BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,482,901	C→T	V313M (GTG→ATG)	valS ←	valine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,482,901	0	C	T	99.1%	385.6 / NA	108	V313M (GTG→ATG)	valS	valine‑‑tRNA ligase
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (48/59); minor base G (0/1); total (48/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

AACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACACCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCC  >  NC_000913/4482656‑4483144

aaCCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTggg                                                                                                                                                                                                                                                >  1:604663/1‑251 (MQ=255)

aaCCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTggg                                                                                                                                                                                                                                                <  1:479844/251‑1 (MQ=255)

 aCCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGgc                                                                                                                                                                                                                                               >  1:87009/1‑251 (MQ=255)

   cgccgGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGgcgc                                                                                                                                                                                                                                             >  1:174130/1‑251 (MQ=255)

    gccgGTTTCGCCAGGACATCGGCAAGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTAGCCGTAAGGAACGGTCAGGTCGAGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGACAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGGTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGGATCGAACATCTGGGCGCt                                                                                                                                                                                                                                            >  2:738965/1‑251 (MQ=255)

      cgGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCttt                                                                                                                                                                                                                                          <  1:569604/251‑1 (MQ=255)

            cGCCAGGACATCGGCACGCACGTTCCACGGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTTGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACgt                                                                                                                                                                                                                                     <  2:498364/250‑2 (MQ=255)

             gCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGat                                                                                                                                                                                                                                   <  2:577326/251‑1 (MQ=255)

               cAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGatat                                                                                                                                                                                                                                 >  1:597239/1‑251 (MQ=255)

                aGGACCTCGGCGCGCACGTACCACTGGTCGGTCTGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGataac                                                                                                                                                                                                                                >  2:436709/1‑249 (MQ=255)

                aGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATc                                                                                                                                                                                                                                <  2:645572/251‑1 (MQ=255)

                 ggACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTACTTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCg                                                                                                                                                                                                                               >  2:730960/1‑251 (MQ=255)

                 ggACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCg                                                                                                                                                                                                                               >  2:556303/1‑251 (MQ=255)

                     aTCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTACAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGt                                                                                                                                                                                                                           >  1:728571/1‑251 (MQ=255)

                           acgcacgTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAgg                                                                                                                                                                                                                     >  1:191620/1‑251 (MQ=255)

                            cgcacgTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGt                                                                                                                                                                                                                    <  2:383598/251‑1 (MQ=255)

                             gcacgTACCACTGGTCGGTCAGCATTGGTGCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACGCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGGCAAAGGTc                                                                                                                                                                                                                   <  2:727122/251‑1 (MQ=255)

                                      ccTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTg                                                                                                                                                                                                          <  2:667586/250‑1 (MQ=255)

                                       cTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGAGGTTTAATTTCTTCCAGCAGGCCCAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTgt                                                                                                                                                                                                         >  2:327064/1‑250 (MQ=255)

                                            cggtcAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGatcat                                                                                                                                                                                                     <  1:599089/250‑1 (MQ=255)

                                             ggtcAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCg                                                                                                                                                                                                   <  2:237792/251‑1 (MQ=255)

                                                   cATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAggg                                                                                                                                                                                             <  1:375422/251‑1 (MQ=255)

                                                       ggTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGt                                                                                                                                                                                          <  2:714231/250‑1 (MQ=255)

                                                         ttCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGa                                                                                                                                                                                        <  2:440969/250‑1 (MQ=255)

                                                          tCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGAc                                                                                                                                                                                       <  1:234807/250‑1 (MQ=255)

                                                             aTAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTTACGttt                                                                                                                                                                                   <  2:101891/251‑1 (MQ=255)

                                                                   ccgccgcCACGGTCGCCGTAAGGAACGGGCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTAccc                                                                                                                                                                               <  2:283175/248‑1 (MQ=255)

                                                                      ccgccACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACATGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTc                                                                                                                                                                          >  2:112722/1‑251 (MQ=255)

                                                                        gccACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCAt                                                                                                                                                                        <  1:606955/251‑1 (MQ=255)

                                                                           aCGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACTACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGt                                                                                                                                                                     <  1:421768/251‑1 (MQ=255)

                                                                              gTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGt                                                                                                                                                                  >  1:30221/1‑251 (MQ=255)

