAATGACTCCGCCAg                                                                               <  1:235091/251‑1 (MQ=255)                                                                                                                                                                   ttCAACCGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCCCCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGCCCTAAACACCGGCACGCCGATTTCCGACAATGACTCCGCCAGttt                                                                            >  2:503526/1‑251 (MQ=255)                                                                                                                                                                   ttCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGCCATAAACACCGGCACGCCGATTTCCGACAATGACTCCGCCAGttt                                                                            >  1:153276/1‑251 (MQ=255)                                                                                                                                                                          gTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGCCATAAACACCGGCACGCCGATTTCCGACAATGACTCCGCCAGTTTTTGCAGc                                                                     >  2:231431/1‑251 (MQ=255)                                                                                                                                                                            cGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGCCATAAACACCGGCACGCCGATTTCCGACAATGACTCCGCCAGTTTTTGCAGCGt                                                                   <  1:548416/251‑1 (MQ=255)                                                                                                                                                                             gTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGCCATAAACACCGGCACGCCGATTTCCGACAATGACTCCGCCAGTTTTTGCAGCGt                                                                   >  2:201247/1‑250 (MQ=255)                                                                                                                                                                                    cAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCCCATCCCCACCGGATTGGCATGCGGGTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCGGCGCTACGCCGGTCAGATCGCCTTTCACATCAGCCATAAACACCGGCACGCCGATTTCCGACAATGACTCCGCCAGTTTTTGCAGCGTAACGGttt                                                           <  2:605306/251‑1 (MQ=255)                                                                                                                                                                                           ggCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGc                                                                                                                                                  >  1:164200/1‑157 (MQ=255)                                                                                                                                                                                           ggCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGc                                                                                                                                                  <  2:164200/157‑1 (MQ=255)                                                                                                                                                                                                  gATCCGAAACCGTCGCCCGCACCGGAGGTCCTTTCTCGCCAAAGATAGCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGCCATAAACACCGGCACGCCGATTTCCGACAATGACTCCGCCAGTTGTTGCAGCGTAACGGTTCTACCCGTCCCCGtt                                             <  2:100851/251‑1 (MQ=255)                                                                                                                                                                                                         aaCCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCAAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGCCATAAACACCGGCACGCCGATTTCCGACAATGACTCCGCCAGTTTTTGCAGCGTAACGGTTTTACCCGTCCCCGTTGCGCCgg                                      >  1:674224/1‑251 (MQ=255)                                                                                                                                                                                                            cGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGCCATAAACACCGGCACGCCGATTTCCGACAATGACTCCGCCAGTTTTTGCAGCGTAACGGTTTTACCCGTCCCCGTTGCGCCGGTaa                                   <  2:171773/251‑1 (MQ=255)                                                                                                                                                                                                                   cGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGCCATAAACACCGGCACGCCGATTTCCGACAATGACTCCGCCAGTTTTTGCAGCGTAACGGTTTTACCCGTCCCCGTTGCGCCGGTAATCAGccc                            <  1:30591/251‑1 (MQ=255)                                                                                                                                                                                                                        cGGATGGCCTTTCTTGCCAAAGATATCCCACATCCCCACCGGATTAACATGCGGTTGCCAGTCATTGACGCCGATCTTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGCCATAAACACCGGCACGCCGATTTCCGACAATGACTCCGCCAGTTTTTGCAGCGTAACGGTTTTACCCGTCCCCGTTGCGCCGGTAATCAGCCCATgtc                       >  2:490538/1‑249 (MQ=255)                                                                                                                                                                                                                             ggCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGCCATAAACACCGGCACGCCGATTTCCGACAATGACTCCGCCAGTTTTTGCAGCGTAACGGTTTTACCCGTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTa                  <  1:151568/251‑1 (MQ=255)                                                                                                                                                                                                                              gCCTTTCTCGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGAGAGTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGCCATAAACACCGGCACGCCTATTTCCGACAATGACTCCGCCAGTTTGTGCAGCGTAACGGTTTTACCCGTCCCCGTTGCGCCGGTAATCAGCCCATGACGGtgag                 <  2:349340/251‑4 (MQ=255)                                                                                                                                                                                                                                   tctcGCCAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGCCATAAACACCGGCACGCCGATTTCCGACAATGACTCCGCCAGTTTTTGCAGCGTAACGGTTTTACCCGTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCAt             <  1:48940/250‑1 (MQ=255)                                                                                                                                                                                                                                     ccGCCAAAGATATCCCATATCACCACCGTATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTGCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGCCATAAACTCCGGCACGCCGATGTCCGACAATGACTCCGCCAGTTTTTGCAGCGGAACGGGTTTACTCGTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTcc          <  1:316473/250‑1 (MQ=255)                                                                                                                                                                                                                                      cGCCAAAGATATCCCGCATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCTCATCAGCCATAAACACCGGCACGCCGATTTCCGACAATGACTCCGCCAGTTTTTGCAGCGTAACGGTTTTACCCGTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTccc         <  1:737827/251‑1 (MQ=255)                                                                                                                                                                                                                                        acAAAGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGCCATAAACACCGGCACACCGATTTCCGACAATGACTCCGCCAGTTTTTGCAGCGTAACGGTTTTACCCGTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCgg       >  2:233650/2‑251 (MQ=255)                                                                                                                                                                                                                                            aGATATCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGCCATAAACACCGGCACGCCGATTTCCGACAATGACTCCGCCAGTTTTTGCAGCGTAACGGTTTTACCCGTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGt   <  1:277144/251‑1 (MQ=255)                                                                                                                                                                                                                                              atatCCCACATCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGCCATAAACACCGGCACGCCGATTTCCGACAATGACTCCGCCAGTTTTTGCAGCGTAACGGTTTTACCCGTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTa  >  1:19802/1‑250 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                GATGCGCTACTGATATTACCGTACTGATTCTGGAAGGATTTGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACACCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTTGCGAGCAGTTTTTCCGACACCGTTCCTGCCTGCGCGACGCCGGTCAGATCGCCTTTCACATCAGCCATAAACACCGGCACGCCGATTTCCGACAATGACTCCGCCAGTTTTTGCAGCGTAACGGTTTTACCCGTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTA  >  NC_000913/4489528‑4490014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