                                                                                 gCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCGTTGGTATCGAACATCTGGGCGCTGTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTaa                                                                                                                                                               <  1:340332/251‑1 (MQ=255)

                                                                                  ccGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCCAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACCTCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGCTGTTGATCATCGGCAGGGCGTGACGTTTACCCCCTTCCTAGTCGttca                                                                                                                                                               >  2:349762/1‑248 (MQ=255)

                                                                                   cGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTaa                                                                                                                                                               >  2:645919/1‑249 (MQ=255)

                                                                                      aaGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCg                                                                                                                                                          >  2:576077/1‑251 (MQ=255)

                                                                                         gAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTgc                                                                                                                                                       <  1:270611/251‑1 (MQ=255)

                                                                                          aaCGGTCAGGCCGTGAGGATTAATTTCTTCCACAAGGCCACGCCAGTCCACCGCGGCACCGGCTGCTTTAGGTGCAGCAACACGCTCAAGTTCCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTAAGATTCGTTACCTTTGGTCTCGAACTTCTGGGCGCCTCCCCGGATATCGCCGTCAAAGGTCAGGATGTTGTTCATCGCCCGGCCGTGACGTTTACCCCCTTCATAGTCGTTAAAGTCGTgcg                                                                                                                                                      >  2:456856/1‑251 (MQ=255)

                                                                                             ggTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCg                                                                                                                                                   >  1:116625/1‑251 (MQ=255)

                                                                                             ggTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCg                                                                                                                                                   <  1:551088/251‑1 (MQ=255)

                                                                                              gTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCCGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCgg                                                                                                                                                  >  2:549319/1‑251 (MQ=255)

                                                                                                  ggTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGGCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCGTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGAACATCGGCAGGGCGTGACGTTTACCCACGGCATAGTCGTTAAAGTCGTGCGCCGGAGTg                                                                                                                                              <  2:298947/251‑1 (MQ=255)

                                                                                                          ggTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCAc                                                                                                                                      <  2:687876/251‑1 (MQ=255)

                                                                                                             ttAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGc                                                                                                                                    <  2:257781/250‑1 (MQ=255)

                                                                                                              tAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCa                                                                                                                                   <  2:116625/250‑1 (MQ=255)

                                                                                                               aaTTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGc                                                                                                                                 <  2:352978/251‑1 (MQ=255)

                                                                                                                aTTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCAGCGGCAGGGCGTGACGTTTACCCACGTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGcc                                                                                                                                <  2:64181/251‑1 (MQ=255)

                                                                                                                 tttCTTCCAGCCGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTACAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAGTAAACGTCAGATTAGTTACCTTTGGTATCGACCATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTAAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCCTAGGCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCg                                                                                                                               >  1:486946/1‑251 (MQ=255)

                                                                                                                  ttcttacagacggCCAAGAGCGTCAAGTGCGGCAACGGCTGCTTTACGTGCAGGAAAACGCTCCCGTTTCTAGAACTCGGCAGGGCTTTCGCTGTAATAAACGTCAGATTCGTTACCTTTGGTATCGAACCTCTGGGCGCTTTCACGGATCTAGCCGTCAAAGGTCAGGATGTTGAACATCGGCAGGGCGTGACGTTTACCCACTTCTCAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCgg                                                                                                                              >  2:692992/12‑251 (MQ=255)

                                                                                                                   tcttcCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGt                                                                                                                             >  1:385189/1‑251 (MQ=255)

                                                                                                                       ccAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCCCTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCt                                                                                                                         >  2:25320/1‑251 (MQ=255)

                                                                                                                        cagcagGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCtt                                                                                                                        >  1:430645/1‑251 (MQ=255)

                                                                                                                        cagcagGCCAAGCGCGTCAACGGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCGGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTGCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCAGAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCtt                                                                                                                        <  1:650458/251‑1 (MQ=255)

                                                                                                                         agcagGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCttt                                                                                                                       >  2:558009/1‑251 (MQ=255)

                                                                                                                                cAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATg                                                                                                                >  2:113625/1‑251 (MQ=255)

                                                                                                                                  aGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGTAGCCGGTGCCTTTTTCCATGTc                                                                                                              >  1:545615/1‑251 (MQ=255)

                                                                                                                                  aGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTc                                                                                                              <  2:174130/251‑1 (MQ=255)

                                                                                                                                   gcgcGTCAACGGCGGCAACGACAGCTGTACGTGCAGCAAAACGCTCCAGTTTCTGGAACGCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGCTACCTTTGGTATCGAACATCTGGGCGCTGTCACGGATATCGCCGCCAAAGGTCAGGATGTTTATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGGGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCg                                                                                                             <  1:286372/251‑1 (MQ=255)

                                                                                                                                    cgcgTCAACTGCGGCAATGACTGCTTTACGTGCACCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGTTTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACACACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCgg                                                                                                            >  2:667746/1‑251 (MQ=255)

                                                                                                                                          aaCTGCGGCAACGACTTCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTAGCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCAGGTCGGCGTGtt                                                                                                      <  2:604663/251‑1 (MQ=255)

                                                                                                                                          aaCTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTGCGCTGGAATAAACGTCAGATTCGTTACCTTTTGTATCGAACATCTGGGCGCTTTCACGGATATCTCCGTCAAAGGTCAGGATTTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTTCCTTTTTCCATGTCTGCGTGtt                                                                                                      <  1:101546/251‑1 (MQ=255)

                                                                                                                                          aaCTGCCGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGAGCATCGGCAGGGCTTGACGTTTACCCACTTCATAGTCGTTAAAGGCGTTCGCCGGAGTGAGGTTCACGCAGCCTGTGCCTGTGTCCATGTCGGCGTGtt                                                                                                      <  2:700918/251‑1 (MQ=255)

                                                                                                                                           aCTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCGCCAGTTTTTGGAACTCTGCAGGGATTTCGGTGGAATAAACGTCAGATTCGTTAACTTTGGTATCGAACATCTGGGCGCTTTCACTGATATCGCCGTCAAAGTTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACGTCATAGTCGTTAAAGTCGTGTGCCGGAGTGATTTTAACGCAGCCGGTGCCTTTTTCCATGTCGGCgtgtac                                                                                                     <  1:692992/251‑3 (MQ=255)

                                                                                                                                                ggCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGc                                                                                                >  2:538415/1‑251 (MQ=255)

                                                                                                                                                   aaCGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCa                                                                                              >  1:307390/1‑250 (MQ=255)

                                                                                                                                                     cGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACg                                                                                           >  2:374892/1‑251 (MQ=255)

                                                                                                                                                      gACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACg                                                                                           >  1:200321/1‑250 (MQ=255)

                                                                                                                                                          gCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCATGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATct                                                                                       <  2:145503/250‑2 (MQ=255)

                                                                                                                                                          gCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATc                                                                                        <  1:730960/249‑1 (MQ=255)

                                                                                                                                                          gCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATAGCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCgg                                                                                      <  1:645919/251‑1 (MQ=255)

                                                                                                                                                          gCTTTACGGGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTTACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATc                                                                                        <  1:327064/249‑1 (MQ=255)

                                                                                                                                                             ttACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGaa                                                                                    <  1:52950/250‑1 (MQ=255)

                                                                                                                                                             ttACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGGTCGTCGCCAACGATCGGa                                                                                     <  1:556303/249‑1 (MQ=255)

                                                                                                                                                               aCGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATa                                                                                  <  1:374892/250‑1 (MQ=255)

                                                                                                                                                               aCGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGGTACCTTTGGTATCGAACATCTGGGCGCTGTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATa                                                                                  <  2:687707/250‑1 (MQ=255)

                                                                                                                                                                cGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGTGCTTTCACGTATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTTACGTTTACCCACTTCATAGTCGTTAAATTCGTGCGCCGGAGTGATTTTCACGCAGCCTGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATacg                                                                                <  1:434182/251‑1 (MQ=255)

                                                                                                                                                                    cagcaAAACGCTCCAGTTTATGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTGCCTTTGGTATCGAACATCTGGTCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACgg                                                                            <  1:349762/251‑1 (MQ=255)

                                                                                                                                                                       caAAACTCTCTAGTTTCTGGAACTCTGCATGGATTTCCCTGCCATACACGTCAGCTTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGCGACGTTTACCCCCTTCTTAGTCGTTAAAGTCGTGCGCCGGCGGGCTTTTCACGCAGCCGGCGCCTTTTTCCATGTCGGCGCATTCGTCTCCAACGATCGGagtccgacagttt                                                                         >  2:301253/1‑239 (MQ=255)

                                                                                                                                                                        aaaaCGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTaa                                                                        <  2:191620/251‑1 (MQ=255)

                                                                                                                                                                              cTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCg                                                                  >  1:431919/1‑251 (MQ=255)

                                                                                                                                                                                   gTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGTCGGTTAACCAGCGGCAGa                                                             <  2:133499/251‑1 (MQ=255)

                                                                                                                                                                                     tgcTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGaa                                                            <  2:728571/248‑1 (MQ=255)

                                                                                                                                                                                         ggAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGTCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGa                                                         <  1:665035/249‑1 (MQ=255)

                                                                                                                                                                                           aaCTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGtt                                                                                                                                                                 <  1:52605/143‑1 (MQ=255)

                                                                                                                                                                                               ctGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATAtt                                                   <  2:732491/249‑1 (MQ=255)

                                                                                                                                                                                                   aGGGAGTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGGGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACCAATTTGCCa                                              >  2:472157/1‑250 (MQ=255)

                                                                                                                                                                                                     ggATTTCGCTGGAATAAACGTCACATTCGTTCCCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGTCGTCAAAGGTCAGGAGGTTGATCATCGGCAGGGCGTGACGGTTTCCCACTTCATAGTCGCTAAAGTCGTGCGCCGGAGTGATTTTCGCGCAGCCGGTGTCTTTTTCCATGTCGGCGTGTTCGTCGCGAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATc                                           <  1:729678/251‑1 (MQ=255)

                                                                                                                                                                                                      gATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGGATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCa                                          <  2:430645/251‑1 (MQ=255)

                                                                                                                                                                                                          tCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATc                                           >  2:485812/1‑246 (MQ=255)

                                                                                                                                                                                                          gcGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGAt                                       <  2:597239/249‑1 (MQ=255)

                                                                                                                                                                                                               ggAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTg                                  <  2:447429/250‑1 (MQ=255)

                                                                                                                                                                                                                gAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGt                                 >  1:132976/1‑250 (MQ=255)

                                                                                                                                                                                                                    aaaCGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAAcgc                            >  2:642651/1‑251 (MQ=255)

                                                                                                                                                                                                                     aaCGTCAGATTCGTTACCTTTGGTATCGTACATCTGGGAGCTTTCACGGAAATCGCCGTCAAAGGTCAGGACGATGATCATCGGCAGGACGTGACGCTTACCCACTTCCTAGACGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCCGCCGGTGCCCTTTTCTCTGTCGGCGTGTTCGTCGCCAACGATAGGCATACGACGGCTAACCCGCCGCAGCATGCCATCGTTGCCCATCCGATCTTTGGAAcgcg                           >  2:220315/1‑251 (MQ=255)

                                                                                                                                                                                                                     aaCGTCAGATTCGTTACCGTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCTCTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAAcgct                           <  2:556280/251‑2 (MQ=255)

                                                                                                                                                                                                                     aaCGGCAGATTCGTTACCTGTGGTATCGAACATATGGGCGCTTTCACGAATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGGTCGTCGCCAACGGTCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAAcgcg                           >  1:539368/1‑251 (MQ=255)

                                                                                                                                                                                                                         tCAGATTCGTTACCTTTGGTATCGAACATCTGGACGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGCTCGGAATACGAAGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATc                       >  1:583899/1‑251 (MQ=255)

                                                                                                                                                                                                                               tCGTTACCTTTGGTATCGAACATCTGGGCGCCTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTGTACCCACGTCATAGTCGTTAAAGTCGGGCGCTGGAGTGAGTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCg                  <  1:456856/250‑1 (MQ=255)

                                                                                                                                                                                                                                    aCCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTa             >  2:479464/1‑250 (MQ=255)

                                                                                                                                                                                                                                     ccTTTAGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCACAGGTCAGGATGTTGATCATCGGCAGGGCGTGCCGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGGGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAAc           >  2:517416/1‑251 (MQ=255)

                                                                                                                                                                                                                                        ttGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACg          <  1:112722/249‑1 (MQ=255)

AACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACACCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCC  >  NC_000913/4482656‑4483144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`